Anas Raklami, Olubukola Oluranti Babalola, Martin Jemo, Ahmed Nafis
In this study, we successfully isolated two distinct yeasts from Moroccan extreme environments. These yeasts were subjected to molecular characterization by analyzing their ITS regions. Our research thoroughly characterizes plant growth-promoting (PGP) abilities and their drought and salt stress tolerance. In a greenhouse assay, we examined the impact of selected yeasts on Medicago sativa's growth. Four treatments were employed: i) control without inoculation (NI), ii) inoculation with L1, iii) inoculation with L2, and iv) inoculation with the mixture L1+L2...
February 28, 2024: FEMS Microbiology Letters
Hongju Daisy Chen, Lianwei Li, Fubing Yu, Zhanshan Sam Ma
Autism spectrum disorder (ASD) is estimated to influence as many as 1% children worldwide, but its etiology is still unclear. It has been suggested that gut microbiomes play an important role in regulating abnormal behaviors associated with ASD. A de facto standard analysis on the microbiome-associated diseases has been diversity analysis, and nevertheless, existing studies on ASD-microbiome relationship have not produced a consensus. Here, we perform a comprehensive analysis of the diversity changes associated with ASD involving alpha-, beta-, and gamma-diversity metrics, based on 8 published datasets consisting of 898 ASD samples and 467 healthy controls (HC) from 16S-rRNA sequencing...
February 28, 2024: FEMS Microbiology Letters
Rino Isshiki, Hirotsugu Fujitani, Satoshi Tsuneda
Ammonia-oxidizing bacteria, key players in the nitrogen cycle, have been the focus of extensive research. Numerous novel species have been isolated and their growth dynamics were studied. Despite these efforts, controlling their growth to obtain diverse physiological findings remains a challenge. These bacteria often fail to grow, even under optimal conditions. This unpredictable growth pattern could be viewed as a survival strategy. Understanding this heterogeneous behavior could enhance our ability to culture these bacteria...
February 23, 2024: FEMS Microbiology Letters
Yuqing Bai, Wei Zhang, Ruonan Guo, Jiyuan Yu, Yurong Wang
Monascus pigments (MPs) are a kind of natural ingredient fermented by Monascus spp., which contains three types of pigments: red, orange and yellow ones. Monascus yellow pigments have a restricted yield and cannot meet industrial application. The method and mechanism of CaCl2 improving yellow pigments production by liquid fermentation of M. purpureus M8 were studied in order to overcome the low yield of yellow pigments produced by liquid fermentation. Changes in physiological and biochemical indicators explained the effects of CaCl2 on the production of Monascus yellow pigments from solid fermentation...
February 20, 2024: FEMS Microbiology Letters
Xinshu Zhu, Yongcui Deng, Yongqin Liu
Methane-oxidizing bacteria (methanotrophs) play an important role in mitigating methane emissions in various ecological environments, including cold regions. However, the response of methanotrophs in these cold environments to extreme temperatures above the in-situ temperature has not been thoroughly explored. Therefore, this study collected soil samples from Longxiazailongba (LXZ) and Qiangyong (QY) glacier forelands and incubated them with 13CH4 at 35°C under different soil water conditions. The active methanotroph populations were identified using DNA stable isotope probing (DNA-SIP) and high throughput sequencing techniques...
February 16, 2024: FEMS Microbiology Letters
Fabio Dal Bello, Laetitia Bocquet, Audrey Bru, Svend Laulund, Ronnie Machielsen, Matteo Raneri, Vincent Sewalt, Noël van Peij, Patrice Ville, Federica Volonté, Yolanda White, Jakub Rusek
Non-transgenic New Genomic Techniques (NGTs) have emerged as a promising tool for food industries, allowing food cultures to contribute to an innovative, safe and more sustainable food system. NGTs have the potential to be applied to microorganisms, delivering on challenging performance traits like texture, flavour and an increase of nutritional value. This paper brings insights on how non-transgenic NGTs applied to food cultures could be beneficial to the sector, enabling food industries to generate innovative, safe and sustainable products for European consumers...
February 6, 2024: FEMS Microbiology Letters
Hera Karayanni, Eleni Motsiou, Vasiliki Sapountzi, Lydia Meggou, Maria Pagkoutsou, Aikaterini Triantafyllidi, Alexandra-Kyparisia Markouti, Sevasti Zervou, Stelios Anastasopoulos, George Efthimiou
Microbes are ubiquitous and provide numerous services to humans and our planet. However, a query arises as to whether these microbial services are valued by the general public especially after unprecedented conditions like the COVID-19 pandemic. In this context a survey was conducted to investigate the concept of microbe in Greece. Thematic analysis of 672 anonymous responses (age range 4-75yo) received for the open-ended prompt "What is the first thing that comes to mind when you hear the word microbe?" revealed five thematic categories: Negative emotions, Fuzzy associations, Biology, Entities and Health...
February 1, 2024: FEMS Microbiology Letters
Tomonori Kano, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito
Colistin is a cationic cyclic antimicrobial peptide used as a last resort against multidrug-resistant gram-negative bacteria. To understand the factors involved in colistin susceptibility, we screened colistin-sensitive mutants from an E. coli gene-knockout library (Keio collection). The knockout of purA, whose product catalyzes the synthesis of adenylosuccinate from IMP in the de novo purine synthesis pathway, resulted in increased sensitivity to colistin. Adenylosuccinate is subsequently converted to AMP, which is phosphorylated to produce ADP, a substrate for ATP synthesis...
February 1, 2024: FEMS Microbiology Letters
Meganathan P Ramakodi
A comprehensive profiling of microbial diversity is essential to understand the ecosystem functions. Universal primer sets such as the 515Y/926R could amplify a part of 16S and 18S rRNA and infer the diversity of prokaryotes and eukaryotes. However, the analyses of mixed sequencing data pose a bioinformatics challenge; the 16S and 18S rRNA sequences need to be separated first and analysed individually/ independently due to variations in the amplicon length. This study describes an alternative strategy, a merging and concatenation workflow, to analyse the mixed amplicon data without separating the 16S and 18S rRNA sequences...
February 1, 2024: FEMS Microbiology Letters
Kohtetsu Kita, Momotaka Uchida, Tsutomu Arie, Tohru Teraoka, Hisatoshi Kaku, Yasukazu Kanda, Masaki Mori, Takayuki Arazoe, Takashi Kamakura
Rice blast fungus (Pyricularia oryzae) is a heterothallic ascomycete that causes the most destructive disease in cultivated rice worldwide. This fungus reproduces sexually and asexually, and its mating type is determined by the MAT1 locus, MAT1-1 or MAT1-2. Interestingly, most rice-infecting field isolates show a loss of female fertility, but the MAT1 locus is highly conserved in female-sterile isolates. In this study, we performed a functional analysis of MAT1 using the CRISPR/Cas9 system in female- and male-fertile isolates and female-sterile (male-fertile) isolates...
February 1, 2024: FEMS Microbiology Letters
Fuka Yamazaki, Kyosuke Kobayashi, Junko Mochizuki, Toshihiro Sashihara
Human induced pluripotent stem cell-derived small intestinal epithelial cell (hiPSC-SIEC) monolayers are useful in vitro models for evaluating the gut mucosal barrier; however, their reactivity to cytokines, which are closely related to the regulation of mucosal barrier function, remains unclear. Interleukin (IL)-22 is a cytokine that contributes to regulate the mucosal barrier in the intestinal epithelia. Using microarray and Gene Set Enrichment Analysis, we found that hiPSC-SIEC monolayers activate the immune response and enhance the mucosal barrier in response to IL-22...
January 24, 2024: FEMS Microbiology Letters
Paula Maria Moreira Martins, Laís Moreira Granato, Túlio Morgan, Julia Lopes Nalin, Marco Aurélio Takita, Poliane Alfenas-Zerbini, Alessandra Alves de Souza
Xanthomonas is an important genus of plant-associated bacteria that causes significant yield losses of economically important crops worldwide. Different approaches have assessed genetic diversity and evolutionary interrelationships among the Xanthomonas species. However, information from clustered regularly interspaced short palindromic repeats (CRISPR) has yet to be explored. In this work, we analyzed the architecture of CRISPR-Cas loci and presented a sequence similarity-based clustering of conserved Cas proteins in different species of Xanthomonas...
January 19, 2024: FEMS Microbiology Letters
Joo-Han Gwak, Sung-Keun Rhee, Joong-Wook Park
Tarballs are formed from released or discharged crude oil containing sulfur compounds. A considerable amount and variety of sulfate-reducing bacteria (SRB) and sulfur-oxidizing bacteria (SOB) were identified in tarballs collected from the intertidal and supratidal zones of Alabama's Gulf beaches. Amplicon sequencing of the bacterial 16S rRNA gene showed that SRB were more abundantly distributed in the core than on the surface of tarballs, while no significant differences were observed in the distribution of SOB...
January 16, 2024: FEMS Microbiology Letters
Jiamin Yang, Mingxi Zeng, Hui Wu, Zhenlin Han, Zhiyan Rock Du, Xiaobin Yu, Wei Luo
CrgA has been shown to be a negative regulator of carotenogenesis in some filamentous fungi, while light irradiation is an inducible environmental factor for carotenoid biosynthesis. To clarify the relationship between CrgA and light-inducible carotenogenesis in Blakeslea trispora, the cis-acting elements of the btcrgA promoter region were investigated, followed by the analyses of correlation between the expression of btcrgA and carotenoid structural genes under different irradiation conditions. A variety of cis-acting elements associated with light response was observed in the promoter region of btcrgA, and transcription of btcrgA and carotenoid structural genes under different irradiation conditions was induced by white light with a clear correlation...
January 10, 2024: FEMS Microbiology Letters
Jae Kyu Lim
Reduction of CO2 to formate utilizing formate dehydrogenases has been attempted biologically and electrochemically. However, the conversion efficiency is very low due to the low energy potential of electron donors and/or electron competition with other electron acceptors. To overcome such a low conversion efficiency, I focused on a direct electron transfer between two unrelated redox enzymes for the efficient reduction of CO2 and utilized quantum mechanical magnetic properties of the [Fe-S] cluster to develop a novel electron path...
January 9, 2024: FEMS Microbiology Letters
Shuang Wang, Manik Prabhu Narsing Rao, Syed Raziuddin Quadri
In this study, a genomic approach was employed to evaluate the metabolic potentials and taxonomic classification of the halophilic genus Halarchaeum. Genomic analysis revealed that Halarchaeum members exhibit a predilection for amino acids as their primary energy source in high-salinity environments over carbohydrates. Genome analysis unveiled the presence of crucial genes associated with metabolic pathways, including the Embden-Meyerhof pathway, semi-phosphorylative Entner-Doudoroff pathway, and the urea cycle...
January 8, 2024: FEMS Microbiology Letters
Christos Giannakopoulos, Manthos Panou, Spyros Gkelis
The characterization of cyanobacteria communities remains challenging, as taxonomy of several cyanobacterial genera is still unresolved, especially within Nostocales taxa. Nostocales cyanobacteria are capable of nitrogen fixation; nitrogenase genes are grouped into operons and are located in the same genetic locus. Structural nitrogenase genes (nifH, nifK and nifD) as well as 16 s rRNA have been shown to be adequate genetic markers for distinguishing cyanobacterial genera. However, there is no available information regarding the phylogeny of regulatory genes of the nitrogenase cluster...
January 2, 2024: FEMS Microbiology Letters
Tatev Manutsyan, Syuzanna Blbulyan, Anait Vassilian, Tatiana Semashko, Gayane Kirakosyan, Lilit Gabrielyan, Karen Trchounian, Anna Poladyan
Ralstonia eutropha is a facultative chemolithoautotrophic aerobic bacterium that grows using organic substrates or H2 and CO2. Hydrogenases (Hyds) are synthesized under lithoautotrophic, or energy-limited heterotrophic conditions and are used in enzyme fuel cells (EFC) as anodic catalysts. The effects of chemically synthesized gold nanoparticles (Au-NPs) on R. eutropha H16 growth, oxidation-reduction potential (ORP) kinetics, and H2-oxidizing Hyd activity were investigated in this study. Atomic force microscopy showed that thin, plate-shaped Au-NPs were in the nanoscale range with an average size of 5...
January 2, 2024: FEMS Microbiology Letters
Sijie Wang, Ming Su, Xueqian Hu, Xiaocheng Wang, Qian Han, Qiaoling Yu, Petr Heděnec, Huan Li
Invertebrates such as termites feeding on nutrient-poor substrate receive essential nitrogen by biological nitrogen fixation of gut diazotrophs. However, the diversity and composition of gut diazotrophs of vertebrates such as Plateau pikas living in nutrient-poor Qinghai-Tibet Plateau remain unknown. To fill this knowledge gap, we studied gut diazotrophs of Plateau pikas (Ochotona curzoniae) and its related species, Daurian pikas (Ochotona daurica), Hares (Lepus europaeus) and Rabbits (Oryctolagus cuniculus) by high-throughput amplicon sequencing methods...
December 20, 2023: FEMS Microbiology Letters
Achiraya Siriphap, Watsawan Prapasawat, Jednipit Borthong, Wimonrat Tanomsridachchai, Chonchanok Muangnapoh, Orasa Suthienkul, Kaknokrat Chonsin
Vibrio parahaemolyticus (V. parahaemolyticus) is commonly found in seawater and seafood products, but evidence is limited of its presence in seafood marketed in locations very distant from coastal sources. This study determined the prevalence and characterization of V. parahaemolyticus in seafood from markets in landlocked Phayao province, Northern Thailand. Among 120 samples, 26 (21.7%) were positive for V. parahaemolyticus, being highest in shrimp (43.3%), followed by shellfish (36.7%), and squid (6.7%), but was not found in fish...
December 18, 2023: FEMS Microbiology Letters
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