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FEMS Microbiology Letters

Hyesuk Seo, Ti Lu, Rahul M Nandre, Qiangde Duan, Weiping Zhang
Enterotoxigenic Escherichia coli (ETEC) producing type Ib heat-stable toxin (STa) are a top cause of children's diarrhea and travelers' diarrhea, thus STa needs to be targeted in ETEC vaccine development. However, since this 19-amino acid STa is poorly immunogenic, the effort to genetically fuse or chemically couple to carrier proteins has been made to enhance STa immunogenicity. In this study, we selected one genetic fusion and one chemical conjugate to comparatively evaluate STa immunogenicity. The genetic fusion is 3xSTaN12S-mnLTR192G/L211A carrying three toxoid (STaN12S) genetically fused to a double mutant LT monomer (mnLTR192G/L211A); the chemical conjugate is BSA-STaA14T which has toxoid STaA14T chemically coupled to bovine serum albumin (BSA)...
February 17, 2019: FEMS Microbiology Letters
Sergey V Bazhenov, Svetlana A Khrulnova, Maria N Konopleva, Ilya V Manukhov
Here we present a study of luminescent intestinal microflora of the fish inhabiting Bering and Okhotsk seas in summer and winter seasons. Sampling of intestinal luminescent microflora was carried for several years, with all recovered species belonging to psychrophilic bacteria of either Aliivibrio logei or Photobacterium phosphoreum species. A seasonal change in fish intestinal luminescent microflora detected include an increase in prevalence of P. phosphoreum bacteria in summer and an increase in prevalence of A...
February 17, 2019: FEMS Microbiology Letters
Alok Kumar Mishra, Shravanthi S Kumar, Asit Ranjan Ghosh
Probiotics are known for their health promoting abilities through the production of various metabolites and also benefits are membrane associated. Probiotic Enterococcus faecalis AG5 has been previously assessed for its probiotic properties and this study further strengthens that claim. Strain AG5 was inoculated with 0.3% of sodium glychocholate (glycine conjugated) and Bile salt mixture at different time periods respectively. The percentage assimilation of cholesterol ranged from 14% to 33.1% in the bile salt mixture; but was slightly higher ranging from 16...
February 17, 2019: FEMS Microbiology Letters
Maria Wiese
The increasing efforts to substitute antibiotics and improve animal health combined with the acknowledgement of the role of gut microbiota in health, have led to an elevated interest in the understanding on how fibre with prebiotic potential, such as pectin, can improve animal growth and health via direct or gut microbiota mediated effects. Various reports exist on the antiviral and antibacterial effects of pectin, as well as its potency as a modulator of the immune response and gut microbial community. Comprehensive insights into the potential of pectin to improve animal growth and health are currently still hampered by heterogeneity in the design of studies...
February 15, 2019: FEMS Microbiology Letters
Verónica Perez-Cabezas, Carmen Ruiz-Molinero, Bernardo Nuñez-Moraleda, José Jesús Jimenez-Rejano, Raquel Chillón-Martinez, Jose A Moral-Munoz
Zika virus (Zikv) infection implies a significant public health issue due to the different syndromes associated. It is related to several central nervous system problems, such as microcephaly and Guillain-Barre Syndrome. In this way, the first document about the relation of Guillain-Barre Syndrome and Zika indexed in Web of Science was published in 2014. Therefore, the primary goal of the present study is to analyze the leading scientific producers, the relative specialization of the countries, and the collaboration network in the research area...
February 13, 2019: FEMS Microbiology Letters
Juanjuan Song, Shujie Zhang, Yanli Xie, Qian Li
Aflatoxin is a highly toxic mycotoxin produced by Aspergillus flavus and A.parasitic fungus. Since it contaminates food and grain widely, it has seriously endanger the health of human beings and animals. Some microorganisms that exist in nature can degrade aflatoxin. In this paper, the biological AFB1 (aflatoxin B1) -degradation by Pseudomonas aeruginosa M19 was evaluated. We found that the culture supernatant of Pseudomonas aeruginosa M19 added with proteinase K (Prok), SDS and heating significantly decreased degradation capacity...
February 11, 2019: FEMS Microbiology Letters
Dahyun Hwang, Young-Hee Lim
Resveratrol (RSV), a phytoalexin found in grapes and other plants, is known to have antibacterial effects against Escherichia coli. In this study, we aimed to identify the target gene(s) for the antibacterial activity of RSV in E. coli. Using a DNA microarray, we found that exposure to RSV led to changes in the expression levels of iron metabolism genes, and those involved in drug response and respiration. Thus, we measured the antibacterial activity of RSV against fourteen E. coli mutants with deletions in genes involved in these processes and found over fourfold higher growth inhibition in strains defective in AcrAB-TolC pump-related genes...
February 7, 2019: FEMS Microbiology Letters
Carol H Pong, Christopher J Harmer, Sandro F Ataide, Ruth M Hall
The insertion sequence IS26 plays a major role in the mobilization, expression and dissemination of antibiotic resistance genes in Gram negative bacteria. Though IS26 is abundant in sequenced genomes and in plasmids that harbour antibiotic resistance genes, only a few minor variations in the IS26 sequence have been recorded. The most common variant, IS26* (also known as IS15Δ1), encodes a Tnp26 transposase with a single amino acid substitution, G184N in the catalytic domain. Using computational modelling, this substitution was predicted to increase the length of the helix that includes the E173 residue of the catalytic DDE triad, and its effect on activity was tested...
February 7, 2019: FEMS Microbiology Letters
Mitsuhiro Itaya, Mayumi Nagasaku, Tomoe Shimada, Naoto Ohtani, Yuh Shiwa, Hirofumi Yoshikawa, Shinya Kaneko, Masaru Tomita, Mitsuru Sato
Bacillus subtilis (natto) is generally regarded as a safe bacterium and used as a host for the production of several materials. However, genetic engineering of B. subtilis (natto) is not well established because of poor DNA delivery methods and the lack of a standard strain for the aim. Here, we developed a genetic delivery tool in B. subtilis (natto) using the pLS20 conjugational plasmid (65 kbp). Transmission of pLS20 from B. subtilis 168 to wild-type B. subtilis (natto) did not occur via established mating protocols...
February 6, 2019: FEMS Microbiology Letters
Bob Dolan
No abstract text is available yet for this article.
February 1, 2019: FEMS Microbiology Letters
Dongwen Ma, Yidan Chen, Tingtao Chen
Bacterial vaginosis (BV), caused by the vaginal dysbacteriosis as well as the excessive growth of pathogenic bacteria, is a pathological condition of the vagina; its treatment using the antibiotics metronidazole or clindamycin often causes high recurrence rates. Considering the similar physiological environments of the intestinal tract and vaginal tract, as well as the pathological mechanism of intestinal infection and vaginal infection, we first propose the conception of vaginal microbiota transplantation (VMT) and discuss its potential use in BV...
January 31, 2019: FEMS Microbiology Letters
Yuru Hou, Yang Min, Hexun Jiang, Dairong Li, Yonghong Du
Low-intensity and low-frequency ultrasound (LILFU) can enhance bactericidal action of antibiotics against various sensitive bacterial species. The present study investigated to clarify the effects of low-intensity and low-frequency ultrasound (LILFU) combined with tobramycin on ESBLs E.coli biofilm (a multi-drug resistant bacteria). The biofilms of ESBLs E. coli were established and treated with ultrasound (42 kHz and ISATA of 0.66 W/cm2) continuously for 0.5 h with and without tobramycin. The bacterial viability, the morphology and the antibiotic penetration of ESBLs E...
January 31, 2019: FEMS Microbiology Letters
Mette Lübeck, Peter Stephensen Lübeck
Lactic acid bacteria (LAB) have extensive industrial applications as producers of lactic acid, as probiotics, as biocontrol agents and as biopreservatives. LAB play a large role in food fermentation and in silage processes where crops such as grass, legumes, cereals or corn are fermented into high-moisture feed that is storable and can be used to feed cattle, sheep or other ruminants. LAB also have great applications within green biorefineries, with simultaneous production of protein-rich feed for monogastric animals, silage or feed pellets for ruminants and production of lactic acid or specific amino acids...
January 30, 2019: FEMS Microbiology Letters
Luke R Gudde, Martin Hulce, Alexander H Largen, Josef D Franke
Oxidosqualene cyclases (OSCs) are remarkable enzymes that catalyze the production of the first sterol, lanosterol, in sterol biosynthetic pathways. These reactions are present in a limited number of bacterial species unlike eukaryotic species where sterol synthesis is ubiquitous. The biological role(s) of OSCs, and the sterols produced by the different sterol biosynthetic pathways in bacteria, are not clearly understood. Here, we show that inhibition of the G. obscuriglobus OSC enzyme resulted in the inability of cells to form colonies on solid medium and resulted in cell death within 24 hours of inactivation for planktonic cells...
January 30, 2019: FEMS Microbiology Letters
Thierry K S Janssens, Olaf Tyc, Harrie Besselink, Wietse de Boer, Paolina Garbeva
Pyrazines are 1,4- diazabenzene based volatile organic compounds and known for their broad-spectrum antimicrobial activity. In the present study we assessed the antimicrobial activity of 2,5-bis(1-methylethyl)-pyrazine, produced by Paenibacillus sp. AD87 during co-culture with Burkholderia sp. AD24. In addition, we were using transcriptional reporter assays in E. coli and mammalian cells to decipher the possible mode of action. Bacterial and mammalian luciferase reporter strains were deployed to elucidate antimicrobial and toxicological effects of 2,5-bis(1-methylethyl)-pyrazine...
January 29, 2019: FEMS Microbiology Letters
Michael Egermeier, Michael Sauer, Hans Marx
The yeast Yarrowia lipolytica represents a future microbial cell factory for numerous applications in a bio-based economy. Outstanding feature of this yeast is the metabolic flexibility in utilizing various substrates (sugars, fatty acids, glycerol, etc.). The potential of wild-type isolates of Y. lipolytica to convert glycerol into various value-added compounds is attracting attention of academia and industry. However, the already established tools for efficient engineering of the metabolism of Y. lipolytica are often dependent on genetic features like auxotrophic markers...
January 29, 2019: FEMS Microbiology Letters
A H Bishop
The microorganisms with which we share our world go largely un-noticed. We are, however, beginning to be able to exploit their apparently silent presence as witnesses to events that are of legal concern. This information can be used to link forensic samples to criminal events and even perpetrators. Once dead, our bodies are rapidly colonised, internally and externally. The progress of these events can be charted to inform how long and even by what means a person has died. A small number of microbial species could actually be the cause of such deaths as a result of biocrime or bioterrorism...
January 28, 2019: FEMS Microbiology Letters
Olouwafemi Mistourath Mama, Laura Ruiz-Ripa, Rosa Fernández-Fernández, David González-Barrio, Jose Francisco Ruiz-Fons, Carmen Torres
The objective was to determine the frequency and the diversity of coagulase-positive staphylococci (CoPS) in nasal samples of healthy wild boar, to study their resistance phenotypes/genotypes and to check the occurrence of the MRSA-ST398. Nasal samples of 371 wild boars were collected in Spain for staphylococci and MRSA recovery. Staphylococci identification was performed by MALDI-TOF. The susceptibility to eleven antimicrobials was tested by disc-diffusion and the presence of resistance genes by PCR. Molecular typing and virulence factors determination were carried out by PCR and sequencing...
January 25, 2019: FEMS Microbiology Letters
Zhi-Ming Wu, Yong Yang, Kun-Tai Li
Antifungalmycin N2 (3-methyl-3,5-amino-4-vinyl-2-pyrone, C6H7O2N) is a novel bioactive substance produced by Streptomyces sp. N2. In the present work, the antagonistic activity of antifungalmycin N2 and its biocontrol efficacy on Rhizoctonia solani were carried out to evaluate its potential as a biocontrol agent against fungal plant diseases. By using potato dextrose agar (PDA) media for in vitro cultivation of phytopathogenic fungi, the results showed that antifungalmycin N2 not only displayed broad-spectrum antifungal activities against various plant pathogenic fungi, but also had a strong antagonism to the sclerotial germination of R...
January 23, 2019: FEMS Microbiology Letters
Thi Thuy Do, Sarah Delaney, Fiona Walsh
There is little known about the wastewater treatment plant (WWTP) effluent microbiome, its structure and dynamics. Here we provide a study of the microbiome of effluent leaving conventional WWTPs and entering the water environment. DNA was extracted from WWTP effluent samples collected in 2015 and 2016. The bacterial communities were studied using Illumina MiSeq 16S rRNA gene amplicon sequencing and analysed using Calypso software. The Proteobacteria, Bacteroides, Actinobacteria, Firmicutes, Tenericutes and Verrucomicrobia phyla dominated the microbiomes...
January 23, 2019: FEMS Microbiology Letters
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