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Veterinary Microbiology

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https://read.qxmd.com/read/30683303/corrigendum-to-beta-propiolactone-inactivated-bivalent-bluetongue-virus-vaccine-containing-montanide-isa-71vg-adjuvant-induces-long-term-immune-response-in-sheep-against-serotypes-4-and-16-even-after-3-years-of-controlled-vaccine-storage-vet-microbiol-226-2018
#1
Kuandyk Zhugunissov, Yerbol Bulatov, Dmitriy Taranov, Zakir Yershebulov, Zhumagali Koshemetov, Asankadyr Zhunushov, Gourapura J Renukaradhya, Kaissar Tabynov, Yergali Abduraimov
No abstract text is available yet for this article.
January 22, 2019: Veterinary Microbiology
https://read.qxmd.com/read/30658866/syncytia-generated-by-hemagglutinin-neuraminidase-and-fusion-proteins-of-virulent-newcastle-disease-virus-induce-complete-autophagy-by-activating-ampk-mtorc1-ulk1-signaling
#2
Shanhui Ren, Zaib Ur Rehman, Mengyu Shi, Bin Yang, Yurong Qu, Xiao Feng Yang, Qi Shao, Chunchun Meng, Zengqi Yang, Xiaolong Gao, Yingjie Sun, Chan Ding
Autophagy triggered by glycoprotein-mediated membrane fusion has been reported for several paramyxoviruses. However, the function of HN and F glycoproteins of NDV and their role in autophagy induction have not been studied. Here, we found that co-transfection of HN and F of virulent NDV rapidly induced syncytium formation and triggered a steady state autophagy flux in adenocarcinomic human alveolar basal epithelial (A549) cells and chicken embryo fibroblast (DF-1) cells. Furthermore, we clearly identified that F and HN synergistically induced autophagosome fusion with lysosomes for subsequent degradation...
January 4, 2019: Veterinary Microbiology
https://read.qxmd.com/read/30573226/corrigendum-to-p53-mediated-ifn-%C3%AE-signaling-to-affect-viral-replication-upon-tgev-infection-vet-microbiol-227-2018-61-68
#3
Li Ding, Jiawei Li, Weihao Li, Zhenhua Fang, Na Li, Qiqi Guo, Haoyue Qu, Dan Feng, Jiangyue Li, Meiling Hong
No abstract text is available yet for this article.
December 17, 2018: Veterinary Microbiology
https://read.qxmd.com/read/30567631/a-reply-to-a-comment-on-factors-influencing-the-outcome-of-primary-immunization-against-rabies-in-young-dogs
#4
LETTER
Konstantia E Tasioudi
No abstract text is available yet for this article.
December 13, 2018: Veterinary Microbiology
https://read.qxmd.com/read/30545668/corrigendum-to-prophage-phiv142-3-enhances-the-colonization-and-resistance-to-environmental-stresses-of-avian-pathogenic-escherichia-coli-vet-microbiol-218-2018-70-77
#5
Dezhi Li, Fang Tang, Feng Xue, Jianluan Ren, Yun Liu, Dehong Yang, Jianjun Dai
No abstract text is available yet for this article.
December 10, 2018: Veterinary Microbiology
https://read.qxmd.com/read/30497811/corrigendum-to-multilocus-sequence-typing-of-mycoplasma-bovis-reveals-host-specific-genotypes-in-cattle-versus-bison-vet-microbiol-175-1-2015-92-98
#6
Karen B Register, Luke Thole, Ricardo F Rosenbush, F Chris Minion
No abstract text is available yet for this article.
November 26, 2018: Veterinary Microbiology
https://read.qxmd.com/read/30471786/corrigendum-to-a-large-scale-serological-survey-of-akabane-virus-infection-in-cattle-yak-sheep-and-goats-in-china-vet-microbiol-207-2017-7-12
#7
Jidong Wang, Kim R Blasdell, Hong Yin, Peter J Walker
No abstract text is available yet for this article.
November 21, 2018: Veterinary Microbiology
https://read.qxmd.com/read/30642603/the-bacterial-and-fungal-microbiome-of-the-skin-of-healthy-dogs-and-dogs-with-atopic-dermatitis-and-the-impact-of-topical-antimicrobial-therapy-an-exploratory-study
#8
Suttiwee Chermprapai, Thomas H A Ederveen, Femke Broere, Els M Broens, Yvette M Schlotter, Saskia van Schalkwijk, Jos Boekhorst, Sacha A F T van Hijum, Victor P M G Rutten
Canine atopic dermatitis is a genetically predisposed inflammatory and pruritic allergic skin disease that is often complicated by (secondary) bacterial and fungal (yeast) infections. High-throughput DNA sequencing was used to characterize the composition of the microbiome (bacteria and fungi) inhabiting specific sites of skin in healthy dogs and dogs with atopic dermatitis (AD) before and after topical antimicrobial treatment. Skin microbiome samples were collected from six healthy control dogs and three dogs spontaneously affected by AD by swabbing at (non-) predilection sites before, during and after treatment...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642602/mutations-associated-with-change-of-susceptibility-to-lincosamides-and-or-macrolides-in-field-and-laboratory-derived-mycoplasma-californicum-strains-in-japan-and-development-of-a-rapid-detection-method-for-these-mutations
#9
Eiji Hata, Kazuya Nagai, Kenji Murakami
Five mutations involved in changing of susceptibility to lincosamides and/or macrolides were investigated in field isolates of Mycoplasma californicum in Japan, and reconfirmed in laboratory-derived mutants. In addition, a quick and easy detection method for these mutations was established. Guanine at position 748 (Escherichia coli numbering) of the 23S rRNA gene (rrl) was shown to be involved with decreased susceptibility to 16-membered macrolides, and adenines at positions 2059 and 2062 of rrl were involved with decreased susceptibility to both lincosamides and macrolides...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642601/preventive-effect-of-the-phage-vb-savm-jyl01-on-rabbit-necrotizing-pneumonia-caused-by-staphylococcus-aureus
#10
Yalu Ji, Mengjun Cheng, Shengjie Zhai, Hengyu Xi, Ruopeng Cai, Zijing Wang, Hao Zhang, Xinwu Wang, Yibing Xue, Xinwei Li, Changjiang Sun, Xin Feng, Liancheng Lei, Sadeeq Ur Rahman, Wenyu Han, Jingmin Gu
Staphylococcus aureus is one of the most important pathogens causing rabbit necrotizing pneumonia and brings huge economic losses to rabbit production. This study investigated the preventive effect of a phage on rabbit necrotizing pneumonia caused by S. aureus. S. aureus S6 was isolated from the lungs of rabbits suffering necrotizing pneumonia and identified. A novel phage named VB-SavM-JYL01 was isolated by using S. aureus S6 as a host and showed a broader host range than the phages GH15 and K. The genome of VB-SavM-JYL01 lacked bacterial virulence-, antibiotic resistance- and lysogenesis-related genes...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642600/comparison-of-vaccination-protocols-against-mycoplasma-hyopneumoniae-during-the-gilt-acclimation-period
#11
Laura Garza-Moreno, Maria Pieters, Sergio López-Soria, Marta Carmona, Roman Krejci, Joaquim Segalés, Marina Sibila
This study evaluated different gilt vaccination protocols against Mycoplasma (M.) hyopneumoniae at acclimation and their effect on the genetic diversity. A total of 180 M. hyopneumoniae naïve gilts were selected 1 week post-entry (wpe) at the acclimation barn in a clinically affected M. hyopneumoniae farm. Gilts were distributed according to the M. hyopneumoniae antibodies levels into three different vaccination schedules: A) four doses of a M. hyopneumoniae commercial vaccine at 2, 4, 6 and 8 wpe; B) two vaccine doses at 2 and 6 wpe and PBS at 4 and 8 wpe; and C) four PBS doses at the same wpe...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642599/antimicrobial-resistance-in-brachyspira-an-increasing-problem-for-disease-control
#12
REVIEW
David J Hampson, Kittitat Lugsomya, Tom La, Nyree Dale Phillips, Darren J Trott, Sam Abraham
Across all bacterial species the continuing reduction in susceptibility to antimicrobial agents is a critical and increasing threat for disease control. This mini-review outlines the extent of this problem amongst anaerobic intestinal spirochaetes of the genus Brachyspira, of which there are currently nine officially recognised species. These include some important pathogens that may cause colitis with diarrhoea and/or dysentery in various mammalian and avian species, but most notably in pigs and in adult chickens...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642598/use-of-a-modified-bacterial-ghost-lysis-system-for-the-construction-of-an-inactivated-avian-pathogenic-escherichia-coli-vaccine-candidate
#13
Jiangang Hu, Jiakun Zuo, Zhaoguo Chen, Lixia Fu, Xiaolong Lv, Shijun Hu, Xingchi Shi, Yawei Jing, Yalei Wang, Zhihao Wang, Rongsheng Mi, Yan Huang, Dahai Liu, Kezong Qi, Xiangan Han
Vaccination is an effective strategy to prevent avian colibacillosis. Bacterial ghosts (BGs) are prepared by the controlled expression of the phiX174 gene E, which mediates the lysis of Gram-negative bacteria. Staphylococcal nuclease A may be used to produce BGs for further inactivation of host bacteria and elimination of residual genetic material. In this study, the double promoter lysis plasmid (pUC19-ΔcI857-E-rrnB-pL-SN) was successfully constructed and BGs were prepared at 37 °C. The cleavage efficiency of Escherichia coli BGs was 99...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642597/newcastle-disease-virus-like-particles-containing-the-brucella-bcsp31-protein-induce-dendritic-cell-activation-and-protect-mice-against-virulent-brucella-challenge
#14
Xiaohong Xu, Zhuang Ding, Jindou Li, Jiaming Liang, Zhaoyang Bu, Jiaxin Ding, Yanling Yang, Xulong Lang, Xinglong Wang, Renfu Yin, Jing Qian
Brucellosis is a widespread zoonosis that poses a substantial threat to human and animal public health due to the absence of a sufficiently safe and efficient vaccine. Virus-like particles (VLPs) have been developed as novel vaccine candidates and suitable carrier platforms for the delivery of exogenous proteins. Herein, we constructed chimeric virus-like particles (cVLPs) assembled by a Newcastle disease virus (NDV) M protein and glycosylphosphatidylinositol-anchored Brucella BCSP31 protein (GPI-BCSP31). cVLPs-GPI-BCSP31 were highly efficient in murine dendritic cell (DC) activation, both in vitro and in vivo...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642596/porcine-reproductive-and-respiratory-syndrome-virus-counteracts-type-i-interferon-induced-early-antiviral-state-by-interfering-irf7-activity
#15
Ke Liu, Gaini Ma, Xiqian Liu, Yan Lu, Shumin Xi, Anni Ou, Jianchao Wei, Beibei Li, Donghua Shao, Yuming Li, Yafeng Qiu, Denian Miao, Zhiyong Ma
Porcine reproductive and respiratory syndrome (PRRS) is an economically important disease with a significant impact on the pig industry. It is caused by PRRS virus (PRRSV), which predominantly infects and replicates in porcine pulmonary alveolar macrophages (PAMs). We pretreated PAMs with porcine interferon (IFN)-α to induce an antiviral state within the cells and subsequently infected them with highly pathogenic PRRSV. Changes in global gene expression in IFN-α-pretreated PAMs in response to PRRSV infection were determined by RNA-sequence analysis and confirmed by real-time PCR...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642595/detection-of-infectious-bursal-disease-virus-ibdv-antibodies-using-chimeric-plant-virus-like-particles
#16
Kadagala Divya Sahithi, Pandirajan Arul Nancy, Garla Prabhakar Vishnu Vardhan, Kathaperumal Kumanan, Kanagaraj Vijayarani, Masarapu Hema
The aim of the present study is to use Physalis mottle virus (PhMV) coat protein (CP) as a scaffold to display the neutralizing epitopes of Infectious bursal disease virus (IBDV) VP2. For this, three different chimeric constructs were synthesized by replacing the N-terminus of PhMV CP with tandem repeats of neutralizing epitopes of IBDV VP2 and expressed in Escherichia coli. Expression analysis revealed that all the three recombinant chimeric coat protein subunits are soluble in nature and self-assembled into virus-like particles (VLPs) as evidenced through sucrose density gradient ultracentrifugation...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642594/experimental-infection-of-rabbit-with-swine-derived-hepatitis-e-virus-genotype-4
#17
Baoyuan Liu, Yiyang Chen, Yani Sun, Yuchen Nan, Huixia Li, Taofeng Du, Julian A Hiscox, Qin Zhao, En-Min Zhou
Hepatitis E virus (HEV) is a zoonotic virus that is capable of causing cross-species infection. Rabbits can be experimentally infected with human- and swine-derived HEV-4 in the species Orthohepevirus A, and avian-derived HEV-3 in the species Orthohepevirus B suggesting rabbits can serve as an animal model for zoonotic HEV infection study. However, these studies show that the infectivity of swine HEV isolates in rabbits is not consistent. In this study, the animal study was conducted by the experimental infection of rabbit with a swine-derived HEV-4 isolated in China (designated CHN-SD-sHEV) for 28 weeks post-inoculation (wpi) in compassion to that infected with a rabbit-derived HEV-3 (designated HEV-SX-rHEV)...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642593/lactobacillus-acidophilus-s-layer-protein-mediated-inhibition-of-pedv-induced-apoptosis-of-vero-cells
#18
Xiaoyan Zhang, Pengcheng Li, Qisheng Zheng, Jibo Hou
To gain insight into the mechanism of Lactobacillus acidophilus (L. acidophilus) S-layer protein antiviral activity, we examined how S-layer protein impacts porcine epidemic diarrhea virus (PEDV) infection and PEDV-induced apoptosis of Vero cells. Pretreatment (exclusion assay), coincubation (competition assay), and post-treatment (displacement assay) of PEDV-infected Vero cells with the S-layer protein was examined. Interestingly, significant inhibition of PEDV by S-layer protein was only observed in the exclusion assay...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642592/bovine-herpesvirus-5-promotes-mitochondrial-dysfunction-in-cultured-bovine-monocyte-derived-macrophages-and-not-affect-virus-replication
#19
Tereza C Cardoso, Lucas H Okamura, Jamila C Baptistella, Ana Carolina Borsanelli, Lillian Baptistiolli, Helena L Ferreira, Roberto Gameiro, Eduardo F Flores
Bovine alphaherpesvirus 1 (BHV1) and 5 (BHV5) are known to establish latent infections in sensory neurons of the trigeminal ganglion, yet leukocytes and tonsils have also been described as sites of latency in experimentally infected cattle. Little information is available on which immune cells are susceptible to BHV5 infection and how the infection may affect cell bioenergy. The aim of this study was to determine whether primary bovine monocyte-derived macrophages are susceptible to BHV5 infection and to evaluate parameters such as cell survival, virus replication and nitric oxide (NO) production...
February 2019: Veterinary Microbiology
https://read.qxmd.com/read/30642591/expression-of-the-feline-leukemia-virus-subgroup-c-receptors-in-normal-and-neoplastic-urothelium-of-the-urinary-bladder-of-cattle-associated-with-bovine-papillomavirus-infection
#20
Valeria Russo, Franco Roperto, Marian Taulescu, Francesca De Falco, Chiara Urraro, Federica Corrado, John S Munday, Cornel Catoi, Sante Roperto
The feline leukemia virus subgroup C receptors (FLVCRs) were originally cloned as virus receptors, but are now believed to function also as heme transporters and are expressed in a broad range of tissues in a wide range of mammalian species. Expression of FLVCR1 and FLVCR2 was investigated in 19 bovine papillomavirus-associated urinary bladder cancers and in 15 non-neoplastic samples of bladder from cattle. E5 oncoprotein of bovine Deltapapillomaviruses (δPVs) was detected in 17 of the 19 bladder cancers. Flvcr1 and Flvcr2 were amplified and sequenced both in neoplastic and non-neoplastic samples showing a 100% identity with bovine Flvcr1 and Flvcr2 mRNA sequences present in GenBank database (accession numbers: NM_001206019...
February 2019: Veterinary Microbiology
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