journal
Journals Investigative Ophthalmology & ...

Investigative Ophthalmology & Visual Science

https://read.qxmd.com/read/36951855/a-duration-dependent-interaction-between-high-intensity-light-and-unrestricted-vision-in-the-drive-for-myopia-control
#1
JOURNAL ARTICLE
Sayantan Biswas, Arumugam R Muralidharan, Bjorn Kaijun Betzler, Joanna Marie Fianza Busoy, Veluchamy A Barathi, Royston K Y Tan, Wan Yu Shermaine Low, Dan Milea, Biten K Kathrani, Noel A Brennan, Raymond P Najjar
PURPOSE: To evaluate the duration-dependent and synergetic impact of high-intensity light (HL) and unrestricted vision (UnV) on lens-induced myopia (LIM) development in chickens. METHODS: Myopia was induced in one eye in chicks (10 groups, n = 126) from day 1 posthatching (D1) until day 8 (D8) using -10 diopter (D) lenses. Fellow eyes remained uncovered as controls. Nine groups were exposed daily to 2, 4, or 6 hours of HL (15,000 lux), UnV (removal of -10 D lens), or both (HL + UnV)...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36943152/integrative-single-cell-rna-seq-and-atac-seq-analysis-of-mouse-corneal-epithelial-cells
#2
JOURNAL ARTICLE
Zhao-Jing Lu, Jin-Guo Ye, Dong-Liang Wang, Meng-Ke Li, Qi-Kai Zhang, Zhong Liu, Yan-Jing Huang, Cai-Neng Pan, Yu-Heng Lin, Zhuo-Xing Shi, Ying-Feng Zheng
PURPOSE: Corneal epithelial homeostasis is maintained by coordinated gene expression across distinct cell populations, but the gene regulatory programs underlying this cellular diversity remain to be characterized. Here we applied single-cell multi-omics analysis to delineate the gene regulatory profile of mouse corneal epithelial cells under normal homeostasis. METHODS: Single cells isolated from the cornea epithelium (with marginal conjunctiva) of adult mice were subjected to scRNA-seq and scATAC-seq using the 10×Genomics platform...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36939720/vitreous-fatty-amides-and-acyl-carnitines-are-altered-in-intermediate-age-related-macular-degeneration
#3
JOURNAL ARTICLE
Chang-Ki Yoon, Ye An Kim, Un Chul Park, Seung-Hyun Kwon, Young Lee, Hyun Ju Yoo, Je Hyun Seo, Hyeong Gon Yu
PURPOSE: Age-related macular degeneration (AMD) is the leading cause of visual impairment worldwide. In this study, we aimed to investigate the vitreous humor metabolite profiles of patients with intermediate AMD using untargeted metabolomics. METHODS: We performed metabolomics using high-resolution liquid chromatography mass spectrometry on the vitreous humor of 31 patients with intermediate AMD and 30 controls who underwent vitrectomy for epiretinal membrane with or without cataract surgery...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36939719/differences-in-outflow-facility-between-angiographically-identified-high-versus-low-flow-regions-of-the-conventional-outflow-pathways-in-porcine-eyes
#4
JOURNAL ARTICLE
Clemens A Strohmaier, Fiona S McDonnell, Xiaowei Zhang, Daniel Wanderer, W Daniel Stamer, Robert N Weinreb, Alex S Huang
PURPOSE: To investigate differences in outflow facility between angiographically determined high- and low-flow segments of the conventional outflow pathway in porcine eyes. METHODS: Porcine anterior segments (n = 14) were mounted in a perfusion chamber and perfused using Dulbecco's phosphate buffered solution with glucose. Fluorescein angiography was performed to determine high- and low-flow regions of the conventional outflow pathways. The trabecular meshwork (TM) was occluded using cyanoacrylate glue, except for residual 5-mm TM areas that were either high or low flow at baseline, designating these eyes as "residual high-flow" or "residual low-flow" eyes...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36930145/scleral-proteome-in-noninfectious-scleritis-unravels-upregulation-of-filaggrin-2-and-signs-of-neovascularization
#5
JOURNAL ARTICLE
Daphne P C Vergouwen, Josianne C Ten Berge, Coskun Guzel, Thierry P P van den Bosch, Robert M Verdijk, Aniki Rothova, Theo M Luider, Marco W J Schreurs
PURPOSE: Scleritis is a severe inflammatory ocular disorder with unknown pathogenesis. We investigated healthy sclera as well as sclera affected by noninfectious scleritis for differentially expressed proteins using a mass spectrometry approach. METHODS: We collected scleral samples of enucleated eyes due to severe noninfectious scleritis (n = 3), and control scleral tissues (n = 5), all exenterated eyes for eyelid carcinomas (n = 4), or choroidal melanoma (n = 1) without scleral invasion...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36920364/aim2-deficiency-ameliorates-lacrimal-gland-destruction-and-corneal-epithelium-defects-in-an-experimental-dry-eye-model
#6
JOURNAL ARTICLE
Yu Chen, Jiheng Pu, Xinda Li, Lili Lian, Chaoxiang Ge, Zuimeng Liu, Weizhuo Wang, Ling Hou, Wei Chen, Jinyang Li
PURPOSE: Dry eye disease (DED) is a multifactorial disease that is associated with inflammation. Excessive DNA is present in the tear fluid of patients with DED. Absent in melanoma 2 (AIM2) is a key DNA sensor. This study aimed to investigate the role of AIM2 in the pathogenesis of DED. METHODS: DED was induced by injection of scopolamine (SCOP). Aberrant DNA was detected by cell-free DNA (cfDNA) ELISA and immunostaining. Corneal epithelial defects were assessed by corneal fluorescein staining, zonula occludens-1 immunostaining and TUNEL...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36917121/the-genetic-confirmation-and-clinical-characterization-of-loxl3-associated-myp28-a-common-type-of-recessive-extreme-high-myopia
#7
JOURNAL ARTICLE
Yi Jiang, Lin Zhou, Yingwei Wang, Jiamin Ouyang, Shiqiang Li, Xueshan Xiao, Xiaoyun Jia, Junwen Wang, Zhen Yi, Wenmin Sun, Xiaodong Jiao, Panfeng Wang, J Fielding Hejtmancik, Qingjiong Zhang
PURPOSE: In previous studies, biallelic LOXL3 variants have been shown to cause autosomal recessive Stickler syndrome in one Saudi Arabian family or autosomal recessive early-onset high myopia (eoHM, MYP28) in two Chinese families. The current study aims to elucidate the clinical and genetic features of LOXL3-associated MYP28 in seven new families and two previously published families. METHODS: LOXL3 variants were detected based on the exome sequencing data of 8389 unrelated probands with various ocular conditions...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36912597/ferritin-but-not-iron-increases-in-retina-upon-systemic-iron-overload-in-diabetic-and-iron-dextran-injected-mice
#8
JOURNAL ARTICLE
Aina Bonet, Judit Pampalona, Eduard Jose-Cunilleras, Víctor Nacher, Jesús Ruberte
PURPOSE: Iron overload causes oxidative damage in the retina, and it has been involved in the pathogeny of diabetic retinopathy, which is one of the leading causes of blindness in the adult population worldwide. However, how systemic iron enters the retina during diabetes and the role of blood retinal barrier (BRB) in this process remains unclear. METHODS: The db/db mouse, a well-known model of type 2 diabetes, and a model of systemic iron overload induced by iron dextran intraperitoneal injection, were used...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36912596/phenotypic-variability-of-retinal-disease-among-a-cohort-of-patients-with-variants-in-the-cln-genes
#9
JOURNAL ARTICLE
Masha Kolesnikova, Jose Ronaldo Lima de Carvalho, Jin Kyun Oh, Megan Soucy, Aykut Demirkol, Angela H Kim, Stephen H Tsang, Mark P Breazzano
PURPOSE: To describe the phenotype of CLN-associated retinal dystrophy in a subset of patients at the Columbia University Medical Center, United States, and the Hospital das Clínicas de Pernambuco, Brazil, in comparison to the published literature. METHODS: Eleven patients with confirmed biallelic variants in the CLN genes were evaluated via dilated fundus examination, clinical imaging, and full-field electroretinogram. A thorough literature search was conducted to determine previously published variants and associated phenotypes...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36897151/overactivation-of-norepinephrine-%C3%AE-2-adrenergic-receptor-axis-promotes-corneal-neovascularization
#10
JOURNAL ARTICLE
Qiaoqiao Dong, Benxiang Qi, Bin Zhang, Xiaoyun Zhuang, Shijiu Chen, Qingjun Zhou, Bi Ning Zhang, Suxia Li
PURPOSE: To investigate the role of the sympathetic nervous system in corneal neovascularization (CNV) and to identify the downstream pathway involved in this regulation. METHODS: Three types of CNV models were constructed with C57BL/6J mice, including the alkali burn model, suture model, and basic fibroblast growth factor (bFGF) corneal micropocket model. Subconjunctival injection of the sympathetic neurotransmitter norepinephrine (NE) was administered in these three models...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36897150/choroidal-microvasculature-dropout-in-the-absence-of-parapapillary-atrophy-in-poag
#11
JOURNAL ARTICLE
Eun Ji Lee, Ji Eun Song, Hye Seong Hwang, Jeong-Ah Kim, Seung Hyen Lee, Tae-Woo Kim
PURPOSE: To describe the parapapillary choroidal microvasculature dropout (CMvD) in glaucomatous eyes without β-zone parapapillary atrophy (β-PPA) and compare its characteristics with that of CMvD with β-PPA. METHODS: Peripapillary choroidal microvasculature was evaluated on en face images obtained using optical coherence tomography angiography. CMvD was defined as a focal sectoral capillary dropout with no visible microvascular network identified in the choroidal layer...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36892534/tissue-specific-sex-difference-in-mouse-eye-and-brain-metabolome-under-fed-and-fasted-states
#12
JOURNAL ARTICLE
Meghashri Saravanan, Rong Xu, Olivia Roby, Yekai Wang, Siyan Zhu, Amy Lu, Jianhai Du
PURPOSE: Visual physiology and various ocular diseases demonstrate sexual dimorphisms; however, how sex influences metabolism in different eye tissues remains undetermined. This study aims to address common and tissue-specific sex differences in metabolism in the retina, RPE, lens, and brain under fed and fasted conditions. METHODS: After ad libitum fed or being deprived of food for 18 hours, mouse eye tissues (retina, RPE/choroid, and lens), brain, and plasma were harvested for targeted metabolomics...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36892533/missense-mutations-in-mab21l1-causation-of-novel-autosomal-dominant-ocular-bamd-syndrome
#13
JOURNAL ARTICLE
Panfeng Wang, Pengsen Wu, Junwen Wang, Yiyan Zeng, Yi Jiang, Yingwei Wang, Shiqiang Li, Xueshan Xiao, Qingjiong Zhang
PURPOSE: Biallelic MAB21L1 variants have been reported to cause autosomal recessive cerebellar, ocular, craniofacial, and genital syndrome (COFG), whereas only five heterozygous pathogenic variants have been suspected to cause autosomal dominant (AD) microphthalmia and aniridia in eight families. This study aimed to report an AD ocular syndrome (blepharophimosis plus anterior segment and macular dysgenesis [BAMD]) syndrome based on clinical and genetic findings from patients with monoallelic MAB21L1 pathogenic variants in our cohort and reported cases...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36881408/sirt1-inhibits-high-glucose-induced-txnip-nlrp3-inflammasome-activation-and-cataract-formation
#14
JOURNAL ARTICLE
Lili Lian, Zhenmin Le, Zhenzhen Wang, Ying-Ao Chen, Xiaodong Jiao, Hang Qi, J Fielding Hejtmancik, Xiaoyin Ma, Qinxiang Zheng, Yueping Ren
PURPOSE: To determine whether SIRT1 regulates high glucose (HG)-induced inflammation and cataract formation through modulating TXNIP/NLRP3 inflammasome activation in human lens epithelial cells (HLECs) and rat lenses. METHODS: HG stress from 25 to 150 mM was imposed on HLECs, with treatments using small interfering RNAs (siRNAs) targeting NLRP3, TXNIP, and SIRT1, as well as a lentiviral vector (LV) for SIRT1. Rat lenses were cultivated with HG media, with or without the addition of NLRP3 inhibitor MCC950 or SIRT1 agonist SRT1720...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36881407/spatial-resolution-evaluation-based-on-experienced-visual-categories-with-sweep-evoked-periodic-eeg-activity
#15
JOURNAL ARTICLE
Coralie Hemptinne, Nathan Hupin, Aliette Lochy, Demet Yüksel, Bruno Rossion
PURPOSE: Visual function is typically evaluated in clinical settings with visual acuity (VA), a test requiring to behaviorally match or name optotypes such as tumbling E or Snellen letters. The ability to recognize these symbols has little in common with the automatic and rapid visual recognition of socially important stimuli in real life. Here we use sweep visual evoked potentials to assess spatial resolution objectively based on the recognition of human faces and written words. METHODS: To this end, we tested unfamiliar face individuation1 and visual word recognition2 in 15 normally sighted adult volunteers with a 68-electrode electroencephalogram system...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36877516/correlation-between-retinal-vessel-diameters-and-uveitis-activity
#16
JOURNAL ARTICLE
Federico Zicarelli, Aniruddha Agarwal, Chiara Rizzi, Marta Oldani, Giovanni Staurenghi, Alessandro Invernizzi
PURPOSE: To assess retinal vein and artery diameters during active and inactive intraocular inflammation in eyes with uveitis. METHODS: Color fundus photographs and clinical data of eyes with uveitis collected during two visits (active disease [i.e., T0] and inactive stage [i.e., T1]) were reviewed. The images were semi-automatically analyzed to obtain the central retina vein equivalent (CRVE) and central retina artery equivalent (CRAE). Changes of CRVE and CRAE from T0 to T1 were calculated, and their possible correlation with clinical data, including age, gender, ethnicity, uveitis etiology, and visual acuity, were investigated...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36877515/t-cell-repertoire-analysis-in-the-conjunctiva-of-murine-dry-eye-model
#17
JOURNAL ARTICLE
Xiaorui Bao, Yanlin Zhong, Chunyan Yang, Yujie Chen, Yi Han, Xiang Lin, Caihong Huang, Kejia Wang, Zuguo Liu, Cheng Li
PURPOSE: Dry eye is closely related to the activation and proliferation of immune cells, especially T cells. However, the determination of the preferential T-cell clonotypes is technically challenging. This study aimed to investigate the characterization of T-cell receptor (TCR) repertoire in the conjunctiva during dry eye. METHODS: A desiccating stress animal model was established using C57/BL6 mice (8-10 weeks, female). After 7 days of stress stimulation, the slit-lamp image and Oregon-green-dextran staining were used to evaluate the ocular surface injury...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36877514/proposed-mechanism-of-long-term-intraocular-pressure-lowering-with-the-bimatoprost-implant
#18
JOURNAL ARTICLE
W Daniel Stamer, Kristin M Perkumas, Min H Kang, Mohammed Dibas, Michael R Robinson, Douglas J Rhee
PURPOSE: The purpose of this study was to evaluate the effects of pharmacologically relevant bimatoprost and bimatoprost free acid (BFA) concentrations on matrix metalloproteinase (MMP) gene expression in cells from human aqueous outflow tissues. METHODS: MMP gene expression by human trabecular meshwork (TM), scleral fibroblast (SF), and ciliary muscle (CM) cells exposed to 10 to 1000 µM bimatoprost or 0.1 to 10 µM BFA (intraocular concentrations after intracameral bimatoprost implant and topical bimatoprost dosing, respectively) was measured by polymerase chain reaction array...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36867134/the-role-of-c-x-c-chemokines-in-staphylococcus-aureus-endophthalmitis
#19
JOURNAL ARTICLE
Phillip S Coburn, Aaron C Parrott, Frederick C Miller, Austin L LaGrow, Md Huzzatul Mursalin, Michelle C Callegan
PURPOSE: To test the hypothesis that the C-X-C chemokines CXCL1, CXCL2, and CXCL10 contribute to inflammation during Staphylococcus aureus endophthalmitis. METHODS: S. aureus endophthalmitis was induced by intravitreal injection of 5000 colony forming units of S. aureus into the eyes of C57BL/6J, CXCL1-/-, CXCL2-/-, or CXCL10-/- mice. At 12, 24, and 36 hours postinfection, bacterial counts, intraocular inflammation, and retinal function were assessed. Based on these results, the effectiveness of intravitreal administration of anti-CXCL1 in reducing inflammation and improving retinal function was evaluated in S...
March 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/36867133/physical-activity-is-associated-with-macular-thickness-a-multi-cohort-observational-study
#20
JOURNAL ARTICLE
Ella C Berry, Henry N Marshall, Sean Mullany, Santiago Diaz Torres, Joshua Schmidt, Daniel Thomson, Lachlan S W Knight, Georgina L Hollitt, Ayub Qassim, Bronwyn Ridge, Angela Schulz, Mark M Hassall, Thi Thi Nguyen, Stewart Lake, Richard A Mills, Ashish Agar, Anna Galanopoulos, John Landers, Paul R Healey, Stuart L Graham, Alex W Hewitt, Stuart MacGregor, Robert J Casson, Owen M Siggs, Jamie E Craig
PURPOSE: To assess the association between physical activity and spectral-domain optical coherence tomography (SD-OCT)-measured rates of macular thinning in an adult population with primary open-angle glaucoma. METHODS: The correlation between accelerometer-measured physical activity and rates of macular ganglion cell-inner plexiform layer (GCIPL) thinning was measured in 735 eyes from 388 participants of the Progression Risk of Glaucoma: RElevant SNPs with Significant Association (PROGRESSA) study...
March 1, 2023: Investigative Ophthalmology & Visual Science
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