Brandi L Williams, Moussa A Zouache, Nathan A Seager, Chris M Pappas, Jin Liu, Robert A Anstadt, William C Hubbard, Julie Thomas, Jill L Hageman, Jennifer Mohler, Burt T Richards, Gregory S Hageman
PURPOSE: The purpose of this study was to determine if levels of the HtrA1 protein in serum or vitreous humor are influenced by genetic risk for age-related macular degeneration (AMD) at the 10q26 locus, age, sex, AMD status, and/or AMD disease severity, and, therefore, to determine the contribution of systemic and ocular HtrA1 to the AMD disease process. METHODS: A custom-made sandwich ELISA assay (SCTM ELISA) for detection of the HtrA1 protein was designed and compared with three commercial assays (R&D Systems, MyBiosource 1 and MyBiosource 2) using 65 serum samples...
April 1, 2024: Investigative Ophthalmology & Visual Science