journal
Journals Investigative Ophthalmology & ...

Investigative Ophthalmology & Visual Science

https://read.qxmd.com/read/37773501/paraventricular-hypothalamic-nucleus-upregulates-intraocular-pressure-via-glutamatergic-neurons
#1
JOURNAL ARTICLE
Lin Ma, Qing Liu, Xin Liu, Heng Chang, Sen Jin, Wenyu Ma, Fuqiang Xu, Haixia Liu
PURPOSE: The neuroregulatory center of intraocular pressure (IOP) is located in the hypothalamus. An efferent neural pathway exists between the hypothalamic nuclei and the autonomic nerve endings in the anterior chamber of the eye. This study was designed to investigate whether the paraventricular hypothalamic nucleus (PVH) regulates IOP as the other nuclei do. METHODS: Optogenetic manipulation of PVH neurons was used in this study. Light stimulation was applied via an optical fiber embedded over the PVH to activate projection neurons after AAV2/9-CaMKIIα-hChR2-mCherry was injected into the right PVH of C57BL/6J mice...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37773500/choroidal-changes-in-rhesus-macaques-in-aging-and-age-related-drusen
#2
JOURNAL ARTICLE
Yevgeniy Sazhnyev, Tzu-Ni Sin, Anthony Ma, Ellie Chang, Leon Huynh, Karolina Roszak, Sangwan Park, Kevin Choy, Sina Farsiu, Ala Moshiri, Sara M Thomasy, Glenn Yiu
PURPOSE: Choroidal vascular changes occur with normal aging and age-related macular degeneration (AMD). Here, we evaluate choroidal thickness and vascularity in aged rhesus macaques to better understand the choroid's role in this nonhuman primate model of AMD. METHODS: We analyzed optical coherence tomography (OCT) images of 244 eyes from 122 rhesus macaques (aged 4-32 years) to measure choroidal thickness (CT) and choroidal vascularity index (CVI). Drusen number, size, and volume were measured by semiautomated annotation and segmentation of OCT images...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37768273/choriocapillaris-impairment-is-associated-with-delayed-rod-mediated-dark-adaptation-in-age-related-macular-degeneration
#3
JOURNAL ARTICLE
Deepayan Kar, Giulia Corradetti, Thomas A Swain, Mark E Clark, Gerald McGwin, Cynthia Owsley, SriniVas R Sadda, Christine A Curcio
PURPOSE: Progress toward treatment and prevention of age-related macular degeneration (AMD) requires imaging end points that relate to vision. We investigated choriocapillaris flow signal deficits (FD%) and visual function in eyes of individuals aged ≥60 years, with and without AMD. METHODS: One eye of each participant in the baseline visit of the Alabama Study on Early Age-Related Macular Degeneration 2 (ALSTAR2; NCT04112667) was studied. AMD presence and severity was determined using the Age-Related Eye Disease Study (AREDS) grading system...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37768272/extracellular-vesicle-micrornas-from-corneal-stromal-stem-cell-enhance-stemness-of-limbal-epithelial-stem-cells-by-targeting-the-notch-pathway
#4
JOURNAL ARTICLE
Leying Wang, Xizhan Xu, Qiankun Chen, Yuan Wei, Zhenyu Wei, Zi-Bing Jin, Qingfeng Liang
PURPOSE: The limbal niche supports the self-renewal of limbal epithelial stem cells (LESCs). The corneal stromal stem cell (CSSC) is an important component in the niche that regulates the LESC phenotype. However, the intercellular communication between LESCs and CSSCs has yet to be elucidated. METHODS: A traditional two-dimensional (2D) system, a direct three-dimensional (3D) system, and an indirect 3D coculture system of LESCs and CSSCs were used to elucidate the paracrine pathway effect of CSSCs on LESCs...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37750741/dasatinib-plus-quercetin-alleviates-choroid-neovascularization-by-reducing-the-cellular-senescence-burden-in-the-rpe-choroid
#5
JOURNAL ARTICLE
Yinhao Wang, Yulin Tseng, Keyu Chen, Kuangqi Chen, Kai Chen, Zhihua Huang, Zebin Mao, Xuemin Li
PURPOSE: Wet AMD (wAMD) is associated with cellular senescence. However, senescent cell-targeted therapies for wAMD have rarely been comprehensively studied. This study aimed to explore the therapeutic effects of senolytic agents on choroidal neovascularization (CNV). METHODS: RNA sequencing datasets were obtained from the Gene Expression Omnibus database and used to explore the association between senescence and wAMD. We explored the effects of senescent adult RPE cell line-19 cells on the proliferation, migration, invasion, and tube formation of human umbilical vein endothelial cells...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37750740/role-of-fibroblast-growth-factor-receptor-2-fgfr2-in-corneal-stromal-thinning
#6
JOURNAL ARTICLE
Roy Joseph, Akosua Boateng, Om P Srivastava, Roswell R Pfister
PURPOSE: To determine the role of fibroblast growth factor receptor 2 (FGFR2)-mediated signaling in keratocytes during corneal development, a keratocyte-specific FGFR2-knockout (named FGFR2cKO) mouse model was generated, and its phenotypic characteristics were determined. METHODS: A FGFR2cKO mouse model was generated by the following method: FGFR2 flox mice were crossed with the inducible keratocyte specific-Cre mice (Kera-rtTA/tet-O-Cre). Both male and female FGFR2cKO- and control mice (1 to 3-months-old) were analyzed for changes in corneal topography and pachymetry maps using the optical coherence tomography (OCT) method...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37747403/comprehensive-genetic-analysis-unraveled-the-missing-heritability-and-a-founder-variant-of-best1-in-a-chinese-cohort-with-autosomal-recessive-bestrophinopathy
#7
JOURNAL ARTICLE
Jie Shi, Lu Tian, Tengyang Sun, Xiao Zhang, Ke Xu, Yue Xie, Xiaoyan Peng, Xin Tang, Zi-Bing Jin, Yang Li
PURPOSE: To describe the genetic landscape of BEST1 for a large Chinese cohort with autosomal recessive bestrophinopathy (ARB), identify the missing heritability, and report a common Chinese founder variant. METHODS: We recruited 65 patients from 63 families with a clinical diagnosis of ARB. All patients underwent ophthalmic examinations and comprehensive genetic analyses, including Sanger DNA sequencing of BEST1 and whole genome sequencing (WGS). The effects of deep intronic variants (DIVs) on splicing were assessed using in vitro splicing assays in HEK293T cells and patient-derived peripheral blood mononuclear cells...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37747402/isolation-and-characterization-of-human-conjunctival-mesenchymal-stromal-cells-and-their-extracellular-vesicles
#8
JOURNAL ARTICLE
Laura García-Posadas, Ismael Romero-Castillo, Kieran Brennan, Margaret M McGee, Alfonso Blanco-Fernández, Yolanda Diebold
PURPOSE: The purpose of this study was to isolate and culture human conjunctival mesenchymal stromal cells (Conj-MSCs) from cadaveric donor tissue, and to obtain and characterize their extracellular vesicles (EVs) and their effect on conjunctival epithelium. METHODS: Stromal cells isolated from cadaveric donor conjunctival tissues were cultured and analyzed to determine whether they could be defined as MSCs. Expression of MSC markers was analyzed by flow cytometry...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37738060/a-simplified-model-of-activation-and-deactivation-of-human-rod-phototransduction-an-electroretinographic-study
#9
JOURNAL ARTICLE
James D Akula, Annie M Lancos, Bilal K AlWattar, Hanna De Bruyn, Ronald M Hansen, Anne B Fulton
PURPOSE: To test the hypothesis that a simple model having properties consistent with activation and deactivation in the rod approximates the whole time course of the photoresponse. METHODS: Routinely, an exponential of the form f = α·(1 - exp(-(τ·(t - teff)s-1))), with amplitude α, rate constant τ (often scaled by intensity), irreducible delay teff, and time exponent s-1, is fit to the early period of the flash electroretinogram...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37733365/alteration-of-meibum-lipidomics-profiling-in-patients-with-chronic-ocular-graft-versus-host-disease
#10
JOURNAL ARTICLE
Wenxin Zhao, Jing Yang, Yinglin Liao, Boyu Yang, Shujiao Lin, Ren Liu, Lingyi Liang
PURPOSE: To investigate the characteristics of the lipid profiling in meibum of patients with chronic ocular graft-versus-host disease (coGVHD) and to detect the potential influence of anti-inflammatory therapy on these differential lipids. METHODS: This cross-sectional study included 25 coGVHD patients and 13 non-coGVHD after allogeneic hematopoietic stem cell transplantation. Among those with coGVHD, 14 had prior topical treatment (coGVHD(T)), and 11 did not (coGVHD(WT))...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37728905/abca4-variant-c-5714-5g-a-in-trans-with-null-alleles-results-in-primary-rpe-damage
#11
JOURNAL ARTICLE
Jana Sajovic, Andrej Meglic, Zelia Corradi, Mubeen Khan, Aleš Maver, Martina Jarc Vidmar, Marko Hawlina, Frans P M Cremers, Ana Fakin
PURPOSE: To determine the disease pathogenesis associated with the frequent ABCA4 variant c.5714+5G>A (p.[=,Glu1863Leufs*33]). METHODS: Patient-derived photoreceptor precursor cells were generated to analyze the effect of c.5714+5G>A on splicing and perform a quantitative analysis of c.5714+5G>A products. Patients with c.5714+5G>A in trans with a null allele (i.e., c.5714+5G>A patients; n = 7) were compared with patients with two null alleles (i.e...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37728904/post-illumination-pupil-response-and-sleep-quality-in-patients-with-glaucoma-the-light-study
#12
JOURNAL ARTICLE
Hironobu Jimura, Tadanobu Yoshikawa, Kenji Obayashi, Kimie Miyata, Keigo Saeki, Nahoko Ogata
PURPOSE: This study aimed to investigate whether intrinsically photosensitive retinal ganglion cell function evaluated using post-illumination pupil response (PIPR) in patients with glaucoma is associated with sleep quality. METHODS: This cross-sectional study measured the PIPR in 138 patients with glaucoma (mean age, 70.3 years) using pupil diameter after red and blue light exposure. The net PIPR change was classified into three groups according to tertiles (i...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37725382/causal-effects-of-gut-microbiota-on-age-related-macular-degeneration-a-mendelian-randomization-study
#13
JOURNAL ARTICLE
Deshen Mao, Borui Tao, Shuyan Sheng, Hui Jin, Wenxuan Chen, Huimin Gao, Jianyi Deng, Zhuo Li, Fan Chen, Shixin Chan, Longqi Qian
PURPOSE: Recently, the association between gut microbiota and age-related macular degeneration (AMD) through the gut-retina axis has attracted great interest. However, the causal relationship between them has not been elucidated. Using publicly available genome-wide association study summary statistics, we conducted a two-sample Mendelian randomization (MR) analysis to examine the causal relationship between the gut microbiota and the occurrence of AMD. METHODS: The study used a variety of quality control techniques to select instrumental single nucleotide polymorphisms (SNPs) with strong exposure associations...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37721740/bacillus-derived-manganese-superoxide-dismutase-relieves-ocular-surface-inflammation-and-damage-by-reducing-oxidative-stress-and-apoptosis-in-dry-eye
#14
JOURNAL ARTICLE
Hyeon-Jeong Yoon, Rujun Jin, Hee Su Yoon, Ji Suk Choi, Yenny Kim, Seung Hyun Pan, Inik Chang, Lan Li, Ying Li, Jonghwa Kim, Kyung Chul Yoon
PURPOSE: We hypothesized that antioxidative enzymes supplementation could be a treatment option for dry eye. We investigated the efficacy of oral administration of Bacillus-derived superoxide dismutase (Bd-SOD) in a murine experimental dry eye (EDE). METHODS: In part I, mice were randomly assigned to normal control, EDE, and mice groups that were treated with oral Bd-SOD after induction of EDE (EDE + Bd-SOD group; four mice in each group). Expression of SOD2, a major antioxidant enzyme with manganese as a cofactor, was assessed by immunofluorescence staining...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37721739/genetic-risk-score-analysis-supports-a-joint-view-of-two%C3%A2-classification-systems-for-age-related-macular-degeneration
#15
JOURNAL ARTICLE
Janina M Herold, Martina E Zimmermann, Mathias Gorski, Felix Günther, Bernhard H F Weber, Horst Helbig, Klaus J Stark, Iris M Heid, Caroline Brandl
PURPOSE: The purpose of this study was to evaluate the utility of combining the Clinical Classification (CC) and the Three Continent age-related macular degeneration (AMD) Consortium Severity Scale (3CACSS) for classification of AMD. METHODS: In two independent cross-sectional datasets of our population-based AugUR study (Altersbezogene Untersuchungen zur Gesundheit der Universität Regensburg), we graded AMD via color fundus images applying two established classification systems (CC and 3CACSS)...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37713206/therapeutic-effects-of-upadacitinib-on-experimental-autoimmune-uveitis-insights-from-single-cell-analysis
#16
JOURNAL ARTICLE
Zhaohao Huang, Qi Jiang, Junjie Chen, Xiuxing Liu, Chenyang Gu, Tianyu Tao, Jianjie Lv, Zhaohuai Li, Zuohong Li, Wenru Su
PURPOSE: This study aimed to elucidate the impact of upadacitinib, a Janus kinase 1 (JAK1)-specific inhibitor, on experimental autoimmune uveitis (EAU) and explore its underlying mechanisms. METHODS: We utilized single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) to investigate the JAK/signal transducer and activator of transcription (STAT) pathway in peripheral blood mononuclear cells (PBMCs) of 12 patients with Vogt-Koyanagi-Harada (VKH) disease and cervical draining lymph node (CDLN) cells of EAU...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37713205/cone-density-distribution-and-related-factors-in-patients-receiving-hydroxychloroquine-treatment
#17
JOURNAL ARTICLE
Jun Tang, Hua Liu, Shiyan Mo, Zhihong Zhu, Houbin Huang, Xiaofei Liu
PURPOSE: Hydroxychloroquine is an effective treatment for rheumatic diseases; however, retinal damage is a possible side effect. We aimed to identify the retinal area and related risk factors associated with cone density reduction caused by hydroxychloroquine. METHODS: We recorded the retinal images of patients with rheumatic diseases taking hydroxychloroquine (n = 44) and compared them with images of healthy controls (n = 107). Cone density was obtained in vertical and horizontal axes...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37707836/exploring-early-stage-retinal-neurodegeneration-in-murine-pigmentary-glaucoma-insights-from-gene-networks-and-mirna-regulation-analyses
#18
JOURNAL ARTICLE
Qingqing Gu, Aman Kumar, Michael Hook, Fuyi Xu, Akhilesh Kumar Bajpai, Athena Starlard-Davenport, Junming Yue, Monica M Jablonski, Lu Lu
PURPOSE: Glaucoma is a group of heterogeneous optic neuropathies characterized by the progressive degeneration of retinal ganglion cells. However, the underlying mechanisms have not been understood completely. We aimed to elucidate the genetic network associated with the development of pigmentary glaucoma with DBA/2J (D2) mouse model of glaucoma and corresponding genetic control D2-Gpnmb (D2G) mice carrying the wild type (WT) Gpnmb allele. METHODS: Retinas isolated from 13 D2 and 12 D2G mice were subdivided into 2 age groups: pre-onset (1-6 months: samples were collected at approximately 1-2, 2-4, and 5-6 months) and post-onset (7-15 months: samples were collected at approximately 7-9, 10-12, and 13-15 months) glaucoma were compared...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37707835/ophthalmologic-phenotype-genotype-correlations-in-patients-with-oculocutaneous-albinism-followed-in-a-reference-center
#19
JOURNAL ARTICLE
Paul-Henri Seguy, Jean-François Korobelnik, Marie-Noëlle Delyfer, Vincent Michaud, Benoit Arveiler, Eulalie Lasseaux, Sarra Gattoussi, Marie-Bénédicte Rougier, Kilian Trin, Fanny Morice-Picard, Nathalie Ghomashchi, Valentine Coste
PURPOSE: Albinism is a group of genetic disorders that includes several conditions related to a defect in melanin production. There is a broad phenotypic and genotypic variability between the different forms. The aim of this study was to assess the ophthalmologic characteristics according to patients' genotypes in a cohort followed in the Reference Center for oculocutaneous albinism (OCA) of Bordeaux University Hospital, France. METHODS: A retrospective observational study was conducted in a cohort of patients with OCA seen in consultation in the ophthalmology department between 2017 and 2021 in whom a genetic analysis was performed...
September 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37707834/regulation-of-axon-guidance-by-slit2-and-netrin-1-signaling-in-the-lacrimal-gland-of-aqp5-knockout-mice
#20
JOURNAL ARTICLE
Ying Bai, Guohu Di, Huanhuan Ge, Bin Li, Kaier Zhang, Di Zhang, Dianqiang Wang, Peng Chen
PURPOSE: Dry eye disease (DED) is multifactorial and associated with nerve abnormalities. We explored an Aquaporin 5 (AQP5)-deficiency-induced JunB activation mechanism, which causes abnormal lacrimal gland (LG) nerve distribution through Slit2 upregulation and Netrin-1 repression. METHODS: Aqp5 knockout (Aqp5-/-) and wild-type (Aqp5+/+) mice were studied. LGs were permeabilized and stained with neuronal class III β-tubulin, tyrosine hydroxylase (TH), vasoactive intestinal peptide (VIP), and calcitonin gene-related peptide (CGRP)...
September 1, 2023: Investigative Ophthalmology & Visual Science
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