journal
Journals Applied and Environmental Micr...

Applied and Environmental Microbiology

https://read.qxmd.com/read/38757978/increased-resistance-against-tellurite-is-conferred-by-a-mutation-in-the-promoter-region-of-uncommon-tellurite-resistance-gene-tehb-in-the-ter-negative-shiga-toxin-producing-escherichia-coli-o157-h7
#1
JOURNAL ARTICLE
Yuko Matsumoto, Kenichi Lee, Ryuya Akasaka, Hayato Honjo, Mitsumasa Koizumi, Toshio Sato, Akiko Kubomura, Nozomi Ishijima, Yukihiro Akeda, Makoto Ohnishi, Sunao Iyoda
UNLABELLED: Resistance to potassium tellurite (PT) is an important indicator in isolating Shiga toxin-producing Escherichia coli (STEC) O157:H7 and other major STEC serogroups. Common resistance determinant genes are encoded in the ter gene cluster. We found an O157:H7 isolate that does not harbor ter but is resistant to PT. One nonsynonymous mutation was found in another PT resistance gene, tehA , through whole-genome sequence analyses. To elucidate the contribution of this mutation to PT resistance, complementation of tehA and the related gene tehB in isogenic strains and quantitative RT‒PCR were performed...
May 17, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38757977/priority-effects-alter-microbiome-composition-and-increase-abundance-of-probiotic-taxa-in-treefrog-tadpoles
#2
JOURNAL ARTICLE
Korin Rex Jones, Lisa K Belden, Myra C Hughey
UNLABELLED: Host-associated microbial communities, like other ecological communities, may be impacted by the colonization order of taxa through priority effects. Developing embryos and their associated microbiomes are subject to stochasticity during colonization by bacteria. For amphibian embryos, often developing externally in bacteria-rich environments, this stochasticity may be particularly impactful. For example, the amphibian microbiome can mitigate lethal outcomes from disease for their hosts; however, this may depend on microbiome composition...
May 17, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38752833/determination-of-soil-phenanthrene-degradation-through-a-fungal-bacterial-consortium
#3
JOURNAL ARTICLE
Chunling Luo, Guoqing Guan, Yeliang Dai, Xixi Cai, Qihui Huang, Jibing Li, Gan Zhang
Fungal-bacterial consortia enhance organic pollutant removal, but the underlying mechanisms are unclear. We used stable isotope probing (SIP) to explore the mechanism of bioaugmentation involved in polycyclic aromatic hydrocarbon (PAH) biodegradation in petroleum-contaminated soil by introducing the indigenous fungal strain A spergillus sp. LJD-29 and the bacterial strain P seudomonas XH-1. While each strain alone increased phenanthrene (PHE) degradation, the simultaneous addition of both strains showed no significant enhancement compared to treatment with XH-1 alone...
May 16, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38752748/enhancing-probiotic-impact-engineering-saccharomyces-boulardii-for-optimal-acetic-acid-production-and-gastric-passage-tolerance
#4
JOURNAL ARTICLE
Bruna Trindade de Carvalho, Ana Subotić, Paul Vandecruys, Sara Deleu, Séverine Vermeire, Johan M Thevelein
Saccharomyces boulardii has been a subject of growing interest due to its potential as a probiotic microorganism with applications in gastrointestinal health, but the molecular cause for its probiotic potency has remained elusive. The recent discovery that S. boulardii contains unique mutations causing high acetic acid accumulation and inhibition of bacterial growth provides a possible clue. The natural S. boulardii isolates Sb.P and Sb.A are homozygous for the recessive mutation whi2 S270* and accumulate unusually high amounts of acetic acid, which strongly inhibit bacterial growth...
May 16, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38752746/the-genes-mgte-and-spovg-are-involved-in-zinc-tolerance-of-staphylococcus-aureus
#5
JOURNAL ARTICLE
Jacqueline Schürmann, Martin A Fischer, Martin Herzberg, Thorsten Reemtsma, Birgit Strommenger, Guido Werner, Christopher F Schuster, Franziska Layer-Nicolaou
UNLABELLED: Metals are essential for all living organisms, but the type of metal and its concentration determines its action. Even low concentrations of metals may have toxic effects on organisms and therefore exhibit antimicrobial activities. In this study, we investigate the evolutionary adaptation processes of Staphylococcus aureus to metals and common genes for metal tolerance. Laboratory and clinical isolates were treated with manganese, cobalt, zinc, or nickel metal salts to generate growth-adapted mutants...
May 16, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38747588/determining-glycosyltransferase-functional-order-via-lethality-due-to-accumulated-o-antigen-intermediates-exemplified-with-shigella-flexneri-o-antigen-biosynthesis
#6
JOURNAL ARTICLE
Jilong Qin, Yaoqin Hong, Makrina Totsika
The O antigen (OAg) polysaccharide is one of the most diverse surface molecules of Gram-negative bacterial pathogens. The structural classification of OAg, based on serological typing and sequence analysis, is important in epidemiology and the surveillance of outbreaks of bacterial infections. Despite the diverse chemical structures of OAg repeating units (RUs), the genetic basis of RU assembly remains poorly understood and represents a major limitation in assigning gene functions in polysaccharide biosynthesis...
May 15, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38742897/discovery-of-novel-treponemes-associated-with-pododermatitis-in-elk-cervus-canadensis
#7
JOURNAL ARTICLE
Sushanta Deb, Margaret A Wild, Thomas LeClair, Devendra H Shah
Pododermatitis, also known as treponeme-associated hoof disease (TAHD), presents a significant challenge to elk ( Cervus canadensis ) populations in the northwestern USA, with Treponema spp. consistently implicated in the lesion development. However, identifying species-specific Treponema strains from these lesions is hindered by its culture recalcitrance and limited genomic information. This study utilized shotgun sequencing, in silico genome reconstruction, and comparative genomics as a culture-independent approach to identify metagenome-assembled Treponema genomes (MATGs) from skin scraping samples collected from captive elk experimentally challenged with TAHD...
May 14, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38727223/engineering-a-coenzyme-independent-dioxygenase-for-one-step-production-of-vanillin-from-ferulic-acid
#8
JOURNAL ARTICLE
Shizuka Fujimaki, Satsuki Sakamoto, Shota Shimada, Kuniki Kino, Toshiki Furuya
UNLABELLED: Vanillin is one of the world's most important flavor and fragrance compounds used in foods and cosmetics. In plants, vanillin is reportedly biosynthesized from ferulic acid via the hydratase/lyase-type enzyme VpVAN. However, in biotechnological and biocatalytic applications, the use of VpVAN limits the production of vanillin. Although microbial enzymes are helpful as substitutes for plant enzymes, synthesizing vanillin from ferulic acid in one step using microbial enzymes remains a challenge...
May 10, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38727222/the-mitochondrial-carboxylase-pcca-interacts-with-listeria-monocytogenes-phospholipase-plcb-to-modulate-bacterial-survival
#9
JOURNAL ARTICLE
Jing Wang, Mingzhu Cui, Yucong Liu, Mianmian Chen, Jiali Xu, Jing Xia, Jing Sun, Lingli Jiang, Weihuan Fang, Houhui Song, Changyong Cheng
UNLABELLED: Listeria monocytogenes , a prominent foodborne pathogen responsible for zoonotic infections, owes a significant portion of its virulence to the presence of the phospholipase PlcB. In this study, we performed an in-depth examination of the intricate relationship between L. monocytogenes PlcB and host cell mitochondria, unveiling a novel participant in bacterial survival: the mitochondrial carboxylase propionyl-coenzyme A carboxylase (PCCA). Our investigation uncovered previously unexplored levels of interaction and colocalization between PCCA and PlcB within host cells, with particular emphasis on the amino acids 504-508 of PCCA, which play a pivotal role in this partnership...
May 10, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38722179/the-elimination-of-two-restriction-enzyme-genes-allows-for-electroporation-based-transformation-and-crispr-cas9-based-base-editing-in-the-non-competent-gram-negative-bacterium-acinetobacter-sp-tol-5
#10
JOURNAL ARTICLE
Masahito Ishikawa, Katsutoshi Hori
Environmental isolates are promising candidates for new chassis of synthetic biology because of their inherent capabilities, which include efficiently converting a wide range of substrates into valuable products and resilience to environmental stresses; however, many remain genetically intractable and unamenable to established genetic tools tailored for model bacteria. Acinetobacter sp. Tol 5, an environmentally isolated Gram-negative bacterium, possesses intriguing properties for use in synthetic biology applications...
May 9, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38722171/erratum-for-stice-et-al-pantailocins-phage-derived-bacteriocins-from-pantoea-ananatis-and-pantoea-stewartii-subsp-indologenes
#11
JOURNAL ARTICLE
Shaun P Stice, Hsiao-Hsuan Jan, Hsiao-Chun Chen, Linda Nwosu, Gi Yoon Shin, Savannah Weaver, Teresa Coutinho, Brian H Kvitko, David A Baltrus
No abstract text is available yet for this article.
May 9, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38722170/accumulation-of-resistance-genes-in-salmonella-typhimurium-transmitted-between-poultry-and-dairy-farms-increases-the-risk-to-public-health
#12
JOURNAL ARTICLE
Janet Perry, Katya Arnold, Cinthia Satuchne, Ori Koren, Gaby Kenigswald, Ehud Elnekave
Salmonella Typhimurium is a zoonotic pathogen that poses a major threat to public health. This generalist serotype can be found in many hosts and the environment where varying selection pressures may result in the accumulation of antimicrobial resistance determinants. However, the transmission of this serotype between food-producing hosts, specifically between poultry layer flocks and nearby dairy herds, was never demonstrated. We investigated an outbreak at a dairy in Israel to determine the role of nearby poultry houses to be sources of infection...
May 9, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38717126/speed-dependent-bacterial-surface-swimming
#13
JOURNAL ARTICLE
Qiuqian Liu, Chi Zhang, Rongjing Zhang, Junhua Yuan
UNLABELLED: Solid surfaces submerged in liquid in natural environments alter bacterial swimming behavior and serve as platforms for bacteria to form biofilms. In the initial stage of biofilm formation, bacteria detect surfaces and increase the intracellular level of the second messenger c-di-GMP, leading to a reduction in swimming speed. The impact of this speed reduction on bacterial surface swimming remains unclear. In this study, we utilized advanced microscopy techniques to examine the effect of swimming speed on bacterial surface swimming behavior...
May 8, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38712953/correction-for-fidopiastis-et-al-vibrio-fischeri-possesses-xds-and-dns-nucleases-that-differentially-influence-phosphate-scavenging-aggregation-competence-and-symbiotic-colonization-of-squid
#14
JOURNAL ARTICLE
Pat M Fidopiastis, Chaz Childs, Jeremy J Esin, Jordan Stellern, Anna Darin, Andrea Lorenzo, Vanessa T Mariscal, Jason Lorenz, Vinay Gopan, Sarah McAnulty, Karen L Visick
No abstract text is available yet for this article.
May 7, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38712952/corrected-and-republished-from-vibrio-fischeri-possesses-xds-and-dns-nucleases-that-differentially-influence-phosphate-scavenging-aggregation-competence-and-symbiotic-colonization-of-squid
#15
JOURNAL ARTICLE
Pat M Fidopiastis, Chaz Childs, Jeremy J Esin, Jordan Stellern, Anna Darin, Andrea Lorenzo, Vanessa T Mariscal, Jason Lorenz, Vinay Gopan, Sarah McAnulty, Karen L Visick
Cells of Vibrio fischeri colonize the light organ of Euprymna scolopes , providing the squid bioluminescence in exchange for nutrients and protection. The bacteria encounter DNA-rich mucus throughout their transition to a symbiotic lifestyle, leading us to hypothesize a role for nuclease activity in the colonization process. In support of this, we detected abundant extracellular nuclease activity in growing cells of V. fischeri . To discover the gene(s) responsible for this activity, we screened a V. fischeri transposon mutant library for nuclease-deficient strains...
May 7, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38709099/single-cell-analysis-reveals-an-active-and-heterotrophic-microbiome-in-the-guaymas-basin-deep-subsurface-with-significant-inorganic-carbon-fixation-by-heterotrophs
#16
JOURNAL ARTICLE
Nicolette R Meyer, Yuki Morono, Anne E Dekas
UNLABELLED: The marine subsurface is a long-term sink of atmospheric carbon dioxide with significant implications for climate on geologic timescales. Subsurface microbial cells can either enhance or reduce carbon sequestration in the subsurface, depending on their metabolic lifestyle. However, the activity of subsurface microbes is rarely measured. Here, we used nanoscale secondary ion mass spectrometry (nanoSIMS) to quantify anabolic activity in 3,203 individual cells from the thermally altered deep subsurface in the Guaymas Basin, Mexico (3-75 m below the seafloor, 0-14°C)...
May 6, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38709098/the-biotechnological-potential-of-the-chloroflexota-phylum
#17
REVIEW
André Freches, Joana Costa Fradinho
In the next decades, the increasing material and energetic demand to support population growth and higher standards of living will amplify the current pressures on ecosystems and will call for greater investments in infrastructures and modern technologies. A valid approach to overcome such future challenges is the employment of sustainable bio-based technologies that explore the metabolic richness of microorganisms. Collectively, the metabolic capabilities of Chloroflexota , spanning aerobic and anaerobic conditions, thermophilic adaptability, anoxygenic photosynthesis, and utilization of toxic compounds as electron acceptors, underscore the phylum's resilience and ecological significance...
May 6, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38709097/semi-rational-design-of-nitroarene-dioxygenase-for-catalytic-ability-toward-2-4-dichloronitrobenzene
#18
JOURNAL ARTICLE
Jia Xu, Tao Li, Wei E Huang, Ning-Yi Zhou
Rieske non-heme dioxygenase family enzymes play an important role in the aerobic biodegradation of nitroaromatic pollutants, but no active dioxygenases are available in nature for initial reactions in the degradation of many refractory pollutants like 2,4-dichloronitrobenzene (24DCNB). Here, we report the engineering of hotspots in 2,3-dichloronitrobenzene dioxygenase from Diaphorobacter sp. strain JS3051, achieved through molecular dynamic simulation analysis and site-directed mutagenesis, with the aim of enhancing its catalytic activity toward 24DCNB...
May 6, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38700332/validation-of-aminodeoxychorismate-synthase-and-anthranilate-synthase-as-novel-targets-for-bispecific-antibiotics-inhibiting-conserved-protein-protein-interactions
#19
JOURNAL ARTICLE
Franziska Jasmin Funke, Sandra Schlee, Reinhard Sterner
UNLABELLED: Multi-resistant bacteria are a rapidly emerging threat to modern medicine. It is thus essential to identify and validate novel antibacterial targets that promise high robustness against resistance-mediating mutations. This can be achieved by simultaneously targeting several conserved function-determining protein-protein interactions in enzyme complexes from prokaryotic primary metabolism. Here, we selected two evolutionary related glutamine amidotransferase complexes, aminodeoxychorismate synthase and anthranilate synthase, that are required for the biosynthesis of folate and tryptophan in most prokaryotic organisms...
May 3, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38695519/multidrug-resistance-in-salmonella-isolates-of-swine-origin-mobile-genetic-elements-and-plasmids-associated-with-cephalosporin-resistance-with-potential-transmission-to-humans
#20
JOURNAL ARTICLE
V Garrido, A Arrieta-Gisasola, L Migura-García, L Laorden, M J Grilló
The emergence of foodborne Salmonella strains carrying antimicrobial resistance (AMR) in mobile genetic elements (MGE) is a significant public health threat in a One Health context requiring continuous surveillance. Resistance to ciprofloxacin and cephalosporins is of particular concern. Since pigs are a relevant source of foodborne Salmonella for human beings, we studied transmissible AMR genes and MGE in a collection of 83 strains showing 9 different serovars and 15 patterns of multidrug resistant (MDR) previously isolated from pigs raised in the conventional breeding system of Northern Spain...
May 2, 2024: Applied and Environmental Microbiology
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