Morphologie et Embryologie

Female mice (RAP strain) were alcoholized for 30-50 days before mating and during pregnancy until killing, with a 20% solution of ethanol administered instead of drinking water. From foetuses of 16, 18 and 20 days and from newborn puppies (day 1) choroid plexuses were excised and electronmicroscopically examined. Chronic maternal alcoholization induced the lowering of glycogen content in the choroid cells of 16 day old foetuses, the swelling and vacuolization of mitochondria with the disappearance of cristae and enlargement of the Golgi complex--in the choroid cells at all the developmental stages controlled, the enlargement of intercellular spaces within the choroid epithelium and between the capillaries and the epithelial layer...

The micronucleus test was applied in a group of 36 patients with malignant disease of the blood (acute lymphoblastic leukemia, ALL, and acute non-lymphocytic leukemia, ANLL) in order to evaluate to what extent it may be relevant for the efficiency of the cytostatic treatment. To this end, the test was applied at the onset of the disease (when diagnosed) and at different intervals after initiating the cytostatic therapy. Determination of the incidence of micronucleated cells and immature cells (blasts) at the two moments of the study established a correlation between the frequency of micronucleated cells and blast cells, the response to the anticancerous treatment and survival duration, the data obtained reflecting the prognostic value of the test in some malignant hemopathies...

Two cases with coexistent chronic lymphocytic leukemia and Hodgkin's disease are reported. The first appeared disease was the chronic lymphocytic leukemia. The eventual influence of this disease on the development of the Hodgkin's disease is discussed.

The values of some morphometric cell parameters were analysed in 12 cases of large cell non-Hodgkin's lymphoma (centroblastic and immunoblastic lymphoma and histiocytic sarcomas) in order to test the utility of the morphometric method as an additional element to the histologic diagnosis and to its further accuracy. The nuclear and nucleolar area, the number of nucleoli per nuclear cross section and the nucleus to nucleolus ratio were investigated. The morphometric data were obtained on histograms using the Giemsa stained sections...

This fourth paper dealt with the analysis of cellular population systems as the main substrates of pathological processes. The analysis pointed out the reactivity of these systems, their histogenesis and dynamics, their cellular composition and reactional capacity of composing cells, the relations between different groups of cells forming the system (intrasystemic relationships), those between proliferation and differentiation-maturation, the multifactorial determinism of proliferation, differentiation, maturation and mobilization of the cells composing the system...

The paper presents ultrastructural aspects related to zygotes and embryo cytodifferentiation of Sus scrofa d., used in the biotechnology of embryo transfer, from the first division of segmentation to the stage of preimplantational blastocyst. Together with the intense cellular proliferations, the morphostructural and functional differentiation of cells takes place. First, the blastomeres are similar from an ultrastructural point of view and do not present intercellular junctions to the advanced stage of morula...

The microcirculatory bed of the anterior stomach wall has been studied in man, dog, rabbit, guinea pig and rat, starting from the fact that in the modern gastro-duodenal surgery the various sectors of this stomach part have a special importance. The stomach microvascular network has been rendered manifest by microcorrosion preparations and by intravascular injections with coloured tracers (China Ink-gelatin, silver nitrate, methylene blue). Examinations under biocular magnifying glass and microscope of the histological slides, as well as of the block-transparentized ones in tetraline, have facilitated the microvascular morphometry as well as the calculation of some hemodynamic indices of blood flow evaluation in a limited territory of the anterior stomach wall...

The blue-polychrome-tannin rapid staining (BPT-Drăgan, 1968) applied on fresh and dried vaginal smears makes possible the identification of different forms of Trichomonas vaginalis (TV) parasite. The nucleus is coloured in blue-violet and the cytoplasm in various degrees of blue-green, violet or bichromatical, with blue and violet zones at the cell periphery. In the cytoplasm, the staining allows the identification of many granules and vacuoles. All the stainable substrates have an acid character and represent morphohistochemical aspects which reflect the parasite metabolism in different functional and evolutive stages...

Four cases (3 M, 1 F) of Lennert's lymphoma were presented. The age (38-76 years), the clinical aspect and the morphopathological features were characteristic. Despite an intensive chemotherapy, 3 of the 4 patients died between 2 and 16 months (mean survival time 7 months). The controversies about the nosological delimitation and the cellular nature of Lennert's lymphoma are presented. Because of the poor prognosis, it should be considered as a high-grade malignant non-Hodgkin lymphoma.

The microscopic changes of pulmonary arteries in 18 patients with congenital cardiovascular malformations were studied on lung biopsies and appreciated by the Heath-Edwards' scale. A correlation between the nature of the malformation and the patient's age on the one hand, and the severity of arterial lesions on the other hand, was observed, suggesting the utility of the lung biopsy for a better prognosis of the surgical corrective intervention. The role of inflammatory factors in the genesis of arterial changes leading to hypertension is also discussed...

The dynamic and structural analysis of pathological processes points out their multifactorial determining, the importance of the intrinsic conditioning, the existence of a lesional sequentiality, exemplified by the chain of lesional sequences, morphologically different, the intersequential relations of cohesive and propelling type, and the discontinuity in the progress from one lesional sequence to another. Factor ensembles, ensuring and governing the start of the process and the transition from a lesional sequence to another, were analysed in point of structure, composition, role of substrates, dynamics of ensembles, roles played by ensemble components (cohesive, propelling role)...

In section of metanephros tissue obtained from 32 human embryos and fetuses, 8 to 24 weeks old, the mesangial cells differentiate beginning with the 12th week. Some of the cells from the central mass of the metanephros blastema showed endocytosis phenomena characteristic for the adult mesangial cells. In the 13th week the three cellular types from the renal corpuscle individualize, and from the 14th to the 16th week they acquired the adult morphological aspect.

Corpus adiposum buccae (c.a.b.) was studied on 42 human subjects (fetuses and adults) using microdissection under magnifying glass after injection of the great vessels of the head with plastics, transparency technique of Spalteholtz and current histological methods. In fetuses, c.a.b. appeared as a well-developed mass located between Buccinator and inferior border of the Masseter. In adults, it emanated three prolongations among which the anteriorly directed molar prolongation was more conspicuous in edentulous old people...

China ink and typhoid vaccine were used as peritoneal stimuli injected in single doses. The subsequent omental reaction was studied after several time intervals: 6 and 12 hours, 1, 2, 3, 4 and 8 days, 3 and 6 weeks. The omental reaction featured as lymphatic nodules connected to milky spots was compared with a primary immune response. The ultrastructure and development of the characteristic cells (lymphocytes, lymphoblasts, dendritic reticulum cells, tingible body macrophages and plasma cells) was investigated throughout the period from 6 h to 6 weeks...

When stage I and II mammary carcinomas and their homolateral axillary lymph nodes were grown in the same organ culture, a phenomenon of lymphocyte migration from the nodal explants to the tumoral explants in 34% of the cases was observed. This was followed by lymphocytic infiltration in and around tumor nodules and tumoral cytotoxic effects, concomitantly with lymphocytic depletion in the nodal explants. Lymphocyte migration was particularly apparent when the axillary lymph nodes showed hyperplasia of the paracortical area and/or sinus histiocytosis...

The myeloproliferative disorders (MPD) are a domain in which the bone marrow biopsy (BMB) greatly proved its utility. We have studied the histology of the bone marrow (BM) in all the four entities of MPD: chronic myeloid leukemia (CML) with its subtype, chronic megakaryocytic granulocytic myelosis (CMGM), polycythemia vera (PV), hemorrhagic thrombocythemia (HT) and myeloid metaplasia with myelofibrosis (MMM). The work presents in short some of the clinical and hematologic characters of MPD with special stress upon the histologic modifications of BM, either specific or common to all MPD entities, underlying also the criteria for differential diagnosis...

No abstract text is available yet for this article.

Histochemical analysis of some lysosomal and sarcoplasmic proteolytic enzymes was assayed in human myocardial biopsies taken from 26 cardiopathic patients subjected to open heart operations, under extracorporeal circulation and protection with cardioplegic solution and hypothermia. The investigated myocardial proteases were: cathepsin B, cysteine aminopeptidase, acid gelatinases, trypsin-like endopeptidase, chymotrypsin-like endopeptidase and neutral gelatinases. The effects of surgical interventions appreciated by comparing the myocardium fragments harvested before, and at various intervals after aorta clamping (6-90 minutes) revealed disorders in the activity and compartmentalization of all the investigated proteases, whose histochemical reactions increased between 10 and 20 minutes after aorta clamping and manifested a lowering tendency with sarcoplasmic diffusion and extracellular release at longer periods than 20 minutes...

No abstract text is available yet for this article.

The aim of this paper is to know the iron dynamics and action of the collagen-Fe2+ complex at tissular level. The results showed that the collagen-Fe2+ complex is biocompatible at tissular level and the ferrous iron entered the animal organism on the well-known metabolic pathways. When a high dose of the complex was administered, an overloading with hemosiderin of macrophages and hepatocytes was noticed.