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Archives of Virology | Page 2

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https://read.qxmd.com/read/31076910/a-comparative-phylogenomic-analysis-of-avian-avulavirus-1-isolated-from-non-avian-hosts-conquering-new-frontiers-of-zoonotic-potential-among-species
#21
Aziz Ul-Rahman, Muhammad Zubair Shabbir
A number of avian avulavirus 1 (AAvV 1) isolates have been reported from avian and non-avian hosts worldwide with varying clinical consequences. In this regard, robust surveillance coupled with advanced diagnostics, genomic analysis, and disease modelling has provided insight into the molecular epidemiology and evolution of this virus. The genomic and evolutionary characteristics of AAvV 1 isolates originating from avian hosts have been well studied, but those originating from non-avian hosts have not. Here, we report a comparative genomic and evolutionary analysis of so-far reported AAvV 1 isolates originating from hosts other than avian species (humans, mink and swine)...
May 10, 2019: Archives of Virology
https://read.qxmd.com/read/31073706/isolation-and-identification-of-singapore-grouper-iridovirus-hainan-strain-sgiv-hn-in-china
#22
Jingguang Wei, Youhua Huang, Weibin Zhu, Chen Li, Xiaohong Huang, Qiwei Qin
In recent years, with the rapid development of marine farming activities, outbreaks of viral diseases have affected the grouper aquaculture industry, causing heavy economic losses. Singapore grouper iridovirus (SGIV) is one of the most important viruses causing disease in fish. In the present study, we isolated and identified a virus from diseased groupers by coculturing the affected tissue cells with grouper spleen cells. The genome of the isolated virus shared 99.83% nucleotide sequence homology with those of SGIV reference strains in the GenBank database...
May 9, 2019: Archives of Virology
https://read.qxmd.com/read/31069513/determination-of-the-complete-genome-sequence-of-european-mountain-ash-ringspot-associated-emaravirus-from-sorbus-intermedia-reveals-two-additional-genome-segments
#23
Susanne von Bargen, Heike-Luisa Dieckmann, Thierry Candresse, Hans-Peter Mühlbach, Jenny Roßbach, Carmen Büttner
We report the complete genome sequence, comprising six single-stranded RNAs of negative orientation, of a European mountain ash ringspot-associated virus (EMARaV) isolate identified in a diseased Sorbus intermedia (Swedish whitebeam) tree exhibiting prominent chlorotic ringspots, mottle and line pattern on leaves. Since the first observation of EMARaV-like symptoms and detection of the virus in whitebeam in 2012, the tree displayed leaf symptoms every year in at least one third of its canopy, developed dieback symptoms, and showed signs of decline...
May 8, 2019: Archives of Virology
https://read.qxmd.com/read/31065853/occurrence-of-putative-endornaviruses-in-non-cultivated-plant-species-in-south-louisiana
#24
Rachel Herschlag, Cesar Escalante, Eliezer Rodrigues de Souto, Surasak Khankhum, Ryo Okada, Rodrigo A Valverde
Extraction and electrophoretic analysis of viral dsRNA from plants has been used successfully to detect infections by RNA viruses. We used this approach as an initial tool to test non-cultivated plant species for the presence of endornaviruses. Foliar samples were collected from symptomless plants in various locations within East Baton Rouge Parish, Louisiana, USA, and tested for viral dsRNA. After testing 208 plant species belonging to 74 families, five (Geranium carolinianum, Hydrocotyle umbellata, H. prolifera, Sorghum halepense, and Sisyrinchium atlanticum) yielded dsRNAs similar in size to the dsRNAs of members of the family Endornaviridae...
May 7, 2019: Archives of Virology
https://read.qxmd.com/read/31065852/hepatitis-c-virus-3b-strains-in-injection-drug-users-in-guangdong-province-china-may-have-originated-in-yunnan-province
#25
Min Wang, Qiao Liao, Ru Xu, Dandan Song, Jieting Huang, Qingzhu You, Zhengang Shan, Ke Huang, Xia Rong, Yongshui Fu
The distribution of hepatitis C virus (HCV) genotypes/subtypes varies among different populations. Here, we investigated HCV infection and its genotype distribution in injection drug users (IDUs) in Guangdong Province of China. A total of 318 IDUs from two prisons were recruited. The genotypes/subtypes of HCV in IDUs were determined by phylogenetic analysis using E1 and/or NS5B gene sequences. Our previous data on blood donors (BDs) with no history of drug use were used as control population data for comparison...
May 7, 2019: Archives of Virology
https://read.qxmd.com/read/31065851/biological-and-molecular-characterization-of-a-bacteriophage-infecting-xanthomonas-campestris-pv-campestris-isolated-from-brassica-fields
#26
Fernanda Pereira da Silva, André da Silva Xavier, Fernanda Prieto Bruckner, Rafael Reis de Rezende, Pedro Marcus Pereira Vidigal, Poliane Alfenas-Zerbini
Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot of crucifers. Here, we report a virus that infects Xcc isolated from brassica fields in Brazil. Morphological, molecular and phylogenetic analysis indicated that the isolated virus is a new member of the genus Pbunavirus, family Myoviridae, and we propose the name "Xanthomonas virus XC 2" for this virus. The isolated virus has a narrow host range, infecting only Xcc isolates, and it did not infect unrelated bacteria. These results indicate that the isolated bacteriophage is highly specific for Xcc and may be a potential agent for biological control...
May 7, 2019: Archives of Virology
https://read.qxmd.com/read/31065850/taxonomy-of-the-order-bunyavirales-update-2019
#27
Abulikemu Abudurexiti, Scott Adkins, Daniela Alioto, Sergey V Alkhovsky, Tatjana Avšič-Županc, Matthew J Ballinger, Dennis A Bente, Martin Beer, Éric Bergeron, Carol D Blair, Thomas Briese, Michael J Buchmeier, Felicity J Burt, Charles H Calisher, Chénchén Cháng, Rémi N Charrel, Il Ryong Choi, J Christopher S Clegg, Juan Carlos de la Torre, Xavier de Lamballerie, Fēi Dèng, Francesco Di Serio, Michele Digiaro, Michael A Drebot, Xiǎoméi Duàn, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Charles F Fulhorst, Aura R Garrison, George Fú Gāo, Jean-Paul J Gonzalez, Martin H Groschup, Stephan Günther, Anne-Lise Haenni, Roy A Hall, Jussi Hepojoki, Roger Hewson, Zhìhóng Hú, Holly R Hughes, Miranda Gilda Jonson, Sandra Junglen, Boris Klempa, Jonas Klingström, Chūn Kòu, Lies Laenen, Amy J Lambert, Stanley A Langevin, Dan Liu, Igor S Lukashevich, Tāo Luò, Chuánwèi Lǚ, Piet Maes, William Marciel de Souza, Marco Marklewitz, Giovanni P Martelli, Keita Matsuno, Nicole Mielke-Ehret, Maria Minutolo, Ali Mirazimi, Abulimiti Moming, Hans-Peter Mühlbach, Rayapati Naidu, Beatriz Navarro, Márcio Roberto Teixeira Nunes, Gustavo Palacios, Anna Papa, Alex Pauvolid-Corrêa, Janusz T Pawęska, Jié Qiáo, Sheli R Radoshitzky, Renato O Resende, Víctor Romanowski, Amadou Alpha Sall, Maria S Salvato, Takahide Sasaya, Shū Shěn, Xiǎohóng Shí, Yukio Shirako, Peter Simmonds, Manuela Sironi, Jin-Won Song, Jessica R Spengler, Mark D Stenglein, Zhèngyuán Sū, Sùróng Sūn, Shuāng Táng, Massimo Turina, Bó Wáng, Chéng Wáng, Huálín Wáng, Jūn Wáng, Tàiyún Wèi, Anna E Whitfield, F Murilo Zerbini, Jìngyuàn Zhāng, Lěi Zhāng, Yànfāng Zhāng, Yong-Zhen Zhang, Yújiāng Zhāng, Xueping Zhou, Lìyǐng Zhū, Jens H Kuhn
In February 2019, following the annual taxon ratification vote, the order Bunyavirales was amended by creation of two new families, four new subfamilies, 11 new genera and 77 new species, merging of two species, and deletion of one species. This article presents the updated taxonomy of the order Bunyavirales now accepted by the International Committee on Taxonomy of Viruses (ICTV).
May 7, 2019: Archives of Virology
https://read.qxmd.com/read/31055651/importance-of-tyrosine-in-the-rna-binding-domain-of-human-parainfluenza-virus-type-2-nucleoprotein-for-polymerase-activity
#28
Yusuke Matsumoto, Keisuke Ohta, Machiko Nishio
The RNA genome of human parainfluenza virus type 2 (hPIV2) is encapsidated by nucleoprotein (NP) to act as a template for RNA synthesis. We examined the importance of individual amino acids in the RNA-binding domain of hPIV2 NP for polymerase activity using a mini-replicon assay. We showed that substitution of tyrosine at amino acid position 260, located in the RNA-binding pocket of NP, severely reduced polymerase activity. The aromatic side-chain of Y260 may be required for the formation of stable contacts between nucleotides and basic amino acids, thereby affecting promoter recognition by the viral polymerase...
May 4, 2019: Archives of Virology
https://read.qxmd.com/read/31053978/development-of-a-duplex-reverse-transcription-recombinase-aided-amplification-assay-for-respiratory-syncytial-virus-incorporating-an-internal-control
#29
Juju Qi, Xinna Li, Yi Zhang, Xinxin Shen, Guowei Song, Jing Pan, Tao Fan, Ruihuan Wang, Lixin Li, Xuejun Ma
Human respiratory syncytial virus (RSV) is a common viral pathogen that causes lower respiratory tract infections in infants and children globally. In this study, we developed a duplex reverse transcription recombinase-aided amplification (duplex-rtRAA) assay containing an internal control in a single closed tube for the detection of human RSV. The internal control in the amplification effectively eliminated false-negative results and ensured the accuracy of the duplex-rtRAA system. We first developed and evaluated a universal singleplex-rtRAA assay for RSV...
May 3, 2019: Archives of Virology
https://read.qxmd.com/read/31025116/assembly-of-tomato-blistering-mosaic-virus-like-particles-using-a-baculovirus-expression-vector-system
#30
Raquel Medeiros Vasques, Roberto Franco Teixeira Correa, Leonardo Assis da Silva, Rosana Blawid, Tatsuya Nagata, Bergmann Morais Ribeiro, Daniel M P Ardisson-Araújo
The expression of several structural proteins from a wide variety of viruses in heterologous cell culture systems results in the formation of virus-like particles (VLPs). These VLPs structurally resemble the wild-type virus particles and have been used to study viral assembly process and as antigens for diagnosis and/or vaccine development. Tomato blistering mosaic virus (ToBMV) is a tymovirus that has a 6.3-kb positive-sense ssRNA genome. We have employed the baculovirus expression vector system (BEVS) for the production of tymovirus-like particles (tVLPs) in insect cells...
April 25, 2019: Archives of Virology
https://read.qxmd.com/read/31025115/characterization-and-detection-of-a-new-badnavirus-infecting-epiphyllum-spp
#31
Pingxiu Lan, Tongyan Tian, Lingling Pu, Weili Rao, Fan Li, Ruhui Li
No abstract text is available yet for this article.
April 25, 2019: Archives of Virology
https://read.qxmd.com/read/31087190/combinatory-effects-of-vaccinia-virus-vg9-and-the-stat3-inhibitor-stattic-on-cancer-therapy
#32
Runlin Yang, Lizhen Wang, Jie Sheng, Qianhuan Huang, Donghui Pan, Yuping Xu, Junjie Yan, Xinyu Wang, Ziyue Dong, Min Yang
The recombinant vaccinia virus VG9 and the STAT3 inhibitor Stattic were combined to kill cancer cells via both oncolytic activity and inhibition of STAT3 phosphorylation in cells. The combinatory anti-tumour activity of these compounds was superior to the activity of VG9 or Stattic alone in vivo. The inhibition of tumour growth occurred via increased apoptosis and autophagy pathways. Furthermore, the combinatory anti-tumour activity was more efficient than that of VG9 or Stattic alone on xenografts, especially in nude mice...
April 24, 2019: Archives of Virology
https://read.qxmd.com/read/31011817/genome-sequencing-and-characterization-of-three-bacillus-cereus-specific-phages-dk1-dk2-and-dk3
#33
Li Kong, Yu Ding, Qingping Wu, Juan Wang, Jumei Zhang, Hongye Li, Shubo Yu, Pengfei Yu, Tiantian Gao, Haiyan Zeng, Meiyan Yang, Yongjian Liang, Zhi Wang, Zhiqing Xie, Qianwen Wang
In the study, three Bacillus cereus-specific phages, named DK1, DK2 and DK3, belonging to the family Podoviridae, were isolated from Pearl River water and sludge in Guangzhou, China. The genomes of DK1, DK2 and DK3 were 27,180 bp, 26,357 bp, and 26,865 bp in length and contained 49, 45 and 46 open reading frames, respectively. Among the three phages, DK2 shared the highest genome sequence similarity (96% identity) with DK3. Genes encoding rRNA, tRNA, virulence factors and antibiotic resistance were absent in these phage genomes...
April 22, 2019: Archives of Virology
https://read.qxmd.com/read/31011816/complete-nucleotide-sequence-of-a-new-potexvirus-cnidium-virus-x-isolated-from-cnidium-officinale-in-japan
#34
H Honma, D Tsushima, H Kawakami, N Fujihara, T Tsusaka, M Kawashimo, T Nishimura, S Fuji
A flexuous virus was detected in a Cnidium officinale plant in Japan showing mosaic symptoms. The virus was assigned to the genus Potexvirus based on analysis of its complete nucleotide sequence. The genomic RNA of the virus was 5,964 nucleotides in length, excluding the 3'-terminal poly(A) tail. It contained five open reading frames (ORFs), consistent with other members of Potexvirus. The ORF sequences differ from those of previously reported potexviruses. Phylogenetic analysis indicated that the polymerase of the virus is closely related to that of strawberry mild yellow edge virus; and the CP, to those of both yam virus X and vanilla virus X...
April 22, 2019: Archives of Virology
https://read.qxmd.com/read/30993463/genomic-sequence-of-a-bohle-iridovirus-strain-isolated-from-a-diseased-boreal-toad-anaxyrus-boreas-boreas-in-a-north-american-aquarium
#35
Kuttichantran Subramaniam, Thomas B Waltzek, V Gregory Chinchar
Genomic sequence analysis of zoo ranavirus (ZRV) suggests it is a strain of Bohle iridovirus (BIV), a virus that was first detected in, and thought to be confined to, Australia. Furthermore, marked sequence similarity and genomic co-linearity among ZRV, BIV, and German gecko ranavirus (GGRV) are consistent with the view that all three are strains  of Frog virus 3, the type species of the genus Ranavirus, family Iridoviridae.
April 16, 2019: Archives of Virology
https://read.qxmd.com/read/30993462/faba-bean-polerovirus-1-fbpv-1-a-new-polerovirus-infecting-legume-crops-in-australia
#36
Fiona F Filardo, John E Thomas, Matthew Webb, Murray Sharman
A new polerovirus species with the proposed name faba bean polerovirus 1 (FBPV-1) was found in winter legume crops and weeds in New South Wales, Australia. We describe the complete genome sequence of 5,631 nucleotides, containing all putative open reading frames, from two isolates, one from faba bean (Vicia faba) and one from chickpea (Cicer arietinum). FBPV-1 has a genome organization typical of poleroviruses with six open reading frames. However, recombination analysis strongly supports a recombination event in which the 5' portion of FBPV-1, which encodes for proteins P0, P1 and P1-P2, appears to be from a novel parent with a closest nucleotide identity of only 66% to chickpea chlorotic stunt virus...
April 16, 2019: Archives of Virology
https://read.qxmd.com/read/30989381/grapevine-virus-m-a-novel-vitivirus-discovered-in-the-american-hybrid-bunch-grape-cultivar-blanc-du-bois-in-texas
#37
Olufemi J Alabi, Sheila McBride, David N Appel, Maher Al Rwahnih, Fran M Pontasch
A novel ssRNA (+) virus with molecular properties typical of members of the genus Vitivirus (family Betaflexiviridae; subfamily Trivirinae) was discovered by high-throughput sequencing in samples of the American hybrid bunch grape cultivar Blanc du Bois in Texas. The results were independently confirmed by Sanger sequencing of the virus isolate, whose genome length is 7,387 nt, excluding the polyA tail. The genome sequence contains five ORFs that are homologous and phylogenetically related to ORFs of grapevine-infecting vitiviruses...
April 15, 2019: Archives of Virology
https://read.qxmd.com/read/30982935/high-throughput-luminex-xmap-assay-for-simultaneous-detection-of-antibodies-against-rabbit-hemorrhagic-disease-virus-sendai-virus-and-rabbit-rotavirus
#38
Miaoli Wu, Lei Ma, Feng Cong, Yujun Zhu, Fengjiao Xu, Yuexiao Lian, Bihong Huang, Li Xiao, Meili Chen, Yu Zhang, Ren Huang, Pengju Guo
Rabbits are widely used as models in biological research, and the pathogen status of rabbits used in studies can directly affect the results of experiments. Serological surveillance is the common monitoring method used in laboratory animals. A rapid, sensitive, and cost-effective high-throughput Luminex xMAP assay could be an attractive alternative to labor-intensive enzyme-linked immunosorbent assay (ELISA) methods. In this study, recombinant proteins from rabbit hemorrhagic disease virus and rabbit rotavirus and whole viral lysates of Sendai virus were used as coating antigens in an xMAP assay for the simultaneous detection of antibodies against these pathogens...
April 15, 2019: Archives of Virology
https://read.qxmd.com/read/30982088/a-novel-picornavirus-in-feces-of-a-rainbow-lorikeet-trichoglossus-moluccanus-shows-a-close-relationship-to-members-of-the-genus-avihepatovirus
#39
Haoning Wang, Shixing Yang, Tongling Shan, Xiaolong Wang, Xutao Deng, Eric Delwart, Wen Zhang
A novel picornavirus, named "lorikeet picornavirus 1" (LoPV-1), was detected in a fecal sample from rainbow lorikeets using viral metagenomic analysis, and its complete genome sequence was determined and analyzed. The genome of LoPV-1 is 7862 nt long, including a 617-nt 5' UTR, a type IV IRES 5'UTR with an '8-like' motif, a 7032-nt polyprotein ORF, and a 213-nt 3' UTR. Phylogenetic analysis and pairwise asequence comparisons based on the amino acid sequences of P1, P2, and P3 indicated that LoPV-1 showed the closest relationship to two picornaviruses that were isolated recently from red-crowned cranes and clustered together with members of the genus Avihepatovirus...
April 13, 2019: Archives of Virology
https://read.qxmd.com/read/30976905/experimental-infections-of-norway-rats-with-avian-derived-low-pathogenic-influenza-a-viruses
#40
Kaci K VanDalen, Nicole M Nemeth, Nicholas O Thomas, Nicole L Barrett, Jeremy W Ellis, Heather J Sullivan, Alan B Franklin, Susan A Shriner
No abstract text is available yet for this article.
April 11, 2019: Archives of Virology
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