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Archives of Virology

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https://read.qxmd.com/read/31414286/the-major-oligosaccharyl-transferase-complex-genes-are-not-involved-in-dengue-virus-replication-in-aedes-aegypti-mosquitoes
#1
Hugo D Perdomo, Sassan Asgari
Replication of the dengue virus (DENV) genome occurs in a vesicle in the endoplasmic reticulum by a complex of host and viral proteins. Two host proteins, STT3A and STT3B, as members of the oligosaccharyl transferase complex, have been implicated in playing structural roles in the vesicle in mammalian cells, and the absence of these proteins has been shown to decrease DENV replication. Aedes aegypti is the main vector of the virus and has been used previously as a model organism to study mosquito-virus interactions...
August 14, 2019: Archives of Virology
https://read.qxmd.com/read/31414285/the-o-polysaccharide-of-escherichia-coli-f5-which-is-structurally-related-to-that-of-e-coli-o28ab-provides-cells-only-weak-protection-against-bacteriophage-attack
#2
A K Golomidova, O I Naumenko, S N Senchenkova, Y A Knirel, A V Letarov
Several types of Escherichia coli O-antigens form highly effective shields protecting the bacterial cell surface and preventing bacteriophages from interacting directly with their secondary (terminal) receptors. However, it is not clear if O-antigens of various types (O-serotypes) differ in their anti-phage protection efficacy. Here, we describe a new E. coli strain, F5, which has an E. coli O28ab-related O-antigen. Although the amount of O-antigen produced by this strain is comparable to that produced by other E...
August 14, 2019: Archives of Virology
https://read.qxmd.com/read/31401694/genome-sequence-analysis-of-vibrio-parahaemolyticus-lytic-phage-vp_r1-with-a-c3-morphotype
#3
Hongyu Ren, Zhen Li, Le Xu, Xiaoyu Li, Lili Wang, Yongping Xu
Phage Vp_R1 belongs to the family Podoviridae and has a C3 morphotype, with an elongated head with a diameter of 190 ± 1.1 nm and an ultrashort tail with a length of 9 ± 1.2 nm. The double-stranded DNA genome is 112.1 kb long, has a mol% G + C content of 40.3, contains 129 ORFs, and encodes four tRNAs. Phylogenetic analysis suggests that phage Vp_R1 is a novel member of the genus Kuravirus.
August 10, 2019: Archives of Virology
https://read.qxmd.com/read/31401693/optimized-nested-pcr-enhances-biological-diagnosis-and-phylogenetic-analysis-of-human-parvovirus-b19-infections
#4
Maxime Pichon, Clément Labois, Véronique Tardy-Guidollet, Delphine Mallet, Jean-Sébastien Casalegno, Geneviève Billaud, Bruno Lina, Pascal Gaucherand, Yahia Mekki
Diagnosis and epidemiological analysis of human parvovirus B19 (hB19V) infections are essential for disease management in severely ill patients. This study aimed to evaluate the performance of an optimized NS1-VP1u nested PCR for detection and sequencing of viruses in clinical samples using 224 clinical and five reference samples. PCR sensitivity, specificity, and positive and negative predictive values were perfect (100%). While phylogenetic analysis of a 615 bp-long fragment demonstrated that the viruses in all of the samples belonged to genotype 1, this study confirmed that this optimized PCR could detect all known hB19V with high performance...
August 10, 2019: Archives of Virology
https://read.qxmd.com/read/31399876/expression-and-detection-of-anti-hbs-antibodies-after-hepatitis-b-virus-infection-or-vaccination-in-the-context-of-protective-immunity
#5
REVIEW
Robério Amorim de Almeida Pondé
Anti-HBs antibodies develop after natural infection with hepatitis B virus (HBV) or vaccination against this virus, as a result of activation of the human immune system by the HBV surface antigen (HBsAg). Anti-HBs-positive individuals are immunologically competent against HBV infection. This immunity is determined by the antibody levels in the bloodstream after resolution of natural infection or after vaccination. Anti-HBs antibody levels have been observed to decrease to below the protective level years after natural infection or vaccination, and there is reason to doubt that protective immunity to HBV is maintained after that...
August 9, 2019: Archives of Virology
https://read.qxmd.com/read/31399875/genomic-and-spatial-variability-of-a-european-common-vole-hepevirus
#6
René Ryll, Gerald Heckel, Victor M Corman, Jan Felix Drexler, Rainer G Ulrich
Rodents host different orthohepeviruses, namely orthohepevirus C genotype HEV-C1 (rat hepatitis E virus, HEV) and the additional putative genotypes HEV-C3 and HEV-C4. Here, we screened 2,961 rodents from Central Europe by reverse transcription polymerase chain reaction (RT-PCR) and identified HEV RNA in 13 common voles (Microtus arvalis) and one bank vole (Myodes glareolus) with detection rates of 2% (95% confidence interval [CI]: 1-3.4) and 0.08% (95% CI: 0.002-0.46), respectively. Sequencing of a 279-nucleotide RT-PCR amplicon corresponding to a region within open reading frame (ORF) 1 showed a high degree of similarity to recently described common vole-associated HEV (cvHEV) sequences from Hungary...
August 9, 2019: Archives of Virology
https://read.qxmd.com/read/31392428/detection-of-feline-immunodeficiency-virus-subtypes-a-and-b-circulating-in-the-city-of-buenos-aires
#7
Miguel Huguet, Sabrina Galdo Novo, Ana Bratanich
Feline immunodeficiency virus (FIV), genus Lentivirus, is responsible for feline immunodeficiency syndrome in domestic cats. FIV has been classified into six subtypes: A, B, C, D, E and F, based on regions of the env gene as well as the gag gene. In Argentina, the circulation of subtypes B and E was reported more than two decades ago. The objective of this work was to study the FIV variants circulating presently in the city of Buenos Aires in naturally infected cats utilizing a nested PCR targeting the gag gene...
August 7, 2019: Archives of Virology
https://read.qxmd.com/read/31385116/interferon-gamma-inhibits-transmissible-gastroenteritis-virus-infection-mediated-by-an-irf1-signaling-pathway
#8
Lingling Shan, Fang Fu, Mei Xue, Xiangdong Zhu, Liang Li, Li Feng, Pinghuang Liu
Interferon gamma (IFN-γ) is best known for its ability to regulate host immune responses; however, its direct antiviral activity is less well studied. Transmissible gastroenteritis virus (TGEV) is an economically important swine enteric coronavirus and causes acute diarrhea in piglets. At present, little is known about the function of IFN-γ in the control of TGEV infection. In this study, we demonstrated that IFN-γ inhibited TGEV infection directly in ST cells and intestine epithelial IPEC-J2 cells and that the anti-TGEV activity of IFN-γ was independent of IFN-α/β...
August 5, 2019: Archives of Virology
https://read.qxmd.com/read/31385115/molecular-characterization-of-a-novel-mycovirus-from-the-plant-pathogenic-fungus-colletotrichum-gloeosporioides
#9
Ying Wang, Shuang Liu, Hong Jian Zhu, Jie Zhong
In this study, we report the molecular characterization of a novel mycovirus in a phytopathogenic fungus of the species Colletotrichum gloeosporioides, which we named "Colletotrichum gloeosporioides RNA virus 1" (CgRV1). The virus has a dsRNA genome of 2,975 bp and possesses two non-overlapping open reading frames (ORFs 1 and 2). The smaller ORF1 encodes a protein of unknown function, and the larger ORF2 encodes the RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis based on the RdRp sequence showed that CgRV1 clustered with and is closely related to unclassified mycoviruses that are distinct from members of the family Partitiviridae...
August 5, 2019: Archives of Virology
https://read.qxmd.com/read/31377889/complete-genome-sequence-and-phylogenetic-analysis-of-a-novel-aquareovirus-isolated-from-a-diseased-marbled-eel-anguilla-marmorata
#10
Hui-Wen Huang, Chiao-Hsuan Huang, Chiu-Ming Wen
Marbled eel reovirus (MERV) is an aquareovirus (AQRV) isolated from diseased marbled eels (Anguilla marmorata) with petechial skin hemorrhage. In this study, we propagated MERV in a cell line derived from the brain of Aequidens rivulatus and purified viral particles by using a discontinuous cesium chloride gradient. Genomic RNA sequences were obtained through next-generation sequencing. MERV, similar to most other AQRVs, showed the presence of 11 double-stranded RNA segments encoding 12 proteins; however, the genome sequence displayed very little similarity to known AQRV sequences...
August 3, 2019: Archives of Virology
https://read.qxmd.com/read/31377888/identification-of-the-optimal-insertion-site-for-expression-of-a-foreign-gene-in-an-infectious-hematopoietic-necrosis-virus-vector
#11
Jing-Zhuang Zhao, Li-Ming Xu, Miao Liu, Yong-Sheng Cao, Jia-Sheng Yin, Hong-Bai Liu, Tong-Yan Lu, Zhen-Yu Zhang
Infectious hematopoietic necrosis virus (IHNV) was developed as a vector to aid the construction of vaccines against viral diseases such as viral hemorrhagic septicemia virus, spring viremia of carp virus, and influenza virus H1N1. However, the optimal site for foreign gene expression in the IHNV vector has not been determined. In the present study, five recombinant viruses with the green fluorescence protein (GFP) gene inserted into different genomic junction regions of the IHNV genomic sequence were generated using reverse genetics technology...
August 3, 2019: Archives of Virology
https://read.qxmd.com/read/31377887/complete-genome-sequence-of-a-novel-mitovirus-from-the-ectomycorrhizal-fungus-geopora-sumneriana
#12
Ergin Sahin, Ilgaz Akata
A double-stranded RNA (dsRNA) segment was extracted from the ectomycorrhizal fungus Geopora sumneriana (Cooke) M. Torre, and its full-length cDNA sequence, comprising 3146 nucleotides, was determined. Sequence analysis revealed the presence of a large open reading frame (ORF) on the positive strand of this dsRNA segment when the mold mitochondrial genetic code was applied. The ORF encodes a putative RNA-dependent RNA polymerase (RdRp), which shares the highest degree of similarity with Tuber excavatum mitovirus, with 37...
August 3, 2019: Archives of Virology
https://read.qxmd.com/read/31375908/the-complete-genome-sequence-of-apple-rootstock-virus-a-a-novel-nucleorhabdovirus-identified-in-apple-rootstocks
#13
Dasom Baek, Seungmo Lim, Ho-Jong Ju, Hyun-Ran Kim, Su-Heon Lee, Jae Sun Moon
We report the complete genome sequence of a novel nucleorhabdovirus, apple rootstock virus A (ApRVA), isolated from Malus spp. in South Korea. ApRVA has a 14,043-nt single-stranded negative-sense RNA genome. In the antigenome sense, it contains seven open reading frames, encoding the putative nucleocapsid protein, phosphoprotein, cell-to-cell movement protein, matrix protein, glycoprotein, RNA-dependent RNA polymerase, and an additional hypothetical protein, the gene for which is located between the genes for the matrix protein and glycoprotein...
August 2, 2019: Archives of Virology
https://read.qxmd.com/read/31372754/raoultella-bacteriophage-rp180-a-new-member-of-the-genus-kagunavirus-subfamily-guernseyvirinae
#14
Mikhail V Fofanov, Vera V Morozova, Yuliya N Kozlova, Artem Yu Tikunov, Igor V Babkin, Yuliya E Poletaeva, Elena I Ryabchikova, Nina V Tikunova
A novel lytic Raoultella phage, RP180, was isolated and characterized. The RP180 genome has 44,851 base pairs and contains 65 putative genes, 35 of them encoding proteins whose functions were predicted based on sequence similarity to known proteins. The RP180 genome possesses a gene synteny typical of members of the subfamily Guernseyvirinae. Phylogenetic analysis of the RP180 genome and similar phage genomes revealed that phage RP180 is the first member of the genus Kagunavirus, subfamily Guernseyvirinae, that is specific for Raoultella sp...
August 1, 2019: Archives of Virology
https://read.qxmd.com/read/31367950/a-novel-ourmia-like-mycovirus-isolated-from-the-plant-pathogenic-fungus-colletotrichum-gloeosporioides
#15
Jun Guo, Jun Zi Zhu, Xin Yu Zhou, Jie Zhong, Cai Hong Li, Zhi Gang Zhang, Hong Jian Zhu
In this study, a novel mycovirus designed Colletotrichum gloeosporioides ourmia-like virus 1 (CgOLV1) was isolated from a filamentous phytopathogenic fungus, Colletotrichum gloeosporioides. The virus has a genome of 2,516 nucleotides and contains a large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis indicated that CgOLV1 is located in the ourmia-like mycovirus clade, whose members are related to plant ourmiaviruses. To the best of our knowledge, this is the first report of an ourmia-like mycovirus in C...
July 31, 2019: Archives of Virology
https://read.qxmd.com/read/31363923/genome-characterization-of-the-novel-lytic-vibrio-parahaemolyticus-phage-vb_vpp_ba6
#16
Meiyan Yang, Yongjian Liang, Runbin Su, Hanfang Chen, Jing Wang, Jumei Zhang, Yu Ding, Li Kong, Haiyan Zeng, Liang Xue, Haoming Wu, Qingping Wu
A lytic bacteriophage, designated Vibrio phage vB_VpP_BA6, was isolated from sewage collected in Guangzhou, China. The double-stranded DNA genome of phage BA6 is composed of 50,520 bp with a G+C content of 41.77%. It possesses 64 open reading frames relating to phage structure, packaging, host lysis, DNA metabolism, and additional functions. Three tRNAs genes (encoding Pro, Ile and Trp) were detected. Comparison of its genomic features and phylogenetic analysis revealed that phage BA6 is a novel member of the family Podoviridae...
July 30, 2019: Archives of Virology
https://read.qxmd.com/read/31359148/recombinase-polymerase-amplification-assay-for-rapid-detection-of-maize-chlorotic-mottle-virus-in-maize
#17
Yubing Jiao, Junyun Jiang, Mengnan An, Zihao Xia, Yuanhua Wu
Maize chlorotic mottle virus (MCMV), an important quarantine virus, causes lethal necrosis in maize when coinfected with a potyvirid, which is seriously threatening the production of maize worldwide. In this study, recombinase polymerase amplification (RPA), a novel isothermal DNA amplification and detection technique, was developed to detect MCMV in maize crops. A pair of specific primers was designed based on the conserved sequences of the MCMV coat protein region. The RT-RPA assay was carried out as an isothermal reaction at 38 °C that was complete within 30 min, and no cross-reactivity was detected with other viruses infecting maize in China...
July 29, 2019: Archives of Virology
https://read.qxmd.com/read/31350613/complete-genome-sequences-of-novel-s-deletion-variants-of-porcine-epidemic-diarrhea-virus-identified-from-a-recurrent-outbreak-on-jeju-island-south-korea
#18
Guehwan Jang, Jonghyun Park, Changhee Lee
Porcine epidemic diarrhea virus (PEDV) is a deadly epizootic swine coronavirus that is of importance to the world pork industry. Since the re-emergence of the virulent genotype 2b (G2b) in 2014, Jeju Island in South Korea has faced periodic outbreaks, leading to the occurrence of endemics in provincial herds. In this study, we examined the complete genome sequences and molecular characteristics of novel G2b PEDV variants with a two-amino-acid deletion in the neutralizing epitope of the spike (S) gene, which were concurrently identified on a re-infected farm and its neighboring farm on Jeju Island...
July 26, 2019: Archives of Virology
https://read.qxmd.com/read/31346770/detection-of-new-vitiviruses-infecting-grapevine-in-california
#19
Alfredo Diaz-Lara, Reid S Brisbane, Kamyar Aram, Deborah Golino, Maher Al Rwahnih
Recently, five new viruses from the genus Vitivirus were identified and named grapevine virus G, H, I, J and L. These viruses were targeted in a survey to evaluate their prevalence in different grapevine populations in California. Excluding a single detection of GVJ, other vitiviruses were detected infecting several grapevine selections via RT-PCR and later confirmed by sequencing. This paper represents the first report of GVG, GVH and GVI in California. In a preliminary analysis, the sequence diversity between identified isolates of GVG, GVH, GVI and GVL was investigated using distance matrices and phylogenetics...
July 25, 2019: Archives of Virology
https://read.qxmd.com/read/31346769/high-prevalence-of-occult-hepatitis-c-virus-infection-in-injection-drug-users-with-hiv-infection
#20
Tahereh Donyavi, Farah Bokharaei-Salim, Khadijeh Khanaliha, Maryam Sheikh, Mohammad-Navid Bastani, Najmeh Moradi, Roghayeh Babaei, Zahra Habib, Atousa Fakhim, Maryam Esghaei
One of the pathological forms of chronic hepatitis C is occult HCV infection (OCI), in which there is no detectable HCV RNA in plasma specimens but HCV RNA is present in PBMCs and liver biopsy specimens. The aim of this study is to estimate the prevalence of OCI in HIV-positive people who are injection drug users (IDUs). From April 2015 to August 2018, 161 Iranian IDUs with HIV infection enrolled in the study. Viral RNA was extracted from plasma and PBMC samples of participants, and the presence of HCV RNA was examined using RT nested PCR with primers from two conserved regions (5´-UTR and NS5B)...
July 25, 2019: Archives of Virology
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