Young Hoon Oh, Matthew L Becker, Kerri M Mendola, Leila H Choe, Lie Min, Kelvin H Lee, Yinges Yigzaw, Alexander Seay, Jerome Bill, Xuanwen Li, David J Roush, Steven M Cramer, Stefano Menegatti, Abraham M Lenhoff
Product association of host-cell proteins (HCPs) to monoclonal antibodies (mAbs) is widely regarded as a mechanism that can enable HCP persistence through multiple purification steps and even into the final drug substance. Discussion of this mechanism often implies that the existence or extent of persistence is directly related to the strength of binding but actual measurements of the binding affinity of such interactions remain sparse. Two separate avenues of investigation of HCP-mAb binding are reported here...
January 19, 2024: Biotechnology and Bioengineering