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Clinica Chimica Acta; International Journal of Clinical Chemistry

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https://read.qxmd.com/read/30776362/relative-quantification-of-plasma-n-glycans-in-type-ii-congenital-disorder-of-glycosylation-patients-by-mass-spectrometry
#1
E A Barbosa, N do C Fontes, S C L Santos, D J Lefeber, C Bloch, J M Brum, G D Brand
BACKGROUND: Type II Congenital Disorders of Glycosylation (CDG-II) are a group of diseases with challenging diagnostics characterized by defects in the processing of glycans in the Golgi apparatus. Mass Spectrometry (MS) has been a valuable tool in the definition of CDG-II subtypes. While some CDG-II subtypes are associated with specific N-glycan structures, others only produce changes in relative levels, reinforcing the demand for quantification methods. METHODS: Plasma samples from control individuals were pooled, derivatized with deuterated iodomethane (I-CD3 ), and used as internal standards for controls and patients whose glycans were derivatized with iodomethane (I-CH3 ), followed by MALDI MS, LC-MS and -MS/MS analyses...
February 15, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30776361/endometriosis-associated-ovarian-cancer-what-have-we-learned-so-far
#2
REVIEW
M Herreros-Villanueva, Chih-Chieh Chen, Eing-Mei Tsai, Tze-Kiong Er
Endometriosis is defined as the presence of ectopic endometrial tissue outside of the uterine cavity, most commonly in the ovaries and peritoneum. It is a complex disease that is influenced by multiple factors. It is also a common gynecological disorder and affects approximately 10-15% of all women of reproductive age. Recent molecular and pathological studies indicate that endometriosis may serve as a precursor of ovarian cancer (endometriosis-associated ovarian cancer, EAOC), particularly endometrioid and clear cell ovarian cancers...
February 15, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30776360/long-interspersed-nuclear-element-1-mobilization-as-a-target-in-cancer-diagnostics-prognostics-and-therapeutics
#3
REVIEW
Afsaneh Lavasanifar, Cierra N Sharp, Erik A Korte, Tyler Yin, Keivan Hosseinnijad, Saeed A Jortani
Long Interspersed Nuclear Element-1 (LINE-1) are DNAs that compromise 17% of our genome. LINE-1 expression is triggered by environmental stressors and accomplished through its demethylation leading to genomic instability. Expression of LINE-1 is regulated in adult somatic tissues through several endogenous defensive mechanisms but is found to be associated with tumorigenesis in several cancers. This finding, has inspired the use of different indicators of LINE-1 activation, as biomarkers in cancer diagnostics and even therapeutic targets in recent years...
February 15, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30772337/association-of-vitamin-d-deficiency-with-insulin-resistance-in-middle-aged-type-2-diabetics
#4
Neetu Mishra, Gauri Damle, Yogita Dhas, Joyita Banerjee
BACKGROUND: Vitamin D deficiency contributes to the pathophysiology of insulin resistance (IR) and type 2 diabetes mellitus (T2DM). We investigated the association of 25-hydroxyvitamin D [25(OH)D] with IR and β-cell function in middle-aged participants. METHODS: We enrolled 90 controls and 90 T2DM patients of both genders aged 30-50 y. Serum 25(OH)D, fasting plasma insulin (FPI), fasting plasma glucose (FPG), HbA1c, and lipid profile were measured by standard methods...
February 14, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30772336/gene-expression-changes-in-cervical-squamous-cancers-following-neoadjuvant-interventional-chemoembolization
#5
Yonghua Chen, Yuanyuan Hou, Ying Yang, Meixia Pan, Jing Wang, Wenshuang Wang, Ying Zuo, Jianglin Cong, Xiaojie Wang, Nan Mu, Chenglin Zhang, Benjiao Gong, Jianqing Hou, Shaoguang Wang, Liping Xu
BACKGROUND: The efficacy of therapy for cervical cancer is related to the alteration of multiple molecular events and signaling networks during treatment. The aim of this study was to evaluate gene expression alterations in advanced cervical cancers before- and after-trans-uterine arterial chemoembolization- (TUACE). METHODS: Gene expression patterns in three squamous cell cervical cancers before- and after-TUACE were determined using microarray technique. Changes in AKAP12 and CA9 genes following TUACE were validated by quantitative real-time PCR...
February 14, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30771301/hematology-reference-intervals-for-transgender-adults-on-stable-hormone-therapy
#6
Dina N Greene, Gabrielle Winston McPherson, Jessica Rongitsch, Katherine L Imborek, Robert L Schmidt, Robert M Humble, Nicole Nisly, Nancy J Dole, Susan K Dane, Janice Frerichs, Matthew D Krasowski
BACKGROUND: The complete blood count (CBC) is a cornerstone of patient care. Several of the normal values for the components of the CBC differ by sex and, therefore, male-specific and female-specific reference intervals are required to interpret these laboratory results. Transgender individuals are often prescribed hormone therapy to affirm their gender, with resulting serum hormone concentrations similar to those of cisgender individuals. Gender-specific reference intervals for transgender men and women have not been established for any laboratory measurements, including hematology...
February 13, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30771300/determination-of-serum-neutrophil-gelatinase-associated-lipocalin-as-a-prognostic-biomarker-of-acute-spontaneous-intracerebral-hemorrhage
#7
Sheng Chen, Xia-Chan Chen, Xiao-Hui Lou, Song-Quan Qian, Zhan-Wei Ruan
BACKGROUND: Neutrophil gelatinase-associated lipocalin (NGAL) is currently known as an acute phase protein and implicated in acute brain injury. Herein, we sought to gauge serum NGAL level in patients after acute (<24 h) spontaneous intracerebral hemorrhage (ICH) and to investigate its relation to neurological outcome. METHODS: Serum NGAL levels were measured in 106 patients and 106 controls. National Institutes of Health Stroke Scale (NIHSS) score, Glasgow coma scale (GCS) score, ICH score and hematoma volume were recorded for assessing hemorrhagic severity...
February 13, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30771299/validity-of-a-rapid-and-simple-fluorometric-tripeptidyl-peptidase-1-tpp1-assay-using-dried-blood-specimens-to-diagnose-cln2-disease
#8
Zoltan Lukacs, Miriam Nickel, Simona Murko, Paulina Nieves Cobos, Angela Schulz, René Santer, Alfried Kohlschütter
PURPOSE: CLN2 disease is a genetic disorder caused by dysfunction of the lysosomal enzyme tripeptidyl peptidase 1 (TPP1) that belongs to the neuronal ceroid lipofuscinoses (NCL) and leads to epilepsy, dementia, and death in young persons. CLN2 disease has recently become treatable by enzyme replacement, which can only be effective when the disease is diagnosed early. We have investigated the reliability of a test for TPP1 deficiency in dried blood specimens (DBS) to detect CLN2 disease...
February 13, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30768927/biomarkers-for-the-prediction-of-early-pulmonary-embolism-related-mortality-in-spontaneous-and-provoked-thrombotic-disease
#9
Ljiljana Jovanovic, Vesna Subota, Milena Stavric, Bojana Subotic, Boris Dzudovic, Natasa Novicic, Jovan Matijasevic, Milica Miric, Sonja Salinger, Natasa Markovic-Nikolic, Maja Nikolic, Vladimir Miloradovic, Ljiljana Kos, Tamara Kovacevic-Preradovic, Jelena Marinkovic, Nikola Kocev, Slobodan Obradovic
Factors associated with provoked PE may influence a biomarker's predictive value for the primary outcome. The aim of this study was to investigate the value of BNP, cTnI, CRP and D-Dimer measurements taken soon after hospital admission for the prediction of 30-day PE-caused death in patients with spontaneous versus provoked PE.Data were extracted from a pool of 726 consecutive PE patients enrolled in the multicenter Serbian PE registry. Blood concentrations of BNP, cTnI, CRP and D-dimer were measured during the first 24 h of hospitalization...
February 12, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30763574/predictive-value-of-preoperative-lymphocyte-to-monocyte-ratio-for-patients-with-upper-tract-urothelial-carcinoma
#10
Tao Li, Hang Xu, Lu Yang, Ping Tan, Qiang Wei
BACKGROUND: To determine the predictive value of preoperative lymphocyte-to-monocyte ratio (LMR) for pathological and survival outcomes in upper tract urothelial carcinoma (UTUC) after radical nephroureterectomy (RNU). METHODS: This retrospective study included 704 UTUC patients between 2008 and 2017. We used a cutoff LMR of 3.6 to evaluate its relationship with oncological outcomes after RNU, using the Kaplan-Meier method and logistic regression models. RESULTS: During median follow-up of 39 months, decreased preoperative LMR was an independent predictor of poor pathological outcomes...
February 11, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30738955/practical-application-of-the-sigma-metric-run-size-nomogram-for-multistage-bracketed-statistical-quality-control-analysis-of-eight-enzymes
#11
Yuping Zeng, He He, Ken Qin, Mei Zhang, Zhenmei An, Hengjian Huang
BACKGROUND: A sigma-metric run size Nomogram is used to recommend quality control (QC) strategies to reduce patient risks. Herein, we aimed to evaluate the sigma performance of 8 enzymes and apply multistage bracketed statistical QC (SQC). METHODS: Sigma performance of alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatine kinase (CK), amylase (AMY), and lipase (LIP) were determined...
February 7, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30735665/comparison-between-amyloid-pet-and-csf-amyloid-%C3%AE-biomarkers-in-a-clinical-cohort-with-memory-deficits
#12
Caroline Bouter, Jonathan Vogelgsang, Jens Wiltfang
With increasing prevalence of Alzheimer's disease (AD) and advances in research of therapeutic approaches, an early and accurate in-vivo diagnosis is crucial. Different biomarkers that are able to identify AD are currently in focus. However, whether and to which extend results of cerebrospinal fluid (CSF) and imaging biomarkers are comparable, is unclear. This study aims to correlate CSF and amyloid imaging biomarkers comparing them to cognitive measurements in order to determine whether these methods provide identical or complementary information...
February 5, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30731088/development-and-validation-of-a-lc-ms-ms-assay-for-quantification-of-serum-estradiol-using-calibrators-with-values-assigned-by-the-cdc-reference-measurement-procedure
#13
Justin Dowis, Witold Woroniecki, Deborah French
BACKGROUND: Estradiol was historically measured by gas chromatography-mass spectrometry before development of immunoassays. Although immunoassays were fast requiring low sample volume, they had specificity issues. More recently, liquid chromatography-tandem mass spectrometry became the methodology of choice for estradiol quantitation in serum. However, one thing all methods have in common is lack of standardization. METHODS: A LC-MS/MS assay was developed and clinically validated using calibrators with concentrations assigned by a Reference Measurement Procedure in an effort to increase accuracy of calibration...
February 4, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30731087/a-diethylpyrocarbonate-based-derivatization-method-for-the-lc-ms-ms-measurement-of-plasma-arginine-and-its-chemically-related-metabolites-and-analogs
#14
Salvatore Sotgia, Angelo Zinellu, Panagiotis Paliogiannis, Gerard A Pinna, Arduino A Mangoni, Luciano Milanesi, Ciriaco Carru
BACKGROUND: Changes in NO metabolism correlate with cardiovascular risk factors and are associated with endothelial dysfunction. NO availability is regulated by nitric oxide synthase (NOS) and arginine and some chemically related metabolites and analogs have the capacity to alter NOS activity. Hence the need for analytical methods for the simultaneous assessment of these analytes. METHODS: Analytes (L-arginine (Arg), NG -monomethyl-L-arginine (MMA), L-homoarginine (hArg), asymmetric dimethyl-L-arginine (ADMA), symmetric dimethyl-L-arginine (SDMA), and L-citrulline (CIT)) were isolated from human plasma by thermal coagulation of plasma followed by a derivatization with diethylpyrocarbonate...
February 4, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30726722/deamidated-gliadin-peptide-in-pediatric-patients-with-moderately-increased-tissue-transglutaminase-does-it-help
#15
Jane A Dickerson, Dale Lee, M Cristina Pacheco
BACKGROUND: Deamidated gliadin peptide (DGP) is a relatively new serologic assay used in diagnosis and monitoring of celiac disease. DGP IgG is recommended by some in pediatric patients <2 y. Use in other pediatric populations is not well established. The utility of the DGP screen (IgG + IgA) in patients with moderate increase of tissue transglutaminase (TTG) IgA has not been studied. METHODS: Cases between January 2015 and October 2017 in which a patient had TTG IgA greater >19 and <100, DGP screen, and biopsy were collected...
February 3, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30716279/using-circular-rna-smarca5-as-a-potential-novel-biomarker-for-hepatocellular-carcinoma
#16
Zuhua Li, Ye Zhou, Guohua Yang, Siying He, Xueping Qiu, Lei Zhang, Qianyun Deng, Fang Zheng
BACKGROUND: Hepatocellular carcinoma (HCC) is the most common malignant tumor worldwide. Circular RNAs (circRNAs), a new class of endogenous non-coding RNAs, are widespread and abundant in mammalian cells. Cumulative evidence showed that circRNAs play significant roles in the process of cancer. However, the expression and function of circRNAs in HCC remain to be investigated. METHODS: The expression of circular RNA SMARCA5 (circSMARCA5) in tissues and plasma samples was detected by quantitative real-time polymerase chain reaction (qRT-PCR)...
February 2, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30711524/advanced-nanotechnology-an-arsenal-to-enhance-immunotherapy-in-fighting-cancer
#17
REVIEW
Chun-Ting Cheng, Gabriel Castro, Chun-Hsin Liu, Pauline Lau
Cancer remains a major disease process with considerable healthcare and socioeconomic impact worldwide. Unfortunately, standard treatments using chemotherapy often do not effectively control cancer progression or prevent relapse. Over the past decades, the development of targeted therapies has substantially improved outcomes. Recently, immunotherapy has emerged as a new alternative for more effective cancer treatment and may even bring hope of a cure. Cancer immunotherapy functions by reinforcing a patient's immune defense system to fight the disease...
January 31, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30711523/assessment-of-access-hstni-99th-percentiles-upper-reference-limits-following-ifcc-recommendations
#18
Martina Di Pietro, Mariella Dipalo, Marco Bruno Luigi Rocchi, Roberta Musa, Paola Avanzini, Cecilia Gnocchi, Maria Chiara Anelli, Rosalia Aloe
BACKGROUND: The detection of an increase and/or decrease of cardiac troponin (cTnI) values, with at least one value above the 99th percentile of the upper reference limit (URL) have a central role in acute myocardial infarction (AMI) diagnosis. The employment of sex specific 99th percentile URLs and High-sensitivity (Hs) assays are recommended. We assessed sex specific 99th percentile URL for Access Hs-cTnI and AccuTnI3+ (Beckman Coulter) using European donor reference population following recent International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) recommendations...
January 31, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30707895/a-virtuous-diagnostic-and-therapeutic-roadmap-triggered-by-a-motivated-and-skilful-urinary-sediment-examination
#19
LETTER
G Garigali, G Marra, V Rizzo, F De Liso, A Berrettini, M Daudon, G B Fogazzi
In this paper we describe how an accurate urinary sediment examination, which revealed the presence of a severe 2,8-dihydroxyadenine crystalluria, opened the way to an articulate and successful diagnostic and therapeutic roadmap for a rare and potentially severe renal disease.
January 29, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/30703345/performance-evaluation-of-a-new-and-improved-cuvette-based-automated-urinalysis-analyzer-with-phase-contrast-microscopy
#20
Rosanna Falbo, Maria Roberta Sala, Marco Bussetti, Fabrizio Cappellini, Chiara Giacobone, Chiara Fania, Paolo Brambilla
BACKGROUND: The use of phase contrast in urinalysis has been highly recommended. A new system, sediMAX conTRUST PRO, is now available providing simultaneous automated phase contrast and bright field microscopy. This study aimed to evaluate both analytical and diagnostic performance of this new analyzer. METHODS: Results from 504 samples evaluated with the sediMAX conTRUST PRO were compared to those obtained from the same samples by manual microscopy (MM). Analytical and diagnostic performance were assessed according to established guidelines...
January 28, 2019: Clinica Chimica Acta; International Journal of Clinical Chemistry
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