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Journals Clinica Chimica Acta; Internat...

Clinica Chimica Acta; International Journal of Clinical Chemistry

https://read.qxmd.com/read/36958425/steroid-profiling-for-the-diagnosis-of-congenital-adrenal-hyperplasia-by-microbore-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#1
JOURNAL ARTICLE
Disheng Feng, Zixuan Wang, Hang Li, Xianzhe Shi, Lin Zou, Hongwei Kong, Zhiliang Xu, Chaowen Yu, Chunxiu Hu, Guowang Xu
BACKGROUND: A rapid and accurate measurement approach for 17α-hydroxyprogesterone (17-OHP) and related steroids in amount/volume-limited clinic samples is of importance for precise newborn diagnosis of congenital adrenal hyperplasia (CAH) and its subtypes in clinic. METHODS: Sixteen steroids (17-OHP, androstenedione, cortisol, tetrahydro-11-deoxycortisol, pregnenolone, progesterone, 11-deoxycorticosterone, corticosterone, 21-deoxycortisol, 11-deoxycortisol, dehydroepiandrosterone, testosterone, aldosterone, 17α-hydroxypregnenolone, dihydrotestosterone and 18-hydroxycorticosterone) were included in the panel of high-throughput microbore ultra-performance liquid chromatography-tandem mass spectrometry...
March 21, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36948239/development-and-evaluation-of-an-electrochemical-biosensor-for-creatinine-quantification-in-a-drop-of-whole-human-blood
#2
JOURNAL ARTICLE
Gabriel Álvarez Menéndez, Olaya Amor-Gutiérrez, Agustín Costa García, María Funes-Menéndez, Catuxa Prado, Diego Miguel, Pablo Rodríguez-González, Adriana González-Gago, J Ignacio García Alonso
An electrochemical biosensor for creatinine determination in a drop of whole human blood was developed and applied to the determination of creatinine in real clinical samples. It is based on the modification of a dual carbon working electrode with a combination of three enzymes: creatinine amidohydrolase (CNN), creatine amidinohydrolase (CRN) and sarcosine oxidase (SOX). Electrochemical transduction is performed using horseradish peroxidase (HRP) and potassium hexacyanoferrate(II) as mediator. A drop of human blood is enough to carry out the measurements by differential chronoamperometry where one carbon electrode detects creatine and the other both creatine and creatinine...
March 20, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36948238/simplified-urinary-steroid-profiling-by-lc-ms-as-diagnostic-tool-for-malignancy-in-adrenocortical-tumors
#3
JOURNAL ARTICLE
Nora Vogg, Tobias Müller, Andreas Floren, Thomas Dandekar, Anna Riester, Ulrich Dischinger, Max Kurlbaum, Matthias Kroiss, Martin Fassnacht
OBJECTIVES: Preoperative identification of malignant adrenal tumors is challenging. 24-h urinary steroid profiling by LC-MS/MS and machine learning has demonstrated high diagnostic power, but the unavailability of bioinformatic models for public use has limited its routine application. We here aimed to increase usability with a novel classification model for the differentiation of adrenocortical adenoma(ACA) and adrenocortical carcinoma(ACC). METHODS: Eleven steroids (5-pregnenetriol, dehydroepiandrosterone, cortisone, cortisol, α-cortolone, tetrahydro-11-deoxycortisol, etiocholanolone, pregnenolone, pregnanetriol, pregnanediol, and 5-pregnenediol) were quantified by LC-MS/MS in 24-h urine samples from 352 patients with adrenal tumor (281 ACA,71 ACC)...
March 20, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36948237/psa-reactivity-in-extracellular-microvesicles-to-commercial-immunoassays
#4
JOURNAL ARTICLE
Amaia Sandúa, Miguel F Sanmamed, María Rodríguez, Javier Ancizu-Marckert, Alfonso Gúrpide, José L Perez-Gracia, Estibaliz Alegre, Álvaro González
AIMS: Characterization of PSA in extracellular microvesicles (EVs) and its reactivity to commercial methods. MATERIALS AND METHODS: EVs derived from serum of 47 prostate cancer (PCa) patients, 27 benign prostatic hyperplasia (BPH) patients and 42 healthy controls were analyzed. EVs isolation and quantification of PSA immunoreactive to total (ev-T-PSA) or free (ev-F-PSA) PSA immunoassays, were performed using commercial assays. PSA in CD81+ or CD63+ EVs was determined directly in serum by an immunocapture-ELISA (IC-ELISA)...
March 20, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36940842/identification-of-a-novel-plasma-metabolite-panel-as-diagnostic-biomarker-for-hepatocellular-carcinoma
#5
JOURNAL ARTICLE
Zhiying Liu, Hongtao Liu, Zhiji Chen, Chao Deng, Li Zhou, Siyuan Chen, Juan Kang, Yao Chen, Song He, Zhihang Zhou
BACKGROUND AND AIMS: Metabolic reprogramming is one of the hallmarks of cancer. Hepatocellular carcinoma (HCC) is one of the most lethal malignancy camcer, but the early diagnosis of HCC remains difficult. In this study, we searched for potential plasma metabolite biomarkers of HCC. METHODS: A total of plasma samples of 104 HCC, 76 cirrhosis and 10 healthy subjects were assessed and validated through Gas chromatography-Mass spectrometry. Receiver-operating characteristic curves (ROC) combined with multivariate statistical analyses were used to assess the diagnostic performance of metabolites and combinations...
March 18, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36934953/benzodiazepine-analysis-by-an-improved-lc-ms-ms-method-illustrates-usage-patterns-in-washington-state
#6
JOURNAL ARTICLE
Ka Keung Chan, Andrea J Moore, Andrew N Hoofnagle, Geoffrey S Baird, Hsuan-Chieh Liao
BACKGROUND: The standard approach for benzodiazepine detection often includes immunoassay followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The illicit use of non-prescribed benzodiazepines has been trending up nationally. METHODS: We developed and validated an improved LC-MS/MS assay for benzodiazepine detection in urine. We expanded the testing panel by adding five drugs to the previous panel of ten. We determined the prevalence of individual benzodiazepines in our patient population...
March 17, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36931585/cerebrospinal-fluid-levels-of-the-macrophage-specific-biomarker-scd163-are-diagnostic-for-lyme-neuroborreliosis-an-observational-cohort-study
#7
JOURNAL ARTICLE
Mathilde Ørbæk, Rosa Maja Møhring Gynthersen, Helene Mens, Christian Brandt, Christian Stenør, Lothar Wiese, Åse Bengaard Andersen, Holger J Møller, Anne-Mette Lebech
OBJECTIVES: We aimed to investigate levels of the macrophage-specific marker, sCD163, in cerebrospinal fluid and plasma in patients with Lyme neuroborreliosis. We tested the diagnostic value of CSF-sCD163 and ReaScan-CXCL13 and analyzed if plasma-sCD163 could monitor treatment response. METHODS: An observational cohort study: Cohort 1-Cerebrospinal fluid from adults with neuroborreliosis (n=42), bacterial meningitis (n=16), enteroviral meningitis (n=29), and controls (n=33); Cohort 2-Plasma from 23 adults with neuroborreliosis collected at diagnosis, three, and six months...
March 15, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36925056/n-glycosylation-of-serum-proteins-in-adult-type-1-diabetes-mellitus-exposes-further-changes-compared-to-children-at-the-disease-onset
#8
JOURNAL ARTICLE
Matej Nemčić, Marko Tijardović, Najda Rudman, Tomislav Bulum, Martina Tomić, Branimir Plavša, Sandra Vučković Rebrina, Marijana Vučić Lovrenčić, Lea Duvnjak, Grant Morahan, Olga Gornik
OBJECTIVE: Previously we have shown that plasma protein N-glycosylation is changed in children at the onset of type 1 diabetes. In this study, we aim to identify N-glycan changes in adults with T1DM, compare them to those in children, and investigate their associations with disease duration, complications, glycaemic status, and smoking. METHODS: N-glycome of serum proteins from 200 adults with type 1 diabetes and 298 healthy controls were analysed using ultra-high performance liquid chromatography and divided into 39 directly measured glycan groups from which 16 derived traits were calculated...
March 14, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36921681/letter-to-the-editor-regarding-a-haemochromatosis-causing-hfe-mutation-is-associated-with-sars-cov-2-susceptibility-in-the-czech-population-clinica-chimica-acta-538-2023-211-215
#9
LETTER
Janice L Atkins, Mitchell Lucas, Luke C Pilling, David Melzer
No abstract text is available yet for this article.
March 13, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36918061/fragmentation-of-human-cardiac-troponin-t-after-acute-myocardial-infarction
#10
JOURNAL ARTICLE
Ivan A Katrukha, Natalia S Riabkova, Alexander E Kogan, Alexandra V Vylegzhanina, Kadriya Sh Mukharyamova, Agnessa P Bogomolova, Artur I Zabolotskii, Ekaterina V Koshkina, Anastasia V Bereznikova, Alexey G Katrukha
BACKGROUND: Blood measurement of cardiac troponin T (cTnT) is one of the most widespread methods of acute myocardial infarction (MI) diagnosis. cTnT degradation may have a significant influence on the precision of cTnT immunodetection; however, there are no consistent data describing the level and sites of cTnT proteolysis in the blood of MI patients. In this study, we bordered major cTnT fragments and quantified their relative abundance in the blood at different times after MI. METHODS: Serial heparin plasma samples were collected from 37 MI patients 2-37 h following the onset of MI...
March 12, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36914043/quantitative-analysis-of-ethanolamine-plasmalogen-species-in-red-blood-cells-using-liquid-chromatography-tandem-mass-spectrometry-for-diagnosing-peroxisome-biogenesis-disorders
#11
JOURNAL ARTICLE
Irene De Biase, Tatiana Yuzyuk, Wei Cui, Lauren M Zuromski, Ann B Moser, Nancy E Braverman
Plasmalogens are glycerophospholipids characterized by a vinyl-ether bond with a fatty alcohol at the sn-1 position, a polyunsaturated fatty acid at the sn-2 position, and a polar head at the sn-3 position, commonly phosphoethanolamine. Plasmalogens play crucial roles in several cellular processes. Reduced levels have been associated with Alzheimer's and Parkinson's disease progression. Markedly reduced plasmalogens are a classic feature of peroxisome biogenesis disorders (PBD) because plasmalogen synthesis requires functional peroxisomes...
March 11, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36893880/do-phi-and-phi-density-improve-detection-of-clinically-significant-prostate-cancer-only-in-the-psa-gray-zone
#12
JOURNAL ARTICLE
Rius Bilbao Leire, Valladares Gomez Carmen, Aguirre Larracoechea Urko, Pereira Arias Jose Gregorio, Arredondo Calvo Pablo, Urdaneta Salegui Luis Felipe, Escobal Tamayo Victor, Sanz Jaka Juan Pablo, Recio Ayesa Adrian, Mar Medina Javier, Mar Medina Carmen
OBJECTIVES: Prostate health index (PHI) is a predictive biomarker of positive prostate biopsy. The majority of evidence refers to its use in the PSA gray zone (4-10 ng/mL) and negative digital rectal exam (DRE). We aim to evaluate and compare the predictive accuracy of PHI and PHI density (PHId) with PSA, percentage of free PSA and PSA density, in a wider range of patients for the detection of clinically significant prostate cancer (csPCa). METHODS: Multicenter prospective study that included patients suspicious of harboring prostate cancer...
March 7, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36878379/head-and-neck-cancer-biomarkers-systematic-review-and-meta-analysis
#13
REVIEW
Vijaya Marakala
PURPOSE: To perform a systematic review and meta-analysis of the diagnostic capabilities of various biological markers in the plasma, serum, tissue, and saliva of patients with head and neck cancer (HNC). METHODS: We performed manual and digital searches using specific keywords and found English-language literature published up to October 28, 2022. PubMed, ScienceDirect, Scopus, MEDLINE Complete, and EMBASE databases were used. Studies comparing biomarkers for the diagnosis of HNC versus healthy controls were evaluated...
March 4, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36871662/evaluation-of-dried-blood-spots-and-a-hematocrit-independent-procedure-in-lysosomal-diseases-screening-using-multiplexed-tandem-mass-spectrometry-assays
#14
JOURNAL ARTICLE
Franklin Ducatez, Carine Pilon, Justine Ferey, Stéphane Marret, Soumeya Bekri, Abdellah Tebani
BACKGROUND: Dried blood spots (DBS) are widely used as a non-invasive sampling method, especially in newborn screening (NBS). Despite its numerous advantages, conventional DBS might be limited by the hematocrit effect when analyzing a punch, depending on its position in the blood spot. This effect could be avoided using hematocrit-independent sampling devices such as the hemaPEN®. This device collects blood through integrated microcapillaries, and a fixed blood volume is deposited on a pre-punched paper disc...
March 3, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36871661/designing-and-developing-a-sensitive-and-specific-sars-cov-2-rbd-igg-detection-kit-for-identifying-positive-human-samples
#15
JOURNAL ARTICLE
Ehsan Raoufi, Fatemeh Hosseini, Bahman Onagh, Mohammad Salehi-Shadkami, Marjan Mehrali, Monireh Mohsenzadegan, Jim Q Ho, Banafsheh Bigdelou, Mohammad Reza Sepand, Thomas J Webster, Steven Zanganeh, Mohammad M Farajollahi
BACKGROUND: More than 3 y into the coronavirus 2019 (COVID-19) pandemic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to undergo mutations. In this context, the Receptor Binding Domain (RBD) is the most antigenic region among the SARS-CoV-2 Spike protein and has emerged as a promising candidate for immunological development. We designed an IgG-based indirect enzyme-linked immunoassay (ELISA) kit based on recombinant RBD, which was produced from the laboratory to 10 liter industry scales in Pichia pastoris...
March 3, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36870523/hemolysis-or-wine-a-case-of-red-wine-colored-plasma
#16
Hind Malaeb, Michael A Vera, Cristina Figueroa Villalba, Joe M El-Khoury
No abstract text is available yet for this article.
March 2, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36870522/lipoprotein-lipase-concentration-in-umbilical-cord-blood-reflects-neonatal-birth-weight
#17
JOURNAL ARTICLE
Rieko Sagara, Satoshi Hirayama, Tsuyoshi Ueno, Atsushi Hori, Yutaro Kobori, Takahito Kai, Emiko Nishioka, Takehisa Matsukawa, Shintaro Makino, Takashi Miida
BACKGROUND: Insulin resistance (IR) is exacerbated during pregnancy via increases in insulin counterregulatory hormones. Maternal lipids are strong determinants of neonatal growth, although triglyceride-rich lipoproteins (TGRLs) cannot be transferred directly to the fetus through the placenta. The catabolism of TGRLs under physiological IR and the reduced synthesis of lipoprotein lipase (LPL) are poorly understood. We examined the association of maternal and umbilical cord blood (UCB)-LPL concentrations with maternal metabolic parameters and fetal development...
March 2, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36870521/analytical-evaluation-and-sigma-metrics-of-6-next-generation-chemistry-assays-on-the-abbott-architect-system
#18
JOURNAL ARTICLE
Annie Ren, Xiao Yan Wang, Pow Lee Cheng, Davor Brinc, Marvin I Berman, Vathany Kulasingam
BACKGROUND: We evaluated analytical and Sigma performance for 6 next generation chemistry assays on the Abbott Architect c8000 system. METHODS: Albumin with bromocresol purple or green, amylase, cholesterol, total protein, and urea nitrogen were analyzed using photometric technology. Analytical performance goals were defined based on Accreditation Canada Diagnostics (ACD) and Clinical Laboratory Improvement Amendments (CLIA). Precision study consisted of testing 2 quality control concentrations and 3patient serum sample pools, twice a day in quintuplicate over 5 days...
March 2, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36863694/a-novel-non-invasive-colorectal-cancer-diagnostic-method-volatile-organic-compounds-as-biomarkers
#19
JOURNAL ARTICLE
Miren Alustiza, Laura Ripoll, Antonio Canals, Oscar Murcia, Alejandro Martínez-Roca, Anabel García-Heredia, Mar Giner-Calabuig, Rodrigo Jover, Lorena Vidal
INTRODUCTION: Population-based fecal tests for colorectal cancer (CRC) screening have shown to reduce mortality thanks to the early detection of the disease. However, currently available fecal tests are limited in their sensitivity and specificity. Our aim is to look for volatile organic compounds in fecal samples as biomarkers for CRC detection. MATERIAL AND METHODS: Eighty participants were included; 24 had adenocarcinoma, 24 had adenomatous polyps and 32 presented no neoplasms...
February 28, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://read.qxmd.com/read/36858193/development-of-a-high-performance-liquid-chromatographic-glycated-albumin-assay-using-finger-prick-blood-samples
#20
JOURNAL ARTICLE
Masakazu Aihara, Tomoko Irie, Keiko Yasukawa, Itsushi Minoura, Noriko Miyauchi, Mitsumi Nishi, Norikazu Katayama, Kenji Yachiku, Hideaki Jinnouchi, Takashi Kadowaki, Toshimasa Yamauchi, Yutaka Yatomi, Naoto Kubota, Koshin Sekimizu
BACKGROUND: Home blood glucose monitoring can be effective for the self-management of diabetic patients. Hemoglobin A1c (HbA1c) is a widely used marker that reflects the average blood glucose within 1-2 months but does not sensitively respond to behavioral changes. Self-monitoring of blood glucose, continuous glucose monitoring, and flush glucose monitoring are sensitive; however, the cost and invasiveness of these tests prevent their widespread use. We focused on glycated albumin (GA), which reflects the average blood glucose levels over 1-2 weeks and established a GA measurement method for self-sampling, finger-prick blood, which may be submitted for testing through postal service to receive weekly results...
February 27, 2023: Clinica Chimica Acta; International Journal of Clinical Chemistry
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