Journals Clinical and Experimental Phar...

Clinical and Experimental Pharmacology & Physiology
Hongyun Zou, Lei Li, Zhilai Yang, Lili Tang, Chunhui Wang
Ischemia-reperfusion (I/R) injury is a common pathophysiological condition in ischemic stroke, involving various pathophysiological events, such as inflammation, cytotoxicity, neuronal loss and disruption of the blood-brain barrier (BBB). Rosavin is the major bioactive ingredient of Rhodiola Rosea L. with multiple therapeutic effects. The purpose of this was to investigate the role of rosavin in I/R-induced cerebral injury. A cell oxygen-glucose deprivation and reoxygenation (OGD/R) model and a mouse middle cerebral artery occlusion (MCAO) model were established to induce I/R injury in vitro and in vivo, respectively...
May 31, 2023: Clinical and Experimental Pharmacology & Physiology
Xiang Wei, Yang Lv, Chenxi Yang, Rifeng Gao, Su Zou, Yingjia Xu
Early inflammatory responses post myocardial infarction (MI) is associated with increased myocardial fibrosis and cardiac remodelling. The NLRP3 inflammasome, a key factor in this response, regulates the expression of interleukins (IL)-1β and IL-18. Inhibiting the inflammatory process may be beneficial for post-MI recovery. Bufalin effectively inhibits inflammation and fibrosis. The aim of this study was to evaluate the effects of bufalin and MCC950, an NLRP3 inflammasome inhibitor, as potential treatment agents for MI using an experimental mouse model...
May 27, 2023: Clinical and Experimental Pharmacology & Physiology
Guanyu Mu, Weiding Wang, Changle Liu, Juan Xie, Hao Zhang, Xiaowei Zhang, Jingjin Che, Gary Tse, Tong Liu, Guangping Li, Huaying Fu, Kangyin Chen
The diagnosis of heart failure with preserved ejection fraction (HFpEF) remains a challenge. There are three methods proposed as diagnostic tools. H2 FPEF score was determined by six weighted clinical characteristics and echocardiographic variables. Heart Failure Association (HFA)-PEFF algorithm consists of various functional and morphological variables as well as natriuretic peptides. SVI/S' is a novel echocardiographic parameter calculated by stroke volume index and mitral annulus systolic peak velocity. This study aimed to compare the three approaches in patients with suspected HFpEF...
May 19, 2023: Clinical and Experimental Pharmacology & Physiology
Hilal Üstündağ, Özlem Demir, Betül Çiçek, Mehmet Tahir Huyut, Neslihan Yüce, Taha Tavacı
This study investigated the synergistic protective effects of melatonin (MEL) and ascorbic acid (vitamin C, ASA) in treating sepsis-induced lung injury in rats. Rats were divided into five groups: control, cecal ligation and puncture (CLP), CLP + MEL, CLP + ASA and CLP + MEL + ASA. The effects of MEL (10 mg/kg), ASA (100 mg/kg) and their combination on oxidative stress, inflammation and histopathology were evaluated in septic rats' lung tissues...
May 18, 2023: Clinical and Experimental Pharmacology & Physiology
Gabriela Bielecka-Papierz, Ewa Poleszak, Joanna Listos, Bernadeta Szewczyk, Aleksandra Szopa, Jolanta Orzelska-Górka, Justyna Turek, Dorota Bederska-Łojewska, Małgorzata Jakóbczuk, Jarosław Szponar, Sylwia Talarek, Anna Serefko
Mephedrone is a representative of synthetic cathinones that is known from its rewarding and psychostimulant effects. It exerts behavioural sensitization after repeated and then interrupted administration. In our study, we investigated a role of the L-arginine-NO-cGMP-dependent signalling in the expression of sensitization to hyperlocomotion evoked by mephedrone. The study was carried out in male albino Swiss mice. The tested mice received mephedrone (2.5 mg/kg) for 5 consecutive days and on the 20th day of the experiment (the 'challenge' day) animals received both mephedrone (2...
May 17, 2023: Clinical and Experimental Pharmacology & Physiology
Vagisha Sharma, Sanghamitra Pattnaik, Himani Ahluwalia, Manpreet Kaur
Long coronavirus disease (COVID) is emerging as a common clinical entity in the current era. Autonomic dysfunction is one of the frequently reported post-COVID complications. We hypothesize a bi-directional relationship between the autonomic function and the COVID course. This postulation has been inadequately addressed in the literature. A retrospective cohort (pre and post-comparison) study was conducted on 30 young adults whose pre-COVID autonomic function test results were available. They were divided into case and control groups based on whether they tested reverse transcription polymerase chain reaction positive for COVID-19...
April 30, 2023: Clinical and Experimental Pharmacology & Physiology
Motohiro Tezuka, Kumi Tsuchioka, Kaoru Kobayashi, Yu Kuramochi, Sumiyoshi Kiguchi
Endometriosis is an oestrogen-dependent disease in which endometrial-like tissue grows outside the uterus in women of reproductive age. Accordingly, control of oestradiol (E2) levels is an effective treatment for endometriosis. Because gonadotropin-releasing hormone (GnRH) is the main controller of E2 secretion, control of GnRH signalling by GnRH antagonism is an effective strategy for the treatment of sex hormone-dependent diseases such as endometriosis. The purpose of the present study was to evaluate the effects of the potent, orally available and selective GnRH antagonist linzagolix on experimental endometriosis in rats and compare them with those of dienogest, which is used clinically to treat endometriosis...
April 26, 2023: Clinical and Experimental Pharmacology & Physiology
Anqi Li, Zhili Tan, Siyuan He, Haiqing Chu
Non-tuberculous mycobacterial infections are gradually increasing worldwide, with slow-growing mycobacteria such as Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium kansasii accounting for the majority of cases. The use of tetracyclines has received renewed attention in recent years, and this study was designed to investigate the antibacterial activity of omadacycline, eravacycline, tigecycline, sarecycline, minocycline and doxycycline against M. avium, M. intracellulare and M. kansasii. Susceptibility testing of six tetracyclines was conducted against M...
April 22, 2023: Clinical and Experimental Pharmacology & Physiology
Jian Zhou, Xuecheng Wang, Jinbo Yu, Yizhang Wu, Xiaorong Li, Xin Xie, XuMin Zhang, Dian Cheng, Bing Yang
OBJECTIVE: As a result of large, randomized trials and updates to clinical guidelines, antithrombotic therapy following percutaneous coronary intervention (PCI) has changed in recent years for patients with nonvalvular atrial fibrillation (NVAF). The purpose of this study was to investigate the real-world data of antithrombotic regimens at discharge and their evolving trends, as well as compare the effect of different therapies on the incidence of major cardiovascular and cerebrovascular ischemic events (MACCEs) and bleeding events in elderly patients...
April 14, 2023: Clinical and Experimental Pharmacology & Physiology
Songsong Jiang, Qian Li, Shiwen Han, Hengpeng Wang, Xinlei Tang, Tao Wang, Xiangren Meng
This study aimed to explore the anti-inflammatory effect of stachyose, a tetrasaccharide extracted from Stachys sieboldii Miq. A lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages model and a dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) BALB/C mice model was used to assess the anti-inflammatory effect of stachyose both in vitro and in vivo. The levels of Nitric oxide (NO) and cytokines (Interleukin-1β, Interleukin-6, Tumor necrosis factor-α) were detected using enzyme-linked immunosorbent assay (ELISA) methods; besides, hematoxylin-eosin (HE) staining was used to observe changes in intestinal morphology of mice...
March 28, 2023: Clinical and Experimental Pharmacology & Physiology
Yawei He, Wanjing Chen, Liang Yu, Yanwei Liu, Yunsheng Cheng, Xianghua Li, Gang Yu
Aerobic glycolysis is critical for the energy metabolism of cancer cells. This study focuses on the regulation of forkhead box A2 (FOXA2) on pyruvate kinase M2 (PKM2) and their effects on the glycolytic activity and malignant phenotype of thyroid carcinoma (THCA) cells. By analyzing four GEO datasets and querying bioinformatics systems, we obtained FOXA2 as a poorly expressed transcription factor in THCA. Later, we validated decreased FOXA2 mRNA and protein levels in THCA cells by qPCR and WB assays. FOXA2 upregulation in THCA cells suppressed the glucose uptake and lactate production, and it reduced the extracellular acidification rate (ECAR) but increased the oxygen consumption rate (OCR) of cells...
March 22, 2023: Clinical and Experimental Pharmacology & Physiology
Jue Wang, Pei Li, Lin Xin, Xiaogang Xu, Minggui Wang
We first reported the vanM vancomycin resistance gene in enterococci in Shanghai, China in 2006 and later found it to be the predominant van gene in vancomycin-resistant enterococci (VRE). In this study, we successively collected 1292 Enterococcus faecium and Enterococcus faecalis strains from in- and outpatients at Huashan Hospital, Fudan University, and found that nearly all of the isolates (1290/1292) were vancomycin-sensitive determined by the VITEK® 2 system. However, using a modified macromethod-based disk diffusion test, ten E...
March 20, 2023: Clinical and Experimental Pharmacology & Physiology
Xiaoqin Zhou, Chuntong Liu, Daolin Xia
Exposure to sevoflurane leads to serious neurological side effects, including neuronal apoptosis and cognitive impairment. In the mouse model, CDK9 was significantly downregulated after exposure to sevoflurane, but the effect of CDK9 on neuronal apoptosis and cognitive impairment after sevoflurane exposure has not been elucidated. Here, we found that the upregulation of P300 by sevoflurane in vitro and in vivo inhibited the expression of CDK9 and induced neuron apoptosis. The effect of sevoflurane on CDK9 expression is based on inhibition of its transcription process...
March 16, 2023: Clinical and Experimental Pharmacology & Physiology
Taijing Xu, Mengjun Liu, Zhao Xinhui, Shenglei Zhang, Shuchen Ma, Jun Wang, Yi Wang, Yide Guo, He Wang, Xuan Hong, Feng Liu
Many preclinical studies reported that Carboxyl terminus of Hsp70-interacting protein (CHIP) has cardiovascular protective effects. This study was designed to explore whether CHIP is related with cardiovascular disease (CVD) in maintanence hemodialysis (MHD) patients. 217 MHD patients and 150 healthy controls were recruited, serum CHIP concentration and clinical characteristics were measured. MHD patients were followed-up for 36 months and their cardiovascular events (CVEs) and survival conditions were recorded...
March 14, 2023: Clinical and Experimental Pharmacology & Physiology
Liping Liu, Langni Liu, Rui Liu, Jing Liu, Qian Cheng
The prognosis of multiple myeloma (MM) patients combined with renal insufficiency is poor. Renal fibrosis is an important pathological cause for MM patients combined with renal insufficiency. It is reported that epithelial-mesenchymal transition (EMT) of renal proximal tubular epithelial cells is an important mechanism in renal fibrosis. We speculated that EMT might play an important role in the renal insufficiency of MM with unclear mechanism. MM cells derived exosomes could affect the function of targeted cells by delivering miRNAs...
March 11, 2023: Clinical and Experimental Pharmacology & Physiology
Fangying Yang, Linkai Li, Yanhe Zhou, Wenxu Pan, Xinhua Liang, Ling Huang, Jing Huang, Yang Cheng, Lanlan Geng, Wanfu Xu, Sitang Gong
Rabeprazole is a representative of proton pump inhibitors and widely used in anti-ulcer treatment. However, the effect of Rabeprazole on gut barrier function remains to be identified. In this study, we show that ZO-1 expression is decreased in patients receiving Rabeprazole by immunofluorescence (IF) analysis. Western blotting (WB) and real-time PCR (qPCR) results demonstrate that Rabeprazole treatment leads to a significant downregulation of ZO-1 expression through inhibition of the FOXF1/STAT3 pathway, leading to destroy barrier function, which illustrates a novel pathway that Rabeprazole regulates barrier function in gastric epithelial cells...
March 10, 2023: Clinical and Experimental Pharmacology & Physiology
Ling Peng, Chunhu Zhang, Gong Xiao
Podocyte loss is a predictor of kidney disease development, including diabetic nephropathy. Astragalus polysaccharide (APS) was considered a renoprotective drug, while the mechanisms operated by APS on podocyte dysfunction are rarely mentioned. This study aims at the mechanistic underlying of APS on angiotensin II (Ang II)-induced podocyte dysfunction. Mouse glomerular podocytes MPC5 were induced with Ang II, the morphologic changes were observed, and Nephrin, Desmin, and WT-1 levels were determined. The MPC5 cells were treated with APS (50, 100, and 200 μg/mL) and transduced with retinoic acid receptor responder protein 1 (RARRES1) overexpression vectors...
March 6, 2023: Clinical and Experimental Pharmacology & Physiology
Fabrício Gomes da Silva, Márcia Helena Miranda Cardoso Podestá, Thayná Coelho Silva, Carlos Marcelo de Barros, Breno Fialho Vitarelli de Carvalho, Tiago Marques Dos Reis, Milena Carla Espósito, Danielle Aparecida Ferreira de Oliveira Marrafon, Denismar Alves Nogueira, Sudhir Diwan, Carla Speroni Ceron, Larissa Helena Torres
Postoperative pain is one of the main negative symptoms resulting from surgery and the use of new methods to control this symptom is of ever-increasing relevance. Opioid-sparing strategies, such as multimodal analgesia, are trends in this scenario. Pregabalin is a well-established treatment for neuropathic pain; however, it is still controversial in the surgical context for postoperative analgesia. This study investigated the effect of pregabalin on postoperative analgesia in patients undergoing abdominal hysterectomy...
March 2023: Clinical and Experimental Pharmacology & Physiology
Surbhi Tyagi, Asha Tyagi, Salhotra Rashmi, Medha Mohta, Bindiya Gupta
Postpartum haemorrhage remains a significant cause of maternal morbidity and mortality with the commonest reason being uterine atony. For prevention of uterine atony during caesarean delivery, oxytocin is advocated as a first line drug. There is however no published data regarding utility of a weight-based oxytocin infusion. The present study evaluated dose-response relationship for oxytocin infusion when used as weight-based regimen. A total of 55 non-laboring patients without risk factors for uterine atony and scheduled for caesarean delivery under spinal anaesthesia were enrolled...
February 27, 2023: Clinical and Experimental Pharmacology & Physiology
Joanna Sobiak, Paweł Żero, Jacek Zachwieja, Danuta Ostalska-Nowicka, Tomasz Pawiński
BACKGROUND: In pediatric patients, there is no data on the recommended area under the concentration-time curve from 0 to 12 h (AUC0-12 ) for free MPA (fMPA), which is the active form of the drug, responsible for the pharmacological effect. We decided to establish the limited sampling strategy (LSS) for fMPA for its use in MPA therapeutic monitoring in children with nephrotic syndrome treated with mycophenolate mofetil (MMF). METHODS: This study included 23 children (aged 11±4 years) from whom eight blood samples were collected within 12 h after MMF administration...
February 27, 2023: Clinical and Experimental Pharmacology & Physiology
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