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Devang Sharma, Jacqueline Zillioux, Iyad Khourdaji, Katy Reines, Karen Wheeler, Raymond Costabile, Parviz Kavoussi, Ryan Smith
Clomiphene citrate (CC) is commonly used off-label for the treatment of male infertility, yet there is limited data to guide patient selection. To identify a subset of patients more likely to benefit from CC, we aimed to define predictors of improvement in semen parameters among men receiving CC. We retrospectively analysed 151 men treated with at least 25 mg CC daily for male infertility and/or hypogonadism at two institutions between 2004 and 2014. Men previously on testosterone were excluded. The primary outcome was change in semen parameters...
February 18, 2019: Andrologia
Garrett K Berger, Luriel I Smith-Harrison, Jay I Sandlow
Agglutination is a finding noted in semen analyses (SAs) that often causes confusion as to its significance. While some have attributed agglutination to antisperm antibodies (ASAs), there are other causes as well, such as genital tract infection and ascorbic acid deficiency. Additionally, it is known that patients with ASAs often have risk factors such as a history of scrotal trauma or surgery. Therefore, we sought to determine the prevalence of agglutination in our patient population and correlate it with these risk factors, regardless of the presence/absence of ASAs...
February 13, 2019: Andrologia
Hatef Talebi, Mohammad Reza Farahpour
This study is aimed to analyse the cross-link between cyclin D1, cdk-4, p21, PCNA and DNA damage during different periods of reperfusion following experimental torsion in rats. Thirty mature male Wistar rats (N = 6) were used. Following 4 hr from torsion induction, the reperfusion was induced. Animals were subdivided into groups, including 4 hr torsion-induced (T1), 1 hr post-reperfusion (T2), 2 hr post-reperfusion (T3), 4 hr post-reperfusion (T4) and 8 hr post-reperfusion (T5) groups. The seminiferous tubules differentiation (TDI) and spermatogenesis indices were evaluated...
February 11, 2019: Andrologia
Maryam Salem, Tooba Mirzapour, Abolfazl Bayrami, Mohsen Sagha
Bone marrow mesenchymal stem cells (BM-MSCs) were first cultured under induction of retinoic acid (RA), Sertoli cells conditioned medium and RA + con (conditioned medium) as treatment groups. The presence of Sertoli cells was confirmed by immunocytochemistry of follicle-stimulating hormone receptor in Sertoli cells and flow cytometry by anti-Gata4 antibody. Cell viability and morphology of nucleus and cytoplasm of BM-MSCs were evaluated by MTT test and DAPI staining respectively. The expression of Oct4, Plzf, Scp3, Caspases 8, 9 and 3 genes was evaluated by RT-PCR...
February 11, 2019: Andrologia
Levent Simsek, Ayse Gul Zamani, Hakan Hakkı Taskapu, Mahmut Selman Yildirim
BACKGROUND: Klinefelter syndrome(KS), affecting 1 in 500-1,000 newborn males, is the most common sex chromosome aneuploidy among males with primary hypogonadism. Isochromosome Xq on the other hand is a rare variant of Klinefelter syndrome, accounting approximately 0.3% of all KS and associated with normal height and androgenisation compared to classical KS. Here, we present a case of isochromosome Xq variant of KS with similar clinical and cytogenetic findings with the few cases reported before...
February 11, 2019: Andrologia
Masoomeh Mohammadzadeh, Vahid Ramazani, Mohammad Ali Khalili, Hamed Hamishekar, Laleh Dehghan Marvast, Ali Reza Talebi, Hadi Zare-Zardini, Saeed Ghasemi
The aim of this study was to comprise the effect of catalase on sperm parameters and chromatin in normospermic persons. Semen samples were obtained from fertile men. A certain amount of different concentrations of catalase (0.1, 1, 10, 50, 100, 150 and 200 was added to each vial containing semen. Control group had similar condition to treated groups without treatment. Treatment was done for one hour in incubator and 4 and 24 hr in room temperature. Sperm parameters (motility, viability and morphology) and chromatin were evaluated after incubation...
February 11, 2019: Andrologia
Guangmin Yang, Mehmutjan Muzepper
Elevated platelet levels have been postulated to be associated with cardiovascular diseases, conditions closely linked to erectile dysfunction (ED). The current systematic review and meta-analysis was performed to assess the platelet indices, which including platelet count (PLT), mean platelet volume (MPV) and platelet distribution width (PDW) in subjects with ED compared to controls in an attempt to clarify the possible role of platelet indices in the pathogenesis of ED. We initially screened the candidate studies observing the possible association between platelet indices and ED following literature search of database Cochrane Library, PubMed, EMBASE and MEDLINE and therefore included the studies based on the pre-defined inclusion and exclusion criteria...
February 10, 2019: Andrologia
Yang Guo, Jin-Ming Li, Yu Xiang, Yang-You Li, Jing Huang, Xian-Zhong Deng, Zheng-Wei Yang
There is a scarcity of morphometric data on the developmental and ageing changes in the epididymis and seminal vesicle in young and old rats. Eighty-six normal male Sprague-Dawley rats were randomly sampled from a cohort of animals aged 1-36 months (7-9 animals each age group). The epididymis and seminal vesicle (with the closely attached coagulating gland) were removed, and methacrylate resin-embedded sections were prepared for quantitative study of key histological structures by light microscopy. Stereological methods (point counting and optical disector) were used to estimate the total volumes of sperm mass, secretion (glandular lumen) and other structures and the number of spermatozoa...
February 7, 2019: Andrologia
Zhangren Yan, Chunhua Huang, Gang Huang, Yunbo Wu, Jiangang Wang, Jun Yi, Wenli Mao, Wanchun Wang
This study aimed to investigate the mechanism of Jiedu Huoxue decoction (JDHXD) in type III prostatitis based on the NF-κB signalling pathway. Twenty-six Sprague-Dawley male rats were divided into blank control, model, positive (Prostate Plus), low-dose JDHXD, medium-dose JDHXD and high-dose JDHXD groups. Type III prostatitis rat model was established and confirmed with HE staining. NF-кB P50 and NF-κB P65 expression was detected with immunohistochemistry. NF-κB mRNA expression was detected with qRT-PCR...
February 6, 2019: Andrologia
Tayebe Faraji, Hamid Reza Momeni, Mahdi Malmir
Cadmium is known as an oxidative stress-inducing factor. Silymarin extracted from Silybum marianum is regarded as a potent antioxidant. The present study investigated the preventing effects of silymarin on cadmium chloride-induced toxicity in terms of testis histopathology and serum testosterone level as well as oxidative stress indicators in mice. In addition, the activities of antioxidant defence enzymes was evaluated. Adult male mice were divided into four groups (n = 6 in each group): (a) control; (b) cadmium chloride; (c) silymarin + cadmium chloride and (d) Silymarin...
February 6, 2019: Andrologia
Yawei Xu, Yuanyuan Zhang, Yongjiao Yang, Xiaoqiang Liu, Yegang Chen
To evaluate the value of seminal plasma miR-210-3p as a novel and non-invasive biomarker for screening dyszoospermia caused by varicocele. Semen samples from patients with varicocele and healthy males were collected for semen analysis and quantitative real-time polymerase chain reaction. Cox univariate and multivariate analysis and receiver operating characteristic curve analysis were used to assess the relationship between the level of seminal plasma miR-210-3p and impaired spermatogenic function. Our results showed that the level of seminal plasma miR-210-3p in the varicocele patients was 2...
February 3, 2019: Andrologia
Afreen Aftab, Thankam R Varma, Bibhas Kar
Presence of chromosomal anomalies is well known to be associated with reproductive failures where the incidence of chromosomal translocations is higher. Y:1 chromosomal translocations are reported to be rare and may have variable phenotypic effects such as infertility amongst others. The patient presented with azoospermia and dyslipidemia and coronary arterial disease. Cytogenetic tests such as karyotyping revealed the translocation, and fluorescent in situ hybridisation was performed to investigate the presence or absence of SRY gene...
February 3, 2019: Andrologia
Mikito Tanaka, Koji Chiba, Keisuke Okada, Masato Fujisawa
Mirabegron is a selective beta3-adrenoceptor (β3 -AR) agonist, which is commonly used for the treatment of overactive bladder. This medicine is associated with atrophy of reproductive organs in rats. However, no study has examined the detailed action and mechanism of its toxicity in reproductive cells. In this study, we examined the effect of mirabegron on primary cultured rat Sertoli cells. Firstly, RT-PCR and immunocytochemistry revealed that β3 -AR was present in rat Sertoli cells. Then, primary cultured rat Sertoli cells were treated with mirabegron...
January 31, 2019: Andrologia
Mahsa Hallaj Salahipour, Shapour Hasanzadeh, Hassan Malekinejad, Mazdak Razi, Farhad Farrokhi-Ardebili
This study was performed to investigate in vitro effects of deoxynivalenol (DON) on mice sperm quality parameters including viability, motility and DNA damages at various concentrations and exposure times. Mice spermatozoa were exposed to DON at 0, 2.5, 5 and 10 µM for 1, 3 and 6 hr, motility parameters were evaluated by computer-assisted analysis and viability was examined by colorimetric metabolic activity assay and HOS test. DNA damage was examined by acridine orange staining, and sperm damages via lipid peroxidation pathway were determined by malondialdehyde (MDA) content measurement...
January 31, 2019: Andrologia
Enis Yilmaz, Ecem Kaya-Sezginer, Didem Yilmaz-Oral, Tugba Cengiz, Nur Bayatli, Serap Gur
Men with hypertension often develop erectile dysfunction (ED). The present study aimed to examine the effects of sodium hydrosulphide (NaHS), a hydrogen (H2 S) donor, treatment on ED in nitric oxide synthase (NOS) inhibitor (L-NAME)-induced hypertensive rats. Forty adult Sprague-Dawley rats were divided into four groups: control, NaHS (0.037 mg kg day-1 )-treated control, L-NAME-induced hypertension (40 mg kg day-1 ) and NaHS-treated L-NAME-induced hypertension. The ratio of intracavernosal pressure to mean arterial pressure and isometric tension of corpus cavernosum (CC) were measured...
January 31, 2019: Andrologia
Gui-Chao Dai, Yu Meng, Li-Kun Zhang, Ye-Qing Du, Fei Wen, Tian-Yu Feng, Jian-Hong Hu
Adding a certain amount of antioxidants to semen extender has been shown to improve semen quality. The aim of present study was to elucidate whether the supplementation of melatonin to the Tris-based extender (CTR) could enhance the quality of ram spermatozoa during storage at 4°C. Ram semen samples were collected and diluted with CTR extender containing different concentrations (0, 0.05 (M 0.05), 0.1 (M 0.1), 0.2 (M 0.2) or 0.4 (M 0.4) mM) of melatonin. Sperm routine indicators, mitochondrial activity, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content were analysed in control and melatonin treatment groups...
January 29, 2019: Andrologia
Mustafa Suat Bolat, Fatih Kocamanoglu, Murat Gulsen, Mesut Sengul, Ramazan Asci
Impacts of a subinguinal microsurgical varicocelectomy on the sperm parameters and fertility rates were investigated in three different ages according to their age at the time of the varicocelectomy: Group 1 was ≤20 years old, Group 2 was 21-30 years old, and Group 3 was ≥31 years old. The patients were also classified both preoperatively and post-operatively according to the total motile sperm count (TMSC) into the following categories: invitro fertilisation, intrauterine insemination and naturally fertile...
January 28, 2019: Andrologia
Jing-Ping Li, Xin-Zong Zhang, Jing-Gen Wu, Zhong-Yan Liang, Yong-Hong Tian, Chong Chen, Yun-Ge Tang, Feng-Bin Zhang
Microsurgical vasoepididymostomy (MVE) is recommended as a first-line option for treatment of epididymal obstructive azoospermia (EOA). However, early indicators for predicting patency and natural pregnancy are unclear. Our aim was to explore the early predictive value of seminal plasma neutral alpha-glucosidase (NAG) activity for patency and natural pregnancy after MVE. Eighty-four patients with EOA who underwent MVE were enrolled in this study. The post-operative patency and natural pregnancy rates were 60...
January 28, 2019: Andrologia
Wuhua Ni, Chengshuang Pan, Qiongqiong Pan, Qianjin Fei, Xuefeng Huang, Chunni Zhang
Abnormal imprinted genes methylation in spermatozoa has been shown to be associated with subfertility. However, the relationship between sperm DNA damage and specific imprinted genes methylation remains unclear. In this study, DNA methylation levels were determined at seven imprinted genes loci (H19, INS-IGF2, KCNQ1, MEG3, MEST, PEG3 and SNRPN) in 66 semen samples using the MSRE-qPCR method. The semen samples were divided into two groups according to the threshold value (25%) of DNA fragmentation index (DFI)...
January 24, 2019: Andrologia
Felipe Pezo, Fabiola Zambrano, Pamela Uribe, Alfredo Ramírez-Reveco, Fernando Romero, Raúl Sanchéz
Pre-treatment of boar semen with a red light photostimulation procedure increases its "in vivo" fertilising ability. However, "in vitro" conducted studies shown contradictory results regarding the ability of photostimulated spermatozoa to react against strong stress and to achieve the capacitation status. The aim here was to determine the effect of photostimulation on the response to short-term moderate thermal stress of boar semen. Boar semen was exposed to red LED light regime emitting a 620-630 nm during 10 min of light, 10 min of rest and 10 min of light after 3 hr since semen was collected...
January 18, 2019: Andrologia
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