journal
https://read.qxmd.com/read/38521006/effect-of-estrogen-and-progesterone-on-intracellular-free-zinc-and-zinc-transporter-expression-in-bovine-oviduct-epithelial-cells
#1
JOURNAL ARTICLE
Ana Malen Pascua, Gisela Barbisan, Noelia Nikoloff, Ana Cristina Carranza-Martín, Mariana Carolina Fabra, Juan Patricio Anchordoquy, Marianela Balbi, Mauricio Javier Giuliodori, Cecilia Cristina Furnus, Juan Mateo Anchordoquy
Zinc (Zn) plays essential roles in numerous cellular processes. However, there is limited understanding of Zn homeostasis within the bovine reproductive system. This study investigated the influence of estradiol (E2 ) and progesterone (P4 ) on Zn transporter expression and intracellular free Zn levels in bovine oviduct epithelial cells (BOEC). For this purpose, cells were harvested from slaughtered cows and cultured in vitro. Intracellular Zn concentrations were measured using FluoZin-3AM staining, while real-time polymerase chain reaction assessed Zn transporter gene expression and quantification...
March 19, 2024: Theriogenology
https://read.qxmd.com/read/38521007/metabolites-assay-offers-potential-solution-to-improve-the-rooster-semen-cryopreservation
#2
JOURNAL ARTICLE
Xue Du, Yuanning Zhang, Duoxi Li, Jie Han, Yali Liu, Lijuan Bai, Tao Huang, Ming Cui, Panlin Wang, Xianzhong Zheng, Ayong Zhao
Semen cryopreservation represents a promising technology utilized for preserving high-quality chicken varieties in husbandry practices. However, the efficacy of this methodology is significantly impeded by the diminished quality of sperm. Metabolites, as the end products of metabolic reactions, serve as indicators of biological processes and offer insights into physiological conditions. In this study, we investigaged the sperm quality and alteration in metabolic profiles during the cryopreservation of Longyou Partridge Chicken semen...
March 16, 2024: Theriogenology
https://read.qxmd.com/read/38518659/a-single-administration-of-carbetocin-before-electroejaculation-increases-the-insemination-doses-produced-from-each-ejaculate-in-rams
#3
JOURNAL ARTICLE
J C Orihuela, A Freitas-de-Melo, L Pinto-Santini, J Giriboni, M N Viera, J Santiago-Moreno, F Beracochea, P Silveira, R Ungerfeld
The aim of the study was to determine the effect of carbetocin administration (a long-acting analog of oxytocin) 20 or 10 min before electroejaculation (EE) on the duration of semen collection procedure, quantitative and qualitative characteristics of the ejaculate, and stress biomarkers in rams. Semen was collected from 12 Corriedale rams (age, 2.5-5.5 years old) with EE, in a Latin-square design, administrating carbetocin (0.2 mg/100 kg of body weight i.v.) 20 or 10 min before EE, or without carbetocin administration (CB-20, CB-10, and CON treatments, respectively)...
March 15, 2024: Theriogenology
https://read.qxmd.com/read/38507824/enhancement-of-progesterone-biosynthesis-via-kisspeptin-stimulation-upregulation-of-steroidogenic-transcripts-and-phosphorylated-extracellular-signal-regulated-kinase-p-erk1-2-expression-in-the-buffalo-luteal-cells
#4
JOURNAL ARTICLE
M P Thejaswini, M K Patra, R Sharma, Md R A Raza, J K Sasidharan, M Karikalan, Z B Dubal, S K Ghosh, G K Gaur, S K Singh, N Krishnaswamy
The presence of Kisspeptin (Kp) and its receptors in the corpus luteum (CL) of buffalo has recently been demonstrated. In this study, we investigated the role of Kp in the modulation of progesterone (P4 ) synthesis in vitro. The primary culture of bubaline luteal cells (LCs) was treated with 10, 50, and 100 nM of Kp and Kp antagonist (KpA) alongside a vehicle control. The combined effect of Kp and KpA was assessed at 100 nM concentration. Intracellular response to Kp treatment in the LCs was assessed by examining transcript profiles (LHR, STAR, CYP11A1, HSD3B1, and ERK1/2) using quantitative polymerase chain reaction (qPCR)...
March 14, 2024: Theriogenology
https://read.qxmd.com/read/38503100/heme-oxygenase-1-ho1-regulates-autophagy-and-apoptosis-via-the-pi3k-akt-mtor-signaling-pathway-of-yak-sertoli-cells
#5
JOURNAL ARTICLE
Qiu Yan, Qi Wang, Jinghong Nan, Tingting Chen, Juntao Wang, Yong Zhang, Ligang Yuan
Successful male reproduction depends on healthy testes. Autophagy has been confirmed to be active during many cellular events associated with the testes. It is not only crucial for testicular spermatogenesis but is also an essential regulatory mechanism for Sertoli cell (SCs) ectoplasmic specialization integrity and normal function of the blood-testis-barrier. Hypoxic stress induces oxidative damage, apoptosis, and autophagy, negatively affecting the male reproductive system. Cryptorchidism is a common condition associated with infertility...
March 13, 2024: Theriogenology
https://read.qxmd.com/read/38490113/role-and-action-mechanisms-of-mir-149-and-mir-31-in-regulating-function-of-pig-cumulus-cells-and-oocytes
#6
JOURNAL ARTICLE
Jia-Shun Wu, Shuai Gong, Min Zhang, Rui-Jie Ma, Hui-Li Wang, Ming-Jiu Luo, Nan He, Jing-He Tan
Understanding the mechanisms for oocyte maturation and optimizing the protocols for in vitro maturation (IVM) are greatly important for improving developmental potential of IVM oocytes. The miRNAs expressed in cumulus cells (CCs) play important roles in oocyte maturation and may be used as markers for selection of competent oocytes/embryos. Although a recent study from our group identified several new CCs-expressed miRNAs that regulate cumulus expansion (CE) and CC apoptosis (CCA) in mouse oocytes, validation of these findings and further investigation of mechanisms of action in other model species was essential before wider applications...
March 11, 2024: Theriogenology
https://read.qxmd.com/read/38490112/optimizing-rebreed21-i-evaluation-of-endocrine-and-ovarian-dynamics-in-non-bred-bos-indicus-heifers
#7
JOURNAL ARTICLE
João Paulo N Andrade, Rafael R Domingues, Bruno P Carvalho, Victor Gomez-Leon, Alexandre B Prata, Roberto Sartori, Milo C Wiltbank
The present study evaluated follicular and endocrine dynamics during ReBreed21, a reproductive strategy that allows resynchronization of ovulation every 21 days in Bos indicus (Nelore) heifers. A synchronized estrous cycle was induced using a standard timed ovulation protocol (d -10: P4 implant inserted + 2 mg estradiol benzoate; d -2: P4 removed+ 0.5 mg cloprostenol + 0.6 mg estradiol cypionate + 200 IU equine chorionic gonadotropin (eCG); d0: 8.4 μg buserelin) without AI to ensure nonpregnancy in heifers...
March 8, 2024: Theriogenology
https://read.qxmd.com/read/38479090/characterization-of-metabolic-patterns-in-porcine-cumulus-cells-during-meiotic-maturation
#8
JOURNAL ARTICLE
Ming Gao, Hengjie Wang, Minjian Chen, Shuai Zhu, Yongfu He, Qiang Wang, Ling Gu
Metabolic coupling between oocytes and the surrounding somatic cells allows for normal two-way communication, and their interactions is necessary for generating developmentally competent eggs. However, the metabolic framework that support oocyte maturation in surrounding cumulus cells is still lacking. Herin, we established a temporal metabolome profile of porcine cumulus cells at three key stages during oocyte maturation, illustrating the picture of global metabolic network in cumulus cells. Importantly, we discovered the novel metabolic signature in cumulus cells during meiotic maturation, in specific, significant consumption of fatty acids, elevated activity of hexosamine biosynthetic pathway (HBP), and enhanced polyamine biosynthesis...
March 7, 2024: Theriogenology
https://read.qxmd.com/read/38471389/estrogens-improve-the-pregnancy-rate-in-cattle-a-review-and-meta-analysis
#9
REVIEW
Ebrahim Taghizadeh, Farid Barati, Aziz A Fallah, Maryam Hemmatzadeh-Dastgerdi, Mohammad-Saleh Nejabati
Estrogens have proven to be effective in bovine estrus induction protocols. Considering the extensive use of these products in large-scale estrus synchronization, the primary objective of the present study was to assess their effects on pregnancy rate (PR) using a meta-analysis approach. A total of 797 papers were screened from three major databases (PubMed, Web of Science, Scopus). Sixty-one studies were eligible for inclusion in the meta-analysis. The pregnancy status (success or failure) at 30 days post-insemination was considered as the effect size data...
March 7, 2024: Theriogenology
https://read.qxmd.com/read/38460201/schisandrin-b-enhances-embryo-competence-and-potentially-mitigates-endoplasmic-reticulum-stress-during-porcine-preimplantation-development
#10
JOURNAL ARTICLE
Chuang Li, Kuk Bin Ji, Ho Yong Choi, Haixing Liu, Minkyu Kim
Endoplasmic reticulum (ER) stress induced by agents such as tunicamycin (TM) substantially impedes the developmental progression of porcine embryos. Lignan compounds such as Schisandrin B (Sch-B), may have the potential to mitigate this stress. However, there are few studies on the effects of Sch-B on embryo development. To address this research gap, this study evaluates the protective efficacy of Sch-B against TM-induced ER stress during pivotal stages of porcine embryogenesis. Notably, embryos treated with Sch-B exhibited pronounced resistance to TM-induced developmental arrest, particularly at the 4-cell stage, facilitating progression to the 8-cell stage and subsequent blastocyst formation...
March 5, 2024: Theriogenology
https://read.qxmd.com/read/38457855/developmental-programming-of-production-and-reproduction-in-dairy-cows-iv-association-of-maternal-milk-fat-and-protein-percentage-and-milk-fat-to-protein-ratio-with-offspring-s-birth-weight-survival-productive-and-reproductive-performance-and-amh-concentration
#11
JOURNAL ARTICLE
Emadeddin Mobedi, Hamid Reza Dehghan Harati, Iman Allahyari, Faramarz Gharagozlou, Mehdi Vojgani, Reza Hemmati Baghbanani, Amirsina Akbarinejad, Vahid Akbarinejad
Although the association of maternal milk production with developmental programming of offspring has been investigated, there is limited information available on the relationship of maternal milk components with productive and reproductive performance of the offspring. Therefore, the present study was conducted to analyze the association of maternal milk fat and protein percentage and milk fat to protein ratio with birth weight, survival, productive and reproductive performance and AMH concentration in the offspring...
March 2, 2024: Theriogenology
https://read.qxmd.com/read/38484673/expression-of-izumo1-and-juno-in-the-gonads-of-domestic-cats-felis-catus
#12
JOURNAL ARTICLE
Phakjira Sanguansook, Watchareewan Rodprasert, Chenphop Sawangmake, Lourdes Gimeno, José Luis Ferran, Pilar Sòria-Monzó, Manuel Avilés, Mª José Izquierdo Rico, Kaywalee Chatdarong
Because of the time-consuming nature of surgical neutering and the rapid rate of reproduction among domestic cats, it is crucial to investigate alternative, nonsurgical methods of contraception for this species. Sperm protein IZUMO1 and its oocyte receptor JUNO have been proposed as potential targets for nonsurgical contraceptives. This study aimed to demonstrate (1) the protein coding sequence of feline IZUMO1 and JUNO, (2) gene expression in specific organs by measuring mRNA levels in different visceral tissues, and (3) the expression of IZUMO1 and JUNO during sperm maturation and folliculogenesis, respectively...
March 1, 2024: Theriogenology
https://read.qxmd.com/read/38457854/neddylation-inhibition-affects-early-embryonic-development-by-disrupting-maternal-to-zygotic-transition-and-mitochondrial-function-in-mice
#13
JOURNAL ARTICLE
Mingxiao Liu, Zhiming Ding, Peihao Sun, Shuo Zhou, Hanxiao Wu, Lijun Huo, Liguo Yang, John S Davis, Aixin Liang
Post-translational modifications (PTMs) are critical for early development in mice because early cleavage-stage embryos are characterized by transcriptional inactivity. Neddylation is an important ubiquitin-like PTM that regulates multiple biophysical processes. However, the exact roles of neddylation in regulating early embryonic development remain largely unknown. In the present study, we found that inhibition of neddylation by specific inhibitor MLN4924 led to severe arrest of early embryonic development...
February 28, 2024: Theriogenology
https://read.qxmd.com/read/38430798/target-silencing-of-porcine-spag6-and-ppp1cc-by-shrna-attenuated-sperm-motility
#14
JOURNAL ARTICLE
Hongyan Ren, Yandi Zhang, Yanzhen Bi, Heng Wang, Guijie Fang, Pengxiang Zhao
The quality of sperm significantly influences the reproductive efficiency of pig herds. High-quality sperm is necessary for efficient fertilization and to maximize the litter numbers in commercial pig farming. However, the understanding of genes regulating porcine sperm motility and viability is limited. In this study, we validated porcine sperm/Sertoli-specific promoters through the luciferase reporter system and identified vital genes for sperm quality via loss-of-function means. Further, the shRNAs driven by the ACE and SP-10 promoters were used to knockdown the SPAG6 and PPP1CC genes which were provisionally important for sperm quality...
February 28, 2024: Theriogenology
https://read.qxmd.com/read/38471390/comparison-of-icsi-ivf-and-in-vivo-derived-embryos-to-produce-crispr-cas9-gene-edited-pigs-for-xenotransplantation
#15
JOURNAL ARTICLE
Olinda Briski, Gastón Emilio La Motta, Laura Daniela Ratner, Federico Andrés Allegroni, Santiago Pillado, Guadalupe Álvarez, Betiana Gutierrez, Lisa Tarragona, Andrea Zaccagnini, Marcelo Acerbo, Carla Ciampi, Rafael Fernández-Martin, Daniel Felipe Salamone
Genome editing in pigs for xenotransplantation has seen significant advances in recent years. This study compared three methodologies to generate gene-edited embryos, including co-injection of sperm together with the CRISPR-Cas9 system into oocytes, named ICSI-MGE (mediated gene editing); microinjection of CRISPR-Cas9 components into oocytes followed by in vitro fertilization (IVF), and microinjection of in vivo fertilized zygotes with the CRISPR-Cas9 system. Our goal was to knock-out (KO) porcine genes involved in the biosynthesis of xenoantigens responsible for the hyperacute rejection of interspecific xenografts, namely GGTA1, CMAH, and β4GalNT2...
February 27, 2024: Theriogenology
https://read.qxmd.com/read/38432143/characterizing-the-relationship-between-gonadotropin-releasing-hormone-gnrh-kisspeptin-and-rfamide-related-peptide-3-rfrp-3-neurons-in-the-equine-hypothalamus-across-the-estrous-cycle-and-in-the-anovulatory-seasons
#16
JOURNAL ARTICLE
B M McGrath, S T Norman, C A Gaspardis, J L Rose, C J Scott
To understand better the role that kisspeptin plays in regulating seasonal and estrous cycle changes in the mare, this study investigated the number, location and interactions between GnRH, kisspeptin and RFRP-3 neurons in the equine hypothalamus. Hypothalami were collected from mares during the non-breeding season, vernal transition and various stages of the breeding season. Fluorescent immunohistochemistry was used to label the neuropeptides of interest. GnRH cells were observed primarily in the arcuate nucleus (ARC), while very few labeled cells were identified in the pre-optic area (POA)...
February 27, 2024: Theriogenology
https://read.qxmd.com/read/38428333/sperm-binding-to-oviduct-epithelial-spheroids-varies-among-males-and-ejaculates-but-not-among-females-in-pigs
#17
JOURNAL ARTICLE
Lorraine Schmaltz, Théo Prudhomme, Guillaume Tsikis, Karine Reynaud, Isabelle Mérour, Pascal Mermillod, Marie Saint-Dizier
The elimination of ejaculates and males with low fertility despite good sperm motility and morphology is crucial to maintain high pregnancy rates after artificial insemination (AI) in farm animals. The ability of sperm to survive in the female tract is particularly crucial in pigs due to the large variation in the timing between AI and ovulation and the high number of oocytes to fertilise. The objective of this study was to characterise a new in vitro model of oviduct sperm reservoir using porcine oviduct epithelial spheroids (OES) and to assess the variability in sperm binding to OES among gilts, boars and their ejaculates...
February 27, 2024: Theriogenology
https://read.qxmd.com/read/38437767/seminal-extracellular-vesicles-alter-porcine-in-vitro-fertilization-outcome-by-modulating-sperm-metabolism
#18
JOURNAL ARTICLE
Isabel Barranco, Marcella Spinaci, Salvatore Nesci, Yentel Mateo-Otero, Vito Antonio Baldassarro, Cristina Algieri, Diego Bucci, Jordi Roca
Porcine seminal plasma (SP) is loaded with a heterogeneous population of extracellular vesicles (sEVs) that modulate several reproductive-related processes. This study investigated the effect of two sEV subsets, small (S-sEVs) and large (L-sEVs), on porcine in vitro fertilization (IVF). The sEVs were isolated from nine SP pools (five ejaculates/pool) using a size-exclusion chromatography-based procedure and characterized for quantity (total protein), morphology (cryogenic electron microscopy), size distribution (dynamic light scattering), purity and EV-protein markers (flow cytometry; albumin, CD81, HSP90β)...
February 26, 2024: Theriogenology
https://read.qxmd.com/read/38428334/meta-analysis-to-determine-efficacy-of-colony-stimulating-factor-2-for-improving-pregnancy-success-after-embryo-transfer-in-cattle
#19
JOURNAL ARTICLE
Peter J Hansen, Eliab Estrada-Cortés, Thiago F Amaral, Rosabel Ramírez-Hernández
Results have been inconsistent as to whether addition of colony stimulating factor 2 (CSF2) to culture medium improves embryo competence for establishment of pregnancy in cattle and humans. The purpose of the current study was to use all available experiments in cattle concerning effects of CSF2 on pregnancy success after transfer into recipient cattle. The approach was to perform a meta-analysis of all published data sets as well as data from an unpublished experiment described for the first time here. Meta-analysis failed to support the hypothesis that addition of CSF2 to embryo culture medium improves competence of bovine blastocysts to increase pregnancy or calving rates after transfer into recipient females...
February 23, 2024: Theriogenology
https://read.qxmd.com/read/38422566/circular-rna-circrps19-promotes-chicken-granulosa-cell-proliferation-and-steroid-hormone-synthesis-by-interrupting-the-mir-218-5p-inhbb-axis
#20
JOURNAL ARTICLE
Yuanhang Wei, Xiaoxu Shen, Xiyu Zhao, Haorong He, Yao Zhang, Qing Zhu, Huadong Yin
Ovarian follicle development is an important physiological activity for females and makes great significance in maintaining female health and reproduction performance. The development of ovarian follicle is mainly affected by the granulosa cells (GCs), whose growth is regulated by a variety of factors. Here, we identified a novel circular RNA (circRNA) derived from the Ribosomal protein S19 (RPS19) gene, named circRPS19, which is differentially expressed during chicken ovarian follicle development. Further explorations identified that circRPS19 promotes GCs proliferation and steroid hormone synthesis...
February 23, 2024: Theriogenology
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