Olinda Briski, Gastón Emilio La Motta, Laura Daniela Ratner, Federico Andrés Allegroni, Santiago Pillado, Guadalupe Álvarez, Betiana Gutierrez, Lisa Tarragona, Andrea Zaccagnini, Marcelo Acerbo, Carla Ciampi, Rafael Fernández-Martin, Daniel Felipe Salamone
Genome editing in pigs for xenotransplantation has seen significant advances in recent years. This study compared three methodologies to generate gene-edited embryos, including co-injection of sperm together with the CRISPR-Cas9 system into oocytes, named ICSI-MGE (mediated gene editing); microinjection of CRISPR-Cas9 components into oocytes followed by in vitro fertilization (IVF), and microinjection of in vivo fertilized zygotes with the CRISPR-Cas9 system. Our goal was to knock-out (KO) porcine genes involved in the biosynthesis of xenoantigens responsible for the hyperacute rejection of interspecific xenografts, namely GGTA1, CMAH, and β4GalNT2...
February 27, 2024: Theriogenology