Reo Matsuzaka, Hiroki Yamaguchi, Chiharu Ohira, Tomoe Kurita, Naoki Iwashita, Yoshiichi Takagi, Tomomi Nishino, Kyoko Noda, Kazutoshi Sugita, Masayo Kushiro, Shiro Miyake, Tomoki Fukuyama
This study investigated the immunotoxic effects of the mycotoxin nivalenol (NIV) using antigen-presenting cells and a mouse model of atopic dermatitis (AD). In vitro experiments were conducted using a mouse macrophage cell line (RAW 264.7) and mouse dendritic cell line (DC 2.4). After cells were exposed to NIV (0.19-5 µmol) for 24 h, the production of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) was quantified. To further investigate the inflammatory cytokine production pathway, the possible involvement of mitogen-activated protein kinase (MAPK) pathways, such as ERK1/2, p-38, and JNK, in NIV exposure was analyzed using MAPK inhibitors and phosphorylation analyses...
April 14, 2024: Archives of Toxicology