Journals Toxicology and Applied Pharmac...

Toxicology and Applied Pharmacology
Xinwei Wan, Xudong Cui, Xiang Wang, Mingyang Feng, Shinan Wei, Jia Yu, Sha Cheng, Heng Luo, Jianxin Hu
"White pollution" has a significant impact on male reproduction. Di-n-butyl phthalate (DBP) is one of the most important factors in this type of pollution. Currently, research from international sources has demonstrated the significant reproductive toxicity of DBP. However, most of these studies have focused mainly on hormones expression at the protein and mRNA levels and the specific molecular targets of DBP and its mechanisms of action remain unclear. In this study, we established a Sprague Dawley pregnant mouse model exposed to DBP, and all male offspring were immediately euthanized at birth and bilateral testes were collected...
April 15, 2024: Toxicology and Applied Pharmacology
M Estefanía González-Alvarez, Imaobong Inyang, Aileen F Keating
Obesity impairs oocyte quality, fertility, pregnancy maintenance, and is associated with offspring birth defects. The model ovotoxicant, 7,12-dimethylbenz[a]anthracene (DMBA), causes ovarian DNA damage and follicle loss. Both DMBA-induced chemical biotransformation and the DNA damage response are partially attenuated in obese relative to lean female mice but whether weight loss could improve the DNA damage response to DMBA exposure has not been explored. Thus, at six weeks of age, C57BL/6 J female mice were divided in three groups: 1) Lean (L; n = 20) fed a chow diet for 12 weeks, 2) obese (O; n = 20) fed a high fat high sugar (HFHS) diet for 12 weeks and, 3) slim-down (S; n = 20)...
April 14, 2024: Toxicology and Applied Pharmacology
Daniela Gomes-Andrade, Bárbara Guerra-Carvalho, David F Carrageta, Raquel L Bernardino, Patrícia C Braga, Pedro F Oliveira, Maria de Lourdes Pereira, Marco G Alves
Atrazine (ATZ), a widely used herbicide with potent endocrine-disrupting properties, has been implicated in hormonal disturbances and fertility issues. Sertoli cells (SCs) play a crucial role in providing mechanical and nutritional support of spermatogenesis. Herein, we aimed to study the effects of environmentally relevant ATZ concentrations on the nutritional support of spermatogenesis provided by SCs. For that, mouse SCs (TM4) were exposed to increasing ATZ concentrations (in μg/L: 0.3, 3, 30, 300, or 3000)...
April 10, 2024: Toxicology and Applied Pharmacology
Yi Chen, Tianyang Jin, Mengpei Zhang, Bo Hong, Bo Jin, Chenghong Hu, Jiong Wang, Yue Chen, Lingxi Zhang, Yi Wang, Lijiang Huang
Inflammatory bowel disease (IBD) is characterized by recurrent inflammatory reactions in the intestinal mucosa, including ulcerative colitis (UC) and Crohn's disease (CD). The expression of Toll-like receptor 2 (TLR2) has been observed to increase during the progression of IBD. Flavokawain B (FKB), a natural chalcone with potent anti-inflammatory activity, exerts its effects through inhibition of the NF-κB pathway. In this study, we aimed to investigate the effects and mechanisms of FKB targeting TLR2 in IBD...
April 5, 2024: Toxicology and Applied Pharmacology
Ruxia Liu, Changfu Yang, Xin Yang, Jia Yu, Wenchao Tang
As a protein kinase inhibitor, cantharidin (CTD) exhibits antitumor activities. However, CTD is highly toxic, thereby limiting clinical applications. Moreover, relatively few studies have investigated CTD-induced reproductive toxicity, thus the underlying mechanism remains unclear. In this study, the toxic effects of CTD on mouse testis were confirmed in vivo and the potential mechanism was predicted by network toxicology (NT) and molecular docking technology. Proteins involved in the signaling pathways and core targets were verified...
April 4, 2024: Toxicology and Applied Pharmacology
Jiang He, Yajing Chen, Wen Zhong, Lu Jun, Dong Chen, Hui Cheng, Wang Mei
Asparaginase-associated pancreatitis (AAP) is a severe and potentially life-threatening drug-induced pancreas targeted toxicity in the combined chemotherapy of acute lymphoblastic leukemia among children and adolescents. The toxicological mechanism of AAP is not yet clear, and there are no effective preventive and treatment measures available clinically. Fibroblast growth factor 21 (FGF21) is a secretory hormone that regulates lipid, glucose, and energy metabolism balance. Acinar tissue is the main source of pancreatic FGF21 protein and plays an important role in maintaining pancreatic metabolic balance...
April 4, 2024: Toxicology and Applied Pharmacology
Christina Kamp-Jensen, Louise Norgil Donslund, Bjarne Styrishave, Rigmor Højland Jensen, Connar Stanley James Westgate
BACKGROUND: Idiopathic intracranial hypertension (IIH) is a disease characterized by elevated intracranial pressure (ICP) and is a disease of young females. The first line pharmacological treatments include acetazolamide and topiramate and given the nature of IIH patients and the dosing regimen of these drugs, their effect on the endocrine system is important to evaluate. We aimed to assess the effects of acetazolamide and topiramate on steroid profiles in relevant endocrine tissues. METHODS: Female Sprague Dawley rats received chronic clinically equivalent doses of acetazolamide or topiramate by oral gavage and were sacrificed in estrus...
April 3, 2024: Toxicology and Applied Pharmacology
Xiao-Yi Chen, Li Wang, Xiao Ma, Fan Yang, Xiao Wang, Peng Xu, Li-Li Xu, Bin Di
Fentanyl, a critical component of illicit drugs, poses a severe threat to public health, exacerbating the drug problem due to its potential fatality. Herein, we present two novel haptens designed with different attachment sites conjugated to keyhole limpet hemocyanin (KLH), aiming to develop an efficacious vaccine against fentanyl. KLH-Fent-1 demonstrated superior performance over KLH-Fent-2 in antibody titer, blood-brain distribution, and antinociceptive tests. Consequently, we immunized mice with KLH-Fent-1 to generate fentanyl-specific monoclonal antibodies (mAbs) using the hybridoma technique to compensate for the defects of active immunization in the treatment of opioid overdose and addiction...
April 1, 2024: Toxicology and Applied Pharmacology
S P Ramya Ranjan Nayak, Seenivasan Boopathi, Muniswamy Chandrasekar, B Yamini, Vellapandian Chitra, Bader O Almutairi, Selvaraj Arokiyaraj, Ajay Guru, Jesu Arockiaraj
Indole-3-acetic acid (IAA) is the most widely utilized plant growth regulator. Despite its extensive usage, IAA is often overlooked as an environmental pollutant. Due to its protein-binding nature, it also functions as a uremic toxin, contributing to its association with chronic kidney disease (CKD). While in vitro and epidemiological research have demonstrated this association, the precise impact of IAA on cardiovascular disease in animal models is unknown. The main objective of this study is to conduct a mechanistic analysis of the cardiotoxic effects caused by IAA using male Wistar albino rats as the experimental model...
March 28, 2024: Toxicology and Applied Pharmacology
Zhenchuan Ma, Yuefeng Ma, Jie Feng, Zhengshui Xu, Chuantao Cheng, Jie Qin, Shaomin Li, Jiantao Jiang, Ranran Kong
N-myc downstream-regulated gene 2 (NDRG2) has been recognised as a negative regulator of the progression of numerous tumours, yet its specific role in small-cell lung carcinoma (SCLC) is not fully understood. The purpose of the current study was to investigate the biological role and mechanism of NDRG2 in SCLC. Initial investigation using the Gene Expression Omnibus (GEO) dataset revealed marked downregulation of NDRG2 transcripts in SCLC. The decreased abundance of NDRG2 in SCLC was verified by examining clinical specimens...
March 25, 2024: Toxicology and Applied Pharmacology
Wei Wang, Shufan Chang, Xi He, XiaoLei Zhou, Pingping Shang, Yusong Chen, Xiaoke Wang, Lijuan Chen, Qiao Zhang, Yahong Qiao, Feifei Feng
This study aims to explore the impact and underlying mechanism of sulforaphane (SFN) intervention on the migration and invasion of lung adenocarcinoma induced by 7, 8-dihydroxy-9, 10-epoxy-benzo (a) pyrene (BPDE). Human lung adenocarcinoma A549 cells were exposed to varying concentrations of BPDE (0.25, 0.50, and 1.00 μM) and subsequently treated with 5 μM SFN. Cell viability was determined using CCK8 assay, while migration and invasion were assessed using Transwell assays. Lentivirus transfection was employed to establish NLRP12 overexpressing A549 cells...
March 25, 2024: Toxicology and Applied Pharmacology
Li Tao, Yu-Feng Xue, Fei-Fei Sun, Xue He, Hong-Qian Wang, Cheng-Cheng Tong, Cheng Zhang, De-Xiang Xu, Xi Chen
Ferroptosis has been shown to be involved in carbon tetrachloride (CCl4 )-induced acute liver injury (ALI). The mitochondrion-targeted antioxidant MitoQ can eliminate the production of mitochondrial reactive oxygen species (mtROS). This study investigated the role of MitoQ in CCl4 -induced hepatocytic ferroptosis and ALI. MDA and 4HNE were elevated in CCl4 -induced mice. In vitro, CCl4 exposure elevated the levels of oxidized lipids in HepG2 cells. Alterations in the mitochondrial ultrastructure of hepatocytes were observed in the livers of CCl4 -evoked mice...
March 24, 2024: Toxicology and Applied Pharmacology
Nancy H C Loos, Rolf W Sparidans, Paniz Heydari, Viët Bui, Maria C Lebre, Jos H Beijnen, Alfred H Schinkel
The highly selective Spleen Tyrosine Kinase (SYK) inhibitors entospletinib and lanraplenib disrupt kinase activity and inhibit immune cell functions. They are developed for treatment of B-cell malignancies and autoimmunity diseases. The impact of P-gp/ABCB1 and BCRP/ABCG2 efflux transporters, OATP1a/1b uptake transporters and CYP3A drug-metabolizing enzymes on the oral pharmacokinetics of these drugs was assessed using mouse models. Entospletinib and lanraplenib were orally administered simultaneously at moderate dosages (10 mg/kg each) to female mice to assess the possibility of examining two structurally and mechanistically similar drugs at the same time, while reducing the number of experimental animals and sample-processing workload...
March 23, 2024: Toxicology and Applied Pharmacology
Gloria Melzi, Lorenzo Massimi, Maria Agostina Frezzini, Martina Iulini, Naima Tarallo, Matteo Rinaldi, Marco Paglione, Emma Nozza, Federica Crova, Sara Valentini, Gianluigi Valli, Francesca Costabile, Silvia Canepari, Stefano Decesari, Roberta Vecchi, Marina Marinovich, Emanuela Corsini
Particulate Matter (PM) is a complex and heterogeneous mixture of atmospheric particles recognized as a threat to human health. Oxidative Potential (OP) measurement is a promising and integrative method for estimating PM-induced health impacts since it is recognized as more closely associated with adverse health effects than ordinarily used PM mass concentrations. OP measurements could be introduced in the air quality monitoring, along with the parameters currently evaluated. PM deposition in the lungs induces oxidative stress, inflammation, and DNA damage...
March 22, 2024: Toxicology and Applied Pharmacology
Tiancang Han, Zhaoyang Sun, Hongbo Zhang, Yuhan Zhao, Anhui Jiao, Qingshan Gao
3-nitropropionic acid (3-NPA), a toxic metabolite produced by mold, is mainly found in moldy sugarcane. 3-NPA inhibits the activity of succinate dehydrogenase that can induce oxidative stress injury in cells, reduce ATP production and induce oxidative stress in mouse ovaries to cause reproductive disorders. Ursolic acid (UA) has a variety of biological activities and is a pentacyclic triterpene compound found in many plants. This experiment aimed to investigate the cytotoxicity of 3-NPA during mouse oocyte in vitro maturation and the protective effects of UA on oocytes challenged with 3-NPA...
March 21, 2024: Toxicology and Applied Pharmacology
Mohd Rihan, Shyam Sunder Sharma
Myocardial infarction (MI) or heart attack arises from acute or chronic prolonged ischemic conditions in the myocardium. Although several risk factors are associated with MI pathophysiology, one of the risk factors is an imbalance in the oxygen supply. The current available MI therapies are still inadequate due to the complexity of MI pathophysiology. Pyruvate kinase M2 (PKM2) has been implicated in numerous CVDs pathologies. However, the effect of specific pharmacological intervention targeting PKM2 has not been studied in MI...
March 21, 2024: Toxicology and Applied Pharmacology
Juanjuan Zou, Shengnan Qi, Xiaojing Sun, Yijing Zhang, Yan Wang, Yanzhong Li, Ze-Hua Zhao, Dapeng Lei
BACKGROUND: Obstructive sleep apnea (OSA) is considered to be an important contributor of dyslipidemia. However, there lacks observational studies focusing on the potential effect of lipid management on OSA risk. Thus, we aimed to investigate the genetic association of lipid-modifying therapy with risk of OSA. METHODS: A drug-target mendelian randomization (MR) study using both cis-variants and cis-expression quantitative trait loci (eQTLs) of lipid-modifying drug targets was performed...
March 21, 2024: Toxicology and Applied Pharmacology
Swagata Pal, Shaheen Wasil Kabeer, Kulbhushan Tikoo
Adenocarcinoma, the predominant subtype of non-small cell lung cancer (NSCLC), poses a significant clinical challenge due to its prevalence and aggressive nature. Gefitinib, an epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor is often susceptible to development of resistance despite being the preferred treatment option for NSCLC. In this study, we investigated the potential of L-Methionine in enhancing the cytotoxicity of Gefitinib and preventing resistance development. In vitro experiment employing the H1975 cell line demonstrated a notable enhancement in cytotoxic efficacy when L-Methionine (10 mM) was combined with Gefitinib, as indicated by a substantial reduction in IC50 values (155...
March 21, 2024: Toxicology and Applied Pharmacology
Mikito Hirakata, Emi Tomikawa, Chizuka Sakai, Masashi Uchida, Tsubasa Okano, Reiko Shimozono, Masakatsu Kawai, Shoichi Itaba, Lisa Munakata, Ryo Suzuki, Keiyu Oshida
Anthracycline anti-cancer drugs have been widely used in the treatment of several cancers; however, their use is limited by adverse effects (AEs). Alopecia is a common AE that is minimally invasive, but adversely affects mental health and reduces quality of life (QoL). Hand-foot syndrome (HFS) is a dose-limiting AE of DOXIL, a liposomal formulation of doxorubicin (DOX). Although it is not a life-threatening condition, HFS affects function and reduces QoL. TXB-001 is a new candidate polymer-conjugated anthracycline anti-cancer drug, and modified and optimized polymerized pirarubicin (THP), known as P-THP, is expected to have low toxicity and high efficacy...
March 21, 2024: Toxicology and Applied Pharmacology
Rama Malaviya, Jaclynn Meshanni, Vasanthi R Sunil, Alessandro Venosa, Changjiang Guo, Elena V Abramova, Kinal N Vayas, Chenghui Jiang, Jessica A Cervelli, Andrew J Gow, Jeffrey D Laskin, Debra L Laskin
Nitrogen mustard (NM) is a toxic vesicant that causes acute injury to the respiratory tract. This is accompanied by an accumulation of activated macrophages in the lung and oxidative stress which have been implicated in tissue injury. In these studies, we analyzed the effects of N-acetylcysteine (NAC), an inhibitor of oxidative stress and inflammation on NM-induced lung injury, macrophage activation and bioenergetics. Treatment of rats with NAC (150 mg/kg, i.p., daily) beginning 30 min after administration of NM (0...
March 19, 2024: Toxicology and Applied Pharmacology
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read

Save your favorite articles in one place with a free QxMD account.


Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.