Milagrosa López-Riego, Magdalena Płódowska, Milena Lis-Zajęcka, Kamila Jeziorska, Sylwia Tetela, Aneta Węgierek-Ciuk, Daniel Sobota, Janusz Braziewicz, Lovisa Lundholm, Halina Lisowska, Andrzej Wojcik
Candidate ionising radiation exposure biomarkers must be validated in humans exposed in vivo. Blood from patients undergoing positron emission tomography-computed tomography scan (PET-CT) and skeletal scintigraphy (scintigraphy) was drawn before (0 h) and after (2 h) the procedure for correlation analyses of the response of selected biomarkers with radiation dose and other available patient information. FDXR, CDKN1A, BBC3, GADD45A, XPC, and MDM2 expression was determined by qRT-PCR, DNA damage (γH2AX) by flow cytometry, and reactive oxygen species (ROS) levels by flow cytometry using the 2', 7'-dichlorofluorescein diacetate test in peripheral blood mononuclear cells (PBMC)...
June 19, 2023: Radiation and Environmental Biophysics