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Scandinavian Journal of Clinical and Laboratory Investigation

Hajnal Zsuzsanna Balla, Elvar Theodorsson, Jakob O Ström
Extensive research has been devoted to developing methods for assessing core body temperature, and to determine which method is most accurate. A number of wireless dermal thermometers for home use are presently available, but their relation to core body temperature and suitability for use in clinical research has hitherto not been assessed. The current study aimed to evaluate such thermometers by comparing them to the results of a rectal thermometer. Four wireless dermal thermometers for home use (FeverSmart, iThermonitor, Quest Temp Sitter, and Thermochron iButton) were applied to 15 patients during 24 h, and rectal temperature was measured at four occasions...
March 18, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Dan Tudor Eniu, Florina Romanciuc, Corina Moraru, Iulian Goidescu, Daniela Eniu, Adelina Staicu, Claudiu Rachieriu, Rareş Buiga, Carmen Socaciu
This study was targeted on a metabolomic approach to compare the blood serum free amino acid profiles and concentration of confirmed breast cancer (stages I-III) patients to healthy controls in order to establish reliable biomarkers of early detection and prediction of breast cancer. The ultra-high-performance liquid chromatography coupled with mass spectrometry using positive ionization electrospray was applied for the picoline-derivatized serum free amino acids using the EZ:faastTM kit. Multivariate statistical analysis principal component analysis, partial least squares discrimination analysis and univariate analysis were applied in order to discriminate between patient groups and putative amino acid biomarkers for breast cancer...
March 16, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Stéphanie Roullet, Laurent Weinmann, Sylvie Labrouche, Chloé Gisbert-Mora, Matthieu Biais, Philippe Revel, Geneviève Freyburger
Hyperfibrinolysis contributes to the pathophysiology of trauma-induced coagulopathy. At present, systematic administration of tranexamic acid (TXA) is recommended in all patients in the early phase of trauma. However, there is some debate regarding whether TXA is beneficial in all trauma patients. A rapid and accurate tool to diagnose hyperfibrinolysis may be useful for tailoring TXA treatment. We conducted a proof-of-concept study of consecutive adult trauma patients. A first blood sample was obtained at the time of pre-hospital care (T1)...
March 12, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Gowthami Gunapalasingham, Christine Frithioff-Bøjsøe, Morten Asp Vonsild Lund, Paula Louise Hedley, Cilius Esmann Fonvig, Maria Dahl, Oluf Pedersen, Michael Christiansen, Torben Hansen, Ulrik Lausten-Thomsen, Jens-Christian Holm
Thyroid-stimulating hormone (TSH) and thyroid hormones influence the functions of many organ systems, as well as child development and growth. Several studies have reported an association between ethnicity and thyroid hormones. This study aims to explore pediatric serum concentrations of TSH, free triiodothyronine (fT3), and free thyroxine (fT4) and their relation to age and sex and subsequently to present pediatric reference intervals from healthy Danish/North-European white children. A population-based cohort in Denmark of 2411 (1435 girls) healthy school children and adolescents aged 6...
March 12, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Anna Machoy-Mokrzyńska, Zuzanna Starzyńska-Sadura, Violetta Dziedziejko, Krzysztof Safranow, Mateusz Kurzawski, Katarzyna Leźnicka, Violetta Sulżyc-Bielicka, Alina Jurewicz, Andrzej Bohatyrewicz, Monika Białecka
Pain is one of the most interdisciplinary clinical symptoms of a disease. The aim of the study was to evaluate the association of COMT gene polymorphism with pain perception in patients after total hip replacement (THR). The study included 195 patients qualified for surgical treatment (THR) due to osteoarthritis. Patients previously undergoing hip replacement subsequently underwent multimodal pain management therapy, in accordance with the recommendations for treating postoperative pain. The intensity of pain was measured three times at pre-defined time intervals: 1...
March 1, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Martin Racek, Jaroslav Racek, Ivana Hupáková
Urolithiasis is a frequent and in many cases serious disease. Proper analysis of kidney stone composition is crucial for appropriate treatment and prevention of disease recurrence. In this work, scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy was applied for a study of 30 samples covering the most common types of human kidney stones. The results are analyzed and evaluated in terms of applicability of the method for both routine kidney stone analysis as well as collecting of specific data...
March 1, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Sandra M Ramírez-Meza, Montserrat Maldonado-González, Zamira H Hernández-Nazara, Erika Martínez-López, Saúl Ocampo-González, Lucina Bobadilla-Morales, José R Torres-Baranda, Bertha Ruíz-Madrigal
Most quantitative real-time PCR (qPCR) detection methods use two types of chemistries to measure the expression levels of ChREBP isoforms, hydrolysis probes for ChREBPα and SYBR Green for ChREBPβ. Hydrolysis probes are not available to determine the ChREBPβ isoform. The aim of this study was to develop a qPCR assay based only on hydrolysis probes for both ChREBP isoforms. Liver and adipose tissue biopsies from patients undergoing elective cholecystectomy surgery were used to perform qPCR. To validate this assay, the results were compared with sequencing and High Resolution Melting (HRM) PCR assays...
February 27, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Araceli Jarquin Campos, Lorenz Risch, Marc Baumann, Mette-Triin Purde, Sebastian Neuber, Harald Renz, Beatrice Mosimann, Luigi Raio, Markus Mohaupt, Daniel Surbek, Martin Risch
Shrunken pore syndrome (SPS) is a condition that manifests itself as the decreased renal clearance of low-molecular-weight proteins but normal clearance of creatinine. Pregnant women with evidence of SPS during the first trimester have an increased risk of developing preeclampsia (PE). The nitric oxide (NO) metabolism markers arginine and ADMA, especially their ratio (Arg/ADMA), are recognized markers of endothelial dysfunction. The aim of this nested case-control study was to establish first-trimester reference intervals (RI) for markers of NO metabolism and to study these markers in women with evidence of SPS at the end of the first trimester...
February 20, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Tania Deis, Louise Balling, Søren Boesgaard, Kasper Rossing, Morten Schou, Peter Oturai, Emil Wolsk, Finn Gustafsson
The interaction between hemodynamics and kidney function in heart failure (HF) is incompletely understood. We investigated the association between invasive hemodynamic parameters and measured glomerular filtration rate (mGFR) by plasma clearance of 51-chromium-labeled ethylenediamine tetra-acetic acid (51 Cr-EDTA) in patients with advanced HF and tested the hypothesis that patients with reduced mGFR have lower cardiac index (CI) and mean arterial pressure (MAP) as well as higher central venous pressure (CVP) and pulmonary capillary wedge pressure (PCWP)...
February 20, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Tuomas Kerola, Jenni Kauppi, Laura Sares-Jäske, Olli Anttonen, M Juhani Junttila, Heikki V Huikuri, Tuomo Nieminen, Harri Rissanen, Markku Heliövaara, Markku J Kauppi, Paul Knekt, Antti Eranti
The debate whether an elevated level of serum uric acid (SUA) is an independent marker of cardiovascular risk is still going on. We examined morbidity and mortality related to SUA and hyperuricemia in a well-characterized population with very long follow-up. Study included 4696 participants (aged 30-59 years at baseline) of the coronary heart disease (CHD) Study of the Finnish Mobile Clinic Health Examination Survey. Adjusted hazard ratios (HRs) of hyperuricemia (defined as ≥360 µmol/l and ≥420 µmol/l) and SUA quintiles for mortality and adverse cardiovascular outcomes are reported...
February 19, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Arne Åsberg, Hilde Hegseth, Maria Averina, Lena Løfblad
Using CoaguChek to measure PT-INR and comparing the results with those from the hospital laboratory, some patients get consistent results while others do not. The extent of this problem is unknown. Our study aimed to quantify the between-subject variation of the systematic PT-INR difference between CoaguChek and a hospital laboratory method. We used register data with PT-INR results from both CoaguChek and a hospital laboratory method (STA-SPA+) in samples taken simultaneously from 108 patients. After excluding five patients with outlying results, we used mixed-effects models to estimate individual slopes and intercepts to describe the systematic relationship between the two methods for each patient, and calculated the fraction of patients having a systematic difference greater than 0...
February 19, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Søren Kristiansen, Lennart Friis-Hansen
Standardization programs for thyroid hormones have revealed bias between immunochemical methods and the reference method ED-ID-LC/MS. Lack of standardization between methods, suboptimal reference intervals and replacement of serum with plasma may compromise the capability of the immunochemical thyroid methods to diagnose thyroid disease. To accommodate the demand for faster turn-around times for laboratory replies, we replaced serum with plasma on some serum CE marked thyroid methods. This forced us to do on-board analytical correction for the plasma total T4 (TT4) method on ADVIA Centaur® XP...
February 19, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Fredrik Uhlin, Anders Fernström, Marjo H J Knapen, Cees Vermeer, Per Magnusson
Rapid progression of vascular calcification (VC) in hemodialysis (HD) patients is caused by several factors including inflammation and an imbalance between active inducers and inhibitors of VC. Growing evidence shows that online hemodiafiltration (ol-HDF), a combination of diffusive and convective solute transport, has positive effects on the uremic environment that affects patients on dialysis. However, we recently reported that serum 25-hydroxyvitamin D (25(OH)D) decreased after a switch from HD to ol-HDF...
February 18, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Antonija Perović, Maja Dolčić
The aim of this study was to examine the influence of hemolysis on 25 clinical chemistry parameters and to compare the resulting bias with clinically significant differences and the manufacturer's specifications. Using freeze-thawing of the treated blood aliquot of each subject (N = 17), four hemolysis levels were prepared with hemolysis index (HI) and hemoglobin concentration as follows: (+)=0.5-0.99 g/L, (2+)=1-1.99 g/L, (3+)=2-2.99 g/L and (4+)=3-4.99 g/L. All analytes were tested on the Beckman Coulter AU480 analyzer using proprietary reagents...
February 15, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Paul Pettersson-Pablo, Torbjörn K Nilsson, Lars H Breimer, Anita Hurtig-Wennlöf
The primary aim was to appraise the relationship between body fat percentage and the inflammatory markers C-reactive protein (CRP) and orosomucoid in a population of young, non-smoking, healthy, Swedish adults, without any chronic diseases. A secondary aim was to compare whether these associations differed between the women using estrogen contraceptives and those who did not. We assessed the association in linear regression models between body fat percentage based on a bio-impedance measurement and plasma concentrations of CRP and orosomucoid in men and women aged 18-26 years, n = 834...
February 15, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Erik Herou, Alain Dardashti, Shahab Nozohoor, Igor Zindovic, Per Ederoth, Anders Grubb, Henrik Bjursten
Shrunken pore syndrome (SPS) is a condition in which estimated glomerular filtration rate (eGFR) based upon cystatin C is lower than eGFR based upon creatinine. It has been associated with increased mortality even in the presence of normal GFR in both a cardiac surgical population and a general population. No systematic studies of the variation in eGFRcystatin C /eGFRcreatinine -ratio used for SPS diagnosis have been published. This study aims to evaluate whether early and midterm mortality following elective cardiac surgery varies with the ratio used to identify SPS...
February 15, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Aleksandra R Vukašinović, Jelena M Kotur-Stevuljević, Vid Mlakar, Miron D Sopić, Zorica P Cvetković, Miloš R Petković, Vesna V Spasojević-Kalimanovska, Nataša B Bogavac-Stanojević, Barbara Ostanek
Telomerase is RNA directed polymerase which acts as reverse transcriptase based on its own RNA component. It is considered to be involved in the pathology of many diseases and is recognized as a potential biomarker. The aims were to determine the sample storage conditions and the time frame for samples analysis, then to prove reliability of enzyme activity measurement with real-time telomeric repeat amplification protocol (TRAP) and to evaluate the suitable standard samples for telomerase activity measurements...
February 15, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Magnus T Jensen
Assessment of heart rate has been used for millennia as a marker of health. Several studies have indicated that low resting heart rate (RHR) is associated with health and longevity, and conversely, a high resting heart to be associated with disease and adverse events. Longitudinal studies have shown a clear association between increase in heart rate over time and adverse events. RHR is a fundamental clinical characteristic and several trials have assessed the effectiveness of heart rate lowering medication, for instance beta-blockers and selective sinus node inhibition...
February 14, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Arne Åsberg, Lena Løfblad, Amela Felic, Gunhild Garmo Hov
Calprotectin in plasma and blood might prove to be a useful biomarker of inflammation and infection; however, automated methods for analysing the concentration of calprotectin in those materials are lacking. We have validated a fully automated turbidimetric method and present health-related reference limits. Calprotectin was measured by Siemens Advia XPT with the Bühlmann fCAL® turbo test (Bühlmann Laboratories AG, Schönenbuch, Switzerland), a particle enhanced turbidimetric immunoassay for quantification of calprotectin in fecal extracts...
February 14, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Chaicharoen Tantanate, Ladawan Khowawisetsut, Kasama Sukapirom, Kovit Pattanapanyasat
The aim of this study was to evaluate the performance of automated impedance platelet counts by Beckman Coulter LH780 (PLT-LH), Sysmex XN-3000 (PLT-XNi) and fluorescence method by Sysmex XN-3000 (PLT-F) in patients with acute leukemia. Blood specimens were subjected to platelet measurements by evaluated methods and then compared against the international reference method (IRM). Eighty-two blood specimens were included. Bland-Altman plots of the differences between the evaluated methods and IRM demonstrated mean biases of PLT-LH, PLT-XNi and PLT-F of 9 × 109 /L, 11 × 109 /L and 2 × 109 /L, respectively...
February 14, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
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