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Molecular Biology Reports

Bin Xiao, Jianfeng Hang, Ting Lei, Yongyin He, Zhenzhan Kuang, Li Wang, Lidan Chen, Jia He, Weiyun Zhang, Yang Liao, Zhaohui Sun, Linhai Li
Few prognostic indicators with differential expression have been reported among the differing ER statuses. We aimed to screen important breast cancer prognostic genes related to ER status and to construct an efficient prognostic prediction system. mRNA expression profiles were downloaded from TCGA and GSE70947 dataset. Two hundred seventy-one overlapping differentially expressed genes (DEGs) between the ER- and ER+ breast cancer samples were identified. Among the 271 DEGs, 109 prognostically relevant mRNAs were screened...
March 19, 2019: Molecular Biology Reports
Beema Shafreen Rajamohamed, Seema Siddharthan
In the present study, Amukkara Chooram (AC) a well known herbal medicine was investigated for their antibiofilm efficacy against biofilm of Candida albicans. The biofilm inhibitory concentration of 20 µg/mL of AC showed promising effect by inhibiting the biofilm upto 60%. Morphogenic transition state of C. albicans from yeast cells to hyphal transition was prevented by AC was revealed from light microscopic images. In addition, the inhibition of yeast hyphae was examined in the induction medium supplemented with AC...
March 18, 2019: Molecular Biology Reports
Arash Moradi, Nasrin Ziamajidi, Abolfazl Ghafourikhosroshahi, Roghayeh Abbasalipourkabir
The increasing application of titanium dioxide nanoparticles (NTiO2 ) in life and the toxicity potential of these nanoparticles have raised concerns about their detrimental effects on human health. This study was conducted to investigate the hepatoprotective effects of vitamin E and vitamin A against hepatotoxicity induced by NTiO2 in rats. Thirty-six male Wistar rats were randomly divided into six groups of six rats each. Intoxicated group received 300 mg/kg NTiO2 for two weeks by gavage. Groups treated with vitamin E (100 IU/kg), vitamin A (100 IU/kg) and mixture of these vitamins were orally administered for 3 weeks (started 7 days before NTiO2 administration)...
March 18, 2019: Molecular Biology Reports
Ahmed Fathy Youssef, Nabil Ahmed Younes, Muhammad Youssef
Assessment of genetic diversity has an efficient role in plant breeding and improvement programs. There is a limit number of investigations dealing with the evaluation of genetic diversity in Jew's mallow (Corchorus olitorius L.), despite its valuable importance as a leafy vegetable and a delicious dish rich in vitamins and antioxidants. Therefore, in this study, 18 landraces of Jew's mallow-collected from different locations in Egypt-were used for genetic diversity assessment based on morphophysiological and molecular evaluations...
March 18, 2019: Molecular Biology Reports
Bowen Wang, Bingfen Huang, Junmei Chen, Wenpeng Li, Ling Yang, Lunguang Yao, Qiuhong Niu
Colonization resistance is an important attribute for bacterial interactions with hosts, but the mechanism is still not completely clear. In this study, we found that Phytobacter sp. SCO41T can effectively inhibit the in vivo colonization of Bacillus nematocida B16 in Caenorhabditis elegans, and we revealed the colonization resistance mechanism. Three strains of colonization-resistant bacteria, SCO41T , BX15, and BC7, were isolated from the intestines of the free-living nematode C. elegans derived from rotten fruit and soil...
March 16, 2019: Molecular Biology Reports
P Guru Vishnu, T K Bhattacharya, Bharat Bhushan, Pushpendra Kumar, R N Chatterjee, Chandan Paswan, K Dushyanth, D Divya, A Rajendra Prasad
Gene silencing by RNA interference is extensively used reverse genetic approach to analyse the implications of any gene in mammalian systems. The silencing of the Activin type IIB receptor belonging to transforming growth factor beta superfamily has demonstrated increase in muscle growth in many species. We designed five short hairpin RNA constructs targeting coding region of chicken ACTRIIB. All the shRNAs were transfected into chicken embryo fibroblast cells and evaluated their silencing efficiency by real time PCR and western blotting...
March 16, 2019: Molecular Biology Reports
Mary García-Acero, Mónica Molina, Olga Moreno, Andrea Ramirez, Catalina Forero, Camila Céspedes, Juan Carlos Prieto, Jaime Pérez, Fernando Suárez-Obando, Adriana Rojas
Two sisters phenotypically normal females, presenting with tumor abdominal mass with histopathological findings of teratoma and gonadoblastoma associated to 46,XY male-to-female sex reversal syndrome, secondary to a duplication in DAX-1, possibly inherited of maternal gonadal mosaicism. Copy number variation and functional effects of the duplication were done by MLPA multiplex ligation-dependent probe amplification and real time PCR. DAX-1, also known as dosage sensitive sex reversal gene (DSS), is considered the most likely candidate gene involved in XY gonadal dysgenesis when overexpressed...
March 16, 2019: Molecular Biology Reports
Aleksandra Sentkowska, Krystyna Pyrzyńska
The antioxidant interactions between selenium species and tea polyphenols were investigated using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals, cupric reducing antioxidant capacity (CUPRAC) and Folin-Ciocalteu (FC) assay. Se(IV) exhibited the lowest antioxidant properties in comparison to other selenium compounds in all assays. The highest reducing power was obtained for SeMet, while the highest ability to scavenging DPPH radicals for MeSeCys. The results obtained experimentally for the mixtures containing selenium species and green or black tea infusion were compared with theoretical values calculated by adding up the effects of both individual components analyzed separately...
March 13, 2019: Molecular Biology Reports
Mousumi Saikia, Dipali Devi
The Indian Eri silkworm, Philosamia ricini Hutt, a commercial silk producing insect, is believed to have originated in the Brahmaputra valley of Assam. In this study, the genetic diversity and phylogenetic relationships of six morphs of Eri silkworm viz. white plain, white zebra, white spotted, blue plain, blue zebra and blue spotted collected from different geographical locations of North-East India were investigated by using random amplified polymorphic DNA (RAPD) and the first internal transcribed spacer region (ITS1)...
March 13, 2019: Molecular Biology Reports
Cheng Libao, Liu Huiying, Han Yuyan, Li Shuyan
MicroRNA (miRNA)-regulated gene expression plays an important role in various plant metabolic processes. Although adventitious roots are critical to plant growth in lotus, the role of miRNA in AR formation remains unclear. Expression profiling of miRNAs was carried out during three different developmental stages of ARs in lotus: no induction of AR stage, initial stage of ARs, and maximum number of ARs. These data are referenced with the whole lotus genome as already identified through high-throughput tag-sequencing...
March 12, 2019: Molecular Biology Reports
Abdulahad Dogan, Ozgur Ozan Anuk
Chinar (Platanus orientalis L.) is used in folk medicine against tooth and knee pain, wounds, inflammation, and stomach discomfort; however, the effects of P. orientalis leaf (PO-leaf) infusion on the liver and kidney are unknown. The aim of this study was to investigate the phytochemical composition and antioxidant properties of an infusion obtained from dried P. orientalis leaves against ethanol-induced oxidative stress (OS) in rats. After a toxicity test, thirty male Wistar rats were divided into five groups: Control, Ethanol 20%, Ethanol 20% + Silymarin (10 mg/kg), Ethanol 20% + PO-20 mg/mL infusion, and Ethanol 20% + PO-60 mg/mL infusion...
March 12, 2019: Molecular Biology Reports
Azram Tahoor, Jamal Ahmad Khan, Sahil Mahfooz
Information on the level and distribution of genetic variation is important for conservation plan of captive population of an endangered species such as tiger and cheetah. We assayed the frequency of microsatellites in the genomic and genic sequences of wild cats (Panthera tigris, Acinonyx jubatus) and compared it with the domestic cat (Felis catus). Frequency, relative abundance and density of microsatellites were highest in the domestic cat when compared with wild cats. The frequency of microsatellites was positively correlated with the G+C content of genomic and genic sequences...
March 12, 2019: Molecular Biology Reports
Nicolas Oury, Marc Léopold, Hélène Magalon
Holothuria (Microthele) fuscogilva (Cherbonnier, 1980), Holothuria sp. type "Pentard" and Holothuria (Microthele) nobilis (Selenka, 1867) are three tropical sea cucumber taxa that are heavily fished worldwide for the beche-de-mer trade market. In order to investigate the population genetic structure, diversity and connectivity of these taxa, 16, 19 and 25 microsatellite loci were isolated from H. fuscogilva, Holothuria sp. type "Pentard" and H. nobilis DNA libraries, respectively. These loci were tested on 94, 60 and eight individuals of the respective species, collected from the Seychelles...
March 12, 2019: Molecular Biology Reports
Milad Mohammadzadeh, Saeid Ghorbian, Mohammad Nouri
Repeated implantation failure (RIF) is one the most common causes which showed during IVF (In vitro fertilization) procedure. We aim to evaluate the possibility role of nucleotide changes in rs1042522 (R72P; G/C) and rs17878362 (Ins16bp; N/D) of P53 gene in patients with RIF. In a case-control survey, we have considered 200 women, consisting of 100 cases with RIF and 100 women with the normal pregnancy. In order to determine the genotype frequencies, we used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method...
March 11, 2019: Molecular Biology Reports
Elumalai Sivamani, Samson Nalapalli, Anna Prairie, David Bradley, Lee Richbourg, Tim Strebe, Tara Liebler, Daolong Wang, Qiudeng Que
Phosphinothricin acetyltransferase gene (pat) is an important selectable marker and also a key herbicide trait gene in several commercial products. In maize, the transformation frequency (TF) using pat as a selectable marker is the lowest among the commonly used marker options including epsps, pmi or ppo. Low pat transformation efficiency can become a major bottleneck in our ability to efficiently produce large numbers of events, especially for large molecular stack vectors with multiple trait gene cassettes...
March 11, 2019: Molecular Biology Reports
Petr Šálek, Marcela Filipová, Daniel Horák, Vladimír Proks, Olga Janoušková
The efficiency of solid phase extraction (SPE) of DNA on polymer particles is limited by the features of the applied solid support, such as size, hydrophilicity, and functionality and their application in SPE also requires additional steps and compounds to finally obtain sufficient amount of high-quality DNA. The present study describes a preparation of sub-micrometer monodisperse poly(methacrylic acid-co-ethylene dimethacrylate) (PME) particles by precipitation polymerization. The effect of the ethylene dimethacrylate (EDMA) crosslinker concentration on morphology and particle size, which varied from 730 to 900 nm, was investigated...
March 11, 2019: Molecular Biology Reports
Patrycja Bronowicka-Adamska, H Jurkowska, A Gawda, P Skalska, K Nazimek, J Marcinkiewicz, Maria Wróbel
Murine macrophages of the J774A.1 line are hydrogen sulphide-producing cells with the primary role of γ-cystathionase (CTH) and secondary role of 3-mercaptopyruvate sulfurtransferase (limited by cysteine availability) and with a negligible role of cystathionine β-synthase (CBS) in H2 S generation. J774A.1 cells stimulation with lipopolysaccharide (LPS) or interferon-gamma (IFNγ) resulted in decreased H2 S levels after 24 h of incubation; however, they were restored to the control level after 48 h. Negligible CBS expression and activity in J774A...
March 11, 2019: Molecular Biology Reports
Xin Liu, Min Chen, Xue Zhou, Zhimin Cao
Two novel miRNAs were selected from a pre-constructed RNA library of Populus szechuanica infected with the foliar rust fungus Melampsora larici-populina in order to detect the genes regulated as targets of the miRNAs novel_mir_11 and novel_mir_357. The novel miRNAs were identified from P. szechuanica using stem-loop methods and their precursors were able to fold into a complete stem loop structure. The predicted target genes of the novel miRNAs were verified with RNA ligase-mediated 5' rapid amplification of cDNA ends (RLM-5'RACE)...
March 11, 2019: Molecular Biology Reports
F L Muniz, A M Ximenes, P S Bittencourt, S M Hernández-Rangel, Z Campos, T Hrbek, I P Farias
We isolated and characterized 10 new microsatellites loci for Paleosuchus trigonatus using ION TORRENT Sequencing Technology. We tested the transferability of these loci to three related species of the subfamily Caimaninae, and used these bi-parental markers to test population structure and genetic diversity of two populations of P. trigonatus impacted by hydroelectric dam construction on the Madeira (N = 16) and Xingu (N = 16) rivers. We also investigated the transferability of these markers to three related species: Paleosuchus palpebrosus (N = 5), Caiman crocodilus (N = 6) and Melanosuchus niger (N = 6)...
March 9, 2019: Molecular Biology Reports
Dmitry Alekseevich Sychev, Sherzod Pardaboevich Abdullaev, Karin Badavievich Mirzaev, Kristina Anatolevna Ryzhikova, Grigoriy Nikolaevich Shuyev, Zhannet Alimovna Sozaeva, Elena Anatolevna Grishina, Suleiman Nurattinovich Mammaev, Daniyal Musaevich Gafurov, Elena Yurievna Kitaeva, Vladimir Viktorovich Shprakh, Salavat Sheikhovich Suleymanov, Laura Zelimkhanovna Bolieva, Maryam Sultan-Hamitovna Sozaeva, Svetlana Mikhailovna Zhuchkova, Natalia Evgenievna Gimaldinova, Elena Eduardovna Sidukova, Anastasiia Valerievna Asoskova, Robert Borisovich Mumladze
This study was aimed to investigate the prevalence of the CES1 gene (c.1168-33A > C, rs2244613) polymorphism among 12 different ethnic groups living in Russia to provide a basis for future clinical studies concerning genetic determinants of dabigatran safety. The study involved 1630 apparently healthy, unrelated, and chronic medication-free volunteers of both genders from 12 different ethnic groups in Russia: 136 Russians, 90 Avars, 50 Dargins, 46 Laks, 120 Kabardians, 112 Balkars, 244 Ossetians, 206 Mari, 204 Mordvinians, 238 Chuvashes, 114 Buryats and 70 Nanays...
March 8, 2019: Molecular Biology Reports
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