Monica Gatti, Roberto Wurth, Guendalina Vito, Alessandra Pattarozzi, Chiara Campanella, Stefano Thellung, Lorella Maniscalco, Raffaella De Maria, Valentina Villa, Alessandro Corsaro, Mario Nizzari, Adriana Bajetto, Alessandra Ratto, Angelo Ferrari, Federica Barbieri, Tullio Florio
Cancer stem cells (CSCs) represent a small subpopulation of cells responsible for tumor formation and progression, drug resistance, tumor recurrence and metastasization. CSCs have been identified in many human tumors including osteosarcoma (OSA). CSC distinctive properties are the expression of stem cell markers, sustained growth, self-renewal and tumorigenicity. Here we report the isolation of stem-like cells from two canine OSA cultures, characterized by self-renewal, evaluated by sphere formation ability, differential marker expression, and in vitro proliferation when cultured in a medium containing EGF and bFGF...
May 2016: Japanese Journal of Veterinary Research