journal
https://read.qxmd.com/read/38478017/sm-protein-sly1-and-a-snare-habc-domain-promote-membrane-fusion-through-multiple-mechanisms
#21
JOURNAL ARTICLE
Mengtong Duan, Guanbin Gao, Ariel Lin, Emma J Mackey, David K Banfield, Alexey J Merz
SM proteins including Sly1 are essential cofactors of SNARE-mediated membrane fusion. Using SNARE and Sly1 mutants and chemically defined in vitro assays, we separate and assess proposed mechanisms through which Sly1 augments fusion: (i) opening the closed conformation of the Qa-SNARE Sed5; (ii) close-range tethering of vesicles to target organelles, mediated by the Sly1-specific regulatory loop; and (iii) nucleation of productive trans-SNARE complexes. We show that all three mechanisms are important and operate in parallel, and that close-range tethering promotes trans-complex assembly when cis-SNARE assembly is a competing process...
June 3, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38477830/n-cadherin-dynamically-regulates-pediatric-glioma-cell-migration-in-complex-environments
#22
JOURNAL ARTICLE
Dayoung Kim, James M Olson, Jonathan A Cooper
Pediatric high-grade gliomas are highly invasive and essentially incurable. Glioma cells migrate between neurons and glia, along axon tracts, and through extracellular matrix surrounding blood vessels and underlying the pia. Mechanisms that allow adaptation to such complex environments are poorly understood. N-cadherin is highly expressed in pediatric gliomas and associated with shorter survival. We found that intercellular homotypic N-cadherin interactions differentially regulate glioma migration according to the microenvironment, stimulating migration on cultured neurons or astrocytes but inhibiting invasion into reconstituted or astrocyte-deposited extracellular matrix...
June 3, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38619450/oma1-clears-traffic-jam-in-tom-tunnel-in-mammals
#23
JOURNAL ARTICLE
Shiori Sekine, Yusuke Sekine
Using an engineered mitochondrial clogger, Krakowczyk et al. (https://doi.org/10.1083/jcb.202306051) identified the OMA1 protease as a critical component that eliminates import failure at the TOM translocase in mammalian cells, providing a novel quality control mechanism that is distinct from those described in yeast.
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38563860/afadin-nectin-forces-its-way-to-the-front
#24
JOURNAL ARTICLE
Michael Sebbagh, Martin A Schwartz
Force transmission at cell-cell junctions critically regulates embryogenesis, tissue homeostasis, and diseases including cancer. The cadherin-catenin linkage has been considered the keystone of junctional force transmission, but new findings challenge this paradigm, arguing instead that the nectin-afadin linkage plays the more important role in mature junctions in the intestinal epithelium.
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38558238/regulation-of-adaptive-growth-decisions-via-phosphorylation-of-the-trappii-complex-in-arabidopsis
#25
JOURNAL ARTICLE
Christian Wiese, Miriam Abele, Benjamin Al, Melina Altmann, Alexander Steiner, Nils Kalbfuß, Alexander Strohmayr, Raksha Ravikumar, Chan Ho Park, Barbara Brunschweiger, Chen Meng, Eva Facher, David W Ehrhardt, Pascal Falter-Braun, Zhi-Yong Wang, Christina Ludwig, Farhah F Assaad
Plants often adapt to adverse or stress conditions via differential growth. The trans-Golgi network (TGN) has been implicated in stress responses, but it is not clear in what capacity it mediates adaptive growth decisions. In this study, we assess the role of the TGN in stress responses by exploring the previously identified interactome of the Transport Protein Particle II (TRAPPII) complex required for TGN structure and function. We identified physical and genetic interactions between AtTRAPPII and shaggy-like kinases (GSK3/AtSKs) and provided in vitro and in vivo evidence that the TRAPPII phosphostatus mediates adaptive responses to abiotic cues...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38558237/p24-family-tango-1-at-the-endoplasmic-reticulum-exit-site-to-organize-cargo-exit
#26
JOURNAL ARTICLE
Kota Saito, Miharu Maeda
The p24 family of proteins have been regarded as cargo receptors for endoplasmic reticulum (ER) to Golgi transport; however, their precise functions have yet to be revealed. In this issue, Pastor-Pareja and colleagues (https://doi.org/10.1083/jcb.202309045) show that the interaction of these proteins with Tango1 is critical for their localization at the ER exit site (ERES) and efficient transport of secretory proteins in Drosophila.
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38530280/oma1-protease-eliminates-arrested-protein-import-intermediates-upon-mitochondrial-depolarization
#27
JOURNAL ARTICLE
Magda Krakowczyk, Anna M Lenkiewicz, Tomasz Sitarz, Dominika Malinska, Mayra Borrero, Ben Hur Marins Mussulini, Vanessa Linke, Andrzej A Szczepankiewicz, Joanna M Biazik, Agata Wydrych, Hanna Nieznanska, Remigiusz A Serwa, Agnieszka Chacinska, Piotr Bragoszewski
Most mitochondrial proteins originate from the cytosol and require transport into the organelle. Such precursor proteins must be unfolded to pass through translocation channels in mitochondrial membranes. Misfolding of transported proteins can result in their arrest and translocation failure. Arrested proteins block further import, disturbing mitochondrial functions and cellular proteostasis. Cellular responses to translocation failure have been defined in yeast. We developed the cell line-based translocase clogging model to discover molecular mechanisms that resolve failed import events in humans...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38517380/tight-junction-membrane-proteins-regulate-the-mechanical-resistance-of-the-apical-junctional-complex
#28
JOURNAL ARTICLE
Thanh Phuong Nguyen, Tetsuhisa Otani, Motosuke Tsutsumi, Noriyuki Kinoshita, Sachiko Fujiwara, Tomomi Nemoto, Toshihiko Fujimori, Mikio Furuse
Epithelia must be able to resist mechanical force to preserve tissue integrity. While intercellular junctions are known to be important for the mechanical resistance of epithelia, the roles of tight junctions (TJs) remain to be established. We previously demonstrated that epithelial cells devoid of the TJ membrane proteins claudins and JAM-A completely lack TJs and exhibit focal breakages of their apical junctions. Here, we demonstrate that apical junctions fracture when claudin/JAM-A-deficient cells undergo spontaneous cell stretching...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38517379/the-emerging-roles-of-non-canonical-ubiquitination-in-proteostasis-and-beyond
#29
JOURNAL ARTICLE
Yoshino Akizuki, Stephanie Kaypee, Fumiaki Ohtake, Fumiyo Ikeda
Ubiquitin regulates various cellular functions by posttranslationally modifying substrates with diverse ubiquitin codes. Recent discoveries of new ubiquitin chain topologies, types of bonds, and non-protein substrates have substantially expanded the complexity of the ubiquitin code. Here, we describe the ubiquitin system covering the basic principles and recent discoveries related to mechanisms, technologies, and biological importance.
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38506728/crosstalk-within-and-beyond-the-polycomb-repressive-system
#30
JOURNAL ARTICLE
Tianyi Hideyuki Shi, Hiroki Sugishita, Yukiko Gotoh
The development of multicellular organisms depends on spatiotemporally controlled differentiation of numerous cell types and their maintenance. To generate such diversity based on the invariant genetic information stored in DNA, epigenetic mechanisms, which are heritable changes in gene function that do not involve alterations to the underlying DNA sequence, are required to establish and maintain unique gene expression programs. Polycomb repressive complexes represent a paradigm of epigenetic regulation of developmentally regulated genes, and the roles of these complexes as well as the epigenetic marks they deposit, namely H3K27me3 and H2AK119ub, have been extensively studied...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38497895/the-phospholipids-cardiolipin-and-phosphatidylethanolamine-differentially-regulate-mdc-biogenesis
#31
JOURNAL ARTICLE
Tianyao Xiao, Alyssa M English, Zachary N Wilson, J Alan Maschek, James E Cox, Adam L Hughes
Cells utilize multiple mechanisms to maintain mitochondrial homeostasis. We recently characterized a pathway that remodels mitochondria in response to metabolic alterations and protein overload stress. This remodeling occurs via the formation of large membranous structures from the mitochondrial outer membrane called mitochondrial-derived compartments (MDCs), which are eventually released from mitochondria and degraded. Here, we conducted a microscopy-based screen in budding yeast to identify factors that regulate MDC formation...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38477922/laura-feltri-of-schwann-cells-matrix-and-family
#32
JOURNAL ARTICLE
Stefano C Previtali, Carla Taveggia
No abstract text is available yet for this article.
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38470363/polarized-localization-of-kinesin-1-and-ric-7-drives-axonal-mitochondria-anterograde-transport
#33
JOURNAL ARTICLE
Youjun Wu, Chen Ding, Behrang Sharif, Alexis Weinreb, Grace Swaim, Hongyan Hao, Shaul Yogev, Shigeki Watanabe, Marc Hammarlund
Mitochondria transport is crucial for axonal mitochondria distribution and is mediated by kinesin-1-based anterograde and dynein-based retrograde motor complexes. While Miro and Milton/TRAK were identified as key adaptors between mitochondria and kinesin-1, recent studies suggest the presence of additional mechanisms. In C. elegans, ric-7 is the only single gene described so far, other than kinesin-1, that is absolutely required for axonal mitochondria localization. Using CRISPR engineering in C. elegans, we find that Miro is important but is not essential for anterograde traffic, whereas it is required for retrograde traffic...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38470362/p24-tango1-interactions-ensure-er-golgi-interface-stability-and-efficient-transport
#34
JOURNAL ARTICLE
Ke Yang, Zhi Feng, José Carlos Pastor-Pareja
The eukaryotic p24 family, consisting of α-, β-, γ- and δ-p24 subfamilies, has long been known to be involved in regulating secretion. Despite increasing interest in these proteins, fundamental questions remain about their role. Here, we systematically investigated Drosophila p24 proteins. We discovered that members of all four p24 subfamilies are required for general secretion and that their localizations between ER exit site (ERES) and Golgi are interdependent in an α→βδ→γ sequence...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38451221/dux4-induced-hsatii-transcription-causes-kdm2a-b-prc1-nuclear-foci-and-impairs-dna-damage-response
#35
JOURNAL ARTICLE
Tessa Arends, Hiroshi Tsuchida, Richard O Adeyemi, Stephen J Tapscott
Polycomb repressive complexes regulate developmental gene programs, promote DNA damage repair, and mediate pericentromeric satellite repeat repression. Expression of pericentromeric satellite repeats has been implicated in several cancers and diseases, including facioscapulohumeral dystrophy (FSHD). Here, we show that DUX4-mediated transcription of HSATII regions causes nuclear foci formation of KDM2A/B-PRC1 complexes, resulting in a global loss of PRC1-mediated monoubiquitination of histone H2A. Loss of PRC1-ubiquitin signaling severely impacts DNA damage response...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38448164/genome-scale-requirements-for-dynein-based-transport-revealed-by-a-high-content-arrayed-crispr-screen
#36
JOURNAL ARTICLE
Chun Hao Wong, Steven W Wingett, Chen Qian, Morag Rose Hunter, J Matthew Taliaferro, Douglas Ross-Thriepland, Simon L Bullock
The microtubule motor dynein plays a key role in cellular organization. However, little is known about how dynein's biosynthesis, assembly, and functional diversity are orchestrated. To address this issue, we have conducted an arrayed CRISPR loss-of-function screen in human cells using the distribution of dynein-tethered peroxisomes and early endosomes as readouts. From a genome-wide gRNA library, 195 validated hits were recovered and parsed into those impacting multiple dynein cargoes and those whose effects are restricted to a subset of cargoes...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38448163/the-derlin-dfm1-couples-retrotranslocation-of-a-folded-protein-domain-to-its-proteasomal-degradation
#37
JOURNAL ARTICLE
Daniela G Vitali, Daniel Fonseca, Pedro Carvalho
Endoplasmic reticulum (ER) proteins are degraded by proteasomes in the cytosol through ER-associated degradation (ERAD). This process involves the retrotranslocation of substrates across the ER membrane, their ubiquitination, and membrane extraction by the Cdc48/Npl4/Ufd1 ATPase complex prior to delivery to proteasomes for degradation. How the presence of a folded luminal domain affects substrate retrotranslocation and this event is coordinated with subsequent ERAD steps remains unknown. Here, using a model substrate with a folded luminal domain, we showed that Cdc48 ATPase activity is sufficient to drive substrate retrotranslocation independently of ERAD membrane components...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38448162/reciprocal-regulation-by-elm1-and-gin4-controls-septin-hourglass-assembly-and-remodeling
#38
JOURNAL ARTICLE
Joseph Marquardt, Xi Chen, Erfei Bi
The septin cytoskeleton is extensively regulated by posttranslational modifications, such as phosphorylation, to achieve the diversity of architectures including rings, hourglasses, and gauzes. While many of the phosphorylation events of septins have been extensively studied in the budding yeast Saccharomyces cerevisiae, the regulation of the kinases involved remains poorly understood. Here, we show that two septin-associated kinases, the LKB1/PAR-4-related kinase Elm1 and the Nim1/PAR-1-related kinase Gin4, regulate each other at two discrete points of the cell cycle...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38407313/dynein-and-dynactin-move-long-range-but-are-delivered-separately-to-the-axon-tip
#39
JOURNAL ARTICLE
Alexander D Fellows, Michaela Bruntraeger, Thomas Burgold, Andrew R Bassett, Andrew P Carter
Axonal transport is essential for neuronal survival. This is driven by microtubule motors including dynein, which transports cargo from the axon tip back to the cell body. This function requires its cofactor dynactin and regulators LIS1 and NDEL1. Due to difficulties imaging dynein at a single-molecule level, it is unclear how this motor and its regulators coordinate transport along the length of the axon. Here, we use a neuron-inducible human stem cell line (NGN2-OPTi-OX) to endogenously tag dynein components and visualize them at a near-single molecule regime...
May 6, 2024: Journal of Cell Biology
https://read.qxmd.com/read/38358349/alternative-mechanisms-of-notch-activation-by-partitioning-into-distinct-endosomal-domains
#40
JOURNAL ARTICLE
Hideyuki Shimizu, Samira Hosseini-Alghaderi, Simon A Woodcock, Martin Baron
Different membrane microdomain compositions provide unique environments that can regulate signaling receptor function. We identify microdomains on the endosome membrane of Drosophila endosomes, enriched in lipid-raft or clathrin/ESCRT-0, which are associated with Notch activation by distinct, ligand-independent mechanisms. Transfer of Notch between microdomains is regulated by Deltex and Suppressor of deltex ubiquitin ligases and is limited by a gate-keeper role for ESCRT complexes. Ubiquitination of Notch by Deltex recruits it to the clathrin/ESCRT-0 microdomain and enhances Notch activation by an ADAM10-independent/TRPML-dependent mechanism...
May 6, 2024: Journal of Cell Biology
journal
journal
23805
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.