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Indian Journal of Microbiology

Ramesh Kumar Saini, Iyyakkannu Sivanesan, Young-Soo Keum
Carotenoids belong to the widespread group of lipophilic tetraterpenoids that play essential roles in plants, microbes, and animals. In photosynthetic microalgae and cyanobacteria, carotenoids are the fundamental components of photosynthesis and protect these organisms from excess radiations, and oxidative stress. Also, polar xanthophyll carotenoids are well known to regulate the biophysical properties of cellular membranes with respect to corresponding changes in temperature. In Zygomycetes fungi, carotenoids-derived trisporoids play crucial roles in early sexual reproduction and mycelial development...
March 2019: Indian Journal of Microbiology
Sanath Kondaveeti, Gunda Mohanakrishna, Jung-Kul Lee, Vipin C Kalia
Methane (CH4 ) is a well-known and abundant feedstock for natural gas, and is readily available from various sources. In thermal plants, the CH4 generated from anthropogenic sources is converted into electrical energy via combustion. Microbial fuel cell (MFC) technology has proven to be an efficient strategy for the biological conversion of a many substrates, including biogas (CH4 ), to electricity. MFC technology uses gaseous substrate along with an enriched and selective microbial consortium. Predominantly, methanotrophs and electrochemically active Geobacter were utilized in a syntrophic association on the anode of an MFC...
March 2019: Indian Journal of Microbiology
Grace N A Charway, Seulki Park, Daeung Yu, Jae-Young Je, Do-Hung Kim, Won-Kyo Jung, Young-Mog Kim
Chitosan-phytochemical conjugates exhibited significant antibacterial effect with minimum inhibitory concentration (MIC) ranging from 128 to 2048 µg/ml against antibiotic-resistant fish pathogenic bacteria such as Edwardseilla tarda , Vibrio harveyi and Photobacterium damselae which were isolated from Korean cultured fish. Furthermore, the MIC values of old-fashioned antibiotics such as erythromycin and oxytertacycline drastically reduced in combination with chitosan-phytochemical conjugates against the fish pathogenic bacteria...
March 2019: Indian Journal of Microbiology
Neelja Singhal, Nambaram Somendro Singh, Shilpa Mohanty, Pooja Singh, Jugsharan Singh Virdi
The enormous health benefits associated with probiotics has resulted in an increased consumption of probiotic supplements. Several factors like regular sub-culturing, storage, unfavourable conditions etc. might compromise the efficacy and/or safety of lactic acid bacteria which are the major components of many probiotic preparations available in the market. The present study evaluated the probiotic characteristics and safety of probiotic bacteria isolated from two preparations available commercially in India...
March 2019: Indian Journal of Microbiology
Yuriy Kuzhyk, Maria Lopatniuk, Andriy Luzhetskyy, Victor Fedorenko, Bohdan Ostash
Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by Streptomyces ghanaensis ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene bldA and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.
March 2019: Indian Journal of Microbiology
Sachin V Otari, Sanjay K S Patel, Sang-Yong Kim, Jung Rim Haw, Vipin C Kalia, In-Won Kim, Jung-Kul Lee
In this study, novel, hollow superparamagnetic copper ferrite (CuFe2 O4 ) nanoparticles (NPs) were synthesized by a low-temperature hydrothermal method. The hollow magnetic spheres were characterized by field emission scanning electron microscopy and high resolution transmission electron microscopy to confirm their morphology and size. The hollow NPs were demonstrated as the support for biological materials by the immobilization of Thermomyces lanuginosus lipase on the inner and outer surfaces of the hollow spheres...
March 2019: Indian Journal of Microbiology
Akhila Krishnaswamy, Natasha Barnes, Nikita P Lotlikar, Samir R Damare
Filamentous fungi are ubiquitous eukaryotes having chitin as a major constituent of the cell wall. Chitin is tough to lyse due to which the intracellular fungal proteins are not readily accessible. The problem is further enhanced when the biomass to be analyzed for protein studies is too little due to the extreme experimental parameters under consideration such as increased or lowered pH, temperature, hydrostatic pressure, nutrients, etc. The method described here is capable of obtaining proteins from minuscule quantities of biomass (~5 mg lyophilized biomass)...
March 2019: Indian Journal of Microbiology
Sanjay K S Patel, Jung-Hoe Kim, Vipin C Kalia, Jung-Kul Lee
To improve the antimicrobial property of chitosan, water-soluble chitosan modified in their quaternary ammonium groups were synthesized. The antimicrobial properties were evaluated against Escherichia coli , Bacillus subtilis , Saccharomyces cerevisiae and Candida tropicalis. The activities increased with increasing cationic charges and the length of the alkyl chain as follows amino-chitosan, dimethylaminoethyl-chitosan, dimethylpropyl amino-chitosan, dimethylamino-1-propyl-chitosan, diethylaminoethyl (DEAE)-chitosan, and quaternized DEAE-chitosan...
March 2019: Indian Journal of Microbiology
Abhijit S Kulkarni, Shreyas V Kumbhare, Dhiraj P Dhotre, Yogesh S Shouche
Human gut microbiome studies are increasing at a rapid pace to understand contributions of the prokaryotic life to the innate workings of their eukaryotic host. Majority of studies focus on the pattern of gut microbial diversity in various diseases, however, understanding the core microbiota of healthy individuals presents a unique opportunity to study the microbial fingerprint in population specific studies. Present study was undertaken to determine the core microbiome of a healthy population and its imputed metabolic role...
March 2019: Indian Journal of Microbiology
Cheng-Chih Tsai, Tzu-Min Lai, You-Miin Hsieh
Urinary tract infections are the most common infectious diseases in babies and the elderly and are often acquired as nosocomial infections. The purpose of the present study was to identify strains of lactic acid bacteria (LAB) which could be used as alternatives to antibiotics for the treatment of urinary tract infections because of their ability to inhibit urinary tract pathogens ( Klebsiella pneumoniae BCRC 10694 and Gardnerella vaginalis BCRC 17040). We screened 370 LAB strains using spent culture supernatants by inhibition zone assay to assess their antimicrobial effects...
March 2019: Indian Journal of Microbiology
Victor Serrano-Solis, Angelica Cardoso Carlos, Vinicius Maracaja-Coutinho, Sávio Torres de Farias
Studies involving miRNAs have opened discussions about their broad participation in viral infections. Regarding the Human gammaherpesvirus 4 or Epstein-Barr virus (EBV), miRNAs are important regulators of viral and cellular gene expression during the infectious process, promoting viral persistence and, in some cases, oncogenic processes. We identified 55 miRNAs of EBV type 2 and inferred the viral mRNA target to self-regulate. This data indicate that gene self-repression is an important strategy for maintenance of the viral latent phase...
March 2019: Indian Journal of Microbiology
Fanbing Chen, Wensong Jin, Huifang Gao, Zewang Guo, Hui Lin, Jiahuan Li, Kaihui Hu, Xiong Guan, Vipin C Kalia, Jung-Kul Lee, Liaoyuan Zhang, Yongyu Li
Bacterial strains from karst landform soil were enriched via chemostat culture in the presence of sodium bicarbonate. Two chemolithotrophic strains were isolated and identified as Serratia marcescens Wy064 and Bacillus sp. Wy065. Both strains could grow using sodium bicarbonate as the sole carbon source. Furthermore, the supplement of the medium with three electron donors (Na2 S, NaNO2 , and Na2 S2 O3 ) improved the growth of both strains. The activities of carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) could be detected in the crude enzyme of strain Wy064, implying that the strain Wy064 might employ Calvin cycle to fix CO2 ...
March 2019: Indian Journal of Microbiology
Mukesh R Jangra, Ritu Batra, Nishat Passricha, Virendra K Sikka
We report here isolation and analysis of PCR amplified phb C gene from Pseudomonas spp. strain phbmbb15-B3. This strain was previously developed from mutations of landfill isolates and found to be an efficient Poly Hydroxy butyrate (PHB) producer. The fragment was cloned into pTZ57R/T cloning vector and then the gene has been sequenced and submitted to GenBank (Accession Number KT933807). The sequence results confirmed the clone to be phbC homologue and the ORF was 910 base pairs long and coded for 303 amino acids, which shared 92-99% amino acid sequence identity with the available bacterial sequences in Gene Bank...
March 2019: Indian Journal of Microbiology
Neeraj Chandra Pant, Ravinder Singh, Aditi Chauhan, Vijaya Gupta, Ravimohan S Mavuduru, Vijay Prabha, Prince Sharma
Staphylococcus warneri , isolated from the cervix of an adult female with unexplained infertility, was found to agglutinate human spermatozoa in vitro leading to their death. A genomic library of S. warneri was generated using pSMART- Escherichia coli vector-host system. Approximately 3500 transformants were screened and four showed sperm agglutinating activity. Sperm agglutinating proteins (SAPs) were partially purified from the positive transformants and were found to agglutinate sperms in vitro. Cloned ORFs in positive transformants were sequenced and ORF finder identified them as endonuclease, accessory secretory protein-Asp1, accessory secretory protein-Asp2 and signal transduction protein...
March 2019: Indian Journal of Microbiology
Debajit Borah, Pronami Gogoi, Dipti Agarwal, Ankita Khataniar
Northeast India is amongst the 34 Biodiversity Hot Spots of the world with large number of unexplored flora and fauna. A total of 74 potential isolates with probiotic properties were isolated from Oecophylla smaragdina , an edible insect used by local tribes as a nutritious medicinal food source out of which, the most potential probiotic candidate was identified as Bacillus sp. PD6 by 16S rRNA sequencing. Cell surface trait analysis shows hydrophobicity and auto-aggregation percentage of 46.2 ± 0.32% and 61...
March 2019: Indian Journal of Microbiology
Sudarshan Maurya, Rashk-E-Eram, S K Naik, J S Choudhary, S Kumar
Trichoderma asperellum (NAIMCC-F-03167) and Hypocrea nigricans (NAIMCC-F-03168) were isolated from the acidic soil of the vicinity of Litchi orchard, Ranchi, Jharkhand and were characterized on the basis of morphological, molecular and biochemical features. Both strains are fast growing, light to dark green, highly sporulative and have ability to cover 90 mm Petri dish within 96 h of inoculation. Biochemcial estimation of both isolates indicated significant cellulase and phosphate solubilisation activity...
March 2019: Indian Journal of Microbiology
Sanath Kondaveeti, Gunda Mohanakrishna, Raviteja Pagolu, In-Won Kim, Vipin C Kalia, Jung-Kul Lee
Algae are autotrophic organisms that are widespread in water bodies. Increased pollution in water bodies leads to eutrophication. However, algae growing in lakes undergoing eutrophication could be utilized towards the generation of added-value bio-electricity using microbial fuel cells (MFCs). In the present study, two methods of electricity generation using raw algae (RA) and RA + acetate (AC) as co-substrate were analyzed in single chamber air cathode MFCs. MFCs supplemented with RA and RA + AC clearly showed higher power density, greater current generation, and improved COD (chemical oxygen demand) removal, which demonstrated the feasibility of using AC as substrate for MFC...
March 2019: Indian Journal of Microbiology
Kuldeep Gupta, Salam Pradeep Singh, Ajay Kumar Manhar, Devabrata Saikia, Nima D Namsa, Bolin Kumar Konwar, Manabendra Mandal
Syzygium cumini L. Skeels (Myretacae family) is a native plant of the Indian subcontinent which has wide socio-economical importance and is well known for its ant diabetic activity. The present study aimed to investigate the antibiofilm activity of purified fraction (EA) from S. cumini leaf extract against P. aeruginosa and S. aureus . The EA did not show any effect on growth of P. aeruginosa and S. aureus at the concentration of 900 µg/ml. At this concentration EA showed biofilm inhibition up to 86 ± 1...
March 2019: Indian Journal of Microbiology
Priti Saxena, Yogesh Joshi, Kartik Rawat, Renu Bisht
Biofilm is a mode of living employed by many pathogenic and environmental microbes to proliferate as multicellular aggregates on inert inanimate or biological substrates. Several microbial diseases are associated with biofilms that pose challenges in treatment with antibiotics targeting individual cells. Bacteria in biofilms secrete exopolymeric substances that contribute to architectural stability and provide a secure niche to inhabiting cells. Quorum sensing (QS) plays essential roles in biofilm development...
March 2019: Indian Journal of Microbiology
Sanjay K S Patel, Jung-Kul Lee, Vipin C Kalia
Theoretical hydrogen (H2 ) yield by dark fermentative route is 12 mol/mol of glucose. Biological H2 production yields of 3.8 mol/mol of glucose by microbes have been reported. Transient gene inactivation in combination with adaptive laboratory evolution strategy has enabled the H2 yield to exceed the stoichiometric production values.
December 2018: Indian Journal of Microbiology
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