Xiao Tan, Laura F Grice, Minh Tran, Onkar Mulay, James Monkman, Tony Blick, Tuan Vo, Ana Clara Almeida, Jarbas da Silva Motta, Karen Fernandes de Moura, Cleber Machado-Souza, Paulo Souza-Fonseca-Guimaraes, Cristina Pellegrino Baena, Lucia de Noronha, Fernanda Simoes Fortes Guimaraes, Hung N Luu, Tingsheng Drennon, Stephen Williams, Jacob Stern, Cedric Uytingco, Liuliu Pan, Andy Nam, Caroline Cooper, Kirsty Short, Gabrielle T Belz, Fernando Souza-Fonseca-Guimaraes, Arutha Kulasinghe, Quan Nguyen
The SARS-CoV-2 (COVID-19) virus has caused a devastating global pandemic of respiratory illness. To understand viral pathogenesis, methods are available for studying dissociated cells in blood, nasal samples, bronchoalveolar lavage fluid and similar, but a robust platform for deep tissue characterization of molecular and cellular responses to virus infection in the lungs is still lacking. We developed an innovative spatial multi-omics platform to investigate COVID-19-infected lung tissues. Five tissue-profiling technologies were combined by a novel computational mapping methodology to comprehensively characterize and compare the transcriptome and targeted proteome of virus infected and uninfected tissues...
August 21, 2023: Immunology