Yi Song, Yingyu Liu, Qianhan Xu, Kam Tong Leung, Loucia Kit Ying Chan, Jacqueline Pui Wah Chung, Chi Chiu Wang, Xiaoyan Chen, Tao Zhang, Gene Chi Wai Man
Endometriosis is a chronic, painful disease whose etiology remains unknown. The development of novel therapies and diagnostic tools for endometriosis has been limited due in part to challenges in studying the disease. Recently, a few reports have shown that immunosuppressive cells, such as myeloid-derived suppressor cell (MDSC), may promote the progression of endometriosis. MDSCs are a heterogeneous group of myeloid cells with potent immunosuppressive and angiogenic properties. Here, in this chapter, we provide a detailed protocol to phenotype MDSC as well as to isolate and assess the functionality from the peritoneal cavity of a mouse model of surgically induced endometriosis...
2024: Methods in Cell Biology