Nasim Maghboli Balasjin, James Maki, Michael Schlappi
Cold stress is an important factor limiting rice production and distribution. Identifying factors that contribute to cold tolerance in rice is of primary importance. While some plant specific genetic factors involved in cold tolerance have been identified, the role of the rice microbiome remains unexplored. In this study, we evaluated the influence of plant growth promoting bacteria (PGPB) with the ability of phosphate solubilization on rice cold tolerance and survival. To reach this goal, inoculated and uninoculated two-week old seedlings were cold stressed and evaluated for survival and other phenotypes such as electrolyte leakage (EL) and necessary elements for cold tolerance...
September 12, 2023: Canadian Journal of Microbiology
Ellen Sykes, Dawn White, Sydney McLaughlin, Ayush Kumar
Salicylic acids have been used in human and veterinary medicine for their anti-pyretic, anti-inflammatory and analgesic properties for centuries. A key role of salicylic acid-immune modulation in response to microbial infection-was first recognized during studies of their botanical origin. The effects of salicylic acid on bacterial physiology are diverse. In many cases, they impose selective pressures leading to development of cross-resistance to antimicrobial compounds. Initial characterization of these interactions was in Escherichia coli, where salicylic acid activates the multiple antibiotic resistance (mar) operon, resulting in decreased antibiotic susceptibility...
September 12, 2023: Canadian Journal of Microbiology
Bianjin Sun, Shudan Lin, Mengmeng Zheng, Beijia Zheng, Liping Mao, Yunfeng Gu, Jiabei Cai, Yiran Dai, Meiqin Zheng, Lou Yongliang
Bacillus cereus endophthalmitis is a devastating eye infection that causes rapid blindness through the release of extracellular tissue-destructive exotoxins. The phagocytic and antibacterial functions of ocular cells are the keys to limiting ocular bacterial infections. In a previous study, we identified a new virulence gene, plcA-2 (different from the original plcA-1 gene), that was strongly associated with the plcA gene of Listeria monocytogenes. This plcA gene had been confirmed to play an important role in phagocytosis...
September 6, 2023: Canadian Journal of Microbiology
Radhey S Gupta
The genus Robertmurraya was created by my group in 2020 to recognize the contributions of Dr. Robert G.E. Murray to the field of prokaryotic taxonomy. This manuscript updates the information regarding this genus. In addition to the seven Robertmurraya species with validly published names, the work presented here shows that two species with effectively published names, " Bacillus yapensis " and " Bacillus dakarensis ", and an uncharacterized Bacillus sp. Y1 are also affiliated with this genus. Based on these results, reclassification of " Bacillus yapensis " as a novel species Robertmurraya yapensis sp...
August 9, 2023: Canadian Journal of Microbiology
Arjan de Groot, Laurence Blanchard
Deinococcus murrayi is a bacterium isolated from hot springs in Portugal, and named after Dr. Robert G. E. Murray in recognition of his research on the genus Deinococcus. Like other Deinococcus species, D. murrayi is extremely resistant to ionizing radiation. Repair of massive DNA damage and limitation of oxidative protein damage are two important factors contributing to the robustness of Deinococcus bacteria. Here, we identify, amongst others, the DNA repair and oxidative stress defense proteins in D. murrayi, and highlight special features of D...
August 8, 2023: Canadian Journal of Microbiology
Xiaolei Su, Tingzheng Fang, Ling Fang, Dapeng Wang, Xuege Jiang, Changting Liu, Honglei Zhang, Rui Guo, Junfeng Wang
In our study, Bacillus subtilis was disposed to a simulated microgravity (SMG) environment in high-aspect ratio rotating-wall vessel bioreactors for 14 days, while the control group was disposed to the same bioreactors in a normal gravity (NG) environment for 14 days. The B. subtilis strain exposed to the SMG (labeled BSS) showed an enhanced growth ability, increased biofilm formation ability, increased sensitivity to ampicillin sulbactam and cefotaxime, and some metabolic alterations compared with the B...
July 18, 2023: Canadian Journal of Microbiology
Juan Lan, Ting Liu, Liyun Miao, Ting Pei, Zhe Gan, Aihua Lin, Hong Geng, Peng Zhang
Endophytes confer fitness advantages to host plants. However, the ecological communities of endophytic fungi in the different tissues (rhizomes, stems, and leaves) of Paris polyphylla and the relationship of their endophytic fungi with polyphyllin levels remain unclear. In this study, the community diversity and differences of endophytic fungi in the rhizomes, stems, and leaves of P. polyphylla var. yunnanensis were investigated, and a comprehensively diverse community of endophytic fungi was represented, including 50 genera, 44 families, 30 orders, 12 classes, and 5 phyla...
July 10, 2023: Canadian Journal of Microbiology
Jin Wu, Xianyong Li, Rui Kong, Jiankun Wang, Xiaoling Wang
The surface morphology of mature biofilms is heterogeneous and can be divided into concentric rings wrinkles (I), labyrinthine networks wrinkles (II), radial ridges wrinkles(III) and branches wrinkles (IV), according to surface wrinkle structure and distribution characteristics. Due to the wrinkle structures, channels are formed between the biofilm and substrate and transport nutrients, water, metabolic products, etc. We find that expansion rate variations of biofilms growing on substrates with high and low agar concentration (1...
June 28, 2023: Canadian Journal of Microbiology
Srajana Nayak, D'Almeida Aanice, Dsouza Andria, Archana Pai, Biswajit Maiti
Acinetobacter baumannii is an opportunistic pathogen known for causing hospital-acquired infections. The natural habitat includes soil, water, sewage, and drains, but it is also detected in infected individuals' blood, pus, and respiratory pathways. Due to its resilient nature, it is known to be a causative agent for outbreaks. Therefore, it is crucial to understand the genetic similarity between clinical and environmental isolates. The study aimed to find the genetic relationships between clinical and environmental isolates using PCR-based typing methods such as enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR), random amplified polymorphic DNA (RAPD), and repetitive extragenic palindromic sequence-based PCR (Rep-PCR)...
June 26, 2023: Canadian Journal of Microbiology
Sipriyadi Sipriyadi, Yeni Khairina, Masrukhin Masrukhin, Adi Yulandi, Risky Hadi Wibowo, Dhiatama Tauhida Nisa
The rhizosphere is a narrow soil area directly affected by plant root exudates. Microbes inhabiting the rhizosphere have been widely studied for their beneficial effects on plant nutrition, growth, and disease prevention. Many factors affect the rhizosphere microbial composition, including plant pathogen infection. Here, we analyzed the bacterial community structure in the rhizosphere of fungi-infected Amorphophallus titanum. Soil samples were collected from rhizosphere and non-rhizosphere areas of fungi-infected A...
June 26, 2023: Canadian Journal of Microbiology
Dianbin Liu, Haohan Huang, Jinzhi Han, Qiong Wu, Yaoyao Xiang, Yan Liu, Yanxia Wei
Bifidobacterium and Lactobacillus are known to be common members of the human intestinal microbiota, which plays important roles in maintaining the homeostasis of host gut microenvironment. Several bifidobacterial and lactobacilli strains have been used as probiotics for health benefits. The exopolysaccharides (EPS) produced by strains from Bifidobacterium and Lactobacillus are considered as beneficial traits mediating these beneficial effects. In this study, 21 strains belong to Bifidobacterium and Lactobacillus were isolated from healthy infants' stool and were screened for EPS-producing ability...
June 23, 2023: Canadian Journal of Microbiology
Annabelle Mathieu-Denoncourt, Marylise Duperthuy
The Type VI Secretion System (T6SS) is used by bacteria for virulence, resistance to grazing and competition with other bacteria. We previously demonstrated that the role of the T6SS in interbacterial competition and in resistance to grazing is enhanced in Vibrio cholerae in the presence of subinhibitory concentrations of polymyxin B. Here, we performed a global quantitative proteomic analysis and a targeted transcriptomic analysis of the T6SS known regulators in V. cholerae grown with and without polymyxin B...
June 21, 2023: Canadian Journal of Microbiology
Tam Thanh Tran, Marlena Cole, Emily Tomas, Andrew Scott, Edward Topp
The goal of this study was to (a) determine the minimum selection concentrations of tetracycline family antibiotics necessary to maintain plasmids carrying tetracycline-resistant genes and (b) correlate these results to environmental hotspot concentrations reported in previous studies. This study used two plasmids (pT295A and pT413A) originating from dairy manure in a surrogate Escherichia coli host CV601. The minimum selection concentrations of antibiotics tested in nutrient-rich medium were determined as follows: 0...
June 2, 2023: Canadian Journal of Microbiology
Michael Daly
The Deinococcaceae family exhibits exceptional radiation resistance and possesses all the necessary traits for surviving in radiation-exposed environments. Their survival strategy involves the coupling of metabolic and DNA repair functions, resulting in an extraordinarily efficient homologous repair of DNA double-strand breaks (DSBs) caused by radiation or desiccation. The keys to their survival lie in the hyperaccumulation of manganous (Mn2+)-metabolite antioxidants that protect their DNA repair proteins under extreme oxidative stress, and the persistent structural linkage by Holliday junctions of their multiple genome copies per cell that facilitates DSB repair...
June 2, 2023: Canadian Journal of Microbiology
Wen-Jing Pan, Li-Yun Miao, Shi-Peng Fan, Pin-Wei Lv, Ai-Hua Lin, Hong Geng, Fa-Jun Song, Peng Zhang
Endophytic bacteria play crucial roles in the growth and bioactive compound synthesis of host plants. In this study, the composition and diversity of endophytic bacteria in the roots, stems, and leaves from 3-year-old artificially cultivated Huperzia serrata were investigated using Illumina HiSeq sequencing technology. Total effective reads were assigned to 936 operational taxonomic units (OTUs), belonging to 12 phyla and 289 genera. A total of 28, 3, and 2 OTUs were exclusive to the roots, stems, and leaves, respectively...
May 30, 2023: Canadian Journal of Microbiology
Mohd Faidz Mohamad Shahimin, Tariq Siddique
Anaerobic microorganisms in Canada Natural Upgrading Limited (CNUL) fluid fine tailings (FFT) are sustained by residual solvent hydrocarbons. Although FFT are methanogenic in nature, sulfate-reducing microorganisms represent a significant portion of FFT bacterial community. In this study, we examined biodegradation of three iso-alkanes (2-methylbutane, 2-methylpentane, 3-methylpentane), representing major iso-alkanes in paraffinic solvent, in CNUL FFT under sulfate-reducing conditions. During ~1100 d of incubation, only 2-methylpentane was degraded partially whereas 2-methylbutane and 3-methylpentane were not degraded...
May 26, 2023: Canadian Journal of Microbiology
Zhiyu Pan, Juan Guo, Yilin Zhong, Lvyuan Fan, Yubin Su
Antibiotic overuse and misuse have promoted the emergence and spread of antibiotic-resistant bacteria. Increasing bacterial resistance to antibiotics is a major healthcare problem, necessitating elucidation of antibiotic resistance mechanisms. In this study, we explored the mechanism of gentamicin resistance by comparing the transcriptomes of antibiotic-sensitive and -resistant Escherichia coli. A total of 410 differentially expressed genes were identified, of which 233 (56.83%) were up-regulated and 177 (43...
May 24, 2023: Canadian Journal of Microbiology
Qijing Ren, Yimeng Zhang, Jizhou Duan, Wangqiang Li, Xucheng Dong, Yingying Pei, Ruiyong Zhang, Qingjun Zhu
In this paper, high-throughput sequencing technology was adopted to visualize the microbial communities on the surfaces of two types of carbon steels immersed in Nansha waters. The results showed that different microbial communities were formed on different carbon steel surfaces, in which the genus with the highest abundance on the surface of Q235 was Escherichia-Shigella, while anaerobic Desulfovibrio on the surface of 921a was the most, and the dominant genus varied with the depth of the rust layer. In addition, the distribution of sulfate-reducing bacteria on the surface of Q235 submerged in Sanya waters conducted the environmental factors correlation analysis with the sulfate-reducing bacteria's distribution in Nansha waters...
May 8, 2023: Canadian Journal of Microbiology
Ronald S Flannagan
No abstract text is available yet for this article.
April 28, 2023: Canadian Journal of Microbiology
Xing-Guang Xie, Zhen-Zhen Zhang, Ling Chen, Qian-Liang Ming, Ke-Xin Sheng, Xi Chen, Khalid Rahman, Kun-Miao Feng, Juan Su, Ting Han
Using endophytic fungal elicitors to increase the accumulation of valuable secondary metabolites in plant tissue culture is an effective biotechnology strategy. In this study, a collection of 56 strains of endophytic fungi were isolated from different organs of cultivated Panax ginseng, of which seven strains can be symbiotically co-cultured with the hairy roots of P. ginseng. Further experiments observed that strain 3R-2, identified as endophytic fungus Schizophyllum commune, can not only infect hairy roots, but also promote the accumulation of specific ginsenosides...
April 21, 2023: Canadian Journal of Microbiology
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