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Bulletin of Experimental Biology and Medicine

E G Skurikhin, A V Pakhomova, A A Epanchintsev, O V Stronin, N N Ermakova, O V Pershina, L A Ermolaeva, V A Krupin, A I Kudryashova, V V Zhdanov, A M Dygai
The title of the article should read:"Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of the Pegylated Form of Glucagon-Like Peptide 1".
March 11, 2019: Bulletin of Experimental Biology and Medicine
N D Bunyatyan, N A Oborotova, L L Nikolaeva, N S Saprykina, L M Borisova, M P Kiseleva, A B Prokof'ev
We performed an in vivo comparative study of activity of three substances of the nitrosourea group produced in Russia. All substances demonstrated high antitumor activity against various solid and leukemic tumors. Aranosa significantly enhanced life duration in mice with leukemia (by 65-194%) and inhibited the growth of solid tumors (by 49-99.6%). Lisomustine and ormustine showed higher activity than aranose. Single administration of lisomustine increased life span of mice (by 22-114%) and resulted in cure of all animals in four models: lymphoblastic leukemia L-1210, lymphocytic leukemia P-388, Lewis lung carcinoma, and cervical cancer RShM-5...
February 27, 2019: Bulletin of Experimental Biology and Medicine
E S Zubkova, I B Beloglazova, E G Evtushenko, A T Kopylov, E K Shevchenko, K V Dergilev, E I Ratner, E V Parfenova, M Yu Men'shikov
Mesenchymal stromal cells from rat adipose tissue were transduced with adeno-associated viral (AAV) vector encoding stem cell factor SCF that stimulates proliferation of cardiac c-kit+ cells and improved cardiac function and survival of animals after myocardial infarction. Extracellular vesicles isolated from the medium conditioned by mesenchymal stromal cells by ultracentrifugation were characterized by Western blotting, transmission electron microscopy, nanoparticle tracking analysis, immunostaining, and mass spectrometry analysis...
February 22, 2019: Bulletin of Experimental Biology and Medicine
Yu A Romanov, N E Volgina, V V Vtorushina, A Yu Romanov, T N Dugina, N V Kabaeva, G T Sukhikh
Production of cytokines and growth factors by cultured human umbilical cord tissue- and bone marrow-derived multipotent mesenchymal stromal cells was measured by multiplex analysis. In most cases, the concentrations of bioactive factors in the culture medium conditioned by umbilical cord-derived cells was ten- to hundred-times higher than in the medium conditioned by bone marrow-derived cells. These results suggest that both multipotent mesenchymal stromal cells from the umbilical cord and cell-free products can have more pronounced therapeutic effect in comparison with mesenchymal stromal cells obtained from "adult" sources...
February 22, 2019: Bulletin of Experimental Biology and Medicine
V K Spiridonov, Z S Tolochko, T A Korolenko
The development of arterial hypertension in male Wistar rats with fructose-induced metabolic syndrome (12.5% of fructose solution as the only drinking source for 10 weeks) along with impaired glucose tolerance and increased serum concentration of triglycerides and LPO products caused a decrease in the content of serum blood calcitonin gene-related peptide (CGRP). Low-frequency transcutaneous electrical nerve stimulation (1 mA, 2 Hz, 10 min daily for 2 weeks) performed in 8 weeks after the beginning of fructose treatment reduced systolic BP and serum concentration of triglycerides and LPO produces and improved glucose tolerance...
February 21, 2019: Bulletin of Experimental Biology and Medicine
V A Mikhailova, D O Bazhenov, L P Viazmina, A O Agnaeva, O N Bespalova, S A Sel'kov, D I Sokolov
We evaluated cytotoxic activity of peripheral blood NK cells towards trophoblast cells. NK cells either isolated or in the composition of mononuclear cell fraction, caused death of trophoblast cells. In women with physiological pregnancy, the cytotoxic effect of NK cells present in mononuclear cell fraction preincubated with IL-2 was lower than in nonpregnant women, who had never been pregnant previously, and in fertile women. Cytotoxic activity of isolated NK cells preincubated with IL-2 in fertile women was lower than in nonpregnant women, who had never been pregnant previously...
February 20, 2019: Bulletin of Experimental Biology and Medicine
E A Cherkashova, V V Burunova, T B Bukharova, D D Namestnikova, I L Gubskii, D I Salikhova, E V Galitsina, G E Leonov, V P Chekhonin, L V Gubskii, S L Kisevev, D V Goldstein, K N Yarygin
We compared the effects of placental mesenchymal stromal cells and neural progenitor cells derived from induced human pluripotent cells after their intravenous administration to rats in 24 h after transitory occlusion of the middle cerebral artery. The therapeutic effects were evaluated by the dynamics of animal survival, body weight, neurological deficit, and the volume of infarction focus in 7, 14, 30, and 60 days after surgery. Intravenous injection of neural progenitor cells produced a therapeutic effect on the course of experimental ischemic stroke by increasing animal survival in the most acute period and accelerating compensation of neurological deficit and body weight recovery...
February 20, 2019: Bulletin of Experimental Biology and Medicine
L F Zainullina, T V Ivanova, R U Ostrovskaya, T A Gudasheva, Yu V Vakhitova, S B Seredenin
Effects of Noopept (N-phenylacetyl-L-prolylglycine ethyl ester) on the relative level of proliferation marker Ki-67 and cell cycle parameters were studied in HEK293 and SH-SY5Y cell lines. The previously established multifactorial mechanism of action of the drug includes enhancement of neurotrophin NGF and BDNF expression and increase in HIF-1 activity. The possible mitogenic action of Noopept was estimated by its effect on cell proliferation. Noopept did not affect cell distribution over G1, S, G2 cell cycle phases and the relative level of proliferation marker Ki-67 in the cell lines under study...
February 20, 2019: Bulletin of Experimental Biology and Medicine
N V Kirbaeva, V S Evstratova, N A Riger, A Yu Abramova, S S Pertsov, A V Vasil'ev
Changes in the blood cytokine profile in rats with various parameters of behavior were studied under conditions of 5-day starvation not followed by the recovery period or with subsequent normalization of food intake. Metabolic stress had no effect on the content of most cytokines. The concentration of pro- and anti-inflammatory cytokines in blood plasma of rats was shown to increase significantly by the end of a 5-day recovery period after food deprivation. These changes in behaviorally passive specimens were more pronounced than in active animals...
February 20, 2019: Bulletin of Experimental Biology and Medicine
L B Kim, V A Shkurupy, A N Putyatina
Correlations between extracellular matrix components in mouse liver revealed the pathogenetically determined dynamic structure of these interrelations (some correlations appeared while others disappeared) at various terms of BCG-induced granulomatosis. The correlations between the studied parameters were strong during the first (postinfection days 3-10) and the second (postinfection days 60-90) periods but became moderate during the third period manifested by chronic inflammation (postinjection day 180). The greatest number of correlations was observed at the beginning of the stabilization period (postinjection day 60)...
February 20, 2019: Bulletin of Experimental Biology and Medicine
L D Luk'yanova, Yu I Kirova, E L Germanova
We performed a complex study of the dependence of immediate reaction of catalytic subunits in mitochondrial enzymes (NDUFV2, SDHA, Cyt b, COX1, and ATP5A) in rat cerebral cortex (the most hypoxia-sensitive tissue) on the severity and duration of hypoxia in vivo and the role of individual resistance of rats to oxygen deficiency in this process. Three types of responses to hypoxia were revealed. The immediate response of mitochondria to oxygen deficiency appeared after its drop by 30-33% relatively to normal atmosphere level...
February 20, 2019: Bulletin of Experimental Biology and Medicine
V I Kirpatovskii, G D Efremov, E V Frolova, L V Kudryavtseva
The abdominal type of cryptorchism was modeled on random-bred albino rats by replacing both testes from the scrotum into the abdominal cavity for 3 weeks; thereupon they were manipulated into the scrotum. In control rats, no additional surgery was performed. In experimental rats, the testicular tissue obtained from 1-2-day rat pups was transplanted under testicular tunica albuginea. Prior to orchiopexy, the weight of testes decreased by 62.5-64.1%. In 6 month after the surgery, it increased by 36.1% in the control group, whereas in experimental rats the weight of testes elevated by 123...
February 20, 2019: Bulletin of Experimental Biology and Medicine
A A Tikhonov, E N Savvateeva, M A Chernichenko, V V Maslennikov, D V Sidorov, A Yu Rubina, N E Kushlinskii
We propose an approach that allows simultaneous determination of the levels of M and G isotypes of antibodies to the panel of glycans using microarrays. The level of IgG antibodies to 3'-O-su-Lea glycan detects patients with colorectal cancer with a sensitivity of 69.77% and specificity of 62.75%. The percentage of correctly classified colorectal cancer patients with the use of a combination of two markers IgM antibodies to glycans 3'-sialyl-TF and 3'-O-su-Lea is as high as 74.23%. The levels of IgM antibodies to 3'-O-su-Lea glycan differ significantly in patients with and without regional metastases...
February 20, 2019: Bulletin of Experimental Biology and Medicine
Е А Kurbatova, I V Yakovleva, E А Akhmatova, Е V Sukhova, D V Yashunsky, Yu E Tsvetkov, N E Nifantiev
We studied carbohydrate specificity and isotypes of antibodies to BSA-conjugated tetrasaccharide, a repeating unit of the capsular polysaccharide of Streptococcus pneumoniae serotype 14, in mouse polyclonal sera and hybridoma-synthesized products. Natural IgM antibodies to the tetrasaccharide containing epitopes similar to surface carbohydrate structures of mammalian and human cells in low titers were determined in native mouse serum by ELISA using biotinylated tetrasaccharide and synthetic capsular polysaccharide as the solid-phase antigens...
February 20, 2019: Bulletin of Experimental Biology and Medicine
S G Krylova, T N Povet'eva, E P Zueva, N I Suslov, E N Amosova, T G Razina, K A Lopatina, O Yu Rybalkina, Yu V Nesterova, O G Afanas'eva, E A Kiseleva, S V Sysolyatin, D A Kulagina, V V Zhdanov
Pronounced analgesic activity of the innovative compound 4-(3,4-dibromthiophencarbonyl)-2,6,8,12-tetraacethyl-2,4,6,8,10,12hexaazatetracyclo[5,5,0,03, 11 ,05, 9 ] dodecane (thiowurtzine) was observed in the thermal nociceptive hot plate test and in the acute visceral and somatic deep pain model (acetic acid writhing test). In these experimental models, naloxone-sensitive thiowurtzine-induced analgesia was revealed. The absence of tropism to peripheral opioid receptors in the acetic acid writhing test was demonstrated using naloxone methiodide...
February 20, 2019: Bulletin of Experimental Biology and Medicine
G N Zyuz'kov, V V Zhdanov, E V Udut, L A Miroshnichenko, T Yu Polyakova, L A Stavrova, V V Udut
The study focuses on the development of principally novel priority-oriented healthcare strategy - targeted therapy in regenerative medicine known as Strategy of Pharmacological Control over Intracellular Signal Transduction in Regeneration-Competent Cells. It implies selective action of promising drugs on specific key elements in the signaling cascade responsible for functional activity of various progenitor cells (including stem cells) and elements of tissue microenvironment. The results of pioneer studies are described that were aimed on revealing the peculiarities in signal transduction and the role of distinct signaling molecules (the potential targets) in the control of cell cycle and other functions of progenitor elements and regulatory cells of different types...
February 20, 2019: Bulletin of Experimental Biology and Medicine
Yu F Gorskaya, A I Tukhvatulin, A Sh Dzharullaeva, V G Nesterenko
In 24 h after combined administration of ligands of NOD2 (muramyl dipeptide) and TLR4 (LPS) receptors to CBA mice, a synergistic increase (by 10 times compared to the intact control) in cloning efficiency and content of multipotent stromal cells was observed in the bone marrow in comparison with the total effects of their individual administration (by 2.1 and 4.1 times, respectively). A similar effect was also observed in the peritoneal exudate. When ligands were administered simultaneously, the concentration of osteogenic multipotent stromal cells in the bone marrow decreased to a greater extent than in case of individual injections of the ligands, but did not drop below 7% of the control, which is apparently indicative of a decline threshold...
February 20, 2019: Bulletin of Experimental Biology and Medicine
M S Tverskaya, L V Gankovskaya, V V Sukhoparova, A O Virganskii, R V Gorenkov
The effects of a natural complex of cytokines IL-1, IL-2, IL-6, TNF, MIF, and GTFβ on myocardial blood flow were studied under control conditions and during acute experimental aortal stenosis. Systemic administration of the cytokine complex under control conditions led to moderate impairment of the blood flow in the myocardium associated with plethora and perivascular edema. The number of functioning vessels in the myocardium significantly increased under these conditions, which reflected enhancement of the coronary blood flow...
February 20, 2019: Bulletin of Experimental Biology and Medicine
E I Shakhmatova, D V Golosova, Yu V Natochin
We developed an approach for quantitative assay of injected vasopressin in urine samples by ELISA under conditions of physiological suppression of hormone secretion from the neurohypophysis into the blood. In experiments on unanesthetized rats, water load (5 ml/100 g body weight) almost completely blocked secretion of arginine-vasopressin. Injection of arginine-vasopressin in a dose of 0.1 nmol/100 g body weight after water load enhanced reabsorption of solute-free water and renal excretion of Na+ , K+ , and Mg2+ by 13...
February 20, 2019: Bulletin of Experimental Biology and Medicine
M A Nakvasina, L I Popova, O V Lidokhova, V G Artyukhov
The effects of ROS on functional properties (cytotoxic activity, antibody-producing activity, TNFα synthesis, and free cytosol calcium level), membrane structure (by expression of some surface markers), and apoptosis of lymphocytic cells were estimated in the peripheral blood of healthy volunteers. 1 O2 , [Formula: see text], OH• , and H2 O2 mostly suppressed cytotoxic activity of lymphocytes against Ehrlich ascites carcinoma cells and inhibited IgG synthesis and expression of receptors and surface markers (Fc receptors, CD3, CD19, and CD56)...
February 20, 2019: Bulletin of Experimental Biology and Medicine
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