journal
Journals Bulletin of Experimental Biolo...

Bulletin of Experimental Biology and Medicine

https://read.qxmd.com/read/38733483/mesenchymal-stem-cells-from-the-deciduous-tooth-pulp-lose-their-ability-to-suppress-the-differentiation-of-dendritic-cells-during-long-term-culturing
#1
JOURNAL ARTICLE
A Yu Lupatov, I V Vakhrushev, R Yu Saryglar, K N Yarygin
A culture of cells expressing markers of mesenchymal stem cells (MSC) (CD73, CD90, CD44, CD29, and CD49b), but not hematopoietic cell markers, and capable of multilineage differentiation was isolated from the deciduous tooth pulp. Co-culturing with immature dendritic cells in the presence of LPS did not reveal an ability of the MSC to suppress the maturation of dendritic cells. On the contrary, co-culturing of MSC with monocytes in the presence of granulocyte-macrophage CSF and IL-4 led to complete suppression of monocyte differentiation into dendritic cells...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38733482/estimation-of-the-ischemic-lesion-in-the-experimental-stroke-studies-using-magnetic-resonance-imaging-review
#2
JOURNAL ARTICLE
D D Namestnikova, E A Cherkashova, I S Gumin, V P Chekhonin, K N Yarygin, I L Gubskiy
In translational animal study aimed at evaluation of the effectiveness of innovative methods for treating cerebral stroke, including regenerative cell technologies, of particular importance is evaluation of the dynamics of changes in the volume of the cerebral infarction in response to therapy. Among the methods for assessing the focus of infarction, MRI is the most effective and convenient tool for use in preclinical studies. This review provides a description of MR pulse sequences used to visualize cerebral ischemia at various stages of its development, and a detailed description of the MR semiotics of cerebral infarction...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38733481/experimental-approaches-to-scar-modeling-through-mechanical-skin-damage-and-chemical-burn-in-sprague-dawley-rats
#3
JOURNAL ARTICLE
Yu A Palikova, V A Palikov, V A Kazakov, N A Borozdina, K V Khmara, S G Semushina, I A Dyachenko
Creating of a scar model in laboratory animals is the most acceptable option for the preclinical search of scar treatment. However, due to high skin regeneration rate in laboratory rodents, creating an optimal animal model of scar formation is a challenge. Here we describe five methods for modeling a scar tissue in rats that we have tested. These methods allowed achieving different histopathological features and different stages of skin scar formation.
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38733480/buccal-mesenchymal-stromal-cells-as-a-source-of-osseointegration-of-titanium-implants
#4
JOURNAL ARTICLE
M A Surovtseva, I I Kim, N A Bondarenko, S V Ostapets, M N Drovosekov, O S Kosareva, O V Poveshchenko
We studied the interaction of human buccal mesenchymal stem cells (MSCs) and osteoblasts differentiated from them with the surface of titanium samples. MSCs were isolated by enzymatic method from buccal fat pads. The obtained cell culture was presented by MSCs, which was confirmed by flow cytometry and differentiation into adipocytes and osteoblasts. Culturing of buccal MSCs on titanium samples was accompanied by an increase in the number of cells for 15 days and the formation of a developed network of F-actin fibers in the cells...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38733479/effect-of-pyridoxine-derivative-b6no-on-transcription-factor-nrf2-activity-and-cytotoxic-properties-of-doxorubicin-in-vitro
#5
JOURNAL ARTICLE
A A Balakina, V I Amozova, T R Prikhodchenko, T S Stupina, D V Mishchenko
The effect of a new pyridoxine derivative B6NO on doxorubicin cytotoxicity and Nrf2-dependent cellular processes in vitro was studied. Antioxidant B6NO enhances the cytotoxic effect of doxorubicin on tumor cells, which is associated with G2/M cell division arrest and an increase in activity of proapoptotic enzyme caspase-3. The antioxidant promotes intracellular accumulation and nuclear translocation of Nrf2 transcription factor in non-tumor and tumor cells. In non-tumor cells, B6NO increases the expression of antioxidant system proteins and reduces ROS generation in the presence of doxorubicin...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38733478/comparative-molecular-and-biological-characteristic-of-the-systemic-inflammatory-response-in-adult-and-old-male-wistar-rats-with-different-resistance-to-hypoxia
#6
JOURNAL ARTICLE
D Sh Dzhalilova, M V Silina, A M Kosyreva, I S Tsvetkov, O V Makarova
Morphological, molecular, and biological features of the systemic inflammatory response induced by LPS administration were assessed in adult and old male Wistar rats with high and low resistance to hypoxia. In 6 h after LPS administration, mRNA expression levels of Hif1a, Vegf, Nfkb, and level of IL-1β protein in old rats were higher than in adult rats regardless of hypoxia tolerance. The morphometric study showed that the number of neutrophils in the interalveolar septa of the lungs was significantly higher in low-resistant adult and old rats 6 h after LPS administration...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38733477/morphological-and-neurological-impairments-induced-by-chronic-administration-of-dimethyl-sulfoxide-in-mice
#7
JOURNAL ARTICLE
N V Kudryashov, N B Sviridkina, A A Gorbunov, S E Mironov, D A Tikhonov, A A Nedorubov, V P Fisenko
We studied the influence of DMSO administered ad libitum with drinking water in concentrations of 0.01, 0.1, and 1% for 4 and 6 weeks on pain sensitivity, motor coordination, and myelin content in the corpus callosum of C57BL/6 mice. After 6-week administration, DMSO in all studied concentrations decreased myelin content in the corpus callosum. Moreover, 4-week administration of 0.1% DMSO and 6-week administration of 1% DMSO increased the latency to fall in the rotarod test by 3.1 (p<0.05) and 5.1 (p<0...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38730109/bismuth-nanoparticles-increase-effectiveness-of-proton-therapy-of-ehrlich-carcinoma
#8
JOURNAL ARTICLE
M V Filimonova, O V Soldatova, A A Shitova, A S Filimonov, V A Rybachuk, A O Kosachenko, K A Nikolaev, G A Demyashkin, A A Popov, I V Zelepukin, A V Kabashin, S M Deev, A D Kaprin, P V Shegay, S A Ivanov, I N Zavestovskaya, S N Koryakin
We studied the antitumor activity of the combined use of local proton irradiation in two modes (10 and 31 Gy) with preliminary intra-tumoral injection of two types of bismuth nanoparticles differing in surface coating: coated with the amphiphilic molecule Pluronic-F127 or Silane-PEG (5 kDa)-COOH polymer. Nanoparticles were used in doses of 0.75 and 1.5 mg/mouse. In two independent series on experimental tumor model (solid Ehrlich carcinoma), bismuth nanoparticles of both modifications injected directly into the tumor enhanced the antitumor effects of proton therapy...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38730108/heat-shock-protein-hsp27-and-the-status-of-the-glutathione-system-in-dexamethasone-induced-apoptosis-of-jurkat-tumor-cells
#9
JOURNAL ARTICLE
O L Nosareva, E A Stepovaya, L S Litvinova, K A Yurova
We studied the effect of the HSP27 inhibitor, 5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)-isoxazole, at a final concentration of 0.1 μM and/or the apoptosis inducer dexamethasone at a final concentration of 10 μM on the content of hydroxyl radical, reduced and oxidized glutathione, HSP27, activity of glutathione reductase, glutathione peroxidase, caspase-3, and the number of Annexin+ Jurkat tumor cells. The involvement of HSP27 in apoptosis of Jurkat tumor cells was demonstrated. Simultaneous exposure to the HSP27 inhibitor and dexamethasone resulted in an increase in the level of HSP27 against the background of developing oxidative stress (increase in the concentration of hydroxyl radicals and changes in the state of the glutathione system)...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38730107/polymorphism-of-transforming-growth-factor-tgfb-and-its-receptors-tgfbri-tgfbrii-tgfbriiii-genes-in-western-siberia-patients-with-open-angle-glaucoma
#10
JOURNAL ARTICLE
A V Shevchenko, V F Prokofiev, V I Konenkov, V V Chernykh, A N Trunov
Polymorphism of genes of transforming growth factor TGFB and its receptors (TGFBRI, TGFBRII, and TGFBRIIII) in patients with primary open-angle glaucoma was analyzed. The frequency of the TGFBRII CC genotype in patients is increased relative to the control group (OR=6.10, p=0.0028). Heterozygosity in this polymorphic position is reduced (OR=0.18, p=0.0052). As the effects of TGF-β is mediated through its receptors, we analyzed complex of polymorphic variants of the studied loci in the genome of patients...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38730106/correlations-of-expression-levels-of-lung-cancer-marker-gene-eno2-and-genes-of-carcinogenesis-and-apoptosis-in-the-hypothalamus-of-mice-with-depression-like-behavior
#11
JOURNAL ARTICLE
A G Galyamina, I L Kovalenko, D A Smagin, N N Kudryavtseva
We experimentally demonstrated that chronic social stress during the development of a depression-like state enhances lung metastasis and modifies the expression of many carcinogenesis- and apoptosis-related genes in the hypothalamus of mice, including genes involved in lung cancer pathogenesis in humans. Analysis of the expression of genes encoding the major clinical markers of lung cancer in the hypothalamus of mice with depression-like behavior revealed increased expression of the Eno2 gene encoding neuron-specific enolase, a blood marker of lung cancer progression in humans...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38730105/killer-function-of-circulating-neutrophils-in-relation-to-cytokines-in-uterine-myoma-and-endometrial-cancer
#12
JOURNAL ARTICLE
T V Abakumova, I I Antoneeva, T P Gening
The study presents the killer functions of circulating neutrophils: myeloperoxidase activity, the ability to generate ROS, phagocytic activity, receptor status, NETosis, as well as the level of cytokines IL-2, IL-4, IL-6, IL-17A, and IL-18, granulocyte CSF, monocyte chemotactic protein 1, and neutrophil elastase in the serum of patients with uterine myoma and endometrial cancer (FIGO stages I-III). The phagocytic ability of neutrophils in uterine myoma was influenced by serum levels of granulocyte CSF and IL-2 in 54% of the total variance...
May 11, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38730104/radioprotective-properties-of-riboxin-inosine-and-indralin-under-external-irradiation
#13
JOURNAL ARTICLE
L A Romodin, O V Nikitenko, T M Bychkova, Yu A Zrilova, E D Rodionova, D A Bocharov
A comparative assessment of radioprotective properties of inosine nucleoside (riboxin) and recognized radioprotector indralin was carried out. We analyzed survival of male ICR CD-1 mice weighting 32.2±0.2 g exposed to external X-ray radiation at doses 6.5 and 6.75 Gy and receiving indralin at a dose of 100 or 150 μg/g body weight or riboxin (inosine) at a dose of 100 or 200 μg/g body weight before irradiation. The survival analysis was carried out by the Kaplan-Meier method. The significance was assessed by using the log-rank-test...
May 10, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38727956/features-of-remyelination-after-transplantation-of-olfactory-ensheathing-cells-with-neurotrophic-factors-into-spinal-cord-cysts
#14
JOURNAL ARTICLE
O V Stepanova, G A Fursa, E K Karsuntseva, S S Andretsova, A V Chadin, A D Voronova, V S Shishkina, A S Semkina, I V Reshetov, V P Chekhonin
This paper shows for the first time that co-transplantation of human olfactory ensheathing cells with neurotrophin-3 into spinal cord cysts is more effective for activation of remyelination than transplantation of cells with brain-derived neurotrophic factor and a combination of these two factors. The studied neurotrophic factors do not affect proliferation and migration of ensheathing cells in vitro. It can be concluded that the maximum improvement of motor function in rats receiving ensheathing cells with neurotrophin-3 is largely determined by activation of remyelination...
May 10, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38727955/changes-in-the-transcription-of-proliferation-and-apoptosis-related-genes-in-embryos-in-women-of-different-ages-under-the-influence-of-extracellular-vesicles-from-donor-follicular-fluid-in-vitro
#15
JOURNAL ARTICLE
O S Nepsha, O V Burmenskaya, Z F Akhmedova, E A Romanov, A P Sysoeva, K V Goryunov, Yu A Shevtsova, D N Silachev, N P Makarova, E A Kalinina
We studied the influence of extracellular vesicles from the follicular fluid of a young donor on gene expression (MKI67, MYBL2, CCNB1, CCND1, CCNE1, CALM2, BAX, NDRG1, TP53I3, VEGF, VCAN, HAS2, CTSL2, PIBF1, RPL37, PFKP, GPX3, and AQP3) in embryos of women of different ages. According to nanoparticle tracking analysis data, the concentration of extracellular vesicles was 3.75±0.47×1011 particles/ml and the mean particle size was 138.78±9.90 nm. During co-culturing of the follicular fluid extracellular vesicles with blastocysts of young women, we observed significantly increased expression of mRNA for genes CTSL2, CCND1, CCNE1, VEGF and reduced expression of BAX gene mRNA in comparison with embryos in women of late reproductive age...
May 10, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38727954/mutagenic-effect-during-combined-exposure-to-ionizing-and-non-ionizing-electromagnetic-radiation
#16
JOURNAL ARTICLE
E V Styazhkina, Yu R Akhmadullina, Yu V Gainetdinova, E A Payalova, E A Pryakhin
Using the method of dominant lethal mutations, we assessed the frequency of the death of Drosophila melanogaster embryos under combined exposure to ionizing γ-radiation and non-ionizing pulsed magnetic field at various doses and modes of exposure. Mutagenic effect of combined exposure is antagonistic in nature. The antagonism is more pronounced when the following mode of exposure was used: exposure to non-ionizing pulsed magnetic field for 5 h followed by exposure to γ-radiation at doses of 3, 10, and 60 Gy...
May 10, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38727953/hyaluronic-acid-as-a-lyve-1-receptor-ligand-in-the-lymphatic-system-of-healthy-human-skin
#17
JOURNAL ARTICLE
S V Michurina, N N Svechnikova, V I Konenkov, I Yu Ishchenko, S A Arkhipov, V V Arkhipova
Immunohistochemical detection of the LYVE-1 marker in healthy human full-thickness skin (the epidermis and the dermis) was carried out. LYVE-1 expression was found in the endothelium of lymphatic capillaries located in the papillary dermis, in the endothelium of larger lymphatic vessels of the reticular dermis, and in fibroblasts, which indicates their joint participation in hyaluronan metabolism. LYVE-1+ staining detected for the first time in cells of the stratum basale, the stratum spinosum, and the stratum granulosum of healthy human epidermis indicates their participation in hyaluronan metabolism and allows us to consider the spaces between epidermis cells as prelimphatics...
May 10, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38724817/analgesic-and-psychotropic-effects-of-a-new-bradykinin-antagonist
#18
JOURNAL ARTICLE
A E Aliforenko, V S Motov, V V Bykov, A V Bykova, V I Pavlovsky, V V Larchenko, V A Khazanov, A I Vengerovskii
A bradykinin B1 receptors antagonist PAV-0056, an 1,4-benzodiazepin-2-one derivative, intragastrically administrated to mice at doses of 0.1 and 1 mg/kg causes analgesia in the "formalin test" not inferior to that of diclofenac sodium (10 mg/kg) and tramadol (20 mg/kg). PAV-0056 at doses of 0.1 and 10 mg/kg has no anxiolytic and central muscle relaxant effects in mice and does not damage the gastric mucosa in rats. Based on the results of the conditioned place preference test, PAV-0056 also does not induce addiction in mice...
May 9, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38724815/modulators-of-the-nrf2-signaling-pathway-enhance-the-cytotoxic-effect-of-standard-chemotherapeutic-drugs-on-organoids-of-metastatic-colorectal-cancer
#19
JOURNAL ARTICLE
A V Razumovskaya, M O Silkina, S V Nikulin, A G Tonevitsky, B Ya Alekseev
The activity of known modulators of the Nrf2 signaling pathway (bardoxolone and brusatol) was studied on cultures of tumor organoids of metastatic colorectal cancer previously obtained from three patients. The effect of modulators was studied both as monotherapy and in combination with standard chemotherapy drugs used to treat colorectal cancer. The Nrf2 inhibitor brusatol and the Nrf2 activator bardoxolone have antitumor activity. Moreover, bardoxolone and brusatol also significantly enhance the effect of the chemotherapy drugs 5-fluorouracil, oxaliplatin, and irinotecan metabolite SN-38...
May 9, 2024: Bulletin of Experimental Biology and Medicine
https://read.qxmd.com/read/38724814/the-effect-of-therapy-regimen-on-antitumor-efficacy-of-the-nanosomal-doxorubicin-against-rat-glioblastoma-101-8
#20
JOURNAL ARTICLE
A I Alekseeva, A S Khalansky, E A Miroshnichenko, A D Gerasimov, A V Sentyabreva, V V Kudelkina, N S Osipova, M V Gulyaev, S E Gelperina, A M Kosyreva
One of the key problems of glioblastoma treatment is the low effectiveness of chemotherapeutic drugs. Incorporation of doxorubicin into PLGA nanoparticles allows increasing the antitumor effect of the cytostatics against experimental rat glioblastoma 101.8. Animal survival, tumor volume, and oncogene expression in tumor cells were compared after early (days 2, 5, and 8 after tumor implantation) and late (days 8, 11, and 14) start of the therapy. At late start, a significant increase in the expression of oncogenes Gdnf, Pdgfra, and Melk and genes determining the development of multidrug resistance Abcb1b and Mgmt was revealed...
May 9, 2024: Bulletin of Experimental Biology and Medicine
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