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Biochemical and Biophysical Research Communications

Kun-Feng Xie, Dong-Dong Guo, Xing-Jing Luo
Mitochondrial Ca2+ uptake, an important governing for Ca2+ homeostasis, is catalyzed by the mitochondrial calcium uniporter (MCU) complex. SMDT1, as a subunit of MCU complex, was essential for bridging the calcium-sensing role of MICU1 and MICU2 with the calcium-conducting role of MCU. However, the molecular mechanism and regulatory purpose of SMDT1 remain largely unexplored, especially no study was reported in cancer. Here, we firstly reported that how SMDT1 exerted its role through mediating mitochondrial dynamic in PDAC malignancy...
February 16, 2019: Biochemical and Biophysical Research Communications
Yuta Yoshida, Zhonghou Wang, Kayvan F Tehrani, Emily G Pendleton, Ryota Tanaka, Luke J Mortensen, Shotaro Nishimura, Shoji Tabata, Hong-Xiang Liu, Fuminori Kawabata
In the mammalian taste system, the taste receptor type 2 (T2R) family mediates bitter taste, and the taste receptor type 1 (T1R) family mediates sweet and umami tastes (the heterodimer of T1R2/T1R3 forms the sweet taste receptor, and the heterodimer of T1R1/T1R3 forms the umami taste receptor). In the chicken genome, bitter (T2R1, T2R2, and T2R7) and umami (T1R1 and T1R3) taste receptor genes have been found. However, the localization of these taste receptors in the taste buds of chickens has not been elucidated...
February 16, 2019: Biochemical and Biophysical Research Communications
Shimin Sun, Zuojun Liu, Yukun Feng, Lei Shi, Xinyue Cao, Yanlin Cai, Baohua Liu
Circadian clock relies on a transcription and translation feedback loop (TTFL). Two transcription factors, i.e. Bmal1 and Clock, activate the transcription of Period (Per) and Cryptochrome (Cry), which inhibit their own transcription when accumulated to a critical concentration. NAD+ -dependent deacylase Sirt1 deacetylates Bmal1 and Per2 to regulate circadian rhythms. Sirt6 interacts with Bmal1 to regulate clock-controlled gene (CCG) expression by local chromatin remodeling. Whether Sirt6 directly modify clock components is elusive...
February 16, 2019: Biochemical and Biophysical Research Communications
Markus Wortmann, Ekaterina Skorubskaya, Andreas S Peters, Maani Hakimi, Dittmar Böckler, Susanne Dihlmann
Abdominal aortic aneurysm (AAA) is a multi-factorial progressive vascular disease with life-threatening complications. Increasing evidence suggests that smooth muscle cell (SMC) dysfunction and cell death contribute to dilatation and rupture of the aorta by inducing an inflammatory response. The exact mechanism of this response however, is incompletely understood. We here investigated in vitro the capacity of autologous necrotic cell debris (CD) to induce inflammasome components and inflammatory mediators in aortic SMC (AAA-SMC) isolated from patients with AAA undergoing surgical repair...
February 16, 2019: Biochemical and Biophysical Research Communications
Qin Wang, Derun Wang, Shigeru Shibata, Tianrong Ji, Lei Zhang, Rui Zhang, He Yang, Linlin Ma, Jundong Jiao
Researches have shown that mice lacking the metabotropic glutamate receptor 1 (mGluR) showed albuminuria, remodeling of F-actin, with loss of stress fibers. Selective group I mGluRs agonist (S)-3,5-dihydroxyphenylglycine (DHPG) attenuated albuminuria in several rodent models of nephrotic syndrome. However, the molecular mechanism is obscure. Using a human podocyte cell line, we here investigated the molecular mechanisms of group I mGluRs-induced calcium influx and the formation of stress fibers. Our data showed that group I mGluRs activation by DHPG induced a significant calcium influx, and promoted cytoskeletal stress fiber formation and focal adhesions in podocytes...
February 16, 2019: Biochemical and Biophysical Research Communications
Caiyun Lai, Jianji Gao, Zhiliang Zhu, Jianhui Yuan, Wenjuan Zhang, Jianping Yang
Trichloroacetic acid (TCA) is one of the major metabolites of trichloroethylene (TCE) as the significant factor of environmental and occupational pollution. TCA has been shown to induce a series of epigenetic mutation in mouse liver. However, the epigenetic cytotoxicity of TCA is still in infancy. In this study, we explored the cellular biological characteristics, the genome DNA methylation status and the expression profile of DNA methyltransferases in human hepatic L-02 cells treated with TCA with certain time and dose effects...
February 16, 2019: Biochemical and Biophysical Research Communications
Hao Liang, Xiangqian Xiao, Xiaofei Zhang, Qin Hu, Yishu Yang, Shan Cen, Xiongwei Deng, Wang Sheng
Inactivated vaccines are widely used for prevention of viral disease. Both humoral and cellular immune responses have been shown to play important roles in the control and clearance of virus infections. CpG motif containing oligodeoxynucleotides (ODN) have recently gained considerable interest and been used as vaccine adjuvant due to their potent abilities to modulate host immune response. In this study, CpG-ODN adjuvant and inactivated viral particles of enterovirus 71 (EV71) were co-encapsulated into nanoparticles (NP) generated by using protamine sulfate (PS) and carboxymethyl β-glucan (CMG) through a self-assembly approach...
February 16, 2019: Biochemical and Biophysical Research Communications
Shaohui Zheng, Nana Yu, Cuiping Han, Ting Xie, Binru Dou, Ying Kong, Fengmei Zuo, Meilin Shi, Kai Xu
Gadolinium doped carbon dots (Gd-CDs) were prepared as a dual-modal imaging agent for enhanced MR imaging and cell fluorescence imaging. The Gd-CDs were synthesized via one-step solvent free technique with Gd-DTPA and l-arginine as the Gd and carbon sources with a quantum yield of 57.78%. The Gd-CDs exhibited good crystal structure, excellent aqueous dispersity, high colloidal stability, intense fluorescence and low cytotoxicity. The bio-TEM images revealed that the Gd-CDs could be easily internalized by cancer cells and escape from the endosomes...
February 15, 2019: Biochemical and Biophysical Research Communications
Eiji Kawamoto, Nodoka Nago, Takayuki Okamoto, Arong Gaowa, Asami Masui-Ito, Yosuke Sakakura, Yuichi Akama, Zay Yar Soe, Onmanee Prajuabjinda, Samuel Darkwah, Michael G Appiah, Phyoe Kyawe Myint, Gideon Obeng, Eun Jeong Park, Hiroshi Imai, Motomu Shimaoka
We reported previously that leukocyte β2 integrins (LFA-1 and Mac-1) bind to the serine/threonine-rich domain of thrombomodulin (TM) expressed on vascular endothelial cells (VECs). Recombinant human soluble TM (rhsTM, TMD123) has been approved as a therapeutic drug for septic disseminated intravascular coagulation. However, the roles of TMD123 on the adhesion of leukocyte integrins to VECs remain unclear. In the current study, we have revealed that an integrin-dependent binding between human peripheral blood mononuclear cells (PBMCs) and VECs was inhibited by TMD123...
February 15, 2019: Biochemical and Biophysical Research Communications
Sanghee Kang, Eui Hyun Kim, Jun-Eul Hwang, Ji-Hyun Shin, Yun Seong Jeong, Sun Young Yim, Eun Wook Joo, Young Gyu Eun, Dong Jin Lee, Bo Hwa Sohn, Sung Hwan Lee, Bora Lim, Ju-Seog Lee
High metabolic activity, reflected in increased glucose uptake, is one of the hallmarks of many cancers including breast cancer. However, not all cancers avidly take up glucose, suggesting heterogeneity in their metabolic demand. Thus, we aim to generate a genomic signature of glucose hypermetabolism in breast cancer and examine its clinical relevance. To identify genes significantly associated with glucose uptake, gene expression data were analyzed together with the standardized uptake values (SUVmax) of 18 F-fluorodeoxy-glucose on positron emission tomography (PET) for 11 breast cancers...
February 15, 2019: Biochemical and Biophysical Research Communications
Teppei Kitano, Soichiro Usui, Shin-Ichiro Takashima, Oto Inoue, Chiaki Goten, Ayano Nomura, Kazuaki Yoshioka, Masaki Okajima, Shuichi Kaneko, Yoh Takuwa, Masayuki Takamura
Vascular remodeling, resulting from proliferation and migration of vascular smooth muscle cells (VSMCs), is a major cause of atherosclerosis and restenosis. The lysophospholipid mediator sphingosine-1-phosphate (S1P) regulates proliferation and migration of VSMCs via S1P-specific G protein-coupled receptors, including S1P receptor 1 (S1PR1) to S1PR3. However, the role of S1PR1 in vascular remodeling is not well understood. Therefore, in this study, we aimed to investigate the effect of S1PR1 on neointimal hyperplasia in a carotid artery ligation mouse model using transgenic C57Bl/6 mice that overexpressed S1PR1 (Tg-S1PR1) under the control of α-smooth muscle actin promoter...
February 15, 2019: Biochemical and Biophysical Research Communications
Tatsuru Togo
Disruption and repair of plasma membranes is normally observed in many animal tissues. Recent studies demonstrated that wounding of Madin-Darby canine kidney cells potentiates membrane repair in cells adjacent to wounded cells via paracrine purinergic signaling. The present study demonstrated that cyclic adenosine monophosphate signaling in a wounded cell was induced by autocrine purinergic signaling, and protein kinase A potentiates membrane resealing for repeated wounds in those cells. Furthermore, the present study revealed that an increase in the intracellular free Ca2+ concentration upon cell membrane disruption was not only due to Ca2+ influx through the wound site, but also because of autocrine purinergic signaling...
February 15, 2019: Biochemical and Biophysical Research Communications
Satya Prakash Singh, Sebastian Raja, S Mahalingam
Vpx, a virion-associated protein of Human Immunodeficiency Virus 2 (HIV-2) and Simian Immunodeficiency Virus (SIV) counteracts host restriction factor SAMDH1 for efficient viral DNA synthesis in the cytoplasm and mediates subsequent nuclear translocation of the viral genome. Vpx was found to be indispensable in the viral infection of terminally differentiated target cells and macaques infected with virions carrying truncated Vpx showed delayed pathogenesis, suggesting multiple roles of Vpx at different steps in the virus life cycle...
February 15, 2019: Biochemical and Biophysical Research Communications
Zhou Jiangqiao, Qiu Tao, Chen Zhongbao, Ma Xiaoxiong, Zhang Long, Zou Jilin, Wang Tianyu
Clear cell renal cell carcinoma (ccRCC) is one of the most common malignancies. Anti-silencing function 1B histone chaperone (ASF1B) has been reported to be involved in various diseases. However, its role in ccRCC is largely unknown. In the present study, using genetic data and clinical information obtained from the TCGA data portal and GEO database, we found that ASF1B was highly expressed in ccRCC cancer tissue compared with normal tissue, and ASF1B expression was positively correlated with tumor stage, tumor grade and patient survival...
February 15, 2019: Biochemical and Biophysical Research Communications
Saki Yoshimura, Shihoko Nakashima, Yuki Tomiga, Shotaro Kawakami, Yoshinari Uehara, Yasuki Higaki
No abstract text is available yet for this article.
February 15, 2019: Biochemical and Biophysical Research Communications
Hanna E Teasley, Munseok Paul Jeong, Tae Hoon Kim
The ovarian steroid hormones, estrogen (E2) and progesterone (P4), are essential regulators of uterine biology. The imbalance of these ovarian steroid hormones leads to uterine diseases such as endometrial cancer, endometriosis, and infertility. Mitogen-inducible gene 6 (MIG-6) is an adaptor protein. MIG-6 mediates P4 signaling and acts as a tumor suppressor during endometrial tumorigenesis in both humans and mice. In previous studies, we developed the conditional knockout of Mig-6 in all uterine compartments (Pgrcre/+ Mig-6f/f ; Mig-6KO ) and endometrial epithelial cell-specific Mig-6 knockout (Sprr2fcre/+ Mig-6f/f ; Mig-6Ep-KO ) mice...
February 14, 2019: Biochemical and Biophysical Research Communications
Yusuke Kishimoto, Shingo Kasamatsu, Shuichi Yanai, Shogo Endo, Takaaki Akaike, Hideshi Ihara
We previously reported that 8-nitroguanosine 3',5'-cyclic monophosphate (8-nitro-cGMP) is endogenously produced via nitric oxide/reactive oxygen species signaling pathways and it reacts with protein thiol residues to add cGMP structure to proteins through S-guanylation. S-Guanylation occurs on synaptosomal-associated protein 25 (SNAP-25), which is a part of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex that regulates exocytosis. However, the biological relevance of 8-nitro-cGMP in the nervous system remains unclear...
February 14, 2019: Biochemical and Biophysical Research Communications
Laura J A Hardwick, John D Davies, Anna Philpott
The superfamily of basic-Helix-Loop-Helix (bHLH) transcription factors influence cell fate in all three embryonic germ layers, and the tissue-specific class II factors have received prominent attention for their potent ability to direct differentiation during development and in cellular reprogramming. The activity of many class II bHLH proteins driving differentiation, and the inhibitory class VI bHLH factor Hes1, is controlled by phosphorylation on multiple sites by Cyclin-dependent kinases (Cdks). As class II proteins are generally thought to be active through hetero-dimerisation with the ubiquitously expressed class I E proteins, regulation of class I transcription factors such as E47 may influence the activity of multiple tissue-specific bHLH proteins...
February 14, 2019: Biochemical and Biophysical Research Communications
Yang Liu, Eleni Dakou, Ying Meng, Luc Leyns
Isolated mouse embryonic stem cells (mESCs) retain the capacities to self-renew limitlessly and to give rise to all tissues of an adult mouse. A precise understanding of the relationships, mechanisms of action and functions of novel genes involved in mESCs differentiation is crucial to expand our knowledge of vertebrate development. The epithelial membrane protein 2 (EMP2) is a membrane-spanning protein found in epithelial and endothelial cell-cell junctions that has been implicated in the regulation of cell proliferation and migration in normal and tumor tissues...
February 14, 2019: Biochemical and Biophysical Research Communications
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