journal
Journals Biochemical and Biophysical Re...

Biochemical and Biophysical Research Communications

https://read.qxmd.com/read/38901057/usp11-modulates-mitotic-progression-and-senescence-by-regulating-the-p53-p21-axis-through-mdm2-deubiquitination
#1
JOURNAL ARTICLE
Won-Joo Kim, Abdul Basit, Jae-Ho Lee
USP11 is overexpressed in colorectal cancer (CRC) and breast cancer tissues compared to normal tissues, suggesting a role in promoting cell proliferation and inhibiting cell death. In this study, we observed that depleting USP11 inhibits cell proliferation and delays cell cycle progression. This depletion leads to increased p53 protein levels due to an extended half-life, resulting in elevated p21 mRNA levels in a p53-dependent manner. The rise in p53 protein upon USP11 depletion is linked to a reduced half-life of MDM2, a known E3 ligase for p53, via enhanced polyubiquitination of MDM2...
June 18, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38901224/adolescent-administration-of-ketamine-impairs-excitatory-synapse-formation-onto-parvalbumin-positive-gabaergic-interneurons-in-mouse-prefrontal-cortex
#2
JOURNAL ARTICLE
Jia-Wei Zhang, Hai-Qian Zhou, Zhen Zhu, Yang-Yang Ding, Ying He, Xiao-Lian Wei, Chen-Fan Xiao, Yun-Fei Li, Wei-Peng Lin, Dong-Min Yin
Ketamine, an N-methyl-d-aspartate (NMDA) receptor antagonist, induces deficits in cognition and information processing following chronic abuse. Adolescent ketamine misuse represents a significant global public health issue; however, the neurodevelopmental mechanisms underlying this phenomenon remain largely elusive. This study investigated the long-term effects of sub-chronic ketamine (Ket) administration on the medial prefrontal cortex (mPFC) and associated behaviors. In this study, Ket administration during early adolescence displayed a reduced density of excitatory synapses on parvalbumin (PV) neurons persisting into adulthood...
June 15, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38901222/structural-insights-into-the-allosteric-effects-of-the-antiepileptic-drug-topiramate-on-the-ca-v-2-3-channel
#3
JOURNAL ARTICLE
Yiwei Gao, Qinru Bai, Xuejun Cai Zhang, Yan Zhao
The R-type voltage-gated calcium channel CaV 2.3 is predominantly located in the presynapse and is implicated in distinct types of epileptic seizures. It has consequently emerged as a molecular target in seizure treatment. Here, we determined the cryo-EM structure of the CaV 2.3-α2δ1-β1 complex in the topiramate-bound state at a 3.0 Å resolution. We provide a snapshot of the binding site of topiramate, a widely prescribed antiepileptic drug, on a voltage-gated ion channel. The binding site is located at an intracellular juxtamembrane hydrophilic cavity...
June 15, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38901225/palliative-effect-of-rotating-magnetic-field-on-glucocorticoid-induced-osteonecrosis-of-the-femoral-head-in-rats-by-regulating-osteoblast-differentiation
#4
JOURNAL ARTICLE
Hua Yang, Shenglan Nie, Cai Zhou, Mengqing Li, Qinyao Yu, Yaxian Mo, Yunpeng Wei, Xiaomei Wang
With the substantial increase in the overuse of glucocorticoids (GCs) in clinical medicine, the prevalence of glucocorticoid-induced osteonecrosis of the femoral head (GC-ONFH) continues to rise in recent years. However, the optimal treatment for GC-ONFH remains elusive. Rotating magnetic field (RMF), considered as a non-invasive, safe and effective approach, has been proved to have multiple beneficial biological effects including improving bone diseases. To verify the effects of RMF on GC-ONFH, a lipopolysaccharide (LPS) and methylprednisolone (MPS)-induced invivo rat model, and an MPS-induced invitro cell model have been employed...
June 14, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38880081/intermediate-open-state-of-cd4-bound-hiv-1-env-heterotrimers-in-asia-crfs
#5
JOURNAL ARTICLE
Dan Li, Li Liu, Xuejun Ye, Yuyang Chen, Qiaoju Ren, ShaoJian Xu, Yan Ren, He Cao, Tao Wang
The HIV-1 envelope glycoprotein (Env) plays crucial role in viral infection by facilitating viral attachment to host cells and inducing fusion of the virus with the host cell membrane. This fusion allows the HIV-1 viral genome to enter the target cell then triggering various stages of the viral life cycle. The native Env directly interacts with the main receptor CD4 and the co-receptor (CCR5 or CXCR4) in human cell membrane then induces membrane fusion. The elucidation of the structure of Env with CD4 and co-receptors in different HIV-1 subtypes is essential for the understanding of the mechanism of virus entry...
June 13, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38878759/diurnal-variation-of-cisplatin-induced-renal-toxicity-in-icr-mice
#6
JOURNAL ARTICLE
Sarah Tominaga, Hiroki Yoshioka, Tatsuya Hasegawa, Masumi Suzui, Maeda Tohru, Nobuhiko Miura
Cisplatin (CDDP) is a platinum-based anticancer drug widely prescribed for its effectiveness in treating various forms of cancer. However, its major side effect is nephrotoxicity. Although several methods have been developed to mitigate CDDP-induced nephrotoxicity, an optimal approach has yet to be established. This study aimed to investigate the "chronotoxicity" of CDDP as a potential strategy to reduce its side effects. Male ICR mice were treated with CDDP (20 mg/kg, intraperitoneal injection, one shot) at zeitgeber time (ZT) 2 or ZT14 (light or dark phase)...
June 13, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38897040/inhibition-of-soluble-epoxide-hydrolase-by-natural-isothiocyanates
#7
JOURNAL ARTICLE
Fawzy Elbarbry, Michael J Espiritu, Kaylen Soo, Baily Yee, Jonathan Taylor
GOAL: The long-term goal of our research is to develop safe and effective soluble epoxide hydrolase (sEH) inhibitors. The objective of this study is to evaluate the potency and selectivity of six natural isothiocyanates (ITCs) as sEH inhibitors. METHODS: Molecular docking was used to model likely interactions between the ligands and receptors. The sEH inhibitory activity was tested using a validated fluorescence-based assay and PHOME as a substrate. To evaluate their selectivity as sEH inhibitors, the inhibitory potential of the ITCs was determined on microsomal epoxide hydrolase (mEH) and cytochrome P450 (CYP) enzymes in human liver microsomes...
June 12, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38878760/enhanced-brain-targeting-and-efficacy-of-cannabidiol-via-rvg-exo-cbd-nanodelivery-system
#8
JOURNAL ARTICLE
Yingrui Li, Ze Chen, Jia Guo, Deshuang Meng, Xin Pang, Zepeng Sun, Li Pu, Shuiyue Yang, Min Yang, Yinghua Peng
This study introduces an innovative brain-targeted drug delivery system, RVG-Exo/CBD, utilizing rabies virus glycoprotein (RVG)-engineered exosomes for encapsulating cannabidiol (CBD). The novel delivery system was meticulously characterized, confirming the maintenance of exosomal integrity, size, and successful drug encapsulation with a high drug loading rate of 83.0 %. Evaluation of the RVG-Exo/CBD's brain-targeting capability demonstrated superior distribution and retention in brain tissue compared to unmodified exosomes, primarily validated through in vivo fluorescence imaging...
June 12, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38901226/metabolic-determinants-of-leukemia-onset-variability-in-genetically-homogeneous-akr-mice
#9
JOURNAL ARTICLE
Hoonsik Nam, Doyeon Kim, Xing Jin, Sunghyouk Park
Leukemia is a complex disease shaped by the intricate interplay of genetic and environmental factors. Given our preliminary data showing different leukemia incidence in genetically homogenous AKR mice harboring the spontaneous leukemia-inducing mutation Rmcfs, we sought to unravel the role of metabolites and gut microbiota in the leukemia penetrance. Our metabolomic analysis revealed distinct serum metabolite profiles between mice that developed leukemia and those that did not. We discovered that linoleic acid (LA), an essential ω-6 polyunsaturated fatty acid, was significantly decreased in the leukemia group, with the lower levels observed starting from 25 weeks before the onset...
June 11, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38901223/dapagliflozin-administration-to-a-mouse-model-of-type-2-diabetes-induces-dna-methylation-and-gene-expression-changes-in-pancreatic-islets
#10
JOURNAL ARTICLE
Aisha Yokoi, Shun-Ichiro Asahara, Hiroyuki Inoue, Masako Seike, Nozomi Kido, Hirotaka Suzuki, Ayumi Kanno, Maki Kimura-Koyanagi, Yoshiaki Kido, Wataru Ogawa
Decreased pancreatic β-cell volume is a serious problem in patients with type 2 diabetes mellitus, and there is a need to establish appropriate treatments. Increasingly, sodium/glucose cotransporter 2 (SGLT2) inhibitors, which have a protective effect on pancreatic β-cells, are being prescribed to treat diabetes; however, the underlying mechanism is not well understood. We previously administered SGLT2 inhibitor dapagliflozin to a mouse model of type 2 diabetes and found significant changes in gene expression in the early-treated group, which led us to hypothesize that epigenetic regulation was a possible mechanism of these changes...
June 11, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38897043/3d-layered-co-culture-model-enhances-trastuzumab-deruxtecan-sensitivity-and-reveals-the-combined-effect-with-g007-lk-in-her2-positive-non-small-cell-lung-cancer
#11
JOURNAL ARTICLE
Akari Shiraishi, Tomoko Oh-Hara, Yuki Takahashi, Ken Uchibori, Makoto Nishio, Ryohei Katayama
Human epidermal growth factor receptor 2 (HER2) aberrations are observed in various cancers. In non-small cell lung cancer, genetic alterations activating HER2, mostly exon 20 insertion mutations, occur in approximately 2-4% of cases. Trastuzumab deruxtecan (T-DXd), a HER2-targeted antibody-drug conjugate has been approved as the first HER2-targeted drug for HER2-mutant lung cancer. However, some cases are not responsive to T-DXd and the primary resistant mechanism remains unclear. In this study, we assessed sensitivity to T-DXd in JFCR-007, a patient-derived HER2-mutant lung cancer cell line...
June 11, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38897041/hepatoprotective-effects-of-zingerone-on-sodium-arsenite-induced-hepatotoxicity-in-rats-modulating-the-levels-of-caspase-3-bax-bcl-2-nlrp3-nf-%C3%AE%C2%BAb-tnf-%C3%AE-and-atf6-ire1-perk-grp78-signaling-pathways
#12
JOURNAL ARTICLE
Berna Eriten, Cuneyt Caglayan, Cihan Gür, Sefa Küçükler, Halit Diril
OBJECTIVE: Long-term exposure to arsenic has been linked to several illnesses, including hypertension, diabetes, hepatic and renal diseases and cardiovascular malfunction. The aim of the current investigation was to determine whether zingerone (ZN) could shield rats against the hepatotoxicity that sodium arsenite (SA) causes. METHODS: The following five groups of thirty-five male Sprague Dawley rats were created: I) Control; received normal saline, II) ZN; received ZN, III) SA; received SA, IV) SA + ZN 25; received 10 mg/kg body weight SA + 25 mg/kg body weight ZN, and V) SA + ZN 50; received 10 mg/kg body weight SA + 50 mg/kg body weight ZN...
June 11, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38880080/deciphering-the-drug-delivery-potential-of-type1-lipid-transfer-protein-from-citrus-sinensis-for-enhancing-the-therapeutic-efficacy-of-drugs
#13
JOURNAL ARTICLE
Surabhi Rode, Harry Kaur, Ruchi Rani, Sapna Lonare, Monica Sharma, Shailly Tomar, Pravindra Kumar, Partha Roy, Ashwani Kumar Sharma
Type1 Non-specific Lipid Transfer Protein (CsLTP1) from Citrus sinensis is a small cationic protein possessing a long tunnel-like hydrophobic cavity. CsLTP1 performing membrane trafficking of lipids is a promising candidate for developing a potent drug delivery system. The present work includes in-silico studies and the evaluation of drugs binding to CsLTP1 using biophysical techniques along with the investigation of CsLTP1's ability to enhance the efficacy of drugs employing cell-based bioassays. The in-silico investigations identified Panobinostat, Vorinostat, Cetylpyridinium Chloride, and Fulvestrant with higher affinities and stability of binding to the hydrophobic pocket of CsLTP1...
June 11, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38878758/determinants-of-the-interaction-between-the-5-leader-of-hiv-1-genome-and-human-lysyl-trna-synthetase-in-reverse-transcription-primer-release-process
#14
JOURNAL ARTICLE
Yong Liu, Zhi Luo, Xiang Chen, Xin Yang, Qi Qi, Mailikezhati Alifu, Chengcheng Tao, Wen Cui, Mengmeng Liu, Wei Wang
Reverse transcription of human immunodeficiency virus type 1 (HIV-1) initiates from the 3' end of human tRNALys3 . The primer tRNALys3 is selectively packaged into the virus in the form of a complex with human lysyl-tRNA synthetase (LysRS). To facilitate reverse transcription initiation, part of the 5' leader (5'L) of HIV-1 genomic RNA (gRNA) evolves a tRNA anticodon-like element (TLE), which binds LysRS and releases tRNALys3 for primer annealing and reverse transcription initiation. Although TLE has been identified as a key element in 5'L responsible for LysRS binding, how the conformations and various hairpin structures of 5'L regulate 5'L-LysRS interaction is not fully understood...
June 10, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38870847/inhibition-of-cirbp-represses-the-proliferation-and-migration-of-vascular-smooth-muscle-cells-via-inhibiting-rheb-mtorc1-axis
#15
JOURNAL ARTICLE
Jiaqi Zhao, Chenming Qiu, Rong Wan, Qiang Wang, Yan Zhang, Dachun Yang, Yongjian Yang, Xiongshan Sun
The excessive migration and proliferation of vascular smooth muscle cells (VSMCs) plays a vital role in vascular intimal hyperplasia. CIRBP is involved in the proliferation of various cancer cells. This study was aimed to explore the role of CIRBP in the proliferation and migration of VSMCs. Adenovirus was used to interfere with cold-inducible RNA-binding protein (CIRBP) expression, while lentivirus was used to overexpress Ras homolog enriched in brain (Rheb). Western blotting and qRT-PCR were used to evaluate the expression of CIRBP, Rheb, and mechanistic target of rapamycin complex 1 (mTORC1) activity...
June 10, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38870846/dissecting-the-roles-of-dynamin-and-clathrin-in-platelet-pinocytosis
#16
JOURNAL ARTICLE
Ruby M Baxter, Matthew T Harper
Platelets endocytose many molecules from their environment. However, this process of pinocytosis in platelets is poorly understood. Key endocytic regulators such as dynamin, clathrin, CDC42 and Arf6 are expressed in platelets but their roles in pinocytosis is not known. Stimulated platelets form two subpopulations of pro-aggregatory and procoagulant platelets. The effect of stimulation on pinocytosis is also poorly understood. In this study, washed human platelets were treated with a range of endocytosis inhibitors and stimulated using different activators...
June 10, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38897042/structure-of-gdp-bound-rab7-q67l-in-complex-with-orp1l
#17
JOURNAL ARTICLE
Qian Lu, Zhongliang Zhu, Jiahai Zhang, Chao Xu
Molecular processes are orchestrated by various proteins that promote early endosomes to become late endosomes and eventually fuse with lysosomes, guaranteeing the degradation of the content. Rab7, which is localized to late endosomes, is one of the most well-known GTPases. ORP1L is recruited by Rab7 to facilitate the fusion of late endosomes and lysosomes. Here, we present the structure of GDP-bound Rab7 Q67L with ORP1L. Structural analysis, supported by biochemical and ITC binding experiments, not only provides structural insight into the interactions between the ORP1L ANK domain and Rab7 but also suggests that the GTPase activity of Rab7 does not interfere with its ORP1L-binding capacity...
June 9, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38865815/protective-effects-of-tauroursodeoxycholate-against-radiation-induced-intestinal-injury-in-a-mouse-model
#18
JOURNAL ARTICLE
Jeongmin Lee, Byung-Suk Jeon, Sohi Kang, Yeonghoon Son, Young-Bin Lim, Min Ji Bae, Wol Soon Jo, Chang-Geun Lee, In Shik Shin, Changjong Moon, Hae-June Lee, Joong-Sun Kim
In patients with high-level radiation exposure, gastrointestinal injury is the main cause of death. Despite the severity of damage to the gastrointestinal tract, no specific therapeutic option is available. Tauroursodeoxycholic acid (TUDCA) is a conjugated form of ursodeoxycholic acid that suppresses endoplasmic reticulum (ER) stress and regulates various cell-signaling pathways. We investigated the effect of TUDCA premedication in alleviating intestinal damage and enhancing the survival of C57BL/6 mice administered a lethal dose (15Gy) of focal abdominal irradiation...
June 7, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38865814/arabidopsis-cryptochromes-interact-with-sog1-to-promote-the-repair-of-dna-double-strand-breaks
#19
JOURNAL ARTICLE
Li Chen, Minqing Liu, Yupeng Li, Yan Guan, Jiaqi Ruan, Zhilei Mao, Wenxiu Wang, Hong-Quan Yang, Tongtong Guo
Cryptochromes (CRYs) are blue light (BL) photoreceptors to regulate a variety of physiological processes including DNA double-strand break (DSB) repair. SUPPRESSOR OF GAMMA RADIATION 1 (SOG1) acts as the central transcription factor of DNA damage response (DDR) to induce the transcription of downstream genes, including DSB repair-related genes BRCA1 and RAD51. Whether CRYs regulate DSB repair by directly modulating SOG1 is unknown. Here, we demonstrate that CRYs physically interact with SOG1. Disruption of CRYs and SOG1 leads to increased sensitivity to DSBs and reduced DSB repair-related genes' expression under BL...
June 7, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38897039/plexin-d1-negatively-regulates-macrophage-derived-foam-cell-migration-via-the-focal-adhesion-kinase-paxillin-pathway
#20
JOURNAL ARTICLE
Chenlei Li, Yan Niu, Jie Chen, Shijia Geng, Peng Wu, Lina Dai, Chongyang Dong, Rujin Liu, Yuanjia Shi, Xiaomeng Wang, Zhanfeng Gao, Xiaoyu Liu, Xi Yang, Shang Gao
BACKGROUND: Macrophage-derived foam cell formation is a hallmark of atherosclerosis and is retained during plaque formation. Strategies to inhibit the accumulation of these cells hold promise as viable options for treating atherosclerosis. Plexin D1 (PLXND1), a member of the Plexin family, has elevated expression in atherosclerotic plaques and correlates with cell migration; however, its role in macrophages remains unclear. We hypothesize that the guidance receptor PLXND1 negatively regulating macrophage mobility to promote the progression of atherosclerosis...
June 6, 2024: Biochemical and Biophysical Research Communications
journal
journal
23509
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.