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Experimental and Molecular Pathology

Yang Jing, Wang Zongming, Zhang Xilin
PURPOSE: Liraglutide, a glucagon-like peptide-1 (GLP-1) analogue, is an antidiabetic drug. It has been shown to improve the Psoriasis Area and Severity Index (PASI) in patients with type 2 diabetes and psoriasis in clinical practice, but the mechanism remains somewhat unclear. We used lipopolysaccharides (LPS) to induce inflammatory response in keratinocytes and explore the mechanism. METHODS: The HaCat cells were incubated with LPS for 16 h and then were treated with liraglutide for 30 min...
February 15, 2019: Experimental and Molecular Pathology
Vishal Sahu, Anant Mohan, Sharmistha Dey
INTRODUCTION: p38 MAPK signaling molecules plays a dual role in cancer, both progression and suppression. Elevated expression of p38α was reported in lung cancer tissue in rat model. Our objective was to explore the concentration of all 4 isoforms of p38MAPK in serum of Non Small Cell Lung Cancer (NSCLC). MATERIAL AND METHODS: The blood samples were collected from 77 NSCLC patients, 52 ethically matched healthy controls and 18 follow up patients were collected as some patients expired and some discontinued the treatment...
February 13, 2019: Experimental and Molecular Pathology
Lu Zhang, Bin Luo, Yi-Wu Dang, Rong-Quan He, Gang Chen, Zhi-Gang Peng, Zhen-Bo Feng
OBJECTIVE: To explore the clinical significance and potential molecular mechanism of endothelin receptor type B (EDNRB) in hepatocellular carcinoma (HCC). METHODS: Immunohistochemistry was used to detect EDNRB protein expression level in 67 HCC paraffin embedded tissues and adjacent tissues. Correlations between EDNRB expression level and clinicopathologic parameters were analyzed in our study. The expression level and clinical significance of EDNRB in HCC were also evaluated from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database...
February 12, 2019: Experimental and Molecular Pathology
He Hu, Chuanlong Zhao, Peiguang Zhang, Yalong Liu, Yulian Jiang, Enquan Wu, Hao Xue, Caiyun Liu, Zhehai Li
BACKGROUNDS: Osteoactivin (OA) is a key regulator promoting bone marrow stromal cells osteogenesis progress, while Dexamethasone (Dex) could inhibit OA induced osteogenesis and lead to osteoporosis. miR-26b increased during BMSC osteogenesis but whether it participates in this progress is enigma. Osteogenesis is under regulation of canonical Wnt signaling pathway which could serve as potential target for miR-26b. It bears therapeutic potential if miR-26b could regulate osteogenesis and antagonize Dex induced Osteoporosis (OP)...
February 12, 2019: Experimental and Molecular Pathology
Anna Kurpińska, Joanna Suraj, Emilia Bonar, Agnieszka Zakrzewska, Marta Stojak, Magdalena Sternak, Agnieszka Jasztal, Maria Walczak
INTRODUCTION: The tumor-promoting rearrangement of the lungs facilitates the process of cancer cell survival in a foreign microenvironment and enables their protection against immune defense. The study aimed to define the fingerprint of the early rearrangement of the lungs via the proteomic profiling of the lung tissue in the experimental model of tumor metastasis in a murine 4 T1 mammary adenocarcinoma. MATERIALS AND METHODS: The studies were performed on 7-8-week-old BALB/c female mice...
February 11, 2019: Experimental and Molecular Pathology
Alexander Tarek El Gammal, Nathaniel Melling, Matthias Reeh, Florian Gebauer, Oliver Mann, Daniel Perez, Maximillian Bockhorn, Kai Bachmann, Jakob Robert Izbicki, Katharina Grupp
Expression of the retinoic acid-induced protein 3 (RAI3) has been suggested to predict clinical outcome in a variety of malignancies. However, its role in esophageal cancers remains unclear. Immunohistochemical RAI3 staining was analyzed on tissue microarrays containing 359 esophageal adenocarcinomas (EAC) and 254 esophageal squamous cell carcinomas (ESCC). RAI3 immunostaining was typically absent or weakly detectable in the membranes in benign esophageal tissues. RAI3 staining was higher in malignant than in benign esophagus epithelium...
January 29, 2019: Experimental and Molecular Pathology
Cheng-Gong Wang, Zhan Liao, Han Xiao, Hua Liu, Yi-He Hu, Qian-De Liao, Da Zhong
BACKGROUND: Osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is of much significance for bone formation, the imbalance of it would result in osteoporosis and other pathological bone defects. Increasing evidences showed that long non-coding RNAs (lncRNAs) and miRNAs played vital roles in the regulation of osteogenic differentiation. LncRNA KCNQ1OT1 was often regarded as an imprinted lncRNA and was related to tumor progression, while its function in osteogenic differentiation remained unclear...
January 28, 2019: Experimental and Molecular Pathology
Fumei Chen, Mengyuan Li, Xiaoping Zhu
BACKGROUND: Propofol is a popular anesthetic agent, with potent anti-tumor activity against many cancers. The objective of this study was to explore the potential effect of propofol on papillary thyroid cancer (PTC) in vitro. METHODS: Human PTC cell lines TPC-1 and IHH-4 were treated by propofol. ANRIL expression vector (pc-ANRIL) was transfected into TPC-1 cells to overexpress the expression of ANRIL. CCK-8, BrdU assay, transwell assay, flow cytometry and Western blot were performed to evaluate cell proliferation, migration and apoptosis...
January 28, 2019: Experimental and Molecular Pathology
Lijuan Zhang, Li Wang, Enyu Guo, Yuefeng Qi
BACKGROUND: Cardiac hypertrophy related factor (CHRF), a newly recognized long non-coding RNA (lncRNA), is a central regulator in cardiac hypertrophy responses. This study attempted to show the potential role of lncRNA CHRF in bacterial infection caused myocarditis. METHODS: H9c2 cells were transfected with small interfering RNAs (siRNAs) specific for lncRNA CHRF alone or in combination with miR-221 inhibitor, and then subjected to lipopolysaccharide (LPS). The following parameters were measured: cell viability, apoptosis, reactive oxygen species (ROS) generation, pro-inflammatory cytokines release, microRNA (miR)-221 expression and the activation of nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) pathways...
January 26, 2019: Experimental and Molecular Pathology
Yuxi Qi, Jinliang Li
BACKGROUND: Triptolide (TPL) is the active component of Tripterygium wifordii Hook F, which has been reported to exert anti-tumor efficacies. Herein, we aimed to examine the efficacy of TPL in colorectal cancer (CRC) cells and to reveal its underlying mechanisms. METHODS: Human CRC cell lines HT-29 and SW480 were treated by TPL for 24 h in the presence or absence of epithelial-to-mesenchymal transition (EMT) inducer TGF-β1. The expression of miR-191 in cell was overexpressed by miRNA transfection...
January 23, 2019: Experimental and Molecular Pathology
Andrés J Medina, Oscar A Pinilla, Enrique L Portiansky, Claudia I Caldiz, Irene L Ennis
Chronic activation of the renin angiotensin system (RAS) favors several cardiac diseases, among which myocardial hypertrophy occupies an outstanding place. In this context, the hyperactivity of the cardiac Na+ /H+ (NHE-1) exchanger plays a key role. The pathologic remodeling of the myocardium constitutes an independent risk factor for morbidity and mortality with continuously increasing healthcare cost. Therefore, the development of better therapeutic strategies emerges as highly mandatory. Our goal was to prevent angiotensin II (ANGII)-induced cardiac hypertrophy by NHE-1 gene silencing in Wistar rats...
January 18, 2019: Experimental and Molecular Pathology
Anna L Greenshields, Wasundara Fernando, David W Hoskin
Breast cancer is the most prevalent cancer diagnosis in women, with triple-negative and human epidermal growth factor 2 (HER2)-enriched advanced breast cancers having the poorest prognoses. The morbidity and mortality associated with advanced disease, as well as the emergence of multi-drug resistant variants, highlights the urgency to develop novel therapeutic agents. Artesunate (ART) is a semi-synthetic derivative of artemisinin from the Chinese herb sweet wormwood. ART is widely used in the treatment of malaria and is well tolerated by patients...
January 17, 2019: Experimental and Molecular Pathology
Liana S Gomes, Janaína R Costa, Mônica S Campos, Mara R Marques, Manoel F Biancardi, Sebastião R Taboga, Paulo C Ghedini, Fernanda C A Santos
Normal prostate development is highly dependent of an equilibrated hormonal regulation, so that sensible interferences during this period may predispose the gland to lesions during aging. Industrial activities have increased the exposure of this gland to active elements found in environment, such as aluminum (Al). Al presents toxic effect for living beings, having the potential to disrupt the development and growth of several organs and systems. Therefore, the aim of this study was to evaluate whether the prenatal exposure to Al may alter the development and morphophysiology of the gerbil prostate (Meriones unguiculatus)...
January 16, 2019: Experimental and Molecular Pathology
Jun Wang, Depei Wu, Aining Sun
BACKGROUND: Whether glutathione S-transferases (GST) null genotypes influence individual susceptibility to leukemia remains controversial. Thus, we performed this meta-analysis to better analyze potential influences of GST null genotypes on individual susceptibility to leukemia. METHODS: Literature retrieve was conducted in PubMed, Web of Science and Embase. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. RESULTS: Totally fifty-one studies were enrolled for analyses...
January 16, 2019: Experimental and Molecular Pathology
Jinliang Li, Yuxi Qi
BACKGROUND: Colorectal cancer (CRC) is a troublesome disease with high morbidity and mortality. Ginsenoside Rg3 possesses anti-cancer properties. Colon Cancer Associated Transcript 1 (CCAT1) participates in the genesis, development, invasion and metastasis of colorectal cancer. In our study, we explored the effects of Rg3 on CRC cell line Caco-2 by regulating CCAT1. METHODS: CRC tissue was obtained from hospital and Caco-2 cells were purchased. Caco-2 cells were treated with Rg3 and/or transfected with pc- CCAT1 or pcDNA3...
January 8, 2019: Experimental and Molecular Pathology
Chunhua Dai, Shanhao Jiang, Cuiying Chu, Meng Xin, Xiufen Song, Bojun Zhao
BACKGROUND: Diabetic retinopathy (DR) is a common complication of diabetes mellitus, which is a major reason of blindness. Baicalin (BAI) is a flavonoid extracted from Scutellaria baicalensis, whose pharmacological characterizes have been widely reported in various diseases. However, it remains unclear the effect of BAI on DR. The study aimed to confirm the protective effect of BAI on DR. METHODS: ARPE-19 cells and HRMECs were exposed to the high glucose (HG) environment to construct a cell injury model...
January 6, 2019: Experimental and Molecular Pathology
Lin Long, Hua Xiang, Jueshi Liu, Zhiming Zhang, Lin Sun
The acquired chemoresistance during long term chemotherapy is one of the most important factors to limit the application of Doxorubicin (Dox) on clinical treatment of hepatocellular carcinoma (HCC) patients. Our present study found that Dox resistant HCC (HCC/Dox) cells had greater capability of in vitro migration and invasion compared to their parental cells. HCC/Dox cells exhibited mesenchymal characteristics, which was evidenced by the up regulation of fibronectin, vimentin while down regulation of E-Cadherin...
January 4, 2019: Experimental and Molecular Pathology
Zhongyan Mou, Yan Wang, Yongmei Li
BACKGROUND: Acoustic neuroma is a benign and usually slow growing tumor. Brazilein is a natural compound extracted from hematoxylin. However, there has been no study of the mechanism of brazilein in acoustic neuroma cells. Thus, we aimed to investigate the effects and mechanism of brazilein on human VS cells in this study. METHODS: The vestibular schwannoma (VS) cells were collected from patient tissues and used in this study. Different concentrations of brazilein (0, 10, 20 and 30 μM) were used to treat VS cells...
January 2, 2019: Experimental and Molecular Pathology
Hye-Young Wang, Hyunil Kim, Hyeonseok Yoon, Kwang Hwa Park, Hyeyoung Lee
Recent studies have demonstrated low specificity (false positive) of human papillomavirus (HPV) DNA testing for the screening and diagnosis of cervical samples. Therefore, we evaluated the performance of the HPV OncoCheck assay, which is an HPV E6/E7 mRNA-based assay, for the detection of 16 high-risk (HR)-HPVs including HPV 16 and HPV 18 genotypes in cervical samples using multiplex reverse transcriptase-quantitative PCR. In the present study, the analytical performance of the assay was evaluated using 16 HPV single strand DNAs...
December 31, 2018: Experimental and Molecular Pathology
Akhil Katuri, Joseph L Bryant, Dhruvil Patel, Vivek Patel, Sanketh Andhavarapu, Girma Asemu, Harry Davis, Tapas K Makar
Human immunodeficiency virus associated nephropathy (HIVAN) is a unique form of a renal parenchymal disorder. This disease and its characteristics can be accredited to incorporation of DNA and mRNA of human immunodeficiency virus type 1 into the renal parenchymal cells. A proper understanding of the intricacies of HIVAN and the underlying mechanisms associated with renal function and disorders is vital for the potential development of a reliable treatment for HIVAN. Specifically, the renal tubule segment of the kidney is characterized by its transport capabilities and its ability to reabsorb water and salts into the blood...
December 31, 2018: Experimental and Molecular Pathology
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