Fei Fang, Shiding Li, Hao Sun, Yao Fu, Chunyi Shao
Limbal stem cell deficiency (LSCD) is a clinically treatment-challenging eye disease caused by damage to limbal stem cells (LSCs). Currently, the international consensus classifies LSCD into three clinical stages based on the disease severity. However, no existing animal models attempt to replicate the varying degrees of LSCD observed in clinical cases. The present study demonstrates an easy-to-create, reproducible, and reliable mouse model of graded LSCD. To achieve mild, moderate, or severe LSCD, filter paper rings with a variety of central angles (90, 180, or 270 degrees) are utilized to deliver alkali burn to different sizes of limbal area (1, 2, or 3 quarters)...
May 23, 2024: Experimental Eye Research
Xueping Wu, Jia Liang, Jinfeng Liu, Yijia Huang, Liyun Zhang, Xin Liu, Junhong Guo, Min Zhang, Yudong Chen, Jiantao Wang
Transforming growth factor-β2 (TGF-β2) induced fibrogenic changes in human trabecular meshwork (HTM) cells have been implicated in trabecular meshwork (TM) damage and intraocular pressure (IOP) elevation in primary open-angle glaucoma (POAG) patients. Silibinin (SIL) exhibited anti-fibrotic properties in various organs and tissues. This study aimed to assess the effects of SIL on the TGF-β2-treated HTM cells and to elucidate the underlying mechanisms. Our study found that SIL effectively inhibited HTM cell proliferation, attenuated TGF-β2-induced cell migration, and mitigated TGF-β2-induced reorganization of both actin and vimentin filaments...
May 22, 2024: Experimental Eye Research
Ayelen Inda, Sofía Martinez, Carolina Bessone, Maximiliano Rios, Mario Guido, Rocío Herrero-Vanrell, Jose Domingo Luna, Daniel Allemandi, Soledad Ravetti, Daniela Quinteros
Neurodegenerative pathologies affecting the posterior segment of the eye, are characterized by being devastating and responsible for the majority of visual dysfunctions worldwide. These diseases are primarily degenerative, progressing chronically, and can inflict gradual harm to the optic nerve, retinal ganglion cells (RGC), photoreceptors, and other retinal cells. This retinal damage leads to a progressive loss of vision, marking these conditions as a significant health concern worldwide. The intravitreal administration of the phytochemical Carvacrol (CAR) is expected to demonstrate a neuroprotective and antiapoptotic effect on retinal cells, with a specific focus on RGC...
May 22, 2024: Experimental Eye Research
Xiu Wang, Na Li, Jiawen Zhang, Jiali Wang, Yankai Wei, Jun Yang, Deming Sun, Lin Liu, Hong Nian, Ruihua Wei
Sjögren's syndrome (SS) dry eye can cause ocular surface inflammation and lacrimal gland (LG) damage, leading to discomfort and potential vision problems. The existing treatment options for SS dry eye are currently constrained. We investigated the possible therapeutic effect and the underlying mechanism of AS101 in autoimmune dry eye. AS101 was injected subconjunctivally into a rabbit model of autoimmune dacryoadenitis and its therapeutic effects were determined by evaluating clinical and histological scores...
May 21, 2024: Experimental Eye Research
Samira Izuagbe, Joyita Roy, Amjad Chatila, Le Quynh Hoang, Vicki Ea, Bhavya Vaish, Cynthia M Co, Aaron Ly, Hongli Wu, Liping Tang
Posterior Capsule Opacification (PCO), the most frequent complication of cataract surgery, is caused by the infiltration and proliferation of lens epithelial cells (LECs) at the interface between the intraocular lens (IOL) and posterior lens capsule (PLC). According to the "no space, no cells, no PCO" theory, high affinity (or adhesion force) between the IOL and PLC would decrease the IOL: PLC interface space, hinder LEC migration, and thus reduce PCO formation. To test this hypothesis, an in vitro hemisphere-shaped simulated PLC (sPLC) was made to mimic the human IOL: PLC physical interactions and to assess their influence on LEC responses...
May 21, 2024: Experimental Eye Research
Katelyn K Dilley, Karthik R Prasad, Theodore V Nguyen, Anna Stokolosa, Pamela A Borden, J Martin Heur, Sehwan Kim, Michael G Hill, Brian J F Wong
Refractive errors remain a global health concern, as a large proportion of the world's population is myopic. Current ablative approaches are costly, not without risks, and not all patients are candidates for these procedures. Electromechanical reshaping (EMR) has been explored as a viable cost-effective modality to directly shape tissues, including cartilage. In this study, stromal collagen structure and fibril orientation was examined before and after EMR with second-harmonic generation microscopy (SHG), a nonlinear multiphoton imaging method that has previously been used to study native corneal collagen with high spatial resolution...
May 21, 2024: Experimental Eye Research
Baiyi Li, Mi Zhang, Shuang Chen, Chuan Zhao, Xiaorong Li, Xiaomin Zhang
Non-infectious uveitis is an intraocular autoimmune disease mainly characterized by immune dysregulation of the eye, which may cause blindness if not well treated. Interleukin 10 (IL-10) is a potent cytokine with multiple immunoregulatory functions. However, it's in vivo activity is unstable owing to its inherent protein instability and short plasma half-life. Therefore, our previous research tried to establish IL-10-overexpressing MSC-sEVs (sEVs-IL10) using lentiviral transfection. While this approach indeed improved drug delivery, it also suffered from tedious procedures and limited loading efficiency...
May 18, 2024: Experimental Eye Research
Mingjun Tang, Liuxueying Zhong, Huifeng Rong, Kaijing Li, Meifang Ye, Jingyi Peng, Jian Ge
Gene therapy is one of the strategies that may reduce or reverse progressive neurodegeneration in retinal neurodegenerative diseases. However, efficiently delivering transgenes to retinal ganglion cells (RGCs) remains hard to achieve. In this study, we innovatively investigated transduction efficiency of adeno-associated virus (AAV)-PHP.eB in murine RGCs by retro-orbital venous sinus injection. Five doses of AAV-PHP.eB-EGFP were retro-orbitally injected in venous sinus in adult C57/BL6J mice. Two weeks after administration, RGCs transduction efficiency was quantified by retinal flat-mounts and frozen section co-labeling with RGCs marker Rbpms...
May 17, 2024: Experimental Eye Research
Haoyu Zuo, Wenjuan Han, Keling Wu, Haohan Yang, Huiying Song, Zirong Zhang, Yuhua Lai, Zhongshu Pan, Weihua Li, Ling Zhao
Müller glia and microglia are capable of phagocytosing fragments of retinal cells in response to retinal injury or degeneration. However, the direct evidence for their mutual interactions between Müller glia and microglia in the progression of Retinal Degeneration (RD) remains largely unclear. This study aims to construct a progressive RD mouse model and investigate the activated pattern of Müller glia and the interplay between Müller glia and microglia in the early stage or progression of RD...
May 17, 2024: Experimental Eye Research
Chia-Yu Chang, Steven S-S Wang, You-Ren Lai, Won-Gun Koh, Josephine W Wu, Yi-Hui Chiang
Drugs that can treat one disease may either be detrimental or beneficial toward another due to possible cross-interactions. Therefore, care in choosing a suitable drug for patients with multiple diseases is crucial in successful patient management. This study explores several currently available ophthalmic drugs used to treat common ocular diseases to understand how they can affect the amyloidogenesis of a transforming growth factor β-induced protein (TGFBIp) peptide fragment found in abundance in the corneal protein aggregation deposits of lattice corneal dystrophy (LCD) patients...
May 16, 2024: Experimental Eye Research
Reza A Badian, Neil Lagali
There has been a growing application of in vivo confocal microscopy (IVCM) in the examination of corneal microstructure, including different corneal layers and corneal nerve fibers in health and in pathological conditions. Corneal nerves forming the sub-basal nerve plexus (SBNP) beneath the corneal basal epithelial cell layer in particular have been intensively researched in health and disease as a marker for corneal neurophysioanatomical and degenerative changes. One intriguing feature in the SBNP that is found inferior to the corneal apex, is a whorl-like pattern (or vortex) of nerves, which represents an anatomical landmark...
May 15, 2024: Experimental Eye Research
Jiayu Zou, Binrong Wu, Yan Tao, Zuimeng Liu, Huanyu Zhao, Pin Wang, Yuanbo Liang, Jia Qu, Shaodan Zhang
Subconjunctival fibrosis is the major cause of failure in both conventional and modern minimally invasive glaucoma surgeries (MIGSs) with subconjunctival filtration. The search for safe and effective anti-fibrotic agents is critical for improving long-term surgical outcomes. In this study, we investigated the effect of inhibiting the rapamycin-insensitive mTORC1/4E-BP1 axis on the transforming growth factor-beta 1(TGF-β1)-induced fibrotic responses in human Tenon's fibroblasts (HTFs), as well as in a rat model of glaucoma filtration surgery (GFS)...
May 13, 2024: Experimental Eye Research
Lanfang Sun, Yixin Cen, Xiaojiang Liu, Jinfei Wei, Xiaoyu Ke, Yanan Wang, Qianling Liao, Mengchun Chang, Meng Zhou, Wencan Wu
Optic nerve injuries are severely disrupt the structural and functional integrity of the retina, often leading to visual impairment or blindness. Despite the profound impact of these injuries, the molecular mechanisms involved remain poorly understood. In this study, we performed a comprehensive whole-transcriptome analysis of mouse retina samples after optic nerve crush (ONC) to elucidate changes in gene expression and regulatory networks. Transcriptome analysis revealed a variety of molecular alterations, including 256 mRNAs, 530 lncRNAs, and 37 miRNAs, associated with metabolic, inflammatory, signaling, and biosynthetic pathways in the injured retina...
May 13, 2024: Experimental Eye Research
Yinuo Wen, Zhongxing Chen, Colm McAlinden, Xingtao Zhou, Jinhai Huang
Corneal neovascularization (CoNV) is a vision-threatening ocular disease commonly secondary to infectious, inflammatory, and traumatic etiologies. Slit lamp photography, in vivo confocal microscopy, angiography, and optical coherence tomography angiography (OCTA) are the primary diagnostic tools utilized in clinical practice to evaluate the vasculature of the ocular surface. However, there is currently a dearth of comprehensive literature that reviews the advancements in imaging technology for CoNV administration...
May 13, 2024: Experimental Eye Research
Mengyuan Xie, Meizhong Liao, Sihui Chen, Deliang Zhu, Qiaolang Zeng, Peiyuan Wang, Caiying Su, Ruiling Lian, Jiansu Chen, Jun Zhang
The corneal epithelium, located as the outermost layer of the cornea, is inherently susceptible to injuries that may lead to corneal opacities and compromise visual acuity. Rapid restoration of corneal epithelial injury is crucial for maintaining the transparency and integrity of the cornea. Cell spray treatment emerges as an innovative and effective approach in the field of regenerative medicine. In our study, a cell spray printing platform was established, and the optimal printing parameters were determined to be a printing air pressure of 5 PSI (34...
May 13, 2024: Experimental Eye Research
Zhe-Qing Peng, Xiao-Hui Guan, Zhen-Ping Yu, Jie Wu, Xin-Hao Han, Ming-Hui Li, Xin-Hui Qu, Zhi-Ping Chen, Xiao-Jian Han, Xiao-Yu Wang
Age-related macular degeneration (AMD) is the leading cause of vision loss among the elderly, which is primarily attributed to oxidative stress-induced damage to the retinal pigment epithelium (RPE). Human amniotic mesenchymal stem cells (hAMSC) were considered to be one of the most promising stem cells for clinical application due to their low immunogenicity, tissue repair ability, pluripotent potential and potent paracrine effects. The conditional medium (hAMSC-CM) and exosomes (hAMSC-exo) derived from hAMSC, as mediators of intercellular communication, play an important role in the treatment of retinal diseases, but their effect and mechanism on oxidative stress-induced retinal degeneration are not explored...
May 8, 2024: Experimental Eye Research
Marzieh Tahmasebi Sarvestani, Glyn Chidlow, John P Wood, Robert J Casson
Neovascular age-related macular degeneration, also known as exudative or wet age-related macular degeneration, is the leading cause of blindness in the developed world. Photobiomodulation has the potential to target the up-stream hypoxic and pro-inflammatory drivers of choroidal neovascularization. This study investigated whether photobiomodulation attenuates characteristic pathological features of choroidal neovascularization in a rodent model. Experimental choroidal neovascularization was induced in Brown Norway rats with laser photocoagulation...
May 4, 2024: Experimental Eye Research
Mason Posner, Taylor Garver, Taylor Kaye, Stuart Brdicka, Madison Suttle, Bryce Patterson, Dylan R Farnsworth
The vertebrate eye lens is an unusual organ in that most of its cells lack nuclei and the ability to replace aging protein. The small heat shock protein α-crystallins evolved to become key components of this lens, possibly because of their ability to prevent aggregation of aging protein that would otherwise lead to lens opacity. Most vertebrates express two α-crystallins, αA- and αB-crystallin, and mutations in each are linked to human cataract. In a mouse knockout model only the loss of αA-crystallin led to early-stage lens cataract...
May 3, 2024: Experimental Eye Research
Ann Mozzer, Ian Pitha
In glaucoma, scleral fibroblasts are exposed to IOP-associated mechanical strain and elevated TGFβ levels. These stimuli, in turn, lead to scleral remodeling. Here, we examine the scleral fibroblast migratory and transcriptional response to these stimuli to better understand mechanisms of glaucomatous scleral remodeling. Human peripapillary scleral (PPS) fibroblasts were cultured on parallel grooves, treated with TGFβ (2 ng/ml) in the presence of vehicle or TGFβ signaling inhibitors, and exposed to uniaxial strain (1 Hz, 5%, 12-24 hours)...
April 30, 2024: Experimental Eye Research
William E Myles, Yusupjan Abdulla, Sally Anne McFadden
A-scan ultrasonography enables precise measurement of internal ocular structures. Historically, its use has underpinned fundamental studies of eye development and aberrant eye growth in animal models of myopia; however, the procedure typically requires anaesthesia. Since anaesthesia affects intra-ocular pressure (IOP), we investigated changes in internal ocular structures with isoflurane exposure and compared measurements with those taken in awake animals using optical coherence tomography (OCT). Continuous A-scan ultrasonography was undertaken in tri-coloured guinea pigs aged 21 (n = 5), 90 (n = 5) or 160 (n = 5) days while anaesthetised (up to 36 mins) with isoflurane (5% in 1...
April 27, 2024: Experimental Eye Research
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