journal
https://read.qxmd.com/read/38537669/single-cell-profiling-transcriptomic-reveals-cellular-heterogeneity-and-cellular-crosstalk-in-choroidal-neovascularization-model
#1
JOURNAL ARTICLE
Ming Tong, Yun Bai, Xiaoyan Han, Lingjie Kong, Ling Ren, Linyu Zhang, Xiumiao Li, Jin Yao, Yan Biao
Choroidal neovascularization (CNV) is a hallmark of neovascular age-related macular degeneration (nAMD) and a major contributor to vision loss in nAMD cases. However, the identification of specific cell types associated with nAMD remains challenging. Herein, we performed single-cell sequencing to comprehensively explore the cellular diversity and understand the foundational components of the retinal pigment epithelium (RPE)/choroid complex. We unveiled 10 distinct cell types within the RPE/choroid complex. Notably, we observed significant heterogeneity within endothelial cells (ECs), fibroblasts, and macrophages, underscoring the intricate nature of the cellular composition in the RPE/choroid complex...
March 25, 2024: Experimental Eye Research
https://read.qxmd.com/read/38527580/local-administration-of-myeloid-derived-suppressor-cells-prevents-progression-of-immune-mediated-dry-eye-disease
#2
JOURNAL ARTICLE
Pier Luigi Surico, Seokjoo Lee, Rohan Bir Singh, Amirreza Naderi, Shilpy Bhullar, Tomas Blanco, Yihe Chen, Reza Dana
Myeloid derived suppressor cells (MDSCs) are a heterogenous population of immature hematopoietic precursors with known immunoregulatory functions. The immunosuppressive role of MDSCs has been highlighted in several inflammatory ophthalmic disorders; however, their therapeutic application in suppressing the immune-mediated changes in dry eye disease (DED) have not been studied. We observed significant reduction in antigen presenting cell (APC) frequencies and their maturation in the presence of MDSCs. Moreover, co-culturing MDSCs with T helper 17 cells (Th17) resulted in reduced Th17 frequencies and their IL-17 expression...
March 23, 2024: Experimental Eye Research
https://read.qxmd.com/read/38522635/pedf-derived-peptide-protects-against-amyloid-%C3%AE-toxicity-in-vitro-and-prevents-retinal-dysfunction-in-rats
#3
JOURNAL ARTICLE
Amanda Qarawani, Efrat Naaman, Rony Ben- Tzvi Elimelech, Michal Harel, Chen Itzkovich, Shadi Safuri, Nitsan Dahan, Jack Henkin, Shiri Soudry
Amyloid-beta (Aβ), a family of aggregation-prone and neurotoxic peptides, has been implicated in the pathophysiology of age-related macular degeneration (AMD). We have previously shown that oligomeric and fibrillar species of Aβ42 exerted retinal toxicity in rats, but while the consequences of exposure to amyloid were related to intracellular effects, the mechanism of Aβ42 internalization in the retina is not well characterized. In the brain, the 67 kDa laminin receptor (67LR) participates in Aβ-related neuronal cell death...
March 22, 2024: Experimental Eye Research
https://read.qxmd.com/read/38514024/photoreceptor-deficits-appear-at-eye-opening-in-rs1-mutant-mouse-models-of-x-linked-retinoschisis
#4
JOURNAL ARTICLE
Matthew J Tarchick, Craig Beight, Paul B Bonezzi, Neal S Peachey, Jordan M Renna
X-linked retinoschisis is an early onset degenerative retinal disease characterized by cystic lesions in the middle layers of the retina. These structural changes are accompanied by a loss of visual acuity and decreased contrast sensitivity. XLRS is caused by mutations in the gene Rs1 which encodes the secreted protein Retinoschisin 1. Young Rs1-mutant mouse models develop key hallmarks of XLRS including intraretinal schisis and abnormal electroretinograms. The ERG comprises activity of multiple cellular generators, and it is not known how and when each of these is impacted in Rs1 mutant mice...
March 19, 2024: Experimental Eye Research
https://read.qxmd.com/read/38514023/role-of-mrna-binding-proteins-in-retinal-neovascularization
#5
REVIEW
Pei Lin, Wenye Cao, Xuemei Chen, Ningzhi Zhang, Yiqiao Xing, Ning Yang
Retinal neovascularization (RNV) is a pathological process that primarily occurs in diabetic retinopathy, retinopathy of prematurity, and retinal vein occlusion. It is a common yet debilitating clinical condition that culminates in blindness. Urgent efforts are required to explore more efficient and less limiting therapeutic strategies. Key RNA-binding proteins (RBPs), crucial for post-transcriptional regulation of gene expression by binding to RNAs, are closely correlated with RNV development. RBP-RNA interactions are altered during RNV...
March 19, 2024: Experimental Eye Research
https://read.qxmd.com/read/38494102/mesenchymal-stem-cell-based-adjunctive-therapy-for-pseudomonas-aeruginosa-induced-keratitis-a-proof-of-concept-in-vitro
#6
JOURNAL ARTICLE
Sreya Mitra, Vasudeva Tati, Prabhudatta Das, Joveeta Joseph, Bhupesh Bagga, Sachin Shukla
PURPOSE: Pseudomonas aeruginosa-induced keratitis is one of the most severe and challenging forms of corneal infection, owing to its associated intense inflammatory reactions leading to corneal necrosis and dense corneal scar with loss of vision. Since mesenchymal stem cells (MSCs) are reported to possess antimicrobial and immunomodulatory properties, they can be tested as an adjuvant treatment along with the antibiotics which are the current standard of care. This study aims to investigate the anti-bacterial and immunomodulatory roles of human bone marrow MSC-derived conditioned medium (MSC-CM) in P...
March 15, 2024: Experimental Eye Research
https://read.qxmd.com/read/38490292/screening-and-evaluation-of-antioxidants-for-retinal-pigment-epithelial-cell-protection-l-ergothioneine-as-a-novel-therapeutic-candidate-through-nrf2-activation
#7
JOURNAL ARTICLE
Sijie Gu, Siqi Wu, Zesong Lin, Zhuo Han, Kunlun Mo, Huaxing Huang, Mingsen Li, Gen Li, Hong Ouyang, Li Wang
The continual exposure of retinal tissues to oxidative stress leads to discernible anatomical and physiological alterations. Specifically, the onslaught of oxidative damage escalates the irreversible death of retinal pigmented epithelium (RPE) cells, pinpointed as the fundamental pathological event in dry age-related macular degeneration (AMD). There is a conspicuous lack of effective therapeutic strategies to counteract this degenerative process. This study screened a library of antioxidants for their ability to protect RPE cells against oxidative stress and identified L-ergothioneine (EGT) as a potent cytoprotective agent...
March 13, 2024: Experimental Eye Research
https://read.qxmd.com/read/38479725/modeling-autosomal-dominant-retinitis-pigmentosa-by-using-patient-specific-retinal-organoids-with-a-class-3-rho-mutation
#8
JOURNAL ARTICLE
Xiao Lin, Zhuo-Lin Liu, Xiao Zhang, Wen Wang, Zhi-Qin Huang, Shu-Ning Sun, Zi-Bing Jin
Rhodopsin-mediated autosomal dominant retinitis pigmentosa (RHO-adRP) causes progressive vision loss and is potentially incurable, accounting for 25% of adRP cases. Studies on RHO-adRP mechanism were at large based on the biochemical and cellular properties, especially class-3. Nonetheless, the absence of an appropriate model for class-3 RHO-adRP has impeded comprehensive exploration. Here, induced pluripotent stem cells (iPSCs) were generated from a healthy control and two sibling RP patients with the same point mutation, c...
March 11, 2024: Experimental Eye Research
https://read.qxmd.com/read/38479724/rho-kinase-inhibitor-alleviates-cd4-t-cell-mediated-corneal-graft-rejection-by-modulating-its-stat3-and-stat5-activation
#9
JOURNAL ARTICLE
Shang Li, Peng Zhang, Ao Li, Jiayu Bao, Zhiqiang Pan, Ying Jie
Penetrating keratoplasty remains the most common treatment to restore vision for corneal diseases. Immune rejection after corneal transplantation is one of the major causes of graft failure. In recent years, Rho-associated protein kinase (ROCK) inhibitors have been found to be associated with the activation of the STATs pathway and are widely studied in autoimmune diseases. Therefore, it may be possible that the ROCK inhibitors also participate in the local and systemic immune regulation in corneal transplantation through activation of the STATs pathway and affect the CD4+ T cell differentiation...
March 11, 2024: Experimental Eye Research
https://read.qxmd.com/read/38467175/stop-codon-variant-in-efemp1-is-associated-with-primary-open-angle-glaucoma-due-to-impaired-regulation-of-aqueous-humor-outflow
#10
JOURNAL ARTICLE
Junkai Tan, Suping Cai, Xiaolin Luo, Qiang Li, Yanbing Chen, Zijie Chen, Yukai Mao, Guo Liu, Mingming Yang, Xuyang Liu
It is known that the actin cytoskeleton and its associated cellular interactions in the trabecular meshwork (TM) and juxtacanalicular tissues mainly contribute to the formation of resistance to aqueous outflow of the eye. Fibulin-3, encoded by EFEMP1 gene, has a role in extracellular matrix (ECM) modulation, and interacts with enzymatic ECM regulators, but the effects of fibulin-3 on TM cells has not been explored. Here, we report a stop codon variant (c.T1480C, p.X494Q) of EFEMP1 that co-segregates with primary open angle glaucoma (POAG) in a Chinese pedigree...
March 11, 2024: Experimental Eye Research
https://read.qxmd.com/read/38467176/optical-development-in-the-murine-eye-lens-of-accelerated-senescence-prone-samp8-and-senescence-resistant-samr1-strains
#11
JOURNAL ARTICLE
Kehao Wang, Yutian Pu, Leran Chen, Masato Hoshino, Kentaro Uesugi, Naoto Yagi, Xiaoyong Chen, Yuu Usui, Akira Hanashima, Ken Hashimoto, Satoshi Mohri, Barbara K Pierscionek
The eye lens is responsible for focusing objects at various distances onto the retina and its refractive power is determined by its surface curvature as well as its internal gradient refractive index (GRIN). The lens continues to grow with age resulting in changes to the shape and to the GRIN profile. The present study aims to investigate how the ageing process may influence lens optical development. Murine lenses of accelerated senescence-prone strain (SAMP8) aged from 4 to 50 weeks; senescence-resistant strain (SAMR1) aged from 5 to 52 weeks as well as AKR strain (served as control) aged from 6 to 70 weeks were measured using the X-ray interferometer at the SPring-8 synchrotron Japan within three consecutive years from 2020 to 2022...
March 9, 2024: Experimental Eye Research
https://read.qxmd.com/read/38467174/drp1-dependent-mitochondrial-fragmentation-mediates-photoreceptor-abnormalities-in-type-1-diabetic-retina
#12
JOURNAL ARTICLE
Shuyu Tang, Mengling Huang, Ruixuan Wang, Ming Li, Ning Dong, Ronghan Wu, Zailong Chi, Ling Gao
Recent studies have highlighted that retinal neurodegeneration precedes microvascular changes in diabetic retinopathy (DR), but the specific mechanisms remain unclear. Given the pivotal role of dysfunctional mitochondria and oxidative stress in early DR, our objective was to observe mitochondria-related alterations in the neural retina of type one diabetic mellitus mice with no evidence of DR (T1DM-NDR). We aimed to identify the key mitochondrial-related proteins contributing to mitochondrial injury. Our study revealed that T1DM-NDR mice exhibited outer retina thinning, including the ellipsoid zone, inner segment, and outer segment...
March 9, 2024: Experimental Eye Research
https://read.qxmd.com/read/38460719/sphingolipid-biosynthetic-inhibitor-l-cycloserine-prevents-oxidative-stress-mediated-death-in-an-in-vitro-model-of-photoreceptor-derived-661w-cells
#13
JOURNAL ARTICLE
Faiza Tahia, Sandip K Basu, Amanda Prislovsky, Koushik Mondal, Dejian Ma, Harry Kochat, Kennard Brown, Daniel J Stephenson, Charles E Chalfant, Nawajes Mandal
Oxidative stress plays a pivotal role in the pathogenesis of several neurodegenerative diseases. Retinal degeneration causes irreversible death of photoreceptor cells, ultimately leading to vision loss. Under oxidative stress, the synthesis of bioactive sphingolipid ceramide increases, triggering apoptosis in photoreceptor cells and leading to their death. This study investigates the effect of L-Cycloserine, a small molecule inhibitor of ceramide biosynthesis, on sphingolipid metabolism and the protection of photoreceptor-derived 661 W cells from oxidative stress...
March 7, 2024: Experimental Eye Research
https://read.qxmd.com/read/38453040/intraocular-pressure-across-the-lifespan-of-tg-myoc-y437h-mice
#14
JOURNAL ARTICLE
Xiaoyan Zhang, Gaiping Xi, Pengchao Feng, Cong Li, Markus H Kuehn, Wei Zhu
Transgenic C57BL/6 mice expressing human myocilinY437 (Tg-MYOCY437H ) are a well-established model for primary open-angle glaucoma (POAG). While the reduced trabecular meshwork (TM) cellularity due to severe endoplasmic reticulum (ER) stress has been characterized as the etiology of this model, there is a limited understanding of how glaucomatous phenotypes evolve over the lifespan of Tg-MyocY437H mice. In this study, we compiled the model's intraocular pressure (IOP) data recorded in our laboratory from 2017 to 2023 and selected representative eyes to measure the outflow facility (Cr ), a critical parameter indicating the condition of the conventional TM pathway...
March 5, 2024: Experimental Eye Research
https://read.qxmd.com/read/38453039/oleic-acid-induces-lipogenesis-and-nlrp3-inflammasome-activation-in-organotypic-mouse-meibomian-gland-and-human-meibomian-gland-epithelial-cells
#15
JOURNAL ARTICLE
Ren Liu, Mengqian Tu, Jianwen Xue, Bing Xiao, Jing Li, Lingyi Liang
The accumulation of oleic acid (OA) in the meibum from patients with meibomian gland dysfunction (MGD) suggests that it may contribute to meibomian gland (MG) functional disorder, as it's a potent stimulator of acne-related lipogenesis and inflammation in sebaceous gland. Therefore, we investigate whether OA induces lipogenesis and inflammasome activation in organotypic cultured mouse MG and human meibomian gland epithelial cells (HMGECs). Organotypic cultured mouse MG and HMGECs were exposed to OA or combinations with specific AMPK agonists 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR)...
March 5, 2024: Experimental Eye Research
https://read.qxmd.com/read/38453038/targeting-mechanics-induced-trabecular-meshwork-dysfunction-through-yap-tgf%C3%AE-ameliorates-high-myopia-induced-ocular-hypertension
#16
JOURNAL ARTICLE
Qianwen Bu, Hai Zhu, Guangliang Cao, Ganyu Gong, Ying Su, Qingshu Ge, Wei Zhu, Zongyi Li, Xiaojing Pan
High myopia is a risk factor for primary open angle glaucoma (POAG). The pathological mechanism of high myopia induced POAG occurrence is not fully understood. In this study, we successfully established the guinea pig model of ocular hypertension with high myopia, and demonstrated the susceptibility of high myopia for the occurrence of microbead-induced glaucoma compared with non-myopia group and the effect of YAP/TGF-β signaling pathway in TM pathogenesis induced by high myopia. Moreover, we performed stretching treatment on primary trabecular meshwork (TM) cells to simulate the mechanical environment of high myopia...
March 5, 2024: Experimental Eye Research
https://read.qxmd.com/read/38453037/analysis-of-the-mucosal-chemokines-ccl28-cxcl14-and-cxcl17-in-dry-eye-disease-an-in-vitro-and-clinical-investigation
#17
JOURNAL ARTICLE
Alfredo Domínguez-López, Marta Blanco-Vázquez, Andrés Ángel Calderón-García, Carmen García-Vázquez, María J González-García, Margarita Calonge, Amalia Enríquez-de-Salamanca
Mucosal chemokines have antimicrobial properties and play an important role in mucosal immunity. However, little is known about their expression on the ocular surface. This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under in vitro dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients. Human corneal epithelial cells (HCE) and immortalized human conjunctival epithelial cells (IM-HConEpiC) were incubated under hyperosmolar (400-500 mOsM) or inflammatory (TNF-α 25 ng/mL) conditions for 6 h and 24 h to measure CCL28, CXCL14, and CXCL17 gene expression by RT-PCR and their secretion by immunobead-based analysis (CCL28, CXCL14) and ELISA (CXCL17)...
March 5, 2024: Experimental Eye Research
https://read.qxmd.com/read/38430983/characterization-of-lncrna-and-mrna-profiles-in-ciliary-body-in-experimental-myopia
#18
JOURNAL ARTICLE
Shanshan Wu, Jiawen Hao, Dadong Guo, Zhongyu Ma, Qiuxin Wu, Ming Zhang, Hongsheng Bi
Currently, researchers have mainly focused on the role of the tissues of the posterior segment of the eyes in the development of myopia. However, the ciliary body, an anterior ocular tissue that contracts to initiate the process of accommodation, may also play an important role in the progression of myopia due to the increased demand for near work. In the present study, we established a lens-induced myopia (LIM) animal model in guinea pigs and investigated the molecular changes in the ciliary body associated with the development of myopia based on RNA sequencing...
February 29, 2024: Experimental Eye Research
https://read.qxmd.com/read/38428474/a-minimally-invasive-method-for-diagnosing-diseases-of-the-eye
#19
JOURNAL ARTICLE
Vera B Morhenn, Jonathan N Mansbridge, Harvey Fishman
No abstract text is available yet for this article.
February 28, 2024: Experimental Eye Research
https://read.qxmd.com/read/38423204/causal-associations-of-refractive-error-and-early-age-related-macular-degeneration-a-mendelian-randomization-study
#20
JOURNAL ARTICLE
Lingling Wang, Wenlong Wei, YongJi Zhao, Sixi Chen, Dongjing Wu, Mengjun Tu
This study aims to determine the risk associated with early age-related macular degeneration (AMD) due to refractive errors (RE) using an analysis of genome-wide association study (GWAS) data through the two-sample Mendelian randomization approach. Single-nucleotide polymorphisms (SNPs) linked to refractive errors (RE) were obtained from numerous GWAS studies involving individuals of European descent. The data for early AMD was obtained from a diverse, multiethnic GWAS meta-analysis that included 105,248 participants (14,034 cases and 91,214 controls)...
February 27, 2024: Experimental Eye Research
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