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Acta Virologica

D Risco, P Gonçalves, R Cerrato, P Fernández-Llario
Wild boar is an important reservoir of Aujeszky's disease virus (ADV). There is concern that transmission of this virus from wild boar to domestic pigs is possible. The aim of this work was to compare the antibody response produced by single dose of a gE-deleted ADV vaccine in wild boar to revaccinated animals, to assess if simple single-dose vaccination plans should be examined as a possible control measure against ADV in wild boar. Twenty-five wild boar (ages ranging between 2.5 to 5 months) were included in this study and distributed in three different groups: a control group (n = 5), a single-dose group (10 animals vaccinated only with one dose (day 0)) and a revaccinated group [10 animals vaccinated (day 0) and revaccinated (day 28)]...
2018: Acta Virologica
L Yu, B Sun, X Liu, J He, H Lou, X Chen, F Tong, L Wei, Y Zhang, W Wei
High-risk human papillomavirus (HPV) is an important pathogenic factor for cervical cancer and understanding the mechanism of HPV gene transcription is important for the prevention and therapy of HPV related cervical cancer. This study aimed to investigate the role of nitric oxide (NO) in the regulation of HPV gene transcription. SiHa cells containing integrated HPV16 were treated with NO donor DETA-NO and cell proliferation and cytotoxicity were determined. HPV gene transcription was detected by real-time PCR...
2018: Acta Virologica
P Ma, X-X Ma, Q-Y Chang, L-J Li, Y-N Wang, Y-P Feng, Z-R Ma, J-H Zhou
Internal ribosomal entry site (IRES) functions as a cis-acting RNA element, which drives an alternative and cap-independent translation initiation pathway. Currently, there are few studies on effects of nucleotide usages at key nucleotide positions +4 and -3 flanking start codon mediated by IRES of hepatitis C virus (HCV). Herein, we focus on the effect of nucleotide usages at -3 and +4 positions mediated by HCV IRES. The nucleotide contexts flanking AUG start codon employed by HCV IRES is firstly analyzed...
2018: Acta Virologica
W Liu, H Xiao, S Wu, H Liu, B Luo
Microtubule-associated protein 9 (MAP9) is a mitosis-associated protein involved in bipolar spindle assembly. Following DNA damage, MAP9 stabilizes p53 via p300 and MDM2 (mouse double minute-2 homolog). The dysregulation of MAP9 was considered to be associated with tumorigenesis. Single nucleotide polymorphisms (SNPs) in key genes governing mitosis may particularly increase susceptibility to gastric carcinoma (GC). Our study demonstrated that the CC homozygous genotype of SNP rs1058992 located in the MAP9 gene was significantly correlated with EBV-associated GC (EBVaGC) in a recessive genetic model (OR = 2...
2018: Acta Virologica
M Labudová, F Čiampor, S Pastoreková, J Pastorek
Lymphocytic choriomeningitis virus (LCMV) can establish in its host a persistent infection, without any prominent symptoms. Even during this infection, when the infectious virions are not released, the virus still disseminates effectively. A very effective and fast way of infection of neighboring cells utilized by many viruses is cell-to-cell transmission. Viruses use different ways of cell-to-cell spread through the extracellular space or by intracellular means through different protrusions. We have found that LCMV strain MX may use three different types of cell-to-cell transport...
2018: Acta Virologica
M Tu, P Liu, F Liu, M Wang, R Jia, D Zhu, M Liu, K Sun, Q Yang, Y Wu, X Chen, A Cheng, S Chen
Goose parvovirus (GPV) is a highly contagious and lethal disease in goslings and Muscovy ducklings, and is of concern to the waterfowl industry. With the aim of comparing the cellular immunogenicity of three capsid proteins of GPV, plasmids of pcDNA3.1(+)-VP1, pcDNA3.1(+)-VP2, and pcDNA3.1(+)-VP3 were constructed, and the recombinant protein VPs were expressed using an eukaryotic expression system. We detected the levels of immune-related genes (CD4, CD8α, IL-1β, IL-6, IFNα, IFNγ, and IFNλ) in both goose embryo fibroblasts (GEF) and goose peripheral blood mononuclear cells (PBMCs) cellular models...
2018: Acta Virologica
F Zúniga-Navarrete, G Flores-Ramirez, M Quevedo-Díaz, L Škultéty
Coxiella burnetii is an intracellular pathogenic bacterium and etiological agent of Q fever in humans. Recently, the bacterium has been set free from the strictly intracellular condition by successful cultivation in acidified citrate cysteine medium. Here, we report a bacterial cell counting method that allows rapid quantification of the absolute or relative number of live cells of C. burnetii in a high throughput manner. The method utilizes TaqMan-based quantitative polymerase chain reaction (qPCR) targeting a single dotA gene for determination of genome equivalent (GE) presented either as DNA or complementary DNA (cDNA) synthesized via reverse transcription...
2018: Acta Virologica
C Sugita, K Shin, H Wakabayashi, R Tsuhako, H Yoshida, W Watanabe, M Kurokawa
Hypothiocyanite (OSCN-) is a natural component of human saliva and is produced by the lactoperoxidase (LPO)/thiocyanate/hydrogen peroxide (H2O2) system. OSCN- has been previously shown to exhibit antiviral activity against influenza viruses (IFV) A/H1N1/2009 and A/H1N2/2009 in vitro as well as antimicrobial and antifungal activities. We elucidated the antiviral activity of OSCN- against both IFV types A and B and the mode of its antiviral action. OSCN- was produced constantly at 900 ± 200 μmol/l in Na3PO4 buffer solution containing NaSCN and LPO in the presence of H2O2 as an original OSCN- solution...
2018: Acta Virologica
F Sobhanimonfared, T Bamdad, Z A Sadigh, H R Nikoo, H Choobin
Oncolytic virotherapy offers the potential to treat tumors both as a single agent and in combination with conventional therapies such as chemotherapy and immunological therapy. Here, we describe an effective treatment regimen which combines virotherapy with immunotherapy. IFN-α and co-stimulator IL-2 along with tumor cell lysate vaccination with intratumoral administration of oncolytic vesicular stomatitis virus (VSV) resulted in regression of established TC1 papilloma tumor model in C57BL/6 mice. The remarkable results especially in the group receiving tumor vaccination and virotherapy together (TC1-VSV) were obtained...
2018: Acta Virologica
R I Tiwari, S S Bhullar, N H Chandak, N N Baheti, H F Daginawala, L R Singh, R S Kashyap
Viral infections of the central nervous system (CNS) occur sporadically and have been extensively studied because of the potential for permanent neurological damage or death. The neurotropic viruses have been reported to lead to various CNS infections. The objective of the present study is to develop an antigen detection ELISA protocol for detection and quantification of viral antigen in CNS infections by assessing the usefulness of antipeptide antibodies against potential peptides of cytomegalovirus (CMV), Epstein-Barr virus (EBV), varicella zoster virus (VZV), Japanese encephalitis virus (JEV), dengue (DENV), West Nile virus (WNV) and Chandipura virus (CHPV)...
2018: Acta Virologica
I Hamim, W Borth, M J Melzer, J Hu
Aphid-transmitted papaya ringspot virus (PRSV) is the greatest disease threat to the commercial production of papaya worldwide. Specific ultrasensitive assays are important for the early detection of PRSV in the field. We have developed a single-tube nested PCR (STNP) assay to address this need. Two nested PCR primer sets were designed to target the P3 gene of PRSV. The annealing temperatures and concentrations of both primer pairs were optimized to reduce potential competition between primer sets in STNP...
2018: Acta Virologica
M T Zabihipetroudi, A Ghalyanchilangeroudi, V Karimi, R Khaltabadifarahani, M Hashemzadeh
Infectious bronchitis virus (IBV), a major pathogen of the domestic fowl, exhibits extensive antigenic variation. IBV is a member of the Coronaviridae family and the genus Gammacoronavirus. A new infectious bronchitis virus serotype can emerge from only very few amino acid changes within the major peplomer glycoprotein, namely in its S1 part forming the virion spike. Principally, the serotypes are identified by virus neutralization (VN) tests. This study is aimed to investigate the neutralizing efficiency of H52, H120, and 4/91 antiserum against IBV genotypes (IS-1494, IS-720, 793/B, IR-1) recently circulating in Iran...
2018: Acta Virologica
A Ghosh, P A Alladi, G Narayanappa, R Vasanthapuram, A Desai
Chikungunya virus (CHIKV), a re-emerging Alphavirus, causes chronic myalgia and arthralgia in infected individuals. However, the exact pathophysiology remains undefined till date. Virus induced time course changes in host cells at the ultrastructural level and host cytoskeleton have been reported for other alphaviruses such as Sindbis and Semliki Forest virus. Few studies have tried to delineate the same for CHIKV leading to some understanding of the replication process. Selective CHIKV infection of progenitor cells involved in muscle repair has been proposed as a cause of myalgia; albeit the outcome of infection on these cells has not been reported...
2018: Acta Virologica
K-A Hwang, S Park, J H Ahn, J-H Nam
It has been previously reported that adenovirus 36 (Ad36) infection is associated with obesity in humans and other animals. However, there is no clinically available standard protocol to detect Ad36 DNA. In this study, we developed a method for quantitative and rapid detection of Ad36 DNA. Using a TaqMan probe quantitative polymerase chain reaction (qPCR), we identified that the E3 and E4orf1 regions specifically detect Ad36 DNA, because these regions did not show cross reactivity with other types of adenoviruses...
2018: Acta Virologica
Q Meng, M Qiao, S Gong, L Tian, Ch Li, J Qiao, D Meng, Y Wu, K Cai, Z Zhang, X Cai
Porcine bocavirus (PBoV) is a recently discovered, non-enveloped and single-stranded DNA virus that can infect pigs. In order to understand PBoV infection and its genetic characterization in piglets in Xinjiang China, PBoV was detected by PCR in 156 clinical samples from 1-month-old piglets. PBoV was detected in 9 clinical samples, with a prevalence rate of 5.77% (9/156). Then nonstructural protein NS1 gene was amplified, sequenced and used for phylogenetic analysis. The results showed that the prevalence rate in the sick piglets was 9...
2018: Acta Virologica
K Briestenská, M Janíková, P Kabát, D Csepányiová, J Zukal, J Pikula, V Kováčová, P Linhart, H Banďouchová, J Mistríková
No abstract text is available yet for this article.
2018: Acta Virologica
M Muhsen, P Lee
Homologous interference in vitro is defined as the ability of primary viral infection to prevent secondary homologous superinfection. Non-cytopathic bovine viral diarrhea virus (ncp BVDV) has been classified according to the exaltation of Newcastle disease phenomenon (END) as END positive (E+) and END negative (E-) strains. Simultaneous inoculation of MDBK-SY cell monolayers with BVDV/E- virus and a three log higher amount of BVDV RK13/E+ virus, leads to acquisition of the BVDV/E- feature of blocking Newcastle disease virus (NDV) infection in cells...
2018: Acta Virologica
X Luo, Y Liu, R Jia, H Shen, X Wang, M Wang, D Zhu, S Chen, M Liu, X Zhao, Y Wu, Q Yang, Z Yin, A Cheng
Duck Tembusu virus (DTMUV) is a newly emerging enveloped flavivirus. This study shows the ultrastructure of DTMUV using viral purification, negative staining and electron microscopy. Electron microscopic examinations revealed mature DTMUV particles with 50 to 75 nm in diameter and typical enveloped flavivirus structure that consists of the internal nucleocapsid, an inner layer of lipid bilayer and an external layer of E glycoprotein ectodomain. Particles appear to be mostly spherical. In particular, RNA core is deep colored and dense, both capsid and lipid bilayer are clearly visible, the capsid forms regular hexagon, and E glycoprotein ectodomain forms a fringe instead of visible spikes...
2018: Acta Virologica
W K Reeves, M M Miller, W E Gruner
The U.S. Air Force conducts vector and arbovirus surveillance worldwide. We report on two Rhabdoviridae detected at Holloman Air Force Base in Otero County, New Mexico including the nearly complete 11-kb genome of Merida virus, which was not previously known from the USA, and a novel virus tentatively named Dillard's Draw virus. Merida virus was previously only known from Mexico. Dillard's Draw virus represents a novel arbovirus most closely related to an avian pathogen from Australia and shares some genetic similarity to Durham virus from the eastern United States...
2018: Acta Virologica
S Ampuero, R Andaur, M Milano, M Moreno, L Lizama, C Larrañaga, U Urzúa
Respiratory syncytial virus (RSV) is the major cause of acute lower respiratory tract infection in infants. Winter outbreaks in Chile result in 5% of infected children hospitalized, with 0.01% mortality. Increased evidence indicates that viral and host factors modulate the severity of infection. Using DNA microarrays, we characterized the genome-wide transcriptional response of lung mucoepidermoid cells (NCI-H292) at 0, 24, 48, 72 and 96 hours post-infection (hpi) with a single dose of RSV/A. During the whole studied period, a bi-phasic gene expression profile was observed by a total of 330 differentially expressed genes...
2018: Acta Virologica
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