Abril Alondra Barrientos-Bonilla, Paola Belem Pensado-Guevara, Abraham Puga-Olguín, Rasajna Nadella, Aurora Del Carmen Sánchez-García, Laura Mireya Zavala-Flores, Arnulfo Villanueva-Olivo, Iliana Tamara Cibrián-Llanderal, María de Jesús Rovirosa-Hernández, Daniel Hernandez-Baltazar
Bromodeoxyuridine (BrdU) is used in studies related to cell proliferation and neurogenesis. The multiple intraperitoneal injections of this molecule could favor liver function profile changes. In this study, we evaluate the systemic and hepatocellular impact of BrdU in male adult Wistar rats in 30 %-partial hepatectomy (PHx) model. The rats received BrdU 50 mg/Kg by intraperitoneal injection at 0.5, 1, 2, 3, 6, 9 and 16 days after 30 %-PH. The rats were distributed into four groups as follows, control, sham, PHx/BrdU(-) and PHx/BrdU(+)...
November 27, 2023: Acta Histochemica
Ruzanna Shushanyan, Anna Grigoryan, Tamara Abgaryan, Anna Karapetyan
High altitude sickness is a life-threatening disease that occurs among acclimatized individuals working or living at a high altitude accompanied by hypobaric hypoxia exposure. The prolonged influence of hypobaric hypoxia on the brain may trigger neuronal damage and cell death due to an oxygen deficiency. The purpose of the current study was to investigate the histomorphological changes in the hippocampus, cerebral cortex, cerebellar cortex, and striatum of the rat's brain following chronic hypobaric hypoxia...
November 17, 2023: Acta Histochemica
Anthea Miller, Giuseppina Cutroneo, Giorgia Pia Lombardo, Roberta D'Angelo, Socrate Pallio, Alba Migliorato, Angelo Fumia, Angelo Favaloro, Eugenia Rita Lauriano, Simona Pergolizzi
Crohn's disease (CD) and ulcerative colitis (UC) are both inflammatory bowel diseases (IBD). Unlike UC, which is limited to the mucosa of the colon, CD inflammation is characterized by chronic mucosal ulcerations affecting the entire gastrointestinal tract. Goblet cells (GCs) can be found in some lining epithelia, particularly in the respiratory and digestive tracts. GCs represent the main source of mucin that are the significant components of the mucus layer; hypertrophy of GCs and an increase in mucin production are observed in many enteric infections...
November 16, 2023: Acta Histochemica
Laís Bitencourt Guimarães, Daniel Portela Dias Machado, Beatriz Ferreira Carvalho Versiani Caldeira, Larissa Tiemi Matuzake Vieira, Gabriela Alves Santos, Fabiana Rocha Araújo, Leonardo Teotônio Machado, Dawidson Assis Gomes, Natália de Melo Ocarino, Rogéria Serakides, Amanda Maria Sena Reis
Kisspeptin (Kp-10) is a neuropeptide that binds to GPR54 receptors, exerting several functions mainly in the nervous and reproductive systems of the body. However, its effects and mechanisms of action on the skeletal system remain poorly understood. This study evaluated the effects of different concentrations of Kp-10 on in vitro osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs) extracted from the bone marrow (BM) of adult Wistar rats. Two-month-old female rats were euthanized to extract BM from long bones to obtain MSCs...
November 8, 2023: Acta Histochemica
Lorenzo Alibardi
PURPOSE: Lizard regeneration derives from the re-activation of a number of developmental genes after tail amputation. Among genes with the highest expression, as indicated from the transcriptome, is lix1 which functional role is not known. METHOD: An antibody that cross-reacts with the lizard Podarcis muralis lix1 has been utilized to detect by immunofluorescence the sites of localization of the protein in the regenerating tail. RESULTS: Lix1-protein is almost exclusively localized in the regenerating spinal cord (ependyma) and nerves growing into the blastema, in sparse blastema cells but is undetectable in other tissues...
November 8, 2023: Acta Histochemica
XinYi Chen, XiaoXiao Zheng, Ting Shen, Ting He, YangQi Zhao, Yi Dong
UV-induced corneal damage is a common ocular surface injury that usually leads to corneal lesions causing persistent inflammation. High mobility group box 1 (HMGB1) is identified as an inflammatory alarm in various tissue injuries. Here, this study first evaluates the repair effect of the HMGB1-selective inhibitor GLY in UV-induced corneal damage; Secondly, the inhibitory effect of GLY on UV-induced corneal damage induced inflammation and the potential therapeutic mechanism of GLY were studied. GLY effectively attenuates the expression of UV-induced inflammatory factors and HMGB1, TLR/MyD88, NF-κB signaling pathway genes at the mRNA and protein levels...
November 6, 2023: Acta Histochemica
Qianqian Guo, Shichao Han
Pterygium is a common eye surface disease with high recurrence and unclear pathogenesis. In current study, RNA sequencing was conducted in 6 pairs of human pterygium and conjunctival tissues, and Matr3 as a novel candidate gene was significantly reduced in pterygium compared to control tissues. Moreover, immunoprecipitation was performed to pull down MATR3, and WTAP specially interacting with MATR3 in control but not pterygium was identified by mass spectrum. Immunoprecipitation was performed to validate the interaction between MATR3 and WTAP/METTL3/METTL14 complex...
October 30, 2023: Acta Histochemica
Yan Wu, Jun Zhang, Xintian Wang, Yuncong Xu, Jinxu Zheng
OBJECTIVE: Idiopathic pulmonary fibrosis (IPF) is considered as a chronic interstitial lung disease with underlying mechanism of IPF remaining unclear, while there are no definitive treatment options. In recent years, scientists have gradually paid attention to the influence of angiogenesis on IPF. Because IPF is a progressive with microvascular remodeling disorder, scientists have postulated that angiogenesis may also be one of the initiating and contributing factors of the disease. Bupleurum is a common natural Chinese herbal medicine with antibacterial, anti-inflammatory, anti-tumor and other pharmacological effects...
October 12, 2023: Acta Histochemica
Xiaojiao Liu, Shunqiao Jin, Jiao Liu, Xuezhu Xu
BACKGROUND: Adipose mesenchymal stem cell (AMSC)-derived exosomes are promising novel factors for wound repair and regeneration. This study aimed to explore the potential roles and underlying mechanisms of specific miRNA in wound healing using AMSC-derived exosomes as carriers. METHODS: The expression profiles of GSE197840 were downloaded to screen for differentially expressed miRNAs (DEmiRNAs), and the corresponding genes of the identified miRNAs were predicted...
October 12, 2023: Acta Histochemica
Juan F Santibanez, Cesar Echeverria, Carola Millan, Felipe Simon
The ability to differentiate into cells of different lineages, such as bone cells, is the principal value of adult mesenchymal stem cells (MSCs), which can be used with the final aim of regenerating damaged tissue. Due to its potential use and importance in regenerative medicine and tissue engineering, several questions have been raised regarding the molecular mechanisms of MSC differentiation. As one of the crucial mediators in organism development, the transforming growth factor-beta (TGF-β) superfamily directs MSCs' commitment to selecting differentiation pathways...
October 7, 2023: Acta Histochemica
Xiao-Jiao Wei, Yue-Lin Huang, Tian-Quan Chen, Xiao-Jun Yang
PURPOSE: Telocytes (TCs), a novel type of stromal cells found in tissues, induce macrophage differentiation into classically activated macrophages (M1) types and enhance their phagocytic function. The purpose of this study was to investigate the inhibitory effects of TC-induced M1 macrophages on endometriosis (EMs). METHODS: mouse uterine primary TCs and endometrial stromal cells (ESCs) were isolated and identified using double immunofluorescence staining. For the in vitro study, ESCs were treated with TC-induced M1 macrophages, and the vascular endothelial growth factor (VEGF), matrix metalloproteinase 9 (MMP9), and nuclear factor kappa B (NF-κb) genes were identified by quantitative real-time PCR (qRT-PCR) or western blotting (WB)...
October 7, 2023: Acta Histochemica
Dingwu Li, Chenhui Ye, Peihao Liu, Ting Sun, Yunsheng Qin, Xingyong Wan
OBJECTIVES: Cholestatic liver diseases are characterized by hepatocellular damage, cholangiocyte proliferation, and progressive fibrosis. Bile duct ligation (BDL) is widely used to resemble liver injuries induced by cholestasis. Peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC1α) was reported to play a critical role in multiple biological responses. Nevertheless, whether PGC1α is involved in bile acid metabolism and biliary disorders remains unclear...
October 7, 2023: Acta Histochemica
Gabriella Meier Bürgisser, Dorothea M Heuberger, Pietro Giovanoli, Maurizio Calcagni, Johanna Buschmann
Situated in the oral cavity, the rabbit palatine tonsils are part of the mucosal immune system and help to defend the body against foreign pathogens. Expressed as two oval protrusions in the wall of the oropharynx, the rabbit palatine tonsils are characterized by excretory ducts and trabeculae. We here compare paraffin embedded and cryosections of the healthy rabbit tonsils. This analysis centers on evaluating the differential outcomes resulting from the application of these fixation methodologies in conjunction with immunohistochemical assays targeting collagen I, collagen III, fibronectin, α-smooth muscle actin (α-SMA), and ki67...
October 5, 2023: Acta Histochemica
Kyeong-Min Kim, Hyo-Eun Son, Young-Ju Lim, Won-Gu Jang
Topiramate [2,3:4,5-bis-o-(1-methylethylidene) β-D-fructo-pyranose sulfamate; TPM] is one of the most used new-generation antiepileptic drugs. It has been reported to regulate the differentiation of human bone cells. However, the molecular mechanism of TPM in osteoblast differentiation is not fully elucidated. In the present study, we examined the effect of TPM on osteogenic differentiation of C3H10T1/2, MC3T3-E1, primary mouse calvarial cells, and primary bone marrow stem cells (BMSCs). Primary cells were isolated from mice calvaria and bone marrow respectively...
September 25, 2023: Acta Histochemica
Irene Rosa, Patrizia Nardini, Bianca Saveria Fioretto, Daniele Guasti, Eloisa Romano, Eleonora Sgambati, Mirca Marini, Mirko Manetti
Since their relatively recent discovery, telocytes (TCs) have been described as peculiar cells strategically positioned in the stromal tissue component of multiple organ systems of the mammalian body including female reproductive organs (i.e., ovary, uterine tube, and uterus). Nevertheless, current knowledge of TCs in the vagina is very limited. The present study was therefore undertaken to investigate the existence and characteristics of TCs in the stromal tissue of human vaginal mucosa by means of immunohistochemistry, immunofluorescence confocal microscopy, and transmission electron microscopy...
September 25, 2023: Acta Histochemica
Zixiu Chen, Junhong Zhao, Cangyu Wang, Xiang Liu, Zihua Chen, Jianda Zhou, Lei Zhang, Cuiping Zhang, Haihong Li
BACKGROUND: Each eccrine sweat gland (ESG) is a single-tubular structure with a central lumen, and the formation of hollow lumen in the initial solid cell mass is a key developmental process. To date, there are no reports on the mechanism of native ESG lumen formation. METHODS: To investigate the lumen morphogenesis and the lumen formation mechanisms of Sprague-Dawley (SD) rat ESGs, SD rat hind-footpads at E20.5, P1-P5, P7, P9, P12, P21, P28 and P56 were obtained...
September 25, 2023: Acta Histochemica
Diana I Romero-Sánchez, Sonia Vázquez-Santana, Rafael A Alonso-Alvarez, Jorge M Vázquez-Ramos, Aurora Lara-Núñez
In maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase controlling proliferation (a negative signal that slows down the cell cycle)...
September 15, 2023: Acta Histochemica
Caio César da Silva Barros, Luiz Miguel da Rocha Santos, Mara Luana Batista Severo, Márcia Cristina da Costa Miguel, Cristiane Helena Squarize, Éricka Janine Dantas da Silveira
Central giant cell granuloma (CGCG) is a benign jaw lesion with variable clinical behavior. Cell cannibalism is a cellular process associated with aggressiveness and invasion in malignant neoplasms. Here, we morphologically investigated cell cannibalism as an auxiliary method to predict CGCG clinical behavior. Cell cannibalism was quantitatively evaluated in 19 cases of peripheral giant cell granuloma (PGCG), 38 cases of CGCG (non-aggressive and aggressive), and 19 cases of giant cell tumor of bone (GCT) stained with hematoxylin and eosin...
August 30, 2023: Acta Histochemica
Qiyan Deng, Lumei Liu, Ran Tang, Dehai Xian, Jianqiao Zhong
BACKGROUND: Fibroblasts (FBs) have been widely used as a typical in vitro cell model for investigating the biological processes and cell pathophysiological mechanisms. However, FBs are prone to senescence in cell culture process after several passages. Thus, a new approach to cell culture is quite required to enhance the viability of cells. OBJECTIVE: To explore a novel method of cell culture based on skin FBs. METHODS: Dermal tissue blocks were obtained from BALB/c neonatal mice and randomly divided into experimental group and control group...
August 26, 2023: Acta Histochemica
Martha Lilia Tena-Suck, Ma Elena Hernández-Campos, Alma Ortiz-Plata, Citlaltepetl Salinas-Lara, Ana Laura Colín-González, Abel Santamaría
No abstract text is available yet for this article.
August 10, 2023: Acta Histochemica
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