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Acta Histochemica

Mehmet Özbek, Alev Gürol Bayraktaroğlu
Peyer's patches are known as the immune sensors of the intestine because of their ability to transport luminal antigens. The objective of this study was both to assess the prenatal and postnatal development of sheep ileal Peyer's patches with respect to histomorphology, distribution of CD4+ and CD8+ cells, and localization of proliferating and apoptotic cells, and to examine the morphology of M cells and expression of CK18 in follicle associated epithelium (FAE). We also hypothesized that CK18 could be a potential marker for M cell...
February 8, 2019: Acta Histochemica
Katarzyna Czarzasta, Lukasz Koperski, Agnieszka Segiet, Maciej Janiszewski, Marek Kuch, Barbara Gornicka, Agnieszka Cudnoch-Jedrzejewska
It is well known that obesity contributes to the development of systemic inflammatory responses, which in turn may be involved in the process of interstitial fibrosis and left ventricular (LV) remodelling. Activation of pro-inflammatory factors such as transforming growth factor β (TGF-β) can directly stimulate mitogen-activated protein kinase (MAPK) p38 and JNK. The aim of the study was to evaluate the level of TGF-β and MAPK p38 and JNK in the LV in Sprague Dawley (SPRD) rats maintained on a high fat diet (HFD)...
February 4, 2019: Acta Histochemica
Hui Shen, Xiaorui Fan, Zhen Zhang, Huaming Xi, Rui Ji, Yihui Liu, Meishan Yue, Qinghong Li, Junping He
Heat stress damaged spermatogenesis and semen quality, however, the exact molecular mechanism is not clear. The objective of this study was to determine the effects of elevated ambient temperature and local testicular heating on the expressions of heat shock protein 70 and androgen receptor in boar testes. A growing body of evidence demonstrated that germ cell apoptosis can be aggravated by heat stress and androgen deprivation, and at normal temperature, withdrawal of androgen led to germ cell apoptosis. There were no reports that heat stress damaged spermatogenesis has relationship with androgen...
February 2, 2019: Acta Histochemica
Erhan Sahin, Ezgi Bektur, Dilek Burukoglu Donmez, Cengiz Baycu, Ozgür Devrim Can, Varol Sahinturk
AIM: The aim of this study was to investigate the effects of mirtazapine, which is anti-oxidative and antidepressant agent, on the kidney damage caused by diabetes mellitus. MATERIALS AND METHODS: The rats were randomly divided into three groups (n = 7 animals in each group). The group I rats served as control and they received 0.1 mol/L of citric acid buffer (pH = 4.5) as vehicle. The rats in the group II (DM group) and III (DM + Mirtazapine-treated group) were treated intraperitoneally with a single dose of 55 mg/kg streptozotocin dissolved in 0...
January 30, 2019: Acta Histochemica
Gabriela Beatriz Olea, Esteban Omar Cheij, Lucila Marilen Curi, Ana Paula Cuzziol Boccioni, Jorge Abel Céspedez, Daniel Marcelo Lombardo
A detailed description of the tegument and parotoid glands of pre-metamorphic, post-metamorphic, juvenile and adult individuals of Rhinella bergi is presented to provide an exhaustive analysis of the integumentary characteristics of this species. Fragments of the tegument were fixed in Bouin solution and preserved in buffered Formol 10%. Subsequently, scanning electron microscopy (SEM) was performed to characterize the macroscopic structure of these regions. Microscopic observations were made from histological sections stained with Hematoxylin and Eosin, Alcian Blue (pH 2,5), PAS-H, Coomassie Blue, Oil Red, and Bielschowsky Impregnation...
January 21, 2019: Acta Histochemica
Wei Cheng, Shutao Zheng, Li Li, Qin Zhou, Haipeng Zhu, Jun Hu, Hongbin Luo
Chloride channel 3 (CIC-3) has been suggested to be implicated in the carcinogenesis though; it still remains ill understood in hepatocarcinoma, especially in terms of clinicopathological meaning of its expression. Given this, herein, to understand the clinicopathological significance of CIC-3 expression in hepatocarcinoma, Immunohistochemistry was performed to examine the level of CIC-3, followed by statistical analysis of the correlation between expression versus clinicopathological variables, including gender, age, TNM classifications, tumor size, lymph node metastasis and overall prognosis...
January 21, 2019: Acta Histochemica
Roza Benabdesselam, Alvaro Rendon, Latifa Dorbani-Mamine, Hélène Hardin-Pouzet
Dp71 is the major form of dystrophins (Dp) in the supraoptic nucleus (SON) and in the neural lobe of hypophysis (NL/HP). Dp71-null mice exhibit a hypo-osmolar status attributed to an altered osmosensitivity of the SON and to a perturbed vasopressinergic axis. Because oxytocin (OT) is implicated in osmoregulation via natriuresis, this study explored the oxytocinergic axis in Dp71-null mice after salt-loading (SL). Under normosmolar conditions, OT-mRNA expression was higher in the Dp71-null SON compared to wild-type (wt) and the OT peptide level has not changed...
January 11, 2019: Acta Histochemica
Phornphan Phrompanya, Kanokporn Saenphet, Supap Saenphet
Because of the wide variety of cells which are arrayed in the tissue of the gastrointestinal tracts of these two fish, basic histological examination is not appropriate to clarify the histological structure. But histochemical techniques can be applied to identify the chemical differences contained in the tissues. The present study aimed to compare the histochemical characteristics of the gastrointestinal tracts of adult fishes which consume different types of food: the Nile tilapia is herbivore and the hybrid catfish is carnivore...
January 10, 2019: Acta Histochemica
Grzegorz Panasiewicz, Aleksandra Lipka, Marta Majewska, Martyna Bieniek-Kobuszewska, Alexander P Saveljev, Bozena Szafranska
Pregnancy-associated glycoproteins (PAGs) are abundant embryo-originated products expressed in the pre-placental trophoblast and, later, in the post-implantational chorionic epithelium of some mammalian species. This paper describes the identification and cellular immunolocalization of the chorionic PAG family in the discoidal-type placenta of the brown bear (Ursus arctos L. - Ua), in which the PAGs were named 'UaPAG-Ls'. The study used: 1) Western blot for total placental glycoproteins; and 2) cross-species heterologous double fluorescent immunohistochemistry (IHC) for cellular immune-localization of the PAGs...
January 4, 2019: Acta Histochemica
Masafumi Amano, Noriko Amiya, Takehiko Yokoyama
The gonadotropin-releasing hormone (GnRH) gene sequence has been identified in an annelid polychaete marine worm using continual genome sequencing. The distribution of GnRH immunoreactive (ir) cell bodies and fibers in the nerve ganglion of the clam worm Perinereis aibuhitensis (Polychaeta) was examined by immunohistochemistry using a newly produced rabbit polyclonal antibody raised against the marine worm GnRH (mwGnRH). The specificity of the antibody was confirmed by dot blot assay. The antibody cross-reacted with mwGnRH, but not with other forms of GnRH such as octopus GnRH, tunicate GnRH-I, II, owl limpet GnRH, and lamprey GnRH-II...
January 4, 2019: Acta Histochemica
Tao Wang, Ruiqiao Yan, Xiaoyuan Xu, Xingnuan Li, Lingling Cao, Liyun Gao, Jianyun Liu, Xiaoou Zhou, Hui Yu, Xinping Wang, He Jiang, Yaofang Yang
Understanding the mechanisms of adipogenic differentiation may lead to the discovery of novel therapeutic targets for obesity. The natural plant polyphenol compound curcumin can improve obesity-associated inflammation and diabetes in obese mice. The role of curcumin in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) is still unclear. We used hMSCs to investigate the details of the mechanism underlying the adipogenic effects of curcumin. At different time points (i.e., 5 days and 10 days) of hMSC adipocyte differentiation, an accumulation of large lipid droplets was analyzed in Oil Red O-stained cultured cells in two curcumin (5 μM and 10 μM) groups and the control group...
January 3, 2019: Acta Histochemica
Sirinart Chomean, Manop Nantabut, Watcharapong Kongtia, Kolunya Saenguthai, Chollanot Kaset
Dye extracts from plants have been valuable not only for the economy but also environmental sustainability. There have been many reports on the utilization of natural dyes extracted from various sources for staining of biological tissues. This study aimed to investigate the extraction of natural dye from black rice (Oryza Sativa), butterfly pea (Clitoria ternatea), fresh roselle (Hibiscus sabdariffa), and mulberry (Morus alba) to stain of human spermatozoa for morphology assessment. The results showed that black rice extracted from solvents C containing 5 ml of absolute ethanol, 10 g of potassium alum and 100 ml of distilled water is the best dye for human spermatozoa evaluation comparable to the rapid PAP and Dip quick® stain...
December 31, 2018: Acta Histochemica
Sabrina Souttou, Roza Benabdesselam, Lourdes Siqueiros-Marquez, Madina Sifi, Maha Deliba, Ophelie Vacca, Hugo Charles-Messance, Cyrille Vaillend, Rendon Alvaro, Xavier Guillonneau, Latifa Dorbani-Mamine
Dystrophins (Dps) are the sub-membranous proteins that work via the dystrophin-associated proteins complex, which comprises β-dystroglycan (β-DG), a cell surface receptor for extracellular matrix. Recently, we have revealed β-DG decrease and central function impairment of supraoptic nucleus (SON) in Dp71 deficient adult mice, opening the question on the profiles of Dps and β-DG during SON development. At birth and the age of 10, 20 and 60 days, we examined the expression by RT-PCR and Western-blotting, and the distribution by immunohistochemistry of Dps and β-DG...
December 28, 2018: Acta Histochemica
Maha Abu Gazia, Mohammed Abu El-Magd
The increasing application of carbon nanotubes (CNTs) within environmental, occupational and consumer settings has raised concerns regarding their biosafety and adverse effects on human health. The present study was designed to investigate the possible adverse effect of pristine and functionalized (amylated and polyethelene glycol coated) multi-walled (MW) CNTs on rat kidney with special concern to the histological alterations and the associated oxidative stress, apoptosis and inflammation. Healthy male albino rats (n = 40) were randomly divided into 4 groups: group I (control), group II (pristine MWCNTs), group III (amylated MWCNTs) and group IV [polyethelene glycol (PEG)-coated MWCNTs]...
December 24, 2018: Acta Histochemica
Anna T Grazul-Bilska, Casie S Bass, Samantha L Kaminski, Kaitlyn K Ebel, Elizabeth Leke, Jiratti Thammasiri, Aree Kraisoon, Chainarong Navanukraw, Marisa Holst, Mckenzi Shelton, Sheri T Dorsam, Dale A Redmer
The aim of this study was to investigate the role of the nitric oxide (NO) system in ovarian function, by determining if arginine (Arg) supplementation impacts follicle number, cell proliferation, and expression of the NO system members in nutritionally compromised ewes. Ewes were randomly assigned into maintenance (C, 100% requirements), excess (O; 2xC), or restricted (U; 0.6xC) diets 8 weeks prior to Arg treatment. Ewes were individually fed twice daily with pelleted diets. Ewes from each nutritional group were randomly assigned to one of two treatments: saline or Arg, which was initiated on day 0 of the estrous cycle and administered 3 times per day...
December 24, 2018: Acta Histochemica
Mohamed I Abdrabou, Ebtihal M M Elleithy, Noha A E Yasin, Youssef M Shaheen, Mona Galal
The present study was conducted to evaluate the ameliorative effects of Allium sativum (garlic) as well as Spirulina maxima on lead acetate toxicity in rat testes. Forty adult, male, albino rats were divided into four groups (10 rats/each): group I served as the control; group II contained rats that received lead acetate (100 mg/kg); group III contained rats that received both lead acetate (100 mg/kg) and garlic (600 mg/kg); and group IV contained rats that received both lead acetate (100 mg/kg) and spirulina (500 mg/kg)...
December 24, 2018: Acta Histochemica
Lalrawngbawli Annie, Guruswami Gurusubramanian, Vikas Kumar Roy
Dexamethasone, a synthetic glucocorticoid has been used as an immunosuppressive and anti-inflammatory and affects reproduction. It has been suggested that testicular steroidogenesis involves PGC-1α and visfatin as key regulators. Previous studies have shown that dexamethasone down-regulates PGC-1α and visfatin expression in muscle and mammary epithelial cells respectively. However, the effect of dexamethasone on testicular visfatin and PGC-1α expressions has not been investigated. The aims of the present study were to investigate the effect of dexamethasone, on the expression of PGC-1α, visfatin and antioxidant enzymes activities in mouse testis...
December 19, 2018: Acta Histochemica
Lahcen Tamegart, Abdellatif Abbaoui, Rachida Makbal, Mohamed Zroudi, Brahim Bouizgarne, My Mustapha Bouyatas, Halima Gamrani
Lead (Pb) is a metal element released into the atmosphere and a major source of environmental contamination. The accumulation and concentration of this metal in a food web may lead to the intoxication of the body, more precisely, the nervous system (NS). In addition, Pb-exposure can cause structural and functional disruption of the NS. Studies have shown that Pb-exposure could be a risk factor in the development of Parkinson's disease (PD). The latter is related to dopaminergic deficiency that may be triggered by genetic and environmental factors such as Pb intoxication...
December 17, 2018: Acta Histochemica
Fumihiko Sato, Shun-Ichi Chitose, Kiminori Sato, Takashi Kurita, Kiminobu Sato, Hirohito Umeno, Hirohisa Yano
The latest research suggests cells in the maculae flavae located at both ends of the lamina propria of the human vocal fold mucosa have stemness. This study investigated the differentiation potential of the cells in the maculae flavae of the human vocal fold mucosa. Four normal human adult vocal folds from surgical specimens were used. After extraction of the anterior maculae flavae located at the anterior end of the lamina propria of the human vocal fold mucosa under microscope, the maculae flavae were minced, cultured and proliferated in mesenchymal stem cell growth medium and morphological features were assessed...
December 15, 2018: Acta Histochemica
Thanapong Kruangkum, Jirawat Saetan, Charoonroj Chotwiwatthanakun, Rapeepun Vanichviriyakit, Scott F Cummins, Chaitip Wanichanon, Prasert Sobhon
The giant freshwater prawn, Macrobrachium rosenbergii, is an important aquaculture species. A better understanding of the molecular components of reproduction in this species would help to advance the prawn production. In the present study, we demonstrated the presence of an egg laying hormone (ELH)-like peptide in the male reproductive system. First, an antibody to the abalone (a)ELH was generated, and by Western blot it was shown to specifically bound to a protein from the male M. rosenbergii reproductive tissues with a similar size to molluscan ELH...
December 14, 2018: Acta Histochemica
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