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Annals of Clinical Biochemistry

Matthew Hand, Andrea Crampton, Annette Thomas, Eric Stephen Kilpatrick
BACKGROUND: Clinical laboratory instrument verification testing is often an accreditation requirement. However, it is not known what verification procedures are in routine use or how often the process identifies problems which need addressing prior to testing clinical samples. OBJECTIVE: To investigate which standards are currently being used for laboratory verification in UK and New Zealand (NZ) clinical laboratories, and to help establish if the activity justifies the effort required...
January 23, 2019: Annals of Clinical Biochemistry
Shipeng Li, Jianling Sun, Wenchao Hu, Yan Liu, Dan Lin, Haiping Duan, Fengting Liu
OBJECTIVE: Adropin, a newly identified regulatory protein encoded by Enho gene, is correlated with insulin sensitivity and diabetes. The aim of this study is to determine whether serum and vitreous adropin concentrations are correlated with the presence of diabetic retinopathy (DR). METHODS: A population of 165 patients with type 2 diabetes mellitus (T2DM) [52 without DR, 69 with non-proliferative diabetic retinopathy (NPDR), and 44 patients with proliferative diabetic retinopathy (PDR)] was enrolled in this study...
December 4, 2018: Annals of Clinical Biochemistry
Ramona Dolscheid-Pommerich, Alexander Kreuzer, Ingo Graeff, Birgit Stoffel-Wagner, Andreas Mueller, Florian Kipfmueller
Background Extracorporeal membrane oxygenation procedure (ECMO) has been established in the therapy of respiratory insufficient infants with congenital diaphragmatic hernia (CDH). In CDH newborns, a delay in transfer to an ECMO center is associated with a sharp increase in mortality. Predictive factors for ECMO support are urgently needed. We evaluated routine parameters of the first blood withdrawal after birth in CDH infants, hypothesizing that early signs in bone marrow affecting hematology parameters for early regulation of potentially post birth hypoxia are predictive factors for ECMO support...
December 4, 2018: Annals of Clinical Biochemistry
Takuya Shimura, Makoto Kurano, Yoshifumi Morita, Naoyuki Yoshikawa, Masako Nishikawa, Koji Igarashi, Satoshi Shimamoto, Junken Aoki, Yutaka Yatomi
BACKGROUND: Invasion of the central nervous system (CNS) by hematological malignancies is diagnosed by cytological analyses of cerebrospinal fluid (CSF) or diagnostic imaging, while quantitative biomarkers for CNS invasion are not available and needed to be developed. Methods; In this study, we measured the levels of autotaxin (ATX) and soluble IL-2 receptor (sIL-2R) in cerebrospinal fluid (CSF) and evaluated their usefulness as biomarkers for CNS invasion. RESULTS: We observed that both the ATX and sIL-2R levels in CSF were higher in subjects with CNS invasion than in those without, and the CSF levels were independent from the serum levels of these biomarkers...
December 4, 2018: Annals of Clinical Biochemistry
Jianjun Xu, Yun Xia, Suzhen Li, Xiang Cheng, Shaobo Hu, Xing Zhou, Yang Gao, Guoliang Wang, Qichang Zheng
BACKGROUND: The aspartate aminotransferase (AST) / alanine aminotransferase (ALT) ratio(AST/ALT) is an independent predictor of hepatic disease. OBJECTIVES: To evaluate the association between pre-operative AST/ALT and post-operative acute kidney injury(AKI) in hepatocellular carcinoma (HCC) patients. METHODS: A total of 422 hepatitis B- or C- virus associated HCC patients, who underwent hepatectomy between September 2012 and April 2018, were enrolled this retrospective study...
November 19, 2018: Annals of Clinical Biochemistry
Emma Rose McGlone, Stephen R Bloom
Bile acids have important roles in the regulation of lipid, glucose and energy metabolism. Metabolic diseases linked to obesity, including type 2 diabetes mellitus and non-alcoholic fatty liver disease, are associated with dysregulation of bile acid homeostasis. Here basic chemistry and regulation of bile acids will be reviewed, as well as their metabolic effects. Changes in circulating bile acids associated with obesity and related diseases will be reviewed. Finally, pharmaceutical manipulation of bile acid homeostasis as therapy for metabolic diseases will be outlined...
November 19, 2018: Annals of Clinical Biochemistry
Saliha Mohammed Ismail Haji, Allison Chipchase, W Fraser, Javier Gomez
BACKGROUND: Alkaline phosphatase (ALP) isoenzyme analysis is an expensive and time-consuming laboratory test. We evaluated the effect of a locally-derived screening algorithm for ALP isoenzyme requests on the number of ALP isoenzyme analyses performed, laboratory cost, and patient care. METHOD: 110 ALP isoenzyme analysis requests from the year 2015 were reviewed and subsequent ALP concentrations were monitored over a 2-year period, to determine if the decision of performing/not performing ALP isoenzyme analysis, based on the algorithm, had an impact on patient care and laboratory cost...
November 17, 2018: Annals of Clinical Biochemistry
Anne Sophie Bargnoux, Vincent Azoury, Stephanie Badiou, Kada Klouche, Maëlle Plawecki, Nils Kuster, Anne Marie Dupuy, Jean-Paul Cristol
BACKGROUND: The study was designed to evaluate the analytical performances of two ERM-DA471/IFCC traceable cystatin C (CysC) reagents available on the market for urinary CysC (u-CysC) quantification. In addition, clinical relevance was assessed by measuring u-CysC in healthy controls and in patients with tubular dysfunction. METHODS: CysC in urine was measured by a particle enhanced nephelometric immunoassay (PENIA) using Siemens reagents and by a particle enhanced turbidimetric immunoassay (PENIA) using DiaSys reagents...
November 14, 2018: Annals of Clinical Biochemistry
G Woodward, R Pryke, B Hoppe, G Rumsby
BACKGROUND: The primary hyperoxalurias are inherited disorders of glyoxylate metabolism that lead to overproduction of oxalate, urolithiasis and renal failure. Delays in diagnosis can be costly in terms of preserving renal function. Here we present a rapid liquid chromatography tandem mass-spectrometry screening method for the analysis of metabolites (primary hyperoxaluria metabolites) produced in excess by primary hyperoxaluria patients that include glycolate, glycerate and 2,4-dihydroxyglutarate...
November 14, 2018: Annals of Clinical Biochemistry
Michael Jw Thompson, Venkat Parameswaran, John R Burgess
BACKGROUND: Patients with mutations of succinate dehydrogenase B (SDHB) and succinate dehydrogenase D (SDHD) are at high risk of paraganglioma necessitating surveillance. Chromogranin A has been proposed as a biochemical marker of paraganglioma. We sought to determine the diagnostic utility of chromogranin A in a population-based SDHx sample. METHODS: Tasmania is an island state with one tertiary referral centre for endocrine neoplasia. We performed a cross-sectional analysis of all adult SDHB ( n = 52) and SDHD ( n = 10) patients undergoing paraganglioma surveillance between 2011 and 2017...
November 14, 2018: Annals of Clinical Biochemistry
Soheil Madadi, Meysam Soleimani
No abstract text is available yet for this article.
November 8, 2018: Annals of Clinical Biochemistry
H A Kingma, F H van der Sluijs, M R Heiner-Fokkema
Background Congenital disorders of glycosylation (CDG) are a growing group of rare genetic disorders. The most frequently used screening method is sialotransferrin profiling using isoelectric focusing (IEF). Capillary zone electrophoresis (CZE) may be a simple and fast alternative. We investigated the Capillarys™ CDT assay (Sebia, France) to screen for N-glycosylation disorders, using IEF as gold standard. Methods Intra- and inter-assay precision were established, and analyses in heparin-anticoagulated plasma and serum were compared...
November 2018: Annals of Clinical Biochemistry
Paul Collinson
No abstract text is available yet for this article.
November 2018: Annals of Clinical Biochemistry
Michelle Ma Kip, Annemarie Schop, Karlijn Stouten, Soraya Dekker, Geert-Jan Dinant, Hendrik Koffijberg, Patrick Je Bindels, Maarten J IJzerman, Mark-David Levin, Ron Kusters
Background Establishing the underlying cause of anaemia in general practice is a diagnostic challenge. Currently, general practitioners individually determine which laboratory tests to request (routine work-up) in order to diagnose the underlying cause. However, an extensive work-up (consisting of 14 tests) increases the proportion of patients correctly diagnosed. This study investigates the cost-effectiveness of this extensive work-up. Methods A decision-analytic model was developed, incorporating all societal costs from the moment a patient presents to a general practitioner with symptoms suggestive of anaemia (aged ≥ 50 years), until the patient was (correctly) diagnosed and treated in primary care, or referred to (and diagnosed in) secondary care...
November 2018: Annals of Clinical Biochemistry
James John Logie, Christopher Chaloner
BACKGROUND: Specimen contamination that goes unnoticed can have many adverse consequences for patients including inappropriate investigations or treatment decisions based on erroneous results. Little is known about UK laboratory practices relating to specimen contamination therefore this national survey aimed to gather valuable baseline data. METHODS: An electronic survey consisting of 26 questions was designed to obtain key information relating to specimen contamination including its frequency, how it is indentified by laboratories, and actions taken in event of confirmed contamination...
October 29, 2018: Annals of Clinical Biochemistry
Ross M Valentine, Hayley Sharrod-Cole, Clare Ford, Rousseau Gama
No abstract text is available yet for this article.
October 29, 2018: Annals of Clinical Biochemistry
Li Ai, Jun Wang, Chang Zhao, Yonghe Tang, Weishan Wang, Yan Yu, Shengjie Zhao, Yalan Di, Xuechao Fei, Hongjun Luo, Hui Li, Wenhong Luo, Tingting Li, Rongqiao He, Weiying Lin, Zhiqian Tong
BACKGROUND: Morning urine formaldehyde (FA) levels could predict the severe degree of dementia in patients with post-stroke dementia (PSD) and Alzheimer's disease (AD). However, the routinely available technique of high-performance liquid chromatography (HPLC) for detecting urine FA requires expensive and sophisticated equipments. METHODS: We established a fluorescence spectrophotometric method by using a FA-specific fluorescent probe- NaFA (λex/em = 430/543 nm)...
October 29, 2018: Annals of Clinical Biochemistry
William A Sadler
BACKGROUND: Bland-Altman analysis is a popular and widely used method for assessing the level of agreement between two analytical methods. An important assumption is that paired method differences exhibit approximately constant (homogeneous) scatter when plotted against pair means. This allows estimation of limits of agreement which retain validity across the entire range of mean values. In practice, pair differences often increase systematically with the mean and Bland and Altman used log transformed data to achieve approximately homogeneous scatter...
October 29, 2018: Annals of Clinical Biochemistry
Asako Sato, Sayo Yada, Emiko Hosoba, Hiroko Kanno, Hitomi Miura
Background To promote glycated albumin (GA) standardization, Committee on Diabetes Mellitus Indices, Japan Society of Clinical Chemistry published the recommended reference method, and is supplying the reference standard material (JCCRM 611). In this study, we evaluated the basic performance of "standardized enzymatic method" that is traceable to JCCRM 611, and established a unit conversion formula from standardized GA value (mmol/mol) to the routinely used GA value (%) . Methods To evaluate accuracy and within-run reproducibility of the "standardized enzymatic method", JCCRM 611 was measured 20 times...
October 3, 2018: Annals of Clinical Biochemistry
Brian Shine, Julian H Barth
No abstract text is available yet for this article.
September 12, 2018: Annals of Clinical Biochemistry
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