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Journals Indian Journal of Biochemistry...

Indian Journal of Biochemistry & Biophysics

https://read.qxmd.com/read/26040112/purification-characterization-and-synthetic-application-of-a-thermally-stable-laccase-from-hexagonia-tenuis-mtcc-1119
#21
JOURNAL ARTICLE
Pankaj Kumar Chaurasia, Shashi Lata Bharati, Sudha Yadava, Rama Shanker Singh Yadav
A thermally stable laccase was purified from the culture filtrate of Hexagonia tenuis MTCC-1119. The method involved concentration of the culture filtrate by ammonium sulphate precipitation and an anion-exchange chromatography on diethylaminoethyl (DEAE) cellulose. The sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and native polyacrylamide gel electrophoresis (native-PAGE) both gave single protein bands, indicating that the enzyme preparation was pure. The molecular mass of the enzyme determined from SDS-PAGE analysis was 100 kDa...
February 2015: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/26040111/structural-analysis-and-molecular-docking-of-potential-ligands-with-chorismate-synthase-of-listeria-monocytogenes-a-novel-antibacterial-drug-target
#22
JOURNAL ARTICLE
Md Musharaf Hossain, Pradip Kumar Roy, A T M Jannatul Mosnaz, Shahriar Kabir Shakil, Md Mehedi Hasan, Shamsul H Prodhan
Listeriosis, in particular that caused by Listeria monocytogenes, is a major foodborne pathogen, and its control is becoming difficult because of widespread emergence of drug resistance strains. Chorismate synthase (CS), an essential enzyme of shikimate pathway present only in bacteria, fungi, plant and some apicomplexan parasites, is a validated potential antimicrobial drug target. Antimicrobial development through the elucidation of essential structural features of the CS of L. monocytogenes (LmCS), identification and prioritization of potential lead compounds targeted against LmCS were done...
February 2015: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/26040110/computational-prediction-of-mhc-class-i-epitopes-for-most-common-viral-diseases-in-cattle-bos-taurus
#23
JOURNAL ARTICLE
Tanmaya Kumar Sahu, A R Rao, Prabina Kumar Meher, Bishnu Charan Sahoo, Satakshi Gupta, Anil Rai
Viral diseases like foot-and-mouth disease (FMD), calf scour (CS), bovine viral diarrhea (BVD), infectious bovine rhinotracheitis (IBR) etc. affect the growth and milk production of cattle (Bos taurus) causing severe economic loss. Epitope-based vaccine designing have been evolved to provide a new strategy for therapeutic application of pathogen-specific immunity in animals. Therefore, identification of major histocompatibility complex (MHC) binding peptides as potential T-cell epitopes is widely applied in peptide vaccine designing and immunotherapy...
February 2015: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/26040109/serum-neuron-specific-enolase-and-s-100%C3%AE-levels-as-prognostic-follow-up-markers-for-oxygen-administered-carbon-monoxide-intoxication-cases
#24
JOURNAL ARTICLE
Ali Osman Yildirim, Murat Eroglu, Umit Kaldirim, Yusuf Emrah Eyi, Kemal Simsek, Murat Durusu, Levent Yamanel, Ibrahim Arziman, Salim Kemal Tuncer, Mehmet Toygar, Arzu Balkan, Tuncer Cayci, Seref Demirbas, Sukru Oter, Cumhur Bilgi
Serum neuron-specific enolase (NSE) and S-100β levels are considered novel biochemical markers of neuronal cell injury. In this study, the initial and post-treatment levels of NSE and S-100β were compared in carbon monoxide (CO) poisoning patients, who received normorbaric oxygen (NBO) or hyperbaric oxygen (HBO) therapy. Forty consecutive patients with acute CO poisoning were enrolled in this prospective, observational study. According to their clinical symptoms and observations, twenty patients were treated with NBO, and the other twenty with HBO...
February 2015: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/26040108/analyzing-resistance-pattern-of-non-small-cell-lung-cancer-to-crizotinib-using-molecular-dynamic-approaches
#25
JOURNAL ARTICLE
Anish Kumar, K Ramanathan
Crizotinib is the potential anticancer drug used for the treatment of non-small cell lung cancer (NSCLC) approved by FDA in 2011. The main target for the crizotinib is anaplastic lymphoma kinase (ALK). Evidences available indicate that double mutant ALK (L1196M and G1269A) confers resistance to crizotinib. However, how mutation confers drug resistance is not well-understood. Hence, in the present study, molecular dynamic (MD) simulation approach was employed to study the impact of crizotinib binding efficacy with ALK structures at a molecular level...
February 2015: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/26040107/effect-of-different-serine-protease-inhibitors-in-validating-the-115-kda-leishmania-donovani-secretory-serine-protease-as-chemotherapeutic-target
#26
JOURNAL ARTICLE
Tapati Chakraborti, Partha Das, Rajdeep Choudhury, Tripti De
Proteases have been considered as an important group of targets for development of antiprotozoal drugs due to their essential roles in host-parasite interactions, parasite immune evasion, life cycle transition and pathogenesis of parasitic diseases. The development of potent and selective serine protease inhibitors targeting L. donovani secretory serine protease (pSP) could pave the way to the discovery of potential antileishmanial drugs. Here, we employed different classical serine protease inhibitors (SPIs), such as aprotinin, N-tosyl-1-phenylalanine chloromethyl ketone (TPCK), N-tosyl-lysine chloromethyl ketone (TLCK), benzamidine (Bza) and pSP-antibody to determine the role of the protease in parasitic survival, growth and infectivity...
February 2015: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/26040106/beta-catenin-is-degraded-by-both-caspase-3-and-proteasomal-activity-during-resveratrol-induced-apoptosis-in-hela-cells-in-a-gsk3%C3%AE-independent-manner
#27
JOURNAL ARTICLE
Mahan Ray, Neha Rai, Kuladip Jana, Supratim Ghatak, Arnab Basu, Soumyajit Banerjee Mustafi, Sanghamitra Raha
Increased activity of β-catenin, an important transcriptional activator for survival and proliferation-associated genes has been linked with many cancers. We examined whether β-catenin is a target of resveratrol and whether its degradation contributes to the pro-apoptotic effects of resveratrol. HeLa cells were exposed to 60 μM resveratrol for 48 h. Apoptosis was confirmed by measurement of annexin V externalization, caspase-3 activation and DNA fragmentation. Induction of apoptosis was observed as early as 12 h, when both caspase-3 activation and PARP (poly ADP ribose polymerase) cleavage occurred...
February 2015: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823231/herbicide-paraquat-induces-sex-specific-variation-of-neuroinflammation-and-neurodegeneration-in-drosophila-melanogaster
#28
JOURNAL ARTICLE
Shauryabrota Dalui, Arindam Bhattacharyya
There are several reports on herbicide paraquat (PQ)-induced Parkinsonian-like pathology in different animal models, including Drosophila melanogaster. Also, the role of some inflammatory factors, such as nitric oxide is reported in PQ-induced neuroinflammation of Drosophila. Although invertebrate model is valuable to study the conserved inflammatory pathway at the time of neurodegeneration, but neuroinflammation during PQ-mediated neurodegeneration has not been studied explicitly in Drosophila. In this study, the inflammatory response was examined in Drosophila model during PQ-induced neurodegeneration...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823230/lys-413-of-s-phase-mrna-cycling-sequence-binding-protein-from-leishmania-donovani-ldcsbp-is-modified-through-monoubiquitination-that-is-responsible-for-inhibition-of-its-riboendonuclease-activity
#29
JOURNAL ARTICLE
Kasturi Guha, Sneha Das, Partha Saha
In addition to well-known process of proteasome-mediated degradation of polyubiquitinated proteins, monoubiquitination of proteins is also an important post-translational modification that regulates various non-degradative cellular processes like protein trafficking, cellular signalling, DNA replication and DNA repair. We have previously characterized a multi-domain cycling sequence binding protein LdCSBP from Leishmania donovani, which binds specifically to a conserved CAUAGAAG octamer containing RNAs via its uniquely arranged CCCH type Zn-fingers and degrades them using its Smr endonuclease domain, indicative of its potential role in the turnover of the S-phase mRNAs...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823229/influence-of-ultraviolet-c-bystander-effect-on-inflammatory-response-in-a375-cell-on-subsequent-exposure-to-ultraviolet-c-or-hydrogen-peroxide
#30
JOURNAL ARTICLE
Dipanjan Guha, Sudipta Bhowmik, Rita Ghosh
Ultraviolet C (UVC) irradiation (λ: 200-280 nm) causes release of several secretory cytokines responsible for inflammation. Our objective was to investigate whether inflammatory response was also induced in bystander cells. For this purpose, the conditioned medium containing the released factors from UVC irradiated A375 cells was used in this study to evaluate the expression of inflammatory markers, such as tumour necrosis factor alpha (TNFα), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) and p38 mitogen-activated protein kinase (p38 MAPK) in its bystander cells...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823228/leishmania-donovani-secretory-serine-protease-alters-macrophage-inflammatory-response-via-cox-2-mediated-pge-2-production
#31
JOURNAL ARTICLE
Partha Das, Tripti De, Tapati Chakraborti
Leishmania parasites determine the outcome of the infection by inducing inflammatory response that suppresses macrophage's activation. Defense against Leishmania is dependent on Th1 inflammatory response by turning off macrophages' microbicidal property by upregulation of COX-2, as well as immunosuppressive PGE-2 production. To understand the role of L. donovani secretory serine protease (pSP) in these phenomena, pSP was inhibited by its antibody and serine protease inhibitor, aprotinin. Western blot and TAME assay demonstrated that pSP antibody and aprotinin significantly inhibited protease activity in the live Leishmania cells and reduced infection index of L...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823227/tlr4-mediated-activation-of-myd88-signaling-induces-protective-immune-response-and-il-10-down-regulation-in-leishmania-donovani-infection
#32
JOURNAL ARTICLE
Joydeep Paul, Kshudiram Naskar, Sayan Chowdhury, Md Nur Alam, Tapati Chakraborti, Tripti De
In visceral leishmaniasis, a fragmentary IL-12 driven type 1 immune response along with the expansion of IL-10 producing T-cells correlates with parasite burden and pathogenesis. Successful immunotherapy involves both suppression of IL-10 production and enhancement of IL-12 and nitric oxide (NO) production. As custodians of the innate immunity, the toll-like receptors (TLRs) constitute the first line of defense against invading pathogens. The TLR-signaling cascade initiated following innate recognition of microbes shapes the adaptive immune response...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823226/challenges-of-ionizing-radiation-in-tumor-treatment-and-role-of-angiogenesis
#33
REVIEW
Subir Kumar Das
Ionizing radiation is a non-specific, but most widely used therapeutic method for cancer treatment. However, a minor fraction of tumor cell population manages to survive after radiation. Radiation efficacy depends on adequate oxygen supply. Rapid growing tumors cause hypoxia that upregulates many pro-survival pathways. At clinical doses, radiation activates inflammatory pathways and causes oxidative stress that plays a positive role during angiogenesis. Selective targeting of signaling mechanisms may radiosensitize tumors...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823225/signaling-pathways-in-insulin-and-igf-i-mediated-oocyte-maturation-in-lower-vertebrates
#34
REVIEW
Dilip Mukherjee, Suravi Majumder, Sujata Roy Moulick, Puja Pal, Buddhadeb Mallick, Arindam Chakraborty, Shreyasi Gupta
The endocrine control of oocyte maturation in fish and amphibians has proved to be a valuable model for investigating the rapid and non-genomic steroid actions at the cell surface. Considerable progress has made over the last decade in elucidating signaling pathways in steroid-induced oocyte maturation. In addition to steroids, various growth factors have also been reported to be involved in this process and progress being made to elucidate their mechanism of actions. Exposure of fully-grown oocytes to steroids or growth factors (insulin/IGFs) initiates various signaling cascade, leading to formation and activation of maturation-promoting factor (MPF), a key enzyme that catalyzes entry into M-phase of meiosis I and II...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823224/phospholipid-remodeling-and-eicosanoid-signaling-in-colon-cancer-cells
#35
REVIEW
Siddhartha Das, Leobarda Robles Martinez, Suparna Ray
Phospholipid remodeling and eicosanoid synthesis are central to lipid-based inflammatory reactions. Studies have revealed that membrane phospholipid remodeling by fatty acids through deacylation/reacylation reactions increases the risk of colorectal cancers (CRC) by allowing the cells to produce excess inflammatory eicosanoids, such as prostaglandins, thromboxanes and leukotrienes. Over the years, efforts have been made to understand the lipid remodeling pathways and to design anti-cancer drugs targeting the enzymes of eicosanoid biosynthesis...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823223/regulation-of-n-myristoyltransferase-by-the-calpain-and-caspase-systems
#36
REVIEW
Rajendra K Sharma, Sujeet Kumar, Sreejit Parameswaran, Jonathan R Dimmock
N-myristoyltransferase (NMT) is an essential eukaryotic enzyme which catalyzes the transfer of the myristoyl group to the terminal glycine residue of a number of proteins including those involved in signal transduction and apoptotic pathways. In higher eukaryotes, two isoforms of NMT have been identified (NMT1 and NMT2) which share about 76% amino acid sequence identity in humans. Protein-protein interactions of NMTs reveal that m-calpain interacts with NMT1 whereas caspase-3 interacts with NMT2. These findings reveal differential interactions of both isoforms of NMT with various signaling molecules...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823222/lung-retinoid-metabolism-and-signaling-in-chronic-obstructive-pulmonary-disease
#37
REVIEW
Salil K Das, Somdutta Sinha Roy, Shyamali Mukherjee, David E Ong
There are a number of sites that are required for the production and/or action of all-trans retinoic acid (ATRA). In particular, interruption of different components of the chain of trafficking and metabolism has been associated with cancers arising in numerous organs of the body. Preliminary work suggests that such interruptions may be a factor in lung disorders induced by the smoke exposure. The active metabolite of retinoid, ATRA offers a therapeutic strategy to protect against functional abnormality in the lung, including chronic obstructive pulmonary disease (COPD)...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823221/insulin-signaling-network-in-cancer
#38
REVIEW
Alpana Ray, Mohamed Alalem, Bimal K Ray
The primary function of insulin is viewed as a hormone that controls blood glucose level. However, there is growing evidence that aberrant insulin level and insulin-mediated signaling can lead to cancer development and progression. The insulin-cancer relationship has stemmed from various observational and epidemiological studies, which linked higher incidence of cancer with central obesity, type II diabetes and other conditions associated with increased levels of circulating insulin, insulin resistance and hyperinsulinemic states...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823220/regulation-of-cardiac-%C3%AE-3-adrenergic-receptors-in-hyperglycemia
#39
REVIEW
Belma Turan, Erkan Tuncay
Beta-adrenoceptors (β-AR), members of the G protein-coupled receptors play important roles in the regulation of heart function. A positive inotropic action of catecholamines is mediated through their interaction with β-AR, located on the sarcolemma, while they can also mediate some deleterious effects, such as cardiac arrhythmias or myocardial apoptosis. The well-known β-AR-associated signaling in heart is composed of a coupled mechanism among both β1- and β2-AR and stimulatory G protein (G(s)). This coupled mechanism further leads to the activation of adenylyl cyclase and thereby increases in intracellular cAMP level...
December 2014: Indian Journal of Biochemistry & Biophysics
https://read.qxmd.com/read/25823219/signaling-mechanisms-regulating-fibroblast-activation-phenoconversion-and-fibrosis-in-the-heart
#40
REVIEW
Jessica MacLean, Kishore B S Pasumarthi
Cardiac fibroblasts (CFs) maintain the cardiac extracellular matrix (ECM) through myocardial remodelling. The remodelling process can become dysregulated during various forms of heart disease which leads to an overall accumulation of ECM. This results in cardiac fibrosis which increases the risk of heart failure in many patients. During heart disease, quiescent CFs undergo phenoconversion to an activated cell type called cardiac myofibroblasts (CMFs). Factors influencing phenoconversion include transforming growth factor β (TGF-β) which via SMADs (small mothers against decapentaplegic) activates the myofibroblast marker gene αSMA (α smooth muscle actin)...
December 2014: Indian Journal of Biochemistry & Biophysics
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