journal
https://read.qxmd.com/read/38591530/evaluation-of-a-new-ct-ng-tv-mg-real-time-pcr-kit-vircell-versus-the-allplex-sti-essential-assay-seegene-for-the-diagnosis-of-sexually-transmitted-infections
#1
JOURNAL ARTICLE
María Aguilera-Franco, María Tarriño-León, M J Olivares-Durán, Beatriz Espadafor, Javier Rodríguez-Granger, Juan Antonio Reguera, Fernando Cobo, Antonio Sampedro, José María Navarro
Sexually transmitted infections (STI) are a public health problem. Real-time PCR assays are the most sensitive test for screening and diagnosis of these infections. The aim of this study was to evaluate a new CT/NG/TV/MG Real-Time PCR (RT-PCR) kit (Vircell) for the detection of Chamydia trachomatis , Neisseria gonorrhoeae , Mycoplasma genitalium and Trichomonas vaginalis for the diagnosis of sexual transmitted infections using the Allplex STI Essential Assay (Seegene) as the reference's method. A total of 497 samples from different anatomical sites (endocervical, urethral, rectal, pharyngeal and urine) were analysed from October 2022 to February 2023...
April 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38567642/cxcr2-perturbation-promotes-staphylococcus-aureus-implant-associated-infection
#2
JOURNAL ARTICLE
Mike Akaraphanth, Tara M Nordgren, Casey M Gries
Introduction. Staphylococcus aureus is the leading cause of acute medical implant infections, representing a significant modern medical concern. The success of S. aureus as a pathogen in these cases resides in its arsenal of virulence factors, resistance to multiple antimicrobials, mechanisms of immune modulation, and ability to rapidly form biofilms associated with implant surfaces. S. aureus device-associated, biofilm-mediated infections are often persistent and notoriously difficult to treat, skewing innate immune responses to promote chronic reoccurring infections...
April 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38567639/characterization-of-invasive-group-b-streptococcus-isolates-from-western-australian-infants-2004-2020
#3
JOURNAL ARTICLE
Ginger Hilpipre, Lucy L Furfaro, Michelle Porter, Christopher C Blyth, Daniel K Yeoh
Background. Invasive Group B Streptococcus (GBS; Streptococcus agalactiae ) remains a leading cause of infant morbidity and mortality. Intrapartum antibiotic prophylaxis (IAP) has been implemented in many countries with a reduction in early-onset disease, but an effective vaccine may further reduce the disease burden. Candidate vaccines targeting capsular polysaccharides and surface proteins are now in clinical trials. Methods. Using whole-genome sequencing and phenotypic antimicrobial susceptibility testing, we characterized sterile-site GBS isolates recovered from Western Australian infants between 2004 and 2020...
April 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38546452/exploring-the-diversity-of-vaginal-microbiota-between-healthy-women-and-cervical-cancer-patients-in-india
#4
JOURNAL ARTICLE
Ashwini Kamble, Shilpa Naik, Manju Talathi, Deepali Jadhav, Meena Sakharkar, Jian Yang, Om Prakash, Ruchika Kaul-Ghanekar
Introduction. Cervicovaginal diversity has been reported as a predictive biomarker for cervical cancer risk. We recently reported the bio-therapeutic potential of vaginal probiotics from healthy Indian women against vaginal pathogens, isolated from the invasive cervical cancer (ICC) patients. Gap Statement. The cervicovaginal microflora from cervical cancer patients has not yet been reported from Indian population. Aim. The present study aimed at comparing the cervicovaginal microbiome between healthy controls (HC) and ICC patients from the Indian population...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38535967/cancer-invasion-and-anaerobic-bacteria-new-insights-into-mechanisms
#5
JOURNAL ARTICLE
Rachel Hurst, Daniel S Brewer, Abraham Gihawi, John Wain, Colin S Cooper
There is growing evidence that altered microbiota abundance of a range of specific anaerobic bacteria are associated with cancer, including Peptoniphilus spp., Porphyromonas spp., Fusobacterium spp., Fenollaria spp., Prevotella spp., Sneathia spp ., Veillonella spp . and Anaerococcus spp. linked to multiple cancer types. In this review we explore these pathogenic associations. The mechanisms by which bacteria are known or predicted to interact with human cells are reviewed and we present an overview of the interlinked mechanisms and hypotheses of how multiple intracellular anaerobic bacterial pathogens may act together to cause host cell and tissue microenvironment changes associated with carcinogenesis and cancer cell invasion...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38530134/anthraquinones-against-cryptococcus-neoformans-sensu-stricto-antifungal-interaction-biofilm-inhibition-and-pathogenicity-in-the-caenorhabditis-elegans-model
#6
JOURNAL ARTICLE
Géssica Dos Santos Araújo, Raimunda Sâmia Nogueira Brilhante, Maria Gleiciane da Rocha, Lara de Aguiar, Débora de Souza Collares Maia Castelo-Branco, Glaucia Morgana de Melo Guedes, José Júlio Costa Sidrim, Waldemiro Aquino Pereira Neto, Marcos Fábio Gadelha Rocha
Introduction. Cryptococcal biofilms have been associated with persistent infections and antifungal resistance. Therefore, strategies, such as the association of natural compounds and antifungal drugs, have been applied for the prevention of biofilm growth. Moreover, the Caenorhabditis elegans pathogenicity model has been used to investigate the capacity to inhibit the pathogenicity of Cryptococcus neoformans sensu stricto . Hypothesis. Anthraquinones and antifungals are associated with preventing C. neoformans sensu stricto biofilm formation and disrupting these communities...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38526913/-clostridioides-difficile-detection-and-infection-in-children-are-they-just-small-adults
#7
REVIEW
Sam Watkin, Francis Yongblah, James Burton, John C Hartley, Elaine Cloutman-Green
Clostridioides difficile is a well-recognized healthcare-associated pathogen, with its significance widely recognized in adult populations. Despite this, there is limited data on the significance of detection within paediatric populations, both for individual patient management and wider transmission risk-based considerations. High rates of colonization are understood to occur in infants, with increasing levels up to 11 months, and colonization rates similar to adults by 8 years old. Sources of C...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38506718/-in-vitro-and-in-silico-assessment-of-anti-biofilm-and-anti-quorum-sensing-properties-of-2-4-di-tert-butylphenol-against-acinetobacter-baumannii
#8
JOURNAL ARTICLE
Nisha Kumari Jha, Venkadesaperumal Gopu, Chandran Sivasankar, Satya Ranjan Singh, Palanisamy Bruntha Devi, Ayaluru Murali, Prathapkumar Halady Shetty
Introduction. Acinetobacter baumannii is a nosocomial pathogen with a high potential to cause food-borne infections. It is designated as a critical pathogen by the World Health Organization due to its multi-drug resistance and mortalities reported. Biofilm governs major virulence factors, which promotes drug resistance in A. baumannii . Thus, a compound with minimum selection pressure on the pathogen can be helpful to breach biofilm-related virulence. Hypothesis/Gap Statement. To identify anti-biofilm and anti-virulent metabolites from extracts of wild Mangifera indica (mango) brine pickle bacteria that diminishes pathogenesis and resistance of A...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38506717/performance-of-metagenomic-next-generation-sequencing-in-cerebrospinal-fluid-for-diagnosis-of-tuberculous-meningitis
#9
JOURNAL ARTICLE
Bi-Wei Lin, Jian-Chen Hong, Zai-Jie Jiang, Wei-Qing Zhang, Qi-Chao Fan, Xiang-Ping Yao
Purpose. Metagenomic next-generation sequencing (mNGS) has been widely used in the diagnosis of infectious diseases, while its performance in diagnosis of tuberculous meningitis (TBM) is incompletely characterized. The aim of this study was to assess the performance of mNGS in the diagnosis of TBM, and illustrate the sensitivity and specificity of different methods. Methods. We retrospectively recruited TBM patients between January 2021 and March 2023 to evaluate the performance of mNGS on cerebrospinal fluid (CSF) samples, in comparison with conventional microbiological testing, including culturing of Mycobacterium tuberculosis (MTB), acid-fast bacillus (AFB) stain, reverse transcription PCR and Xpert MTB/RIF...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38506623/delays-to-treatment-initiation-and-emergence-of-drug-resistance-among-new-adult-tuberculosis-patients-in-tigray-northern-ethiopia
#10
JOURNAL ARTICLE
Kiros Tedla, Nega Berhe, Afework Mulugeta, Girmay Medhin, Gebretsadik Berhe, Guesh Abrha, Tilahun Teklehaymanot
Introduction. Studies in Ethiopia have indicated that tuberculosis (TB) patient's elapsed a long time before initiating treatment. Gap Statement. However, there is very limited evidence on the association of treatment initiation delay with drug resistance. Research Aim. To investigate the association of delayed treatment initiation with drug resistance among newly diagnosed TB patients in Tigray, Ethiopia. Methods. We conducted a follow-up study from October 2018 to June 2020 by recruiting 875 pulmonary tuberculosis (PTB) patients from 21 randomly selected health facilities...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38506266/-listeria-monocytogenes-the-silent-assassin
#11
JOURNAL ARTICLE
Emily T Fotopoulou, Claire Jenkins, Anaïs Painset, Corinne Amar
Listeriosis is a foodborne infection in humans caused by Listeria monocytogenes. Consumption of contaminated food can lead to severe infection in vulnerable patients, that can be fatal. Clinical manifestations include sepsis and meningitis, and in pregnancy-associated infection, miscarriage and stillbirth. Diagnosis is confirmed by culture and identification of the pathogen from blood, cerebrospinal fluid, vaginal swab, placenta or amniotic fluid. Treatment regimens recommend amoxicillin, ampicillin or an aminoglycoside...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38440953/novel-automated-antifungal-susceptibility-testing-system-for-yeasts-based-on-dual-detection-algorithm-of-turbidimetry-and-colorimetry
#12
JOURNAL ARTICLE
Tingting Shen, Piao Liu, Dongmiao Chen, Duoduo Liu, Juan Fang, Qian Wang, Yinjuan Xu, Chunjuan Tan, Congrong Wang
Introduction. The increasing prevalence and growing resistance of fungi present a significant peril to public health. There are only four classes of antifungal medicines available today, and few candidates are in clinical trials. Hypothesis/Gap Statement. Rapid and sensitive diagnostic techniques are lacking for most fungal pathogens, and those that do exist are expensive or hard to obtain. Aim. This study aimed to evaluate the feasibility of a novel automated antifungal susceptibility testing system, Fungus AST, in comparison to the broth microdilution method (BMD) recommended by the Clinical and Laboratory Standards Institute (CLSI)...
March 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38420890/improving-pulmonary-infection-diagnosis-with-metagenomic-next-generation-sequencing-of-bronchoalveolar-lavage-fluid
#13
JOURNAL ARTICLE
Ying Long, Xianzhu Xia, Hao Feng, Pingsen Zhao
Introduction. Inappropriate use of antibiotics and inadequate therapeutic regimens for early-stage pulmonary infections are major contributors to increased prevalence of complications and mortality. Moreover, due to the limitations in sensitivity of conventional testing, there is an urgent need for more diagnostically efficient methods for the detection and characterization of pathogens in pulmonary infections. Hypothesis/Gap Statement. Metagenomic next-generation sequencing (mNGS) can contribute to the diagnosis and management of pulmonary infections...
February 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38415707/rapid-determination-of-antimicrobial-susceptibility-of-gram-negative-bacteria-from-clinical-blood-cultures-using-a-scattered-light-integrated-collection-device
#14
JOURNAL ARTICLE
Kerry Falconer, Robert Hammond, Benjamin J Parcell, Stephen H Gillespie
Background. A bloodstream infection (BSI) presents a complex and serious health problem, a problem that is being exacerbated by increasing antimicrobial resistance (AMR). Gap Statement. The current turnaround times (TATs) for most antimicrobial susceptibility testing (AST) methods offer results retrospective of treatment decisions, and this limits the impact AST can have on antibiotic prescribing and patient care. Progress must be made towards rapid BSI diagnosis and AST to improve antimicrobial stewardship and reduce preventable deaths from BSIs...
February 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38385528/promising-activity-of-etomidate-against-mixed-biofilms-of-fluconazole-resistant-candida-albicans-and-methicillin-resistant-staphylococcus-aureus
#15
JOURNAL ARTICLE
Cecília Rocha da Silva, Júlia Délis de Oliveira Rebouças, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Amanda Dias Barbosa, Lara Elloyse Almeida Moreira, Fátima Daiana Dias Barroso, Tatiana do Nascimento Paiva Coutinho, Elaine Aires de Lima, Claudia Roberta de Andrade, João Batista de Andrade Neto, Iri Sandro Pampolha Lima, Hélio Vitoriano Nobre Júnior, Lívia Gurgel do Amaral Valente Sá
Introduction. Candida albicans and Staphylococcus aureus are recognized for their development of resistance and biofilm formation. New therapeutic alternatives are necessary in this context. Hypothesis. Etomidate shows potential application in catheters against mixed biofilms of fluconazole-resistant C. albicans and methicillin-resistant S. aureus (MRSA). Aim. The present study aimed to evaluate the activity of etomidate against mixed biofilms of fluconazole-resistant C. albicans and MRSA. Methodology. The action of etomidate against mature biofilms was verified through the evaluation of biomass and cell viability, and its ability to prevent biofilm formation in peripheral venous catheters was determined based on counts of colony forming units (c...
February 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38380521/five-year-species-distribution-and-antimicrobial-susceptibility-of-non-tuberculous-mycobacteria-in-malaysia-2018-2022
#16
JOURNAL ARTICLE
Mahfuzah Mohamad Azranyi, Zirwatul Adilah Aziz, Dayana Ishak, Nur Farahanis Mohd Nais, Zaidatul Akmar Elias, Nurrul Atiqiah Fitri Sulaiman, Khairul Azan Hashim, Rohani Ismail, Nik Jasmin Nik Mahir, Yun Fong Ngeow, Thaw Zin
Introduction. Non-tuberculous Mycobacteria (NTM) is a group of mycobacteria distinct from the Mycobacterium tuberculosis complex. They can cause opportunistic infections, especially in immunocompromised individuals. Gap Statement. Over the last few years, there has been a growing concern regarding the distribution and antimicrobial resistance of NTM in Malaysia. however, a comprehensive study to fully grasp the NTM situation has yet to be conducted. Aim. This study aimed to investigate the species distribution and antimicrobial susceptibility patterns of NTM isolated from clinical samples in Malaysia from 2018 to 2022...
February 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38375878/high-levels-of-multidrug-resistant-isolates-of-genetically-similar-salmonella-1-4-5-12-i-from-brazil-between-1983-and-2020
#17
JOURNAL ARTICLE
Giovana do Nascimento Pereira, Amanda Aparecida Seribelli, Fábio Campioni, Carolina Nogueira Gomes, Monique Ribeiro Tiba-Casas, Marta Inês Cazentini Medeiros, Dália Dos Prazeres Rodrigues, Juliana Pfrimer Falcão
Introduction. Salmonella 1,4, [5],12:i:- strains with different antimicrobial resistance profiles have been associated with foodborne disease outbreaks in several countries. In Brazil, S . 1,4, [5],12:i:- was identified as one of the most prevalent serovars in São Paulo State during 2004-2020. Gap Statement. However, few studies have characterized this serovar in Brazil. Aim. This study aimed to determine the antimicrobial resistance profiles of S . 1,4, [5],12:i:- strains isolated from different sources in Southeast Brazil and compare their genetic diversity...
February 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38362924/bioarchaeological-investigation-of-individuals-with-suspected-multibacillary-leprosy-from-the-mediaeval-leprosarium-of-st-mary-magdalen-winchester-hampshire-uk
#18
JOURNAL ARTICLE
G Michael Taylor, Katie White-Iribhogbe, Garrard Cole, David Ashby, Graham R Stewart, Heidi Dawson-Hobbis
Introduction. We have examined four burials from the St Mary Magdalen mediaeval leprosarium cemetery in Winchester, Hampshire, UK. One (Sk.8) was a male child, two (Sk.45 and Sk.52) were adolescent females and the fourth (Sk.512) was an adult male. The cemetery was in use between the 10th and 12th centuries. All showed skeletal lesions of leprosy. Additionally, one of the two females (Sk.45) had lesions suggestive of multi-cystic tuberculosis and the second (Sk.52) of leprogenic odontodysplasia (LO), a rare malformation of the roots of the permanent maxillary incisors...
February 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38362908/comparative-evaluation-of-the-detection-rate-workflow-and-associated-costs-of-a-multiplex-pcr-panel-versus-conventional-methods-in-diagnosis-of-infectious-gastroenteritis
#19
JOURNAL ARTICLE
J Ambrosius-Eichner, M Hogardt, A Berger, G Dultz, R Idris, V A J Kempf, T A Wichelhaus
Introduction. Infectious gastroenteritis is a common reason for consulting a physician. Although most cases of gastrointestinal illness are self-limiting, the identification of the etiologic pathogen by stool specimen analysis is important in cases of more severe illness and for epidemiological reasons.Due to the broad range of causative pathogens, the conventional examination of a stool specimen is labour-intensive and usually requires different diagnostic methods. Multiplex PCR tests [e.g. BioFire Gastrointestinal (GI) Panel] allow the rapid detecting of up to 22 pathogens in one test...
February 2024: Journal of Medical Microbiology
https://read.qxmd.com/read/38362900/genomic-and-metabolic-versatility-of-pseudomonas-aeruginosa-contributes-to-its-inter-kingdom-transmission-and-survival
#20
JOURNAL ARTICLE
Sakthivel Ambreetha, Diansy Zincke, Dananjeyan Balachandar, Kalai Mathee
Pseudomonas aeruginosa is one of the most versatile bacteria with renowned pathogenicity and extensive drug resistance. The diverse habitats of this bacterium include fresh, saline and drainage waters, soil, moist surfaces, taps, showerheads, pipelines, medical implants, nematodes, insects, plants, animals, birds and humans. The arsenal of virulence factors produced by P. aeruginosa includes pyocyanin, rhamnolipids, siderophores, lytic enzymes, toxins and polysaccharides. All these virulent elements coupled with intrinsic, adaptive and acquired antibiotic resistance facilitate persistent colonization and lethal infections in different hosts...
February 2024: Journal of Medical Microbiology
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