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Tissue & Cell

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https://read.qxmd.com/read/30736911/apelin-receptor-aplnr-signaling-promotes-fibroblast-migration
#1
Ayşegül Doğan
Cell migration is one of the major cellular processes in development, tissue regeneration, wound healing, cancer and immune function. Underlying molecular mechanism behind the fibroblast cell migration and matrix interactions generates the basis of tissue homeostasis. The role of Apelin receptor (Aplnr) signaling in gastrulation movements has emerged in recent years but how Aplnr regulates cell movement remains unclear. In the current study, the migratory activity of Aplnr signaling has been shown in cultured fibroblast cells in vitro by scratch assay, gene and protein expression analyses...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736910/modeling-traumatic-injury-in-organotypic-spinal-cord-slice-culture-obtained-from-adult-rat
#2
Sareh Pandamooz, Mohammad Saied Salehi, Mohammad Ismail Zibaii, Anahid Safari, Mohammad Nabiuni, Abolhassan Ahmadiani, Leila Dargahi
Nowadays there are various models of spinal cord injury (SCI) that recreate mechanisms of human SCI. The ex vivo modeling of injury is a robust approach, confronts with less experimental and ethical challenges. Currently almost all ex vivo models are obtained either from embryonic or postnatal animals, which can hardly mimic features of human SCI. This study was designed to develop SCI in slice culture of adult rats. Here, the lumbar enlargement of an adult rat was sliced and cultured. After seven days in vitro, a weight was dropped to simulate the injury...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736909/an-improved-and-easy-protocol-for-primary-epithelial-cell-culture-from-atretic-tissue-in-biliary-atresia
#3
Sanghamitra Machhua, Yashwant Kumar, Ravi Prakash Kanojia, Alka Bhatia, Babu Ram Thapa, Ranjana Walker Minz, Anil Kumar Ram, Veena Dhawan
Biliary atresia (BA) is a lethal disease of infancy with obscure etiology. Insight into the pathogenesis of this disorder is limited by lack of availability of adequate epithelial tissue. Primary culture of human biliary epithelium may help to provide material for diagnostic and research purposes. However, culture of these cells from atretic tissue is a challenging task. We aimed to develop a reliable and easier protocol for culture of human biliary epithelial cells from excised atretic extrahepatic bile duct...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736908/macroautophagy-is-involved-in-residual-bodies-formation-during-spermatogenesis-in-sea-urchins-strongylocentrotus-intermedius
#4
Alexander V Kalachev, Olga V Yurchenko, Konstantin V Kiselev
An ultrastructural study of developing spermatids in sea urchins, Strongylocentrotus intermedius, showed that macroautophagy is involved in formation of residual bodies and removal of excessive cytoplasm by spermatids during spermatogenesis in this species. During late stages of spermatogenesis spermatids sequester excessive cytoplasm into vesicles, surrounded by a double membrane. Subsequently, these vesicles fused to one another into larger vacuoles, up to 1.5 μm in diameter. Finally, the vacuoles transformed into residual bodies by condensing their content into finely granular material of varying electron density, separated from cytoplasm by a single membrane...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736907/non-integrin-laminin-receptors-in-epithelia
#5
REVIEW
Gabriel Cloutier, Amélie Sallenbach-Morrissette, Jean-François Beaulieu
The interactions between cells and the extracellular matrix (ECM) play a major role in normal and pathological conditions. The ECM can modulate several biological functions including cell proliferation, adhesion, differentiation and survival through its interactions with cell receptors. Laminins are one of the most important glycoproteins present in basement membranes, a type of ECM. The pattern of expression of its different isoforms depends on the spatiotemporal organization of each tissue. While integrins are the most studied laminin receptors, other non-integrin laminin receptors are also involved...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736906/morphology-of-nutrient-storage-cells-in-the-gonadal-area-of-the-pacific-oyster-crassostrea-gigas-thunberg-1793
#6
Olga V Yurchenko, Alexander V Kalachev
Successful gametogenesis in invertebrates is tightly associated with functioning of specific nutrient-storing cells. In oysters, cells of vesicular connective tissue (VCT-cells), also referred to as storage cells, which form a meshwork around gonadal acini, are the major population of cells that accumulate and provide nutrients for developing gametes. During the annual reproductive cycle, populations of developing germ cells and VCT-cells demonstrate the inversely proportional size dynamics: the larger the acini, the smaller the VCT-cells...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736905/multilineage-potential-research-on-pancreatic-mesenchymal-stem-cells-of-bovine
#7
Fan Gao, Yangnan Wu, Hebao Wen, Wanwan Zhu, Han Ren, Weijun Guan, Xiuzhi Tian
Stem cells are most likely to solve all three of diabetes's problems at once, but the previous studies have mostly focused on bone marrow mesenchymal stem cells (MSCs) and adipose tissue-derived MSCs, and few studies have been done on pancreatic MSCs. In this study, pancreatic was collected to isolate MSCs from bovine, and then their biological characteristics such as growth kinetics, surface antigen, and multilineage potential were examined. Pancreatic MSCs of bovine (B-PMSCs) could be cultured for 65 passages in vitro...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736904/high-efficient-differentiation-of-human-adipose-derived-stem-cells-into-retinal-pigment-epithelium-like-cells-in-medium-containing-small-molecules-inducers-with-a-simple-method
#8
Hamid Aboutaleb Kadkhodaeian, Amir Salati, Alireza Lashay
BACKGROUND: The induction of retinal pigmented epithelium cells (RPE) is one of the most important objectives in research focused on treating retinal degenerative diseases. The present study aims to differentiate human adipose stem cells (hADSCs) into RPE cells for replacement therapies in cases of retinal degenerative diseases. METHODS: Lipoaspirate-derived human adipose stem cells (LA-hADSCs) were obtained from abdominal samples and examined by immunocytochemistry for the expression of mesenchymal adipose stem cell markers...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736903/the-effect-of-docosahexaenoic-acid-on-apelin-distribution-of-nervous-system-in-the-experimental-mouse-model-of-parkinson-s-disease
#9
Nuray Acar, Hande Parlak, Ayse Ozkan, Hakan Soylu, Sema Avcı, Ismail Ustunel, V Nimet Izgut-Uysal, Aysel Agar
Parkinson's disease (PD) is a degenerative disorder of the human central and peripheral nervous systems. n-3 fatty acids docosahexaenoic acid (DHA, C22: 6n-3) and eicosapentaenoic acid (EPA) as well as apelin have anti-inflammatory effects in various cells. At the same time, apelin has anti-oxidative and anti-apoptotic effects. The study was conducted to determine the effect of DHA on the distribution of apelin and apelin receptor (APJ) in the central nervous system in 1-methyl-4-phenyl-1, 2, 3, 6 tetrahydropyridine (MPTP)-induced PD model...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736902/reactivity-of-microglia-and-astrocytes-after-an-excitotoxic-injury-induced-by-kainic-acid-in-the-rat-spinal-cord
#10
Carolina Natalia Zanuzzi, Fabián Nishida, María Susana Sisti, Claudio Gustavo Barbeito, Enrique Leo Portiansky
After injury of the nervous system glial cells react according to the stimuli by modifying their morphology and function. Glia activation was reported in different kainic acid (KA)-induced neurodegeneration models. Here, we describe glial morphometric changes occurring in an excitotoxic KA-induced cervical spinal cord injury model. Concomitant degenerative and apoptotic processes are also reported. Male rats injected at the spinal cord C5 segment either with KA or saline were euthanized at post-injection (PI) days 1, 2, 3 or 7...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736901/adipose-derived-mesenchymal-stem-cells-improve-the-structure-and-function-of-autografted-mice-ovaries-through-reducing-oxidative-stress-and-inflammation-a-stereological-and-biochemical-analysis
#11
E Shojafar, M Soleimani Mehranjani, S M A Shariatzadeh
The aim was to investigate the effect of injecting adipose derived mesenchymal stem cells (ADSCs) on reducing oxidative stress, inflammation and improving the structure and function of the autografted ovaries through stereological and biochemical evaluations. Mice (4-5 weeks old) were divided into three groups: control, autograft, and autograft + ADSCs (six mice per group). 7 days after ovary autografting and ADSCs injection, serum concentrations of IL-6, TNF-α and IL-10, malondialdehyde and superoxide dismutase activity were measured...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736900/anti-fibrotic-effect-of-black-tea-camellia-sinensis-extract-in-experimental-pulmonary-fibrosis
#12
Kaustav Chakraborty, Avijit Dey, Arindam Bhattacharyya, Subir Chandra Dasgupta
There is no effective therapy exists for Idiopathic pulmonary fibrosis (IPF) till now. Few studies have been done on protective effects of green tea in pulmonary fibrosis but there is no single report on black tea extract (BTE) in pulmonary fibrosis so far. This study aims to investigate the anti-fibrotic effect of BTE against experimental pulmonary fibrosis. Four groups of animals were selected for this study. Group 1: control group mice. Group 2: mice exposed to bleomycin for 21 days, Group 3 and Group 4: bleomycin exposed mice treated with 25 mg BTE/kg b...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736899/dimethyl-fumarate-up-regulates-expression-of-major-neurotrophic-factors-in-the-epidermal-neural-crest-stem-cells
#13
Mohammad Saied Salehi, Afshin Borhani-Haghighi, Sareh Pandamooz, Anahid Safari, Leila Dargahi, Mehdi Dianatpour, Nader Tanideh
There is an agreement that combining treatments can lead to substantial improvement, therefore the present study assessed the effects of different concentrations of dimethyl fumarate (DMF) on viability of epidermal neural crest stem cells (EPI-NCSCs). In addition, this investigation was designed to evaluate the effects of DMF on relative expression of major trophic factors mainly the ones with neurotrophic effects, expressed in EPI-NCSCs in order to enhance their therapeutic potential. To determine the appropriate concentration of DMF for EPI-NCSCs treatment, the MTT assay was employed and based on the obtained data, EPI-NCSCs treated with 10μM DMF for 6, 24, 72 or 168 h...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736898/allogeneic-platelet-rich-plasma-serves-as-a-scaffold-for-articular-cartilage-derived-chondroprogenitors
#14
Elizabeth Vinod, Deepak Vinod Francis, Soosai Manickam Amirtham, Solomon Sathishkumar, P R J V C Boopalan
Limited self-restorative ability of the cartilage has necessitated the use of cell and tissue engineering based therapies. Recent advances in the isolation, expansion and characterization of articular cartilage derived chondroprogenitors(CPs) has gained popularity in its role for cartilage repair. Platelet rich plasma (PRP) is a reliable biological scaffold for in-vitro and in-vivo studies with reported therapeutic applications in cartilage and bone pathologies. The aim of this study was to evaluate whether human allogeneic PRP could serve as a biological scaffold for chondroprogenitors (CPs) in cartilage repair...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30736897/merkel-cells-immunohistochemical-study-in-striped-dolphin-stenella-coeruleoalba-skin
#15
E R Lauriano, S Pergolizzi, M Aragona, N Spanò, M C Guerrera, G Capillo, C Faggio
Cetacean mechanical senses, such as hearing, echolocation, active touch and the perception of water movements, are essential for their survival. Dolphins skin possesses dense packing of dermal papillae associated with the cutaneous ridges that suggests a sensory function, furthermore they are well innervated and very sensitive to touch. This is mediated by mechanoreceptors, abundant in the region of the head and in the dorsal part of the body. Most odontocetes possess vibrissae (i.e., sensory hair) that have been well described in literature and present a microanatomy similar to that of terrestrial mammals...
February 2019: Tissue & Cell
https://read.qxmd.com/read/30503065/structural-and-molecular-analysis-of-the-cancer-prostate-cell-line-pc3-oocyte-zona-pellucida-glycoproteins
#16
Jéssica Costa, Rute Pereira, Jorge Oliveira, Ângela Alves, Ângela Marques-Magalhães, Amaro Frutuoso, Carla Leal, Nuno Barros, Rui Fernandes, Diogo Queiroz Almeida, Márcia Barreiro, Alberto Barros, Mário Sousa, Rosália Sá
The human oocyte zona pellucida (ZP) is made of four glycoproteins, ZP1-ZP4. Recently, the prostate adenocarcinoma and prostate cancer PC3 cell-line were shown to express the human oocyte ZP3 glycoprotein, which was evaluated in a single report subject to patent. To further clarify whether oocyte zona pellucida glycoproteins are expressed in prostate cancer tissue and PC3-cells, in this report we evaluated protein expression of the four ZP glycoproteins in normal prostate tissue, prostate adenocarcinoma tissue and PC3-cells, and performed quantitative mRNA expression of the four ZP glycoproteins in the PC3 cell-line...
December 2018: Tissue & Cell
https://read.qxmd.com/read/30503064/fabrication-and-evaluation-of-non-mulberry-silk-fibroin-fiber-reinforced-chitosan-based-porous-composite-scaffold-for-cartilage-tissue-engineering
#17
B N Singh, K Pramanik
Lack of potential regenerative medicine to reconstruct damaged cartilage tissue has accelerated investigation and development of potential biomaterial for cartilage tissue engineering. In this study, we fabricated micron-sized non-mulberry silk fibroin fiber (SFF) using N,N-Dimethylacetamide (DMAC )/10% LiBr solution and further used to develop SFF reinforced chitosan(CH) based porous scaffold with desired pore size, porosity, swelling and structural stability. The developed scaffold was characterized for its various physico-chemical, mechanical and biological properties...
December 2018: Tissue & Cell
https://read.qxmd.com/read/30503063/chronic-ethanol-forced-administration-from-adolescence-to-adulthood-reduces-cell-density-in-the-rat-spinal-cord
#18
Rafael Monteiro Fernandes, Priscila Cunha Nascimento, Leonardo Oliveira Bittencourt, Ana Carolina Alves de Oliveira, Bruna Puty, Luana Ketlen Reis Leão, Luanna Melo Pereira Fernandes, Sabrina Cartágenes, Cristiane Socorro Ferraz Maia, Rafael Rodrigues Lima
Ethanol (EtOH) consumption is a risk factor for central nervous system damage, especially during adolescence. This study aimed to investigate the possible effects of chronic EtOH forced administration on gray and white matter of the spinal cord, from adolescence to adulthood. For this, male Wistar rats were administered EtOH by gavage (6.5 g/kg/day; 22.5% w/v) from the 35th to the 90th day of life, while control animals received only distilled water. After exposure, animals were euthanized and their spinal cords processed to obtain cervical and thoracic segments for histological analyses...
December 2018: Tissue & Cell
https://read.qxmd.com/read/30503062/cytochemical-patterns-of-the-peripheral-blood-cells-in-chinese-alligator-alligator-sinensis
#19
Xianxian Chen, Qinqin Wei, Jing Wang, Fei Peng, En Li, YongKang Zhou, Shengzhou Zhang
The Chinese alligator (Alligator sinensis) is an endemic rare crocodilian species in China. In this study, we investigated the cytochemical patterns of peripheral blood cells in Chinese alligators for the first time by a range of cytochemical staining techniques including periodic acid-Schiff(PAS), sudan black B(SBB), peroxidase(POX), alkaline phosphatase(AKP), acid phosphatase(ACP), chloroacetic acid AS-D naphthol esterase(AS-D) and α-naphthol acetate esterase(α-NAE) staining. Erythrocytes were positive for PAS, and negative for all the other staining; heterophils were strongly positive for SBB, POX, ACP, AKP, AS-D and α-NAE, while weakly positive for PAS staining; eosinophils were strongly positive for PAS, POX, AKP, ACP and AS-D, and weakly positive for SBB and α-NAE staining; basophils were strongly positive for PAS, positive for POX, ACP, AKP and α-NAE, and weakly positive for AS-D, while negative for SBB staining; monocytes were weakly positive for PAS, ACP, AKP and α-NAE, while negative for SBB, POX and AS-D staining; lymphocytes were weakly positive for PAS and α-NAE, negative for all the other staining; thrombocytes were weakly positive for PAS, and negative for all the other staining...
December 2018: Tissue & Cell
https://read.qxmd.com/read/30503061/dyrk1a-promotes-the-proliferation-migration-and-invasion-of-fibroblast-like-synoviocytes-in-rheumatoid-arthritis-via-down-regulating-spry2-and-activating-the-erk-mapk-pathway
#20
Xiaofeng Guo, Dongmei Zhang, Xing Zhang, Jiawei Jiang, Pengfei Xue, Chunshuai Wu, Jinlong Zhang, Guohua Jin, Zhen Huang, Jian Yang, Xinhui Zhu, Wei Liu, Guanhua Xu, Zhiming Cui, Guofeng Bao
Fibroblast-like synoviocytes (FLSs) play an essential role in rheumatoid arthritis (RA) by promoting synovitis, pannus growth and cartilage/bone destruction. Increased proliferation, migration and invasion of FLSs greatly contribute to RA initiation and progression. Dual-specificity tyrosine-regulated kinase 1A (Dyrk1A), a serine/threonine kinase, regulates MAPK pathway activation, and governs the proliferation and differentiation of neuronal progenitor cells and cancer cells. Till now, the expression and possible function of Dyrk1A in RA FLSs have not been explored...
December 2018: Tissue & Cell
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