Asrafun Nahar, John Becker, Rolando Pasquariello, Jason Herrick, Heather Rogers, Mingxiang Zhang, William Schoolcraft, Rebecca L Krisher, Ye Yuan
Chemically defined oocyte maturation media supplemented with FGF2, LIF and IGF-1 (FLI medium) enabled significantly improved oocyte quality in multiple farm animals, yet the molecular mechanisms behind such benefits were poorly defined. Here, we first demonstrated that FLI medium enhanced mouse oocyte quality assessed by blastocyst formation after in vitro fertilization, and implantation and fetal development after embryo transfer. We then analyzed the glucose concentrations in the spent media, ROS concentrations, mitochondrial membrane potential, spindle morphology in oocytes, and the abundance of transcripts of EGF-like factors, cumulus expansion factors, and glucose metabolism related genes in cumulus cells...
January 23, 2024: Biology of Reproduction