journal
https://read.qxmd.com/read/38376238/inefficient-sox9-upregulation-and-absence-of-rspo1-repression-lead-to-sex-reversal-in-the-b6-xy-tir-mouse-gonad
#21
JOURNAL ARTICLE
Jiangqin Cao, Fatima El Mansouri, Sofia Reynoso, Zongping Liu, Jiaqiao Zhu, Teruko Taketo
Sry on the Y-chromosome upregulates Sox9, which in turn upregulates a set of genes such as Fgf9 to initiate testicular differentiation in the XY gonad. In the absence of Sry expression, genes such as Rspo1, Foxl2, and Runx1 support ovarian differentiation in the XX gonad. These two pathways antagonize each other to ensure the development of only one gonadal sex in normal development. In the B6.YTIR mouse, carrying the YTIR-chromosome on the B6 genetic background, Sry is expressed in a comparable manner with that in the B6...
February 20, 2024: Biology of Reproduction
https://read.qxmd.com/read/38374691/igfbp1a-is-a-nutrient-deficient-response-factor-that-can-inhibit-fish-reproduction-through-the-hypothalamus-pituitary-ovary-axis%C3%A2
#22
JOURNAL ARTICLE
Wei Li, Hangyu Li, Qiongyao Hu, Linlin Wang, Zhan Yin, Guangfu Hu
Reproduction is a high energy consuming process, so long-term malnutrition can significantly inhibit gonadal development. However, little is known about the molecular mechanism by which fasting inhibits reproduction. Our present study found that fasting could dramatically induce insulin-like growth factor binding protein 1 (IGFBP1) expression in the liver, hypothalamus, pituitary and ovaries of grass carp. In addition, IGFBP1a in the hypothalamus-pituitary-gonad axis could inhibit the development of gonads...
February 19, 2024: Biology of Reproduction
https://read.qxmd.com/read/38335245/membrane-inflammasome-activation-by-choriodecidual-ureaplasma-parvum-infection-without-intra-amniotic-infection-in-an-nhp-model
#23
JOURNAL ARTICLE
Sudeshna Tripathy, Irina Burd, Meredith A Kelleher
Intrauterine infection is a significant cause of preterm labor and neonatal morbidity and mortality. Ureaplasma parvum is the microorganism most commonly isolated from cases of preterm birth and preterm premature rupture of membranes (pPROM). However, the mechanisms during the early stages of ascending reproductive tract infection remain poorly understood. To examine inflammation in fetal (chorioamnionic) membranes in response to U. parvum infection, we utilized a non-human primate (NHP) model of choriodecidual infection...
February 9, 2024: Biology of Reproduction
https://read.qxmd.com/read/38330185/establishment-and-comparison-of-human-term-placenta-derived-trophoblast-cells
#24
JOURNAL ARTICLE
Manuel S Vidal, Enkhtuya Radnaa, Natasha Vora, Kamil Khanipov, Cristina Antich, Marc Ferrer, Rheanna Urrabaz-Garza, Jeena E Jacob, Ramkumar Menon
Research on the biology of fetal-maternal barriers has been limited by access to physiologically relevant cells, including trophoblast cells. In this study, we describe the development of a human-term placenta-derived cytotrophoblast immortalized cell line (hPTCCTB) derived from the basal plate. hPTCCTB cells are comparable to their primary cells of origin in terms of morphology, marker expression, and functional responses. We demonstrate that these can transform into syncytiotrophoblast and extravillous trophoblasts...
February 8, 2024: Biology of Reproduction
https://read.qxmd.com/read/38320204/cyclophosphamide-activates-ferroptosis-induced-dysfunction-of-leydig-cells-via-smad2-pathway
#25
JOURNAL ARTICLE
Senlin Liao, Cun Wei, Guanyang Wei, Haoyu Liang, Fan Peng, Lei Zhao, Ziguang Li, Cundong Liu, Qizhao Zhou
Cyclophosphamide (CP) is a widely used chemotherapeutic drug and immunosuppressant in the clinic, and the hypoandrogenism caused by CP is receiving more attention. Some studies found that ferroptosis is a new mechanism of cell death closely related to chemotherapeutic drugs and plays a key role in regulating reproductive injuries. The purpose of this study is to explore ferroptosis' role in testicular Leydig cell dysfunction and molecular mechanisms relating to it. In this study, The level of ferroptosis in the mouse model of testicular Leydig cell dysfunction induced by CP was significantly increased and further affected testosterone synthesis...
February 6, 2024: Biology of Reproduction
https://read.qxmd.com/read/38320203/bta-mir-301a-targets-acvr1-to-influence-cleavage-time-and-blastocyst-formation-rate-of-early-embryos-in-cattle
#26
JOURNAL ARTICLE
Heqiang Li, Jing Gao, Jingyi Wang, Baobao Zhao, Xiaonan Ma, Bihan Zhang, Changrong Lv, Hailian Qiao, Yongsheng Wang, Suzhu Qing
Accumulating evidence indicates that paternally-derived miRNAs play a crucial role in the development of early embryos and are regarded as the key factor in the successful development of somatic cell cloned embryos. In our previous study, bta-miR-301a was found to be highly expressed in bovine sperm, and was delivered into oocytes during fertilization. In this study, bioinformatics, dual luciferase reporter assays, rescue experiments and gain- and loss-of-function experiments indicated that ACVR1 is the target gene of bta-miR-301a in early bovine embryos...
February 6, 2024: Biology of Reproduction
https://read.qxmd.com/read/38315794/new-uses-for-an-old-technique-live-imaging-on-the-slice-organ-culture-to-study-reproductive-processes
#27
JOURNAL ARTICLE
Ciro Maurizio Amato, Humphrey Hung-Chang Yao
Reproductive processes are dynamic and involve extensive morphological remodeling and cell-cell interactions. Live imaging of organs enhances our understanding of how biological processes occur in real time. Slice culture is a type of organ culture where thick slices are collected from an organ and cultured for several days. Slice culture is a useful and easy-to-implement technique for live imaging of reproductive events at cellular resolution. Here we describe a pipeline of live imaging on slice culture to visualize the process of urethra closure in mouse embryonic penis as a proof of principle...
February 5, 2024: Biology of Reproduction
https://read.qxmd.com/read/38302090/an-interview-with-dr-daniel-johnston
#28
JOURNAL ARTICLE
T Rajendra Kumar
No abstract text is available yet for this article.
February 1, 2024: Biology of Reproduction
https://read.qxmd.com/read/38288660/foxp2-deficiency-impairs-reproduction-by-modulating-the-hypothalamic-pituitary-gonadal-axis-in-zebrafish
#29
JOURNAL ARTICLE
Maya Wang, Juanjuan Luo, Lu Dai, Meilan Feng, Xiaoqian Cao, Jiannan Zhang, Yiping Wan, Xiaojun Yang, Yajun Wang
FOXP2 was initially characterized as a transcription factor linked to speech and language disorders. Single-cell RNA sequencing (scRNA-seq) reveals that Foxp2 is enriched in the gonadotrope cluster of the pituitary gland and colocalized with the hormones LHB and FSHB in chickens and mice, implying that FOXP2 might be associated with reproduction in vertebrates. Herein, we investigated the roles of foxp2 in reproduction in a Foxp2-deficient zebrafish model. The results indicated that the loss of Foxp2 inhibits courtship behavior in adult male zebrafish...
January 30, 2024: Biology of Reproduction
https://read.qxmd.com/read/38281153/intronless-pabpc6-encodes-a-testis-specific-cytoplasmic-poly-a-binding-protein-but-is-dispensable-for-spermatogenesis-in-the-mouse
#30
JOURNAL ARTICLE
Yuko Kaku, Yuka Isono, Hideto Tanaka, Tomohiro Kobayashi, Yoshinori Kanemori, Shin-Ichi Kashiwabara
Besides ubiquitous poly(A)-binding protein, cytoplasmic 1 (PABPC1), testis-specific PABPC2/PABPt (in humans, referred to as PABPC3), and female and male germline-specific PABPC1L/ePAB, have been reported in the mouse testis. Recent in silico analysis additionally identified testis-specific Pabpc6 in the mouse. In this study, we characterized PABPC6 and its mutant mice. PABPC6 was initially detectable in the cytoplasm of pachytene spermatocytes, increased in abundance in round spermatids, and decreased in elongating spermatids...
January 28, 2024: Biology of Reproduction
https://read.qxmd.com/read/38271627/high-throughput-mrna-seq-atlas-of-human-placenta-shows-vast-transcriptome-remodeling-from-first-to-third-trimester
#31
JOURNAL ARTICLE
Tania L Gonzalez, Sahar Wertheimer, Amy E Flowers, Yizhou Wang, Chintda Santiskulvong, Ekaterina L Clark, Caroline A Jefferies, Kate Lawrenson, Jessica L Chan, Nikhil V Joshi, Yazhen Zhu, Hsian-Rong Tseng, S Ananth Karumanchi, John Williams, Margareta D Pisarska
The placenta, composed of chorionic villi, changes dramatically across gestation. Understanding differences in ongoing pregnancies are essential to identify the role of chorionic villi at specific times in gestation and develop biomarkers and prognostic indicators of maternal-fetal health. The normative mRNA profile is established using next-generation sequencing of 124 first trimester and 43 third trimester human placentas from ongoing healthy pregnancies. Stably expressed genes not different between trimesters and with low variability are identified...
January 25, 2024: Biology of Reproduction
https://read.qxmd.com/read/38271626/activation-of-interleukin33-nf%C3%AE%C2%BAb-axis-in-granulosa-cells-during-atresia-and-its-role-in-disposal-of-atretic-follicles
#32
JOURNAL ARTICLE
Jean Wu, Colin Carlock, Kiana Tatum, Junbo Shim, Cindy Zhou, Yahuan Lou
It has been previously shown that the cytokine interleukin33 (IL33) is required for two processes, i.e. autophagic digestion of granulosa cells and recruitment of macrophages into atretic follicles, for full disposal of atretic follicles. Now, this study shows that activation of IL33-ST2 (IL33 receptor)-NFκB axis in granulosa in early atretic follicles may regulate those two events. Injection of hCG has been shown to induce a transient peak of IL33 expression with synchronized atresia. In this model, IL33-independent expression of ST2 in granulosa cells was detected in early atretic follicles before macrophage invasion...
January 25, 2024: Biology of Reproduction
https://read.qxmd.com/read/38267364/lrrc52-is-likely-a-functional-component-of-human-ksper
#33
JOURNAL ARTICLE
Jiali Zhang, Liping Zheng, Ying Chen, Tao Luo, Xuhui Zeng, Hang Kang
Completion of fertilization is orchestrated by various ion channels in sperm membrane. Hyperpolarization of membrane potential, an indispensable event during the capacitation process, is dominated by sperm potassium channel (KSper). In addition to sperm-specific SLO3, which forms the channel pore, the auxiliary subunit leucine-rich-repeat-containing protein 52 (LRRC52) is required to form mKSper to function under physiological conditions. However, in human sperm, although most evidence supports that hSLO3 is the pore-forming subunit, whether hLRRC52 contributes to hKSper conductance and modulates sperm function remains to be understood...
January 24, 2024: Biology of Reproduction
https://read.qxmd.com/read/38267362/role-of-mirnas-in-glucose-metabolism-of-mouse-cumulus-cells
#34
JOURNAL ARTICLE
Rui-Jie Ma, Min Zhang, Jia-Shun Wu, Zhi-Peng Wang, Guo-Liang Wang, Nan He, Ming-Jiu Luo, Jing-He Tan
It is known that the oocyte has a limited capacity to acquire and metabolize glucose, and it must rely on cumulus cells (CCs) to take up glucose and produce pyruvate for use to produce ATP through oxidative phosphorylation. We therefore propose that miRNAs might regulate glucose metabolism (GM) in CCs and might be used as markers for oocyte quality assessment. Here, mouse CC models with impaired glycolysis or pentose phosphate pathway (PPP) were established, and miRNAs targeting the key enzymes in glycolysis/PPP were predicted using the miRNA target prediction databases...
January 24, 2024: Biology of Reproduction
https://read.qxmd.com/read/38263524/fgf2-lif-and-igf-1-fli-supplementation-improves-mouse-oocyte-in-vitro-maturation-via-increased-glucose-metabolism
#35
JOURNAL ARTICLE
Asrafun Nahar, John Becker, Rolando Pasquariello, Jason Herrick, Heather Rogers, Mingxiang Zhang, William Schoolcraft, Rebecca L Krisher, Ye Yuan
Chemically defined oocyte maturation media supplemented with FGF2, LIF and IGF-1 (FLI medium) enabled significantly improved oocyte quality in multiple farm animals, yet the molecular mechanisms behind such benefits were poorly defined. Here, we first demonstrated that FLI medium enhanced mouse oocyte quality assessed by blastocyst formation after in vitro fertilization, and implantation and fetal development after embryo transfer. We then analyzed the glucose concentrations in the spent media, ROS concentrations, mitochondrial membrane potential, spindle morphology in oocytes, and the abundance of transcripts of EGF-like factors, cumulus expansion factors, and glucose metabolism related genes in cumulus cells...
January 23, 2024: Biology of Reproduction
https://read.qxmd.com/read/38236177/coiled-coil-domain-containing-159-ccdc159-is-required-for-spermatid-head-and-tail-assembly-in-mice
#36
JOURNAL ARTICLE
Tingting Ge, Lu Yuan, Linwei Xu, Fan Yang, Wenhua Xu, Changmin Niu, Guanghua Li, Huiping Zhou, Ying Zheng
The centrosome is critical for maintaining the sperm head-tail connection and the formation of flagellar microtubules. In this study, we found that in mouse testes, coiled-coil domain-containing protein 159 (CCDC159) is specifically localized to the head-tail coupling apparatus (HTCA) of spermatids, a structure that ensures sperm head-tail tight conjunction. CCDC159 contains a C-terminal coiled-coil domain that functions as the centrosomal localization signal. Gene knockout (KO) of Ccdc159 in mice resulted in acephalic spermatozoa, abnormal flagella, and male infertility...
January 17, 2024: Biology of Reproduction
https://read.qxmd.com/read/38224314/targeted-deletion-of-nr2f2-and-vcam1-in-theca-cells-impacts-ovarian-follicular-development-insights-into-pcos
#37
JOURNAL ARTICLE
Nicholes R Candelaria, JoAnne S Richards
Defining features of Polycystic Ovary Syndrome (PCOS) include elevated expression of steroidogenic genes, theca cell androgen biosynthesis and peripheral levels of androgens. In previous studies we identified vascular cell adhesion molecule 1 (VCAM1) as a selective androgen target gene in specific NR2F2/SF1 (+/+) theca cells. By deleting NR2F2 and VCAM1 selectively in CYP17A1 theca cells in mice, we documented that NR2F2 and VCAM1 impact distinct and sometimes opposing theca cell functions that alter ovarian follicular development in vivo: including major changes in ovarian morphology, steroidogenesis, gene expression profiles, immunolocalization images (NR5A1, CYP11A1, NOTCH1, CYP17A1, INSL3, VCAM1, NR2F2) as well as granulosa cell functions...
January 15, 2024: Biology of Reproduction
https://read.qxmd.com/read/38224305/roles-of-histone-post-translational-modifications-in-meiosis
#38
JOURNAL ARTICLE
Hui Nie, Xueyu Kong, Xiaoyu Song, Xiaoyu Guo, Zhanyu Li, Cunxian Fan, Binyuan Zhai, Xiao Yang, Ying Wang
Histones post-translational modifications (PTMs), such as phosphorylation, methylation, acetylation and ubiquitination, play vital roles in various chromatin-based cellular processes. Meiosis is crucial for organisms that depend on sexual reproduction to produce haploid gametes, during which chromatin undergoes intricate conformational changes. An increasing body of evidence is clarifying the essential roles of histone PTMs during meiotic divisions. In this review, we concentrate on the PTMs of H2A, H2B, H3 and H4 as well as the linker histone H1 that are required for meiosis and summarize recent progress in understanding how these modifications influence diverse meiotic events...
January 15, 2024: Biology of Reproduction
https://read.qxmd.com/read/38224118/correction-to-the-fluid-factor-ovgp1-provides-a-significant-oviductal-microenvironment-for-the-reproductive-process-in-golden-hamster
#39
(no author information available yet)
No abstract text is available yet for this article.
January 15, 2024: Biology of Reproduction
https://read.qxmd.com/read/38217862/multiple-genes-in-the-pate5-13-genomic-region-contribute-to-adam3-processing
#40
JOURNAL ARTICLE
Taichi Noda, Hina Shinohara, Sumire Kobayashi, Ayumu Taira, Seiya Oura, Duritahala, Midori Tokuyasu, Kimi Araki, Masahito Ikawa
Sperm proteins undergo post-translational modifications during sperm transit through the epididymis to acquire fertilizing ability. We previously reported that the genomic region coding Pate family genes is key to the proteolytic processing of the sperm membrane protein ADAM3 and male fertility. This region contains nine Pate family genes (Pate5-13), and two protein-coding genes (Gm27235 and Gm5916), with a domain structure similar to Pate family genes. Therefore, in this study, we aimed to identify key factors by narrowing the genomic region...
January 13, 2024: Biology of Reproduction
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