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Journal of Microscopy

Mikhail V Svistushkin, Svetlana L Kotova, Anatoly B Shekhter, Valery M Svistushkin, Anastasia A Akovantseva, Anastasia A Frolova, Alexey L Fayzullin, Svetlana V Starostina, Evgeny A Bezrukov, Roman B Sukhanov, Sergey F Timashev, Denis V Butnaru, Peter S Timashev
Regenerative medicine opens new opportunities in the repair of cicatricial lesions of the vocal folds. Here, we present a thorough morphological study, with the focus on the collagen structures in the mucosa of the vocal folds, dedicated to the effects of stem cells on the vocal folds repair after cicatricial lesions. We used a conventional experimental model of a mature scar of the rabbit vocal folds, which was surgically excised with a simultaneous implantation of autologous bone marrow-derived mesenchymal stem cells (MSC) into the defect...
February 11, 2019: Journal of Microscopy
Zhiping Zeng, Jing Ma, Xuanze Chen, Canhua Xu
In this paper, we propose a promising super-resolution imaging scheme in fluorescence lifetime domain (lifetime super-resolution optical fluctuation imaging, ltSOFI). ltSOFI has the potential to obtain super-resolution images by taking advantage of fluorescence lifetime blinking under wide-field lifetime detection. The proof-of-concept for ltSOFI was demonstrated through numerical simulation of high-order cumulant analysis on fluorescence lifetime blinking emitters. As a tentative experimental demonstration, we obtained super-resolution lifetime imaging from time-lapse FLIM recording of HeLa cells expressing a cAMP sensor using ltSOFI method...
February 8, 2019: Journal of Microscopy
Johan Ploem
The observation with RCM of the reflection from reaction products produced by non-isotopic in situ hybridization and a peroxidase staining, has recently facilitated the identification of single copy genes. RCM also reveals light microscope structures in stained ultrathin (0.1 micron) epon and lowicryl sections. In such preparations no out-of-focus blur can be observed. Confocal optics are thus not needed to obtain high-quality images of ultrathin sections at the highest conventional light microscope optical resolution, and with higher image-contrast than can be obtained by classical absorption microscopy...
February 5, 2019: Journal of Microscopy
J Ohser, J Lacayo-Pineda, M Putman, A Rack, D Dobrovolskij
A new microscopic principle based on radiometric stereo microscopy is presented which is designed for investigating macro-dispersion of filler in rubber. The image acquisition is combined with a stereological method of estimating the volume-weighted size distribution of the filler particles. Experimental results for carbon black filler in rubber obtained by radiometric stereo microscopy are compared with those from microtomography using synchrotron radiation and, furthermore, a simulation study is used for evaluation...
January 30, 2019: Journal of Microscopy
C Zhang, Y Liu, H Sun, F Lin, Y Ma, W Qu, T Chen
Acceptor-sensitized 3-cube fluorescence resonance energy transfer (FRET) imaging (also termed as E-FRET imaging) is a popular fluorescence intensity-based FRET quantification method. Here, an automated E-FRET microscope with user-friendly interfaces was set up for dynamical online quantitative live-cell FRET imaging. This microscope reduces the time of a quantitative E-FRET imaging from 12 to 3 seconds. After locating cells, calibration of the microscope and E-FRET imaging of the cells can be performed automatically by clicking "Capture" button on interfaces...
January 28, 2019: Journal of Microscopy
Stephane Perrin, Hongyu Li, Audrey Leong-Hoi, Sylvain Lecler, Paul Montgomery
White-light microsphere-assisted microscopy is a full-field and label-free imaging promising technique making it possible to achieve a sub-diffraction lateral resolution. However, performance of this technique depends not only on the geometrical parameters but also on the illumination conditions of the optical system. In the present work, experimental measurements and computer simulations have been performed in air in order to determine the influence of the two diaphragm apertures of the Köhler arrangement and the spectral width of the light source on both the depth-of-focus of the microsphere and the optimisation of the imaging contrast...
January 25, 2019: Journal of Microscopy
David Partouche, Jérémie Mathurin, Antoine Malabirade, Sergio Marco, Christophe Sandt, Véronique Arluison, Ariane Deniset-Besseau, Sylvain Trépout
Propagation of structural information through conformational changes in host-encoded amyloid proteins is at the root of many neurodegenerative disorders. Although important breakthroughs have been made in the field, fundamental issues like the 3D-structures of the fibrils involved in some of those disorders are still to be elucidated. To better characterise those nanometric fibrils, a broad range of techniques is currently available. Nevertheless none of them is able to perform direct chemical characterisation of single protein fibrils...
January 16, 2019: Journal of Microscopy
J J H A VAN Hest, A V Agronskaia, J Fokkema, F Montanarella, A Gregorio Puig, C DE Mello Donega, A Meijerink, G A Blab, H C Gerritsen
Fiducial markers are used in correlated light and electron microscopy (CLEM) to enable accurate overlaying of fluorescence and electron microscopy images. Currently used fiducial markers, e.g. dye-labelled nanoparticles and quantum dots, suffer from irreversible quenching of the luminescence after electron beam exposure. This limits their use in CLEM, since samples have to be studied with light microscopy before the sample can be studied with electron microscopy. Robust fiducial markers, i.e. luminescent labels that can (partially) withstand electron bombardment, are interesting because of the recent development of integrated CLEM microscopes...
January 16, 2019: Journal of Microscopy
Marzena Tkaczyk, Kalin I Dragnevski, Clive R Siviour, Gary Edwards
In this paper, we present the results from novel microscopy-based approaches aimed at providing further insight into the mechanism of film formation and associated mechanical response in polymer lattices. Firstly, a 'simple' methodology, combining the use of variable pressure scanning electron microscopy and a recently introduced enhanced coolstage (-50 to +50°C), was successfully developed and not only used to study dynamic processes, e.g. different stages of latex film formation, but also for high-resolution imaging of 'freeze-dried' structures...
January 4, 2019: Journal of Microscopy
N Cautaerts, R Delville, D Schryvers
This work describes the concepts, the mathematical framework and the implementation of the open source ALPHABETA software, a dedicated tool for calculating α and β tilt angles on any TEM double tilt stage. ALPHABETA provides an intuitive user interface to calibrate the orientation of the region of interest and to subsequently calculate the angle tilts to reach a zone axis, to perform a rotation around a given reflection, or to set up a 2-beam or weak beam condition. The orientation of the sample on the stage and the tilt range reachable domain can be visualised in a stereographic projection plot...
December 24, 2018: Journal of Microscopy
C Barcellini, S Dumbill, E Jimenez-Melero
We have performed an in-depth characterisation of the microstructure evolution of 20Cr-25Ni Nb-stabilised austenitic stainless steel during 1 h isochronal annealing up to 1100°C using scanning electron microscopy. This steel grade is used as cladding material in Advanced Gas-cooled fission reactors, due to its resistance to thermal creep and oxidation. The initial deformed microstructure undergoes recrystallisation via a strain-induced boundary migration mechanism, attaining a fully recrystallised microstructure at 850°C...
December 17, 2018: Journal of Microscopy
P Purwar, S Han, Y Lee, B Saha, T Sandhan, J Lee
Commercial high-resolution optical microscopes are essential for microscopy imaging; however, they are expensive and bulky, which limits their use in point-of-care devices, resource-limited areas, and real-time imaging of a sample in a large apparatus. In this study, we report a novel compact (10 cm × 5 cm × 5 cm, without the light source) lightweight (∼0.5 kg) submicron-resolution inverted optical microscope at low cost (∼$ 300). Our technique utilises the proximity of the image sensor to a commercial microscope objective lens for compactness of the microscope...
December 17, 2018: Journal of Microscopy
I Munro, E García, M Yan, S Guldbrand, S Kumar, K Kwakwa, C Dunsby, M A A Neil, P M W French
Super-resolved microscopy techniques have revolutionized the ability to study biological structures below the diffraction limit. Single molecule localization microscopy (SMLM) techniques are widely used because they are relatively straightforward to implement and can be realized at relatively low cost, e.g. compared to laser scanning microscopy techniques. However, while the data analysis can be readily undertaken using open source or other software tools, large SMLM data volumes and the complexity of the algorithms used often lead to long image data processing times that can hinder the iterative optimization of experiments...
December 3, 2018: Journal of Microscopy
L Kou, L Jin, H Lei, C Hu, H Li, X Hu, X Hu
Real-time tracking of multiple particles is key for quantitative analysis of dynamic biophysical processes and materials science via time-lapse microscopy image data, especially for single molecule biophysical techniques, such as magnetic tweezers and centrifugal force microscopy. However, real-time multiple particle tracking with high resolution is limited by the current imaging processes or tracking algorithms. Here, we demonstrate 1 nm resolution in three dimensions in real-time with a graphics-processing unit (GPU) based on a compute unified device architecture (CUDA) parallel computing framework instead of only a central processing unit (CPU)...
November 29, 2018: Journal of Microscopy
A Nicolaÿ, J M Franchet, J Cormier, H Mansour, M DE Graef, A Seret, N Bozzolo
The importance of angular resolution in EBSD analyses is discussed based on an Inconel 718 sample containing several populations of recrystallized grains, with subtle differences in dislocation contents. Classical EBSD analyses (with angular resolution in the range of 0.5-1°) do not allow for distinguishing recrystallized grains grown dynamically or post-dynamically. The angular resolution of EBSD orientation and misorientation data can be significantly improved (down to about 0.1-0.2°) either using more sophisticated Kikuchi pattern indexing methods and/or using the recently proposed LLASS denoising filter (Local Linear Automatic Smoothing Splines)...
November 26, 2018: Journal of Microscopy
(no author information available yet)
No abstract text is available yet for this article.
March 2019: Journal of Microscopy
(no author information available yet)
No abstract text is available yet for this article.
December 2018: Journal of Microscopy
G Tozzi, F Ahmed
No abstract text is available yet for this article.
December 2018: Journal of Microscopy
L Vojtová, T Zikmund, V Pavliňáková, J Šalplachta, D Kalasová, E Prosecká, J Brtníková, J Žídek, D Pavliňák, J Kaiser
This study presents an X-ray computed nanotomography (nano-CT) based, high-resolution imaging technique. Thanks to a voxel resolution of 540 nm, this novel technique is suitable for observing the 3D morphology of soft biopolymeric scaffolds seeded with stem cells. A sample of highly porous collagen scaffold seeded with contrasted mesenchymal stem cells (MSC) was investigated by using lab-based nano-CT. The whole volume of the sample was analysed without its destruction. To evaluate the potential of nano-CT, a comparison measurement was done using a standard microscopy technique...
November 22, 2018: Journal of Microscopy
K Losch, S Schuff, F Balle, T Beck, C Redenbach
Metal matrix composites are complex materials consisting of various phases which can display largely different mechanical properties. The deformation behaviour of these composites cannot be sufficiently modelled by averages or simple particle shapes due to the local stresses that occur on the particle edges. Therefore, a sophisticated model of the microstructure is needed. We introduce a method for stochastic modelling of a silicon carbide (SiC) particle reinforced aluminium matrix composite. The SiC particles are modelled by Laguerre polyhedra generated by densely packed spheres...
November 16, 2018: Journal of Microscopy
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