Journals Pflügers Archiv : European ...

Pflügers Archiv : European Journal of Physiology
Christian Stock
No abstract text is available yet for this article.
May 8, 2024: Pflügers Archiv: European Journal of Physiology
Tina Schönberger, Marie Jakobs, Anna-Lena Friedel, Tina Hörbelt-Grünheidt, Bastian Tebbe, Oliver Witzke, Manfred Schedlowski, Joachim Fandrey
Cells of the immune defence, especially leukocytes, often have to perform their function in tissue areas that are characterized by oxygen deficiency, so-called hypoxia. Physiological hypoxia significantly affects leukocyte function and controls the innate and adaptive immune response mainly through transcriptional gene regulation via the hypoxia-inducible factors (HIFs). Multiple pathogens including components of bacteria, such as lipopolysaccharides (LPS) trigger the activation of leukocytes. HIF pathway activation enables immune cells to adapt to both hypoxic environments in physiological and inflammatory settings and modulates immune cell responses through metabolism changes and crosstalk with other immune-relevant signalling pathways...
May 7, 2024: Pflügers Archiv: European Journal of Physiology
Jonna Airaksinen, Satu Siimes, Juha Hartikainen, Seppo Ylä-Herttuala
Arrhythmia detection is essential when assessing the safety of novel drugs and therapies in preclinical studies. Many short-term arrhythmia monitoring methods exist, including non-invasive ECG and Holter. However, there are no reliable, long-term, non-invasive, or minimally invasive methods for cardiac arrhythmia follow-up in large animals that allows free movement with littermates. A long follow-up time is needed when estimating the impact of long-lasting drugs or therapies, such as gene therapy. We evaluated the feasibility and performance of insertable cardiac monitors (ICMs) in pigs for minimally invasive, long-term monitoring of cardiac arrhythmias that allows free movement and species-specific behavior...
May 4, 2024: Pflügers Archiv: European Journal of Physiology
Nermeen Bastawy, Aliaa E M K El-Mosallamy, Samira H Aljuaydi, Huda O AbuBakr, Rabab Ahmed Rasheed, A S Sadek, R T Khattab, Wael Botros Abualyamin, Shereen E Abdelaal, Amy F Boushra
Hyperthyroidism-induced cardiac disease is an evolving health, economic, and social problem affecting well-being. Sodium-glucose cotransporter protein 2 inhibitors (SGLT2-I) have been proven to be cardio-protective when administered in cases of heart failure. This study intended to investigate the potential therapeutic effect of SGLT2-I on hyperthyroidism-related cardiopulmonary injury, targeting the possible underlying mechanisms. The impact of the SGLT2-I, dapagliflozin (DAPA), (1 mg/kg/day, p.o) on LT4 (0...
May 3, 2024: Pflügers Archiv: European Journal of Physiology
Aline Cristina Parletta, Gabriela Cavazza Cerri, Claudia Ribeiro Borba Gasparini, Karine Panico, Denival Nascimento Vieira-Junior, Larissa Maria Zacarias-Rodrigues, Nathalia Senger, Amanda de Almeida Silva, Marina Fevereiro, Gabriela Placoná Diniz, Maria Cláudia Costa Irigoyen, Maria Luiza Morais Barreto-Chaves
Cardiac hypertrophy (CH) is an adaptive response to maintain cardiac function; however, persistent stress responses lead to contractile dysfunction and heart failure. Although inflammation is involved in these processes, the mechanisms that control cardiac inflammation and hypertrophy still need to be clarified. The NLRP3 inflammasome is a cytosolic multiprotein complex that mediates IL-1β production. The priming step of NLRP3 is essential for increasing the expression of its components and occurs following NF-κB activation...
April 29, 2024: Pflügers Archiv: European Journal of Physiology
Leonie Inderwiedenstraße, Marie-Cécile Kienitz
Signaling of G protein-activated inwardly rectifying K+ (GIRK) channels is an important mechanism of the parasympathetic regulation of the heart rate and cardiac excitability. GIRK channels are inhibited during stimulation of Gq -coupled receptors (Gq PCRs) by depletion of phosphatidyl-4,5-bisphosphate (PIP2 ) and/or channel phosphorylation by protein kinase C (PKC). The Gq PCR-dependent modulation of GIRK currents in terms of specific PKC isoform activation was analyzed in voltage-clamp experiments in rat atrial myocytes and in CHO or HEK 293 cells...
April 24, 2024: Pflügers Archiv: European Journal of Physiology
Megan A Palmer, Yvonne Benatzy, Bernhard Brüne
Human arachidonate 15-lipoxygenase type B is a lipoxygenase that catalyzes the peroxidation of arachidonic acid at carbon-15. The corresponding murine ortholog however has 8-lipoxygenase activity. Both enzymes oxygenate polyunsaturated fatty acids in S-chirality with singular reaction specificity, although they generate a different product pattern. Furthermore, while both enzymes utilize both esterified fatty acids and fatty acid hydro(pero)xides as substrates, they differ with respect to the orientation of the fatty acid in their substrate-binding pocket...
April 19, 2024: Pflügers Archiv: European Journal of Physiology
Nadine Merz, Jennifer Christina Hartel, Sabine Grösch
The integrity of the colon and the development of colon cancer depend on the sphingolipid balance in colon epithelial cells. In this review, we summarize the current knowledge on how ceramides and their complex derivatives influence normal colon development and colon cancer development. Ceramides, glucosylceramides and sphingomyelin are essential membrane components and, due to their biophysical properties, can influence the activation of membrane proteins, affecting protein-protein interactions and downstream signalling pathways...
April 18, 2024: Pflügers Archiv: European Journal of Physiology
Sandy MacMillan, David P Burns, Ken D O'Halloran, A Mark Evans
Functional magnetic resonance imaging (fMRI) suggests that the hypoxic ventilatory response is facilitated by the AMP-activated protein kinase (AMPK), not at the carotid bodies, but within a subnucleus (Bregma -7.5 to -7.1 mm) of the nucleus tractus solitarius that exhibits right-sided bilateral asymmetry. Here, we map this subnucleus using cFos expression as a surrogate for neuronal activation and mice in which the genes encoding the AMPK-α1 (Prkaa1) and AMPK-α2 (Prkaa2) catalytic subunits were deleted in catecholaminergic cells by Cre expression via the tyrosine hydroxylase promoter...
April 18, 2024: Pflügers Archiv: European Journal of Physiology
Karolos-Philippos Pissas, Stefan Gründer, Yuemin Tian
Fast growing solid tumors are frequently surrounded by an acidic microenvironment. Tumor cells employ a variety of mechanisms to survive and proliferate under these harsh conditions. In that regard, acid-sensitive membrane receptors constitute a particularly interesting target, since they can affect cellular functions through ion flow and second messenger cascades. Our knowledge of these processes remains sparse, however, especially regarding medulloblastoma, the most common pediatric CNS malignancy. In this study, using RT-qPCR, whole-cell patch clamp, and Ca2+ -imaging, we uncovered several ion channels and a G protein-coupled receptor, which were regulated directly or indirectly by low extracellular pH in DAOY and UW228 medulloblastoma cells...
April 16, 2024: Pflügers Archiv: European Journal of Physiology
Joshua Godoy Coto, Erica V Pereyra, Fiorella A Cavalli, Carlos A Valverde, Claudia I Caldiz, Sabina M Maté, Alejandra M Yeves, Irene L Ennis
The myocardium is a highly oxidative tissue in which mitochondria are essential to supply the energy required to maintain pump function. When pathological hypertrophy develops, energy consumption augments and jeopardizes mitochondrial capacity. We explored the cardiac consequences of chronic swimming training, focusing on the mitochondrial network, in spontaneously hypertensive rats (SHR). Male adult SHR were randomized to sedentary or trained (T: 8-week swimming protocol). Blood pressure and echocardiograms were recorded, and hearts were removed at the end of the training period to perform molecular, imaging, or isolated mitochondria studies...
April 16, 2024: Pflügers Archiv: European Journal of Physiology
Andreas Draguhn, Armin Kurtz
No abstract text is available yet for this article.
April 16, 2024: Pflügers Archiv: European Journal of Physiology
Stina Becker, Aljoscha Swoboda, Henrik Siemer, Sandra Schimmelpfennig, Sarah Sargin, Victor Shahin, Albrecht Schwab, Karolina Najder
Neutrophil granulocytes play a crucial role in host defense against invading pathogens and in inflammatory diseases. The aim of this study was to elucidate membrane potential dynamics during the initial phase of neutrophil activation and its relation to migration and production of reactive oxygen species (ROS). We performed ROS production measurements of neutrophils from healthy C57BL/6J mice after TNFα-priming and/or C5a stimulation. The actin cytoskeleton was visualized with fluorescence microscopy...
April 13, 2024: Pflügers Archiv: European Journal of Physiology
Josef Pfeilschifter, Erich Gulbins
No abstract text is available yet for this article.
April 9, 2024: Pflügers Archiv: European Journal of Physiology
A Michelucci, L Catacuzzeno
All animal cells control their volume through a complex set of mechanisms, both to counteract osmotic perturbations of the environment and to enable numerous vital biological processes, such as proliferation, apoptosis, and migration. The ability of cells to adjust their volume depends on the activity of ion channels and transporters which, by moving K+ , Na+ , and Cl- ions across the plasma membrane, generate the osmotic gradient that drives water in and out of the cell. In 2010, Patapoutian's group identified a small family of evolutionarily conserved, Ca2+ -permeable mechanosensitive channels, Piezo1 and Piezo2, as essential components of the mechanically activated current that mediates mechanotransduction in vertebrates...
April 6, 2024: Pflügers Archiv: European Journal of Physiology
Hang Lam Li, Arthur Verhoeven, Ronald Oude Elferink
Soluble adenylyl cyclase (sAC) differs from transmembrane adenylyl cyclases (tmAC) in many aspects. In particular, the activity of sAC is not regulated by G-proteins but by the prevailing bicarbonate concentrations inside cells. Therefore, sAC serves as an exquisite intracellular pH sensor, with the capacity to translate pH changes into the regulation of localization and/or activity of cellular proteins involved in pH homeostasis. In this review, we provide an overview of literature describing the regulation of sAC activity by bicarbonate, pinpointing the importance of compartmentalization of intracellular cAMP signaling cascades...
April 6, 2024: Pflügers Archiv: European Journal of Physiology
Hoda M Moghazy, Nesreen G Abdelhaliem, Sherine Ahmed Mohammed, Asmaa Hassan, Amany Abdelrahman
No abstract text is available yet for this article.
April 5, 2024: Pflügers Archiv: European Journal of Physiology
Ivan-Maximiliano Kur, Andreas Weigert
Cancer is the second leading cause of mortality worldwide. Despite recent advances in cancer treatment including immunotherapy with immune checkpoint inhibitors, new unconventional biomarkers and targets for the detection, prognosis, and treatment of cancer are still in high demand. Tumor cells are characterized by mutations that allow their unlimited growth, program their local microenvironment to support tumor growth, and spread towards distant sites. While a major focus has been on altered tumor genomes and proteomes, crucial signaling molecules such as lipids have been underappreciated...
April 4, 2024: Pflügers Archiv: European Journal of Physiology
Emily DeMichele, Andre G Buret, Cormac T Taylor
Mammalian cells utilize glucose as a primary carbon source to produce energy for most cellular functions. However, the bioenergetic homeostasis of cells can be perturbed by environmental alterations, such as changes in oxygen levels which can be associated with bacterial infection. Reduction in oxygen availability leads to a state of hypoxia, inducing numerous cellular responses that aim to combat this stress. Importantly, hypoxia strongly augments cellular glycolysis in most cell types to compensate for the loss of aerobic respiration...
April 4, 2024: Pflügers Archiv: European Journal of Physiology
Kathrin Skoczynski, Andre Kraus, Christoph Daniel, Maike Büttner-Herold, Kerstin Amann, Mario Schiffer, Kristina Hermann, Leonie Herrnberger-Eimer, Ernst R Tamm, Bjoern Buchholz
Complex interactions of the branching ureteric bud (UB) and surrounding mesenchymal cells during metanephric kidney development determine the final number of nephrons. Impaired nephron endowment predisposes to arterial hypertension and chronic kidney disease. In the kidney, extracellular matrix (ECM) proteins are usually regarded as acellular scaffolds or as the common histological end-point of chronic kidney diseases. Since only little is known about their physiological role in kidney development, we aimed for analyzing the expression and role of fibronectin...
April 2, 2024: Pflügers Archiv: European Journal of Physiology
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