Luisa Agnello, Matteo Vidali, Giuseppe Salvaggio, Francesco Agnello, Bruna Lo Sasso, Caterina Maria Gambino, Marcello Ciaccio
INTRODUCTION: The aim of this study is to assess the usefulness of the Prostate Health Index (PHI) as a triage tool for selecting patients at risk of prostate cancer (PCa) who should undergo multiparametric Magnetic Resonance Imaging (MRI). MATERIAL AND METHODS: We enrolled 204 patients with suspected PCa. For each patient, a blood sample was collected before mpMRI to measure PHI. Findings on mpMRI were assessed according to the Prostate Imaging Reporting & Data System version 2...
April 5, 2024: Clinical Biochemistry
Qian Zhang, Huiyi Chen, Meng Wang, Huiying Lai, Wensong Liu, Lijuan Wang, Jiaqi Zhang, Chuanbao Li, Weiyan Zhou
BACKGROUND: This study aims to investigate the impact of age and sex on high-sensitivity cardiac troponin T (hs-cTnT) and establish 99th percentile upper reference limits (URLs) in older individuals utilizing large-scale real-world data. METHODS: 40,530 outpatient hs-cTnT results were obtained from the laboratory database from January 1, 2018, to December 31, 2023. Our study included 4,199 elderly outpatients (aged ≥60) without cardiovascular disease or other heart-related chronic conditions...
April 4, 2024: Clinical Biochemistry
Adam Jimenez, Ashley Rose Scholl, Bangchen Wang, Michael Schilke, Eric D Carlsen
OBJECTIVES: Some therapeutic monoclonal antibodies, like daratumumab and elotuzumab, produce interfering monoclonal bands on serum protein electrophoresis (SPEP) and immunofixation electrophoresis (IFE). Whether other common therapeutic antibodies also produce interference has not been systematically evaluated. DESIGN AND METHODS: SPEP/IFE from patients receiving isatuximab (48 patients), belantamab mafodotin (BM; 41), and denosumab (41) were retrospectively reviewed for therapeutic antibody interference...
March 31, 2024: Clinical Biochemistry
Ahmet Kor, Serdar Can Güven, Selçuk Akan, Funda Eren, Hatice Ecem Konak, Yüksel Maraş, Kevser Orhan, Salim Neşelioğlu, Şükran Erten
BACKGROUND: Recent data show that netrin-1 has a role in development of pulmonary fibrosis. This study was aimed to investigate serum netrin-1 level and its relation to interstitial lung disease(ILD) in patients with rheumatoid arthritis (RA). METHOD: 42 RA patients with RA-ILD, 58 RA patients without RA-ILD (RA non-ILD group), and 61 healthy volunteers were included in this study. The modified DAS28-ESR score was used to calculate disease activity in RA patients...
March 29, 2024: Clinical Biochemistry
Karol P Steckiewicz, Magdalena A Wujtewicz, Michał Okrągły, Aleksander Aszkiełowicz, Malgorzata Dąbrowska, Radosław Owczuk
INTRODUCTION: Infection complications are common in intensive care unit patients, and early detection remains a diagnostic challenge. Procalcitonin (PCT) and C-reactive protein (CRP) are commonly used biomarkers. A novel diagnostic approach focuses on the host immune response. One of the novel approaches, the MMBV index, is based on measuring in a blood sample three parameters: (i) tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), (ii) interferon-γ-induced protein-10 (IP10), and (iii) CRP...
March 13, 2024: Clinical Biochemistry
Kwabena A N Sarpong, Su Hee Kim, Christopher R McCartney, Joseph R Wiencek, Lindsay A L Bazydlo
BACKGROUND: Immunoassays are important for routine clinical testing and medical diagnosis. However, they are limited by cross-reactivity especially at low analyte concentrations. There is a critical need to investigate compounds that can interfere with immunoassays. Herein, we describe the identification of canrenone, a spironolactone metabolite that falsely increases progesterone concentrations on the Abbott Architect i2000 Immunoassay. METHODS: Serum samples and assay diluents spiked with spironolactone or canrenone and progesterone concentrations were measured on the Architect i2000 and Immulite XPi immunoassay platforms...
March 12, 2024: Clinical Biochemistry
Kayla Moehnke, Jennifer Kemp, Michelle R Campbell, Ravinder J Singh, Anne E Tebo, Anthony Maus
INTRODUCTION: 2,3-dinor 11β-Prostaglandin F2α (BPG) is an arachidonic acid derivative and the most abundant metabolic byproduct of prostaglandin D2, which is released during mast cell activation. Therefore, measurements of BPG in urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS) provide a noninvasive method for evaluation and management of mast cell disorders. Measurements obtained by LC-MS/MS exhibit a high prevalence of chromatographic interferences resulting in challenges with optimal determination of BGP...
March 8, 2024: Clinical Biochemistry
Michael Gilligan, Christopher McGuigan, Andrew McKeon
A rapidly expanding repertoire of neural antibody biomarkers exists for autoimmune central nervous system disorders. Following clinical recognition of an autoimmune CNS disorder, the detection of a neural antibody facilitates diagnosis and informs prognosis and management. This review considers the phenotypes, diagnostic assay methodologies, and clinical utility of neural antibodies in autoimmune CNS disorders. Autoimmune CNS disorders may present with a diverse range of clinical features. Clinical phenotype should inform the neural antibodies selected for testing via the use of phenotype-specific panels...
March 8, 2024: Clinical Biochemistry
Urszula Lechowicz, Beatriz Martinez-Delgado, Bin Liu, Sabine Wrenger, Adriana Rozy, Aneta Zdral, David S DeLuca, Tobias Welte, Sabina Janciauskiene, Joanna Chorostowska-Wynimko
INTRODUCTION: Compared to normal PiMM, individuals with severe α1-antitrypsin (AAT) PiZZ (Glu342Lys) genotype deficiency are at higher risk of developing early-onset chronic obstructive pulmonary disease (COPD)/emphysema associated with Z-AAT polymers and neutrophilic inflammation. We aimed to investigate putative differences in plasma levels of acute phase proteins (APP) between PiMM and PiZZ subjects and to determine plasma Z-AAT polymer levels in PiZZ subjects. MATERIALS AND METHODS: Nephelometric analysis of seven plasma APPs was performed in 67 PiMM and 44 PiZZ subjects, of whom 43 and 42, respectively, had stable COPD...
February 28, 2024: Clinical Biochemistry
Ji Liu, Wenpeng Ni, Kunyi Deng, Yanhui Chen, Guanghong Gu
BACKGROUND: Multiple Acyl-CoA Dehydrogenase Deficiency (MADD), also known as Glutaric Aciduria Type II, is an exceptionally rare autosomal recessive genetic disorder that disrupts the metabolism of fatty acids, amino acids, and choline. It presents with a wide range of clinical manifestations, from severe neonatal-onset forms to milder late-onset cases, with symptoms including metabolic disturbances and muscle weakness. Jordan's anomaly is a distinctive morphological feature found in peripheral blood white cells and is typically associated with Neutral Lipid Storage Disease (NLSD)...
February 22, 2024: Clinical Biochemistry
Abeer A Alrefai, Mai A H Abouelenin, Maha M A Salman, Gehan A E Tawfeek, Mona A Abbas
OBJECTIVES: Growing evidence suggests that systemic lupus erythematosus (SLE), an organ-damaging systemic autoimmune illness, may be influenced by long-noncoding RNAs (lncRNAs). This study aimed to assess the relative expression of lncRNAs (MIR31HG, NKILA, and PACER) in patients with SLE to evaluate their role in the disease. DESIGN AND METHODS: This study involved 70 patients with SLE and 70 apparently healthy control subjects. The expression levels of lnc-MIR31HG, NKILA, and PACER were quantified using real-time PCR...
February 21, 2024: Clinical Biochemistry
Maria Salinas, Emilio Flores, Alvaro Blasco, Maite Lopez-Garrigós, Ruth Torreblanca, María Leiva-Salinas, Irene Gutierrez, Carlos Leiva-Salinas
OBJECTIVES: Our objective was to shorten the screen for multiple myeloma (MM), through reflex testing. DESIGN AND METHODS: The clinical laboratory in the public University Hospital of San Juan (Alicante, Spain), serves 234,551 inhabitants. Through an intervention agreed with general practitioners, the Laboratory Information System (LIS) automatically registered serum immunoglobulins (Ig) when serum total proteins (STP) > 80 g/L for the first time in primary care patients...
February 20, 2024: Clinical Biochemistry
Yuhan Dong, Bo Long, Zhanglin Tian, Junmeng Huang, Youdong Wei
BACKGROUND: Recently acquired data suggests that sodium-glucose cotransporter-2 (SGLT2) may be a therapeutic target for cerebral ischemia. The specific impact of SGLT2 in acute ischemic stroke (AIS) remains unknown. We aimed to explore the levels of SGLT2 in AIS patients and its association with functional prognosis. METHODS: In this study, 132 AIS patients and 44 healthy controls were recruited prospectively to determine serum SGLT2 levels. Logistic regression analysis was employed to assess the association between serum SGLT2 level and stroke risk as well as 3-month outcome...
February 17, 2024: Clinical Biochemistry
Ronstan Lobo, Laura De Michieli, Grant M Spears, Elitza S Theel, Leslie J Donato, Amy M Wockenfus, Brandon R Kelley, Allan S Jaffe
INTRODUCTION: Multiple studies have investigated the role of cardiac troponin (cTn) in the risk stratification of patients with COVID-19. Most of these investigations are based on cTn values at presentation and do not consider the prognostic significance of cTn changes over time. This study aimed to investigate the prognostic role of serial cTn measurements in patients hospitalized with COVID-19 with samples that were not obtained for clinical indications. METHODS: Patients hospitalized between April 2020 and March 2021 with PCR-confirmed SARS-CoV-2 infection were evaluated...
February 14, 2024: Clinical Biochemistry
Peter A Kavsak, Lorna Clark, Saranya Arnoldo, Amy Lou, Jennifer L Shea, Shaun Eintracht, Andrew W Lyon, Vipin Bhayana, Laurel Thorlacius, Joshua E Raizman, Albert Tsui, Rose Djiana, Michael Chen, Yun Huang, Ali Haider, Ronald A Booth, Chris McCudden, Paul M Yip, Daniel Beriault, David Blank, Angela W S Fung, Jennifer Taher, Julie St-Cyr, Sameer Sharif, Emilie Belley-Cote, Beth L Abramson, Steven M Friedman, Jafna L Cox, Marco L A Sivilotti, Annabel Chen-Tournoux, Jesse McLaren, Susanna Mak, Venkatesh Thiruganasambandamoorthy, Frank Scheuermeyer, Karin H Humphries, Andrew Worster, Dennis Ko, Kristin M Aakre, Nicholas L Mills, Allan S Jaffe
BACKGROUND: An analytical benchmark for high-sensitivity cardiac troponin (hs-cTn) assays is to achieve a coefficient of variation (CV) of ≤ 10.0 % at the 99th percentile upper reference limit (URL) used for the diagnosis of myocardial infarction. Few prospective multicenter studies have evaluated assay imprecision and none have determined precision at the female URL which is lower than the male URL for all cardiac troponin assays. METHODS: Human serum and plasma matrix samples were constructed to yield hs-cTn concentrations near the female URLs for the Abbott, Beckman, Roche, and Siemens hs-cTn assays...
February 13, 2024: Clinical Biochemistry
Marwa A Gaber, Omnia H M Omar, Abdel-Raheim M A Meki, Ahmed Y Nassar, Ayman K M Hassan, Marwan S Mahmoud
BACKGROUND: Proprotein convertase subtilisin/kexin type 9 (PCSK-9) is a circulating protein that plays an important role in lipid metabolism and is linked to inflammation, which has implications for atherosclerosis and its severe cardiac effects. We studied the potential association of the PCSK-9 gene single nucleotide polymorphism (SNP), Oxidized low-density lipoprotein receptor 1- (OLR-1), and caspase-3 serum levels with the risk and severity of premature coronary artery disease (PCAD)...
February 10, 2024: Clinical Biochemistry
Paola Galozzi, Laura Sciacovelli, Daniela Basso
OBJECTIVES: Healthcare has a considerable environmental impact, yet it has been largely overlooked. Clinical laboratories, in particular, consume significantly more energy and water per unit area compared to standard office buildings. It is crucial to raise awareness among laboratories about the significance of embracing eco-friendly practices. Numerous energy-saving measures do not incur additional costs but necessitate a shift in organizational culture and mindset. DESIGN & METHODS: This paper conducts a cost-benefit analysis of energy consumption at the Laboratory Medicine Unit of University Hospital of Padova, beginning with laboratory refrigerators and freezers...
February 7, 2024: Clinical Biochemistry
Alessia Cafaro, Manuela Stella, Alessio Mesini, Elio Castagnola, Giuliana Cangemi, Francesca Mattioli, Giammarco Baiardi
Vancomycin is a glycopeptide antibiotic that has been adopted in clinical practice to treat gram-positive infections for more than 70 years. Despite vancomycin's long history of therapeutic use, optimal dose adjustments and pharmacokinetic/pharmacodynamic (PK/PD) target attainment in children are still under debate. Therapeutic drug monitoring (TDM) has been widely integrated into pediatric clinical practice to maximize efficacy and safety of vancomycin treatment. Area under the curve (AUC)-guided TDM has been recently recommended instead of trough-only TDM to ensure PK/PD target attainment of AUC0-24h /minimal inhibitory concentration (MIC) > 400 to 600 and minimize acute kidney injury risk...
February 5, 2024: Clinical Biochemistry
Min-Zhi Zhang, Qi-Guang Shi, Xiao-Yi Xu, Yuan Qi, Lin-Jie Zhang, Ning Zhao, Jia Li, Li-Min Li, Li-Sha Sun, Li Yang
BACKGROUND: Increased cerebrospinal fluid (CSF) β2-microglobulin (β2-MG) values are attributed to immune activation, lymphoid cell turnover and release of tissue destruction in the central nervous system (CNS). We investigated plasma and CSF β2-MG levels in adult patients with viral encephalitis/meningitis and their correlations with clinical parameters. METHOD: CSF samples from 26 patients with viral encephalitis/meningitis were collected. Moreover, 24 CSF samples from patients with non-inflammatory neurological disorders (NIND) as controls were collected...
February 3, 2024: Clinical Biochemistry
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