Silvia Bérgamo, Jaume Trapé, Laura González-García, Carolina González-Fernández, Carme Vergara, Noelia de-la-Torre, Glòria Trujillo, Dolors Estivill, Marco Antonio Álvarez-González, Laia Bosch, Marta Otero-Viñas, Carmen Bergós, Silvia Catot, Domingo Ruiz-Hidalgo, Sandra Ros, Francesc Sant
BACKGROUND AND AIMS: Human epididymal protein 4 (HE4) may be a useful tool in the differential diagnosis of malignant ascites. The aim of this study was to evaluate the diagnostic utility of HE4 for detecting malignant ascites, taking into account the possible false positives identified with adenosine deaminase (ADA), C-reactive protein (CRP), % polynuclear cells (%PMN) and glomerular filtration rate (eGFR). METHODS: Concentrations of HE4, ADA, %PMN and CRP were determined in 114 samples of peritoneal fluid and creatinine in serum in order to calculate eGFR...
September 9, 2023: Clinical Biochemistry
J J Miller, M K Bohn, V Higgins, M Nichols, Z Mohammed-Ali, T Henderson, R Selvaratnam, L Sepiashvili, K Adeli
OBJECTIVES: Prominent physiological changes occurring throughout childhood and adolescence necessitate the consideration of age and sex in biomarker interpretation. Critical gaps exist in pediatric reference intervals (RIs) for specialized endocrine markers, despite expected influence of growth and development. The current study aimed to establish and/or verify RIs for six specialized endocrine markers on a specialized immunoassay system. METHODS: Samples were collected from healthy children and adolescents (5 to <19 years) and apparently healthy outpatients (0 to <5 years) as part of the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER)...
September 4, 2023: Clinical Biochemistry
Han-Dong Zhao, Ju-Jun Sun, Tong-Bo Yu, Hong-Li Liu
PURPOSES: We aimed to investigate the levels of CD4+ CD8+ double positive (DP) T cells in patients with various severities of hemorrhagic fever with renal syndrome (HFRS), and the predictive capacity of DP T cells for the severity of this disorder. METHODS: The levels of DP T cells in 213 patients and 48 healthy donors were measured by flow cytometry, as were the levels of CD4+ T cells, CD8+ T cells, B lymphocytes, and natural killer (NK) cells. In each type of HFRS patient, we tested the basic clinical reference values for leukocytes, platelets, creatinine (Cr), uric acid (UA), and urea, and the values for activated partial thromboplastin time, prothrombin time, and fibrinogen, using conventional methods...
August 29, 2023: Clinical Biochemistry
Samantha Logan, Rajeevan Selvaratnam
INTRODUCTION: The commonly utilized method for determining low-density lipoprotein-cholesterol (LDLc) is by Friedewald based estimation (FeLDLc). A new approach to better estimate LDLc has been proposed by Sampson et al. 2020, known as the Sampson/National Institutes of Health (NIH) estimation (NeLDLc), to overcome the limitations of FeLDLc. Non-high-density lipoprotein-cholesterol (Non-HDLc), has equivalent cut-offs to LDLc, established by the 2021 Canadian Cardiovascular Society (CCS) guideline...
August 15, 2023: Clinical Biochemistry
Lawrence de Koning, Dennis Orton, Isolde Seiden Long, Jessica Boyd, Mark Kellogg, Amid Abdullah, Christopher Naugler, Albert Tsui, Brenda Strange, Dory Glaser
BACKGROUND: Minimizing hemolysis during phlebotomy ensures accurate chemistry results and reduces specimen cancellations and recollections. We developed videos demonstrating best practices to reduce hemolysis and tested whether distribution to clinical nurse educators (CNEs) for provision to nursing staff affected the degree of specimen hemolysis in hospital inpatient units and outpatient clinics. METHODS: Videos of common blood collections demonstrating best practices to reduce hemolysis were filmed and distributed via email link to all hospital-based CNEs in Calgary, Alberta, Canada...
August 12, 2023: Clinical Biochemistry
Shensong Li, Danni Mu, Chaochao Ma, Yixicuomu, Zhaxiyangzong, Jinrong Pang, Mingjun Zhan, Zhijuan Liu, Qu Dan, Xinqi Cheng
BACKGROUND: Hypoxia leads to different concentrations of the bicarbonate buffer system in Tibetan people. Indirect methods were used to establish the reference interval (RI) for total carbon dioxide (tCO2) based on big data from the adult population of Tibet, a high-altitude area in Western China. METHODS: Anonymous tCO2 test data (n=442,714) were collected from the People's Hospital of the Tibet Autonomous Region from January, 2018, to December, 2021. Multiple linear regression and variance component analyses were performed to assess the effects of sex, age, and race on tCO2 levels...
August 10, 2023: Clinical Biochemistry
Mary Kathryn Bohn, Shannon Steele, Khosrow Adeli
BACKGROUND: Humoral response against SARS-CoV-2 is increasingly accepted as the central correlate of immune protection. Recent pediatric seroprevalence data are extremely limited. Significant knowledge gaps also exist in immune response to mRNA SARS-CoV-2 vaccination in children. As children demonstrate distinct response to naïve infection relative to adults, it is essential to investigate age-specific differences in seroprevalence and antibody response to SARS-CoV-2 vaccination...
August 5, 2023: Clinical Biochemistry
Xiuzhu Hou, Shanshan Liu, Jing Liu, Jiansuo Zhou, Yongming Liang, Liyan Cui
OBJECTIVES: We examined the blood concentrations of carbohydrate antigen 125- Thomsen-nouveau (CA125-Tn) and anti-Müllerian hormone (AMH) in epithelial ovarian cancer (EOC) patients to evaluate their potential diagnostic utility together with CA125, human epididymis protein 4 (HE4) and Risk of Malignancy Algorithm (ROMA). DESIGN & METHODS: 50 healthy subjects, 45 EOC patients, 22 patients with borderline ovarian tumors (BOT), 21 patients with benign ovarian tumor (BET) and 45 patients with chocolate cyst of ovary (CCO) were studied...
July 28, 2023: Clinical Biochemistry
Ping Tan, Dandan Li, Lu Chang, Jiping Shi, Yanxi Han, Rui Zhang, Jinming Li
OBJECTIVE: To evaluate the current situation of expanded noninvasive prenatal screening (NIPS) for copy number variations (CNVs) in laboratories in China, the National Center of Clinical Laboratories conducted an externalqualityassessment (EQA) program. METHODS: The EQA panel consisted of 12 artificial samples associated with different syndromes, which were mixed with maternal plasma collected from pregnant women and enzyme-digested cell-free DNA (cfDNA) from cell lines with different fetal fractions (FFs) ranging from 5% to 15%...
July 26, 2023: Clinical Biochemistry
Lina Ying, Luping Gong, Sicen Meng, Xiudi Wu, Mingcai Li, Yan Li
BACKGROUND: Imbalances in cytokine networks have been shown to be a possible cause of rheumatoid arthritis (RA). The interleukin (IL)-12 family is involved in the pathogenesis of autoimmune diseases including RA, while IL-39 is a newly discovered member of the IL-12 family, although its role in RA remains unclear. The purpose of the present study was to detect the expression of IL-39 in the sera of patients with RA and its relationship with RA activity. METHODS: We recruited 46 patients with RA and 35 healthy controls at Ningbo Sixth Hospital...
July 25, 2023: Clinical Biochemistry
Breanne Maloney, Sunju Park, Mycroft Sowizral, Isa Brackett, Roxana Moslehi, Wendy K Chung, Dorota Gruber, Amy Brower, Michele Lloyd-Puryear, Michele Caggana, Norma P Tavakoli
INTRODUCTION: Newborn screening for Duchenne muscular dystrophy can be performed via a first-tier creatine kinase-MM measurement followed by reflex testing to second-tier molecular analysis of the DMD gene. In order to establish appropriate cut-offs for the creatine kinase-MM screen, factors that influence creatine kinase-MM in newborns were investigated. MATERIALS AND METHODS: Creatine kinase-MM data from a consented pilot study in New York State were collected over a two-year period and combined with de-identified validation data and analyzed...
July 19, 2023: Clinical Biochemistry
Giammarco Baiardi, Alessia Cafaro, Manuela Stella, Michela Cameran Caviglia, Maria Gabriella Poeta, Giuliana Cangemi, Francesca Mattioli
Altered direct oral anticoagulant (DOAC) plasma levels can lead either to spontaneous hemorrhagic or thrombotic complications. We describe a case of suspected altered apixaban disposition in a patient with an upper gastrointestinal cancer resection treated with apixaban for non-valvular atrial fibrillation. Diagnosis of ischemic stroke for left hemiparesis was confirmed due to recent emergence of a hypodense area in the posterior capsular nucleus of ischemic reference in a context of binuclear capsular lacunar lesions...
July 12, 2023: Clinical Biochemistry
İsmail Doğan, Ahmet Kor, Serdar Can Güven, Esra Fırat Oğuz, Salih Başer, Ebru Atalar, Yüksel Maraş, Özcan Erel, Şükran Erten
INTRODUCTION: The aim of the study is to investigate serum levels of 14-3-3 η (ETA) protein in patients with gout and possible relations with joint damage. METHOD: This cross-sectional study included 43 gout patients and 30 control patients. RESULTS: Serum 14-3-3 η protein levels were significantly higher in gout patients (median [IQR], 3.1 [2.0] vs 2.2 [1.0], p=0.007). In subgroup analyses of gout patients, serum 14-3-3 η protein levels did not differ between patients with and without a flare, tophaceous disease, elevated CRP and serum uric acid levels and a history of chronic kidney disease; however, were significantly higher in the patients with erosions (Median [IQR], 4...
July 8, 2023: Clinical Biochemistry
Luis J Morales-García, Rosa M Lillo Rodríguez, María S Pacheco-Delgado
BACKGROUND: Serum free light chain (FLC) quantification is a diagnostic criterion for monoclonal gammopathy and its values in patients with renal impairment are different from those in healthy subjects. The aim of this study was to evaluate Freelite and Kloneus assays in these patients. METHODS: In this retrospective study, serum samples from 226 patients with chronic kidney disease (CKD) of stages 2-5 were measured with a Freelite assay on the Optilite system and with a Kloneus assay on the AU5800 system and compared with controls without renal impairment...
July 8, 2023: Clinical Biochemistry
Stéphanie Lemaire, Aude Jacquot, Damien Denimal
No abstract text is available yet for this article.
July 7, 2023: Clinical Biochemistry
Eun-Jung Cho, Jinyoung Hong, Jungwon Hyun, Woochang Lee, Hyun Soo Kim, Sail Chun, Won-Ki Min
BACKGROUND: Interstitial lung abnormalities (ILAs) are associated with the risk of progression to interstitial lung diseases (ILDs). Krebs von den Lungen 6 (KL-6) and surfactant protein (SP)-A have been used as biomarkers of ILDs. In this study, we evaluated the levels of these biomarkers and identified their clinical correlations in healthy individuals to assess their usefulness in the diagnosis of ILAs. METHODS: The patient samples were categorized into three groups: healthy, disease, and ILD groups...
July 4, 2023: Clinical Biochemistry
Gregory Morgan, Laurent Briollais, Marc Clausen, Selina Casalino, Chloe Mighton, Sunakshi Chowdhary, Erika Frangione, Chun Yiu Jordan Fung, Saranya Arnoldo, Erin Bearss, Alexandra Binnie, Bjug Borgundvaag, Marc Dagher, Luke Devine, Steven M Friedman, Zeeshan Khan, Allison McGeer, Shelley L McLeod, David Richardson, Seth Stern, Ahmed Taher, Iris Wong, Natasha Zarei, Yvonne Bombard, Jordan Lerner-Ellis, Jennifer Taher
OBJECTIVES: Concepts related to SARS-CoV-2 laboratory testing and result interpretation can be challenging to understand. A cross-sectional survey of COVID-19 positive adults residing in Ontario, Canada was conducted to explore how well people understand SARS-CoV-2 laboratory tests and their associated results. DESIGN AND METHODS: Participants were recruited through fliers or by prospective recruitment of outpatients and hospitalized inpatients with COVID-19. Enrolled participants included consenting adults with a positive SARS-CoV-2 polymerase chain reaction test result...
July 3, 2023: Clinical Biochemistry
Lee F Schroeder
OBJECTIVES: Recent reference interval studies of the serum free light chain (FLC) test using contemporary instruments display divergence with the diagnostic range generally adopted as the international standard. In this study, we perform a retrospective reference interval analysis with risk predictions for monoclonal gammopathy. METHODS: Retrospective laboratory and clinical data for 8,986 patients were included in the study. Reference intervals were generated against a set of inclusion/exclusion criteria for two time periods representing the use of different instruments...
June 30, 2023: Clinical Biochemistry
Shuting Ren, Dongping Cai, Li Xiao, Hongshi Shen, Chuanlu Ren
BACKGROUND AND AIMS: We aimed to elucidate a hereditary mutation of coagulation factor XII (FXII) in a consanguineous Chinese family. METHODS: Mutations were investigated using Sanger and whole-exome sequencing. FXII (FXII:C) activity and FXII antigen (FXII:Ag) were assessed using clotting assays and ELISA, respectively. Gene variants were annotated and the likelihood that amino acid mutations would affect protein function was predicted using bioinformatics. RESULTS: Activated partial thromboplastin time was prolonged to >170 s (reference range, 22...
June 28, 2023: Clinical Biochemistry
Zhenguo Wu, Zaibao Liu, Qing Zhang, Hengzhe Zhang, Huiliang Cui, Yerui Zhang, Li Liu, Hongchun Wang, Jianmin Yang
BACKGROUND: Junctional adhesion molecule C (JAM-C) is a novel cell adhesion molecule that belongs to the immunoglobulin superfamily. Previous studies have demonstrated the up-regulation of JAM-C in atherosclerotic vessels in human and in spontaneous early lesions of apoe-/- mice. However, insufficient research is currently available on the association of plasma JAM-C levels with the presence and severity of coronary artery disease (CAD). OBJECTIVES: To explore the relationship between plasma JAM-C levels and CAD...
June 28, 2023: Clinical Biochemistry
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