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Clinical Biochemistry

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https://read.qxmd.com/read/30776353/the-prognostic-value-of-nitrotyrosine-levels-in-coronary-heart-disease-long-term-evaluation-in-the-acute-coronary-syndrome-registry-strategy-erico-study
#1
Alessandra V L Quidim, Tatiana Bruno, Paola Caroline Lacerda Leocádio, Itamar S Santos, Jacqueline Isaura Alvarez-Leite, Penélope Lacrísio Dos Reis Menta, Paulo A Lotufo, Isabela M Benseñor, Alessandra C Goulart
INTRODUCTION: We aimed to analyze the association of nitrotyrosine (N-TYR) levels and long-term survival in an ongoing coronary heart disease (CHD) prospective cohort, the Acute Coronary Syndrome Registry Strategy (ERICO study). METHODS: N-TYR levels collected during acute and subacute phase from onset of acute coronary syndrome (ACS) symptoms (myocardial infarction and unstable angina) were evaluated in 342 patients. We calculated case-fatality rates (180-days, 1 year, 2 years and 4 years) and survival analyses up to 4 years using Kaplan-Meier curves and Cox regression with respective cumulative hazard ratios (95% confidence interval; 95%CI), according to N-TYR tertiles up to 4 years of follow-up...
February 15, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30772277/slc22a3-is-associated-with-lipoprotein-a-concentration-and-cardiovascular-disease-in-familial-hypercholesterolemia
#2
Martine Paquette, Sophie Bernard, Alexis Baass
BACKGROUND AND AIMS: Several clinical and genetic factors have been shown to modulate the cardiovascular risk in subjects affected by familial hypercholesterolemia (FH). Genome wide association studies (GWAS) in the general population have identified several single nucleotide polymorphisms (SNPs) significantly associated with the risk of cardiovascular disease (CVD). This include the rs2048327 variant in the SLC22A3 gene. However, the effect of this SNP in FH subjects is unknown. The objectives of this study are to investigate the association between rs2048327 and the prevalence of CVD as well as with the concentration of lipoprotein (a) (Lp (a)), in a cohort of genetically-confirmed heterozygous FH patients...
February 14, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30771289/correct-implementation-of-the-hoffmann-method
#3
LETTER
Daniel T Holmes
No abstract text is available yet for this article.
February 13, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30763567/principal-component-and-correlation-analysis-of-biochemical-and-endocrine-markers-in-a-healthy-pediatric-population-caliper
#4
Victoria Higgins, Shabnam Hooshmand, Khosrow Adeli
BACKGROUND: Reference intervals (i.e. normative ranges) established from a healthy reference population are essential to accurately interpret disease biomarkers. Biomarker concentration may partially depend on associations with other biomarkers due to various physiological and pathophysiological processes. In this study, a robust correlation analysis was performed to identify physiological biomarker associations in the healthy pediatric CALIPER cohort METHODS: Population reference values for 35 biochemical and 20 fertility/endocrine markers were analyzed for correlations in all subjects, male adolescents, female adolescents, and young children...
February 11, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30753843/demethylation-of-the-hypoxia-induction-factor-1-binding-site-of-gpx3-at-excess-blood-ammonia-in-propionic-acidemia
#5
C B Item, A Schanzer, T Metz, S Greber-Platzer, J Lischka
OBJECTIVES: Elevated levels of metabolites such as ammonia and propionylcarnitine in propionic acidemia (PA) lead to an increased reactive oxygen species (ROS) production which could activate and stabilize the epigenetic regulated hypoxia-inducible factor-1α (HIF-1α). In order to evaluate the DNA methylation status of the HIF-1α binding site in PA, we investigated the antioxidant gluthatione peroxidase 3 gene (GPX3) promoter region. DESIGN AND METHODS: Using leukocyte DNA extracted from bloodspots collected 2-4 days after birth from diet free newborns, the cytosine phosphodiester bond guanine (CpG) dinucleotides of a HIF-1α binding site (CGTTTTTTACG) in the promoter region of GPX3 was retrospectively analysed...
February 9, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30738031/a-false-positive-case-of-high-sensitivity-cardiac-troponin-in-a-patient-with-acute-chest-pain-analytical-study-of-the-interference
#6
S Baroni, E Troiani, C Santonocito, G Moretti, C De Luca, M Antenucci, A Urbani
We report a case of a heterophile antibodies interference in a new high-sensitivity troponin commercial immunoassay (cTNIH Siemens), observed in a patient with possible acute coronary syndrome (ACS). The analytical interference was investigated with standard laboratories procedures. The false positive result was found with different troponin methods and kits. We also investigated the protein sequence of cTnl and no sequence variants were detected. The discordance between clinical pictures and high concentration of cTnl, together with the collaboration between clinicians and laboratory staff avoided possible erroneous diagnosis and further invasive investigations to the patient...
February 6, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30731070/cardiac-troponin-t-concentrations-and-patient-specific-risk-of-myocardial-infarction-using-the-novel-palfx-parameter
#7
Damiano Ognissanti, Christian Bjurman, Martin J Holzmann, Elvar Theodorsson, Max Petzold, Marija Cvijovic, Ola Hammarsten
BACKGROUND: Myocardial infarction (MI) is more likely if the heart damage biomarker cardiac troponin T (cTnT) is elevated in a blood sample from a patient with chest pain. There is no conventional method to estimate the risk of MI at a specific cTnT concentration. The purpose of this study was to evaluate the performance of a novel method that converts cTnT concentrations to patient-specific risks of MI. METHODS: Admission cTnT measurements in 15,425 ED patients from three hospitals with a primary complaint of chest pain, with or without a clinical diagnosis of MI, were Box-Cox-transformed to normality density functions to calculate the percentage with MI among patients with a given cTnT concentration, the parametric predictive value among lookalikes (PALfx)...
February 4, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30731069/insufficient-harmonization-of-antibiotics-assays-polish-experience-with-an-external-quality-assessment-program-in-the-years-2011-2018
#8
Robert Kowalski, Agnieszka Ćwiklińska, Marcin Lizakowski, Barbara Kortas-Stempak, Gabriela Bednarczuk, Aleksandra Fijałkowska, Piotr Pikul, Krzysztof Lewandowski
INTRODUCTION: Treatment with vancomycin and gentamycin requires strict monitoring of its serum concentration for proper dosage optimization. This study aimed to assess the quality and the harmonization of antibiotics assays in Polish laboratories. MATERIALS AND METHODS: 413 results of vancomycin and 148 results of gentamycin assays obtained from Polish laboratories in 30 international external quality assessment (EQA) surveys carried out from March 2011 to May 2018 were analyzed...
February 4, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30711389/impact-of-an-electronic-decision-support-rule-on-esr-crp-co-ordering-rates-in-a-community-health-system-and-projected-impact-in-the-tertiary-care-setting-and-a-commercially-insured-population
#9
Justin E Juskewitch, Andrew P Norgan, Ryan D Johnson, Vipul A Trivedi, Curtis A Hanson, Darci R Block
INTRODUCTION: Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) are common laboratory assays used as markers of inflammation. ESR suffers from higher false positive and false negative rates than CRP. To that end, the American Board of Internal Medicine's (ABIM's) Choosing Wisely campaign has recommended against ESR testing for those with undiagnosed conditions in favor of CRP testing. This study describes the impact of a computerized provider order entry (CPOE) decision support rule against ESR/CRP co-ordering within a community health system that predates the ABIM's Choosing Wisely national guidance...
January 31, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30707886/errors-in-measuring-plasma-free-fatty-acid-concentrations-with-a-popular-enzymatic-colorimetric-kit
#10
Yilin Song, Lianzhen Zhou, Michael D Jensen
OBJECTIVES: Our goal was to test whether an enzymatic, colorimetric assay, the WAKO NEFA kit, provides information equivalent to liquid chromatography (LC) LC-based measures of free fatty acid (FFA). DESIGN & METHODS: We reanalyzed nadir FFA samples from 109 volunteers from a previous study where we demonstrated that maximal suppression of FFA concentrations predicts metabolic abnormalities in humans; the results from the WAKO NEFA kit, which has been widely used for over three decades, could not replicate our findings...
January 29, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30695682/fingerstick-test-quantifying-humoral-and-cellular-biomarkers-indicative-for-m-leprae-infection
#11
Paul L A M Corstjens, Anouk van Hooij, Elisa M Tjon Kon Fat, Korshed Alam, Loes B Vrolijk, Sipho Dlamini, Moises Batista da Silva, John S Spencer, Claudio G Salgado, Jan Hendrik Richardus, Colette L M van Hees, Annemieke Geluk
OBJECTIVES: New user-friendly diagnostic tests for detection of individuals infected by Mycobacterium leprae (M. leprae), the causative pathogen of leprosy, can help guide therapeutic and prophylactic treatment, thus positively contributing to clinical outcome and reduction of transmission. To facilitate point-of-care testing without the presence of phlebotomists, the use of fingerstick blood (FSB) rather than whole blood-derived serum is preferred. This study is a first proof-of-principle validating that previously described rapid serum tests detecting antibodies and cytokines can also be used with FSB...
January 26, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30685208/aside-from-acute-renal-failure-cases-are-urinary-markers-of-glomerular-and-tubular-function-useful-in-clinical-practice
#12
REVIEW
Jean-Philippe Bastard, Soraya Fellahi, Axel Regeniter, Jacqueline Capeau, Pierre Ronco, Emmanuelle Plaisier
The qualitative evaluation of proteinuria represents a crucial diagnostic step in clinical practice for the classification of renal diseases according to glomerular, tubulo-interstitial, mixed injury or related to monoclonal gammopathy. Combined with the quantitative evaluation, it also allows an assessment of the disease's severity and prognosis as well as the response to treatment. The development of the urine protein profile (UPP) combines specific urine protein assays on a urine spot analyzing glomerular protein markers such as albumin, transferrin and immunoglobulin G, and tubular markers such as alpha-1microglobulin and retinol binding protein, to generate a detailed quantitative and qualitative proteinuria assessment...
January 24, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30684468/proteome-wide-onco-proteogenomic-somatic-variant-identification-in-er-positive-breast-cancer
#13
Lampros Dimitrakopoulos, Ioannis Prassas, Anieta M Sieuwerts, Eleftherios P Diamandis, John W M Martens, George S Charames
BACKGROUND: Recent advances in mass spectrometric instrumentation and bioinformatics have critically contributed to the field of proteogenomics. Nonetheless, whether that integrative approach has reached the point of maturity to effectively reveal the flow of genetic variants from DNA to proteins still remains elusive. The objective of this study was to detect somatically acquired protein variants in breast cancer specimens for which full genome and transcriptome data was already available (BASIS cohort)...
January 23, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30677402/further-assessment-of-the-prevalence-of-biotin-supplementation-and-its-impact-on-risk
#14
Christina M Trambas, Kwong Chun Liu, Hung Luu, Warren Louey, Catherine Lynch, Tina Yen, Kenneth A Sikaris
No abstract text is available yet for this article.
January 21, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30659791/unexpectedly-higher-diazoxon-hydrolysis-by-serum-paraoxonase-1-in-coronary-heart-disease
#15
Bharti Mackness, Mike Mackness
OBJECTIVES: Low serum PON1 activities (paraoxon, phenyl-acetate or lactone substrates) are associated with coronary heart disease (CHD). We investigated the rate of diazoxon hydrolysis by PON1 in a population with CHD. DESIGN & METHODS: Case- control study of 410 subjects with CHD and 274 controls. PON1 activity towards paraoxon and diazoxon, PON1 serum concentration and the PON1-55 and 192 polymorphisms were determined. RESULTS: There were no differences in the distribution of the PON1-55 or PON1-192 genotypes between the CHD and controls, however, PON1 activity towards diazoxon (DIAZ) was significantly (+160%) higher in CHD...
January 16, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30653948/circulating-mir-26a-mir-106b-mir-107-and-mir-133b-stratify-hepatocellular-carcinoma-patients-according-to-their-response-to-transarterial-chemoembolization
#16
Hamdy E A Ali, Ahmed A Emam, Ahmed A Zeeneldin, Reham Srour, Reda Tabashy, Eman D El-Desouky, Zakaria Y Abd Elmageed, Abdel-Hady A Abdel-Wahab
BACKGROUND: A number of hepatocellular carcinoma (HCC) patients have developed resistance against transcatheter arterial chemoembolization (TACE) treatment. In this study, we aimed to develop a panel of microRNAs (miRs) biomarkers to predict clinical outcomes in HCC patients after TACE treatment. METHODS: The expression level of twenty miRs was evaluated in FFPE tissues collected from 33 HCC patients. We selected four differentially expressed miRs in TACE-responders versus non-responders and re-assessed their expression in 51 serum samples...
January 14, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30633878/impact-of-clinical-sample-handling-and-processing-on-ultra-low-level-measurements-of-plasma-cytokines
#17
Limor Cohen, Alissa Keegan, Stacy E F Melanson, David R Walt
OBJECTIVES: In this study, we evaluated the impact of clinical sample handling and processing on IL-6, IL-10, IFNγ, and IL-2 measurements in plasma. DESIGN AND METHODS: We collected whole blood samples and analyzed various pre-analytical parameters. We assessed the following: 1) cytokine stability in whole blood that was stored over a ten-hour period at room temperature and 4 °C; 2) cytokine stability in plasma over 6 h; 3) vigorous sample handling including repeated dropping and transport through a pneumatic transport system; and 4) freeze-thaw stability of cytokines in plasma...
January 8, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30629956/comparative-accuracy-of-optical-sensor-based-wearable-system-for-non-invasive-measurement-of-blood-glucose-concentration
#18
Dmitry Rodin, Michael Kirby, Natallia Sedogin, Yair Shapira, Albert Pinhasov, Anatoly Kreinin
Non-invasive biosensors for indirect evaluation of routinely-measured blood components by sweat analysis have broad potential clinical applications. This trial tested a wrist-borne non-invasive glucose monitor (NIGM) to measure blood glucose (BG) levels using photoplethysmographic (PPG) optical sensors. Our aim was to determine the accuracy of the device in comparison to the standard, invasive clinical methods for blood glucose monitoring. Adult participants (n = 210) of both sexes from 18 to 75 were recruited for the study...
January 7, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30615855/estimated-costs-of-51-commonly-ordered-laboratory-tests-in-canada
#19
Irene Ma, Cheryl K Lau, Zane Ramdas, Rhonda Jackson, Christopher Naugler
INTRODUCTION: Test cost display has been shown to reduce inappropriate laboratory test ordering practices in the United States. Unfortunately, such a system is limited in the Canadian publically funded healthcare environment. Many Canadian physicians inaccurately estimate the cost of laboratory tests, which may contribute to mis-utilization. Here, we provide an estimated cost of over 50 commonly ordered laboratory tests in Canada as an educational tool for physicians. METHODS: Test volume data was collected from Calgary Laboratory Services' Laboratory Information System in order to determine which laboratory and diagnostic tests are most commonly ordered in Calgary and its surrounding area...
January 5, 2019: Clinical Biochemistry
https://read.qxmd.com/read/30592989/high-sensitivity-c-reactive-protein-and-cystatin-c-independently-and-jointly-predict-all-cause-mortality-among-the-middle-aged-and-elderly-chinese-population
#20
Yueping Shen, Yuxia Zhang, Suting Xiong, Xiaohong Zhu, Chaofu Ke
Studies investigating the relationship between high-sensitivity C-reactive protein (hs-CRP), cystatin C, and all-cause mortality yielded inconsistent results. Moreover, the joint effect of hs-CRP and cystatin C on mortality risk is largely unknown for the general population. In this study, we examined the associations between hs-CRP, cystatin C, and all-cause mortality using data from the China Health and Retirement Longitudinal Study (CHARLS). Middle-aged and elderly participants with complete data were enrolled for a 4-year follow-up of total mortality and plasma levels of hs-CRP (n = 11,409) and cystatin C (n = 8680)...
December 25, 2018: Clinical Biochemistry
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