journal
https://read.qxmd.com/read/38381358/albiflorin-alleviates-severe-acute-pancreatitis-associated-liver-injury-by-inactivating-p38mapk-nf-%C3%AE%C2%BAb-signaling-pathway
#1
JOURNAL ARTICLE
Haitao Li, Xiangpeng Zeng, Dongjie Sun, Xingfeng Qi, Dazhou Li, Wen Wang, Yan Lin
Albiflorin (Alb) is a monoterpenoid component that is commonly found in Paeonia lactiflora Pall. or Paeonia veitchii Lynch. It is known for its impressive anti-oxidant and anti-inflammatory properties. However, the effect of Alb on severe acute pancreatitis (SAP)-associated liver injury has not been fully understood. To investigate this, we conducted a study using a rat model of SAP induced by administering two intraperitoneal injections of 20% L-arginine (3.3 g/kg) over a period of 2 h. Subsequently, the SAP-induced rats were randomly assigned into different groups with the treatment of gradient doses of Alb (5, 10, and 20 mg/kg), with the normal saline as the sham group...
February 21, 2024: Biochemical Genetics
https://read.qxmd.com/read/38381357/long-non-coding-rna-02298-promotes-the-malignancy-of-hcc-by-targeting-the-mir-28-5p-ccdc6-pathway
#2
JOURNAL ARTICLE
Jinyi Wang, Bin Xu, Litao Liang, Qi Chen
Hepatocellular carcinoma (HCC) is a malignancy characterized by a high fatality rate. Increasing evidence indicating that long non-coding RNAs (lncRNAs) play a regulatory role in hepatocellular carcinoma (HCC). Among them, the correlation between LINC02298 and HCC remains unknown. The expression and subcellular localization of LINC02298 in HCC tissues and cell lines were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Furthermore, the correlation between the expression of LINC02298 and clinicopathological features of HCC patients was analyzed...
February 21, 2024: Biochemical Genetics
https://read.qxmd.com/read/38379039/daturataturin-a-ameliorates-psoriasis-by-regulating-ppar-pathway
#3
JOURNAL ARTICLE
Zheng Wei, Hongfa Zhong, Shanmin Yuan, Cong Chen
Psoriasis is a kind of severe immune-mediated systemic skin disorder, becoming a worldwide public health concern. Daturataturin A (DTA), a withanolide compound, exerts excellent anti-inflammatory and anti-proliferative properties. The objective of this study is to elucidate the effect of DTA on psoriasis and its potential mechanism. We established psoriasis-like keratinocytes model by stimulating HaCaT cells with M5 cocktail cytokines including Interleukin (IL)-17A, IL-22, oncostatin M, IL-1α, and tumor necrosis factor-α (TNF-α), followed by intervention with DTA...
February 20, 2024: Biochemical Genetics
https://read.qxmd.com/read/38379038/propofol-ameliorates-spinal-cord-injury-process-by-mediating-mir-672-3p-tnip2-axis
#4
JOURNAL ARTICLE
Chengliang Sun, Dongzhi Liu, Shunheng Gao, Mingyu Xiu, Zhaojian Zhang
Propofol has been found to have a protective effect against spinal cord injury (SCI). However, the underlying molecular mechanism of propofol regulating SCI process remains unclear. In this study, lipopolysaccharide (LPS)-induced PC12 cells were used to build SCI cell models. Cell viability and apoptosis were determined by cell counting kit 8 assay, flow cytometry, and caspase-3 activity detection. The protein levels of apoptosis-related markers and TNFAIP3 interacting protein 2 (TNIP2) were assessed using western blot analysis, and the levels of inflammatory factors were detected using ELISA...
February 20, 2024: Biochemical Genetics
https://read.qxmd.com/read/38379037/silencing-kpna2-promotes-ferroptosis-in-laryngeal-cancer-by-activating-the-foxo-signaling-pathway
#5
JOURNAL ARTICLE
Mimi Xu, Xiaoqi Hu, Zhixue Xiao, Siyi Zhang, Zhongming Lu
We aim to clarify the specific role of Karyopherin α2 (KPNA2) in the progression of laryngeal cancer, a kind of malignant tumor with a poor curative effect. We performed the bioinformatic analysis to obtain the ferroptosis-related differentially expressed genes. KPNA2 was screened out. Then the CCK-8 assay, wound healing assay, and transwell assay were used to clarify the changes in the proliferation, migration, and invasion abilities of laryngeal cancer cells after silencing KPNA2. The concentrations of iron ions, glutathione, superoxide dismutase, and malondialdehyde were evaluated by the corresponding detection kits...
February 20, 2024: Biochemical Genetics
https://read.qxmd.com/read/38379036/the-role-of-mir-29a-and-mir-143-on-the-anti-apoptotic-mcl-1-ciap-2-genes-expression-in-egfr-mutated-non-small-cell-lung-carcinoma-patients
#6
JOURNAL ARTICLE
Zahra Abrehdari-Tafreshi, Ehsan Arefian, Nasser Rakhshani, S Mahmoud A Najafi
The survival rate of lung cancer is low due to the high frequency of drug resistance in patients with mutations in the driver genes. Overexpression of anti-apoptotic genes is one of the most prominent features of tumor drug resistance. EGFR signaling induces the expression of anti-apoptotic genes. Also, microRNAs (miRNAs) have a critical role in regulating biological functions such as apoptosis; a process mostly eluded in cancer progression. The mutation screening was performed on one thousand non-small cell lung carcinoma patients to enroll clinical samples in this study...
February 20, 2024: Biochemical Genetics
https://read.qxmd.com/read/38376578/circ-luc7l-absence-attenuates-diabetic-nephropathy-progression-by-reducing-mesangial-cell-excessive-proliferation-inflammation-and-extracellular-matrix-accumulation-via-mediating-the-mir-205-5p-tgfbr1-pathway
#7
JOURNAL ARTICLE
Zhan Fang, Dan Wang, Fang Sun, Jing Chang, Dong Yuan, Shuhua Lin, Jian Teng
Diabetic nephropathy (DN) threatens the survival quality of patients, with complex pathogenesis. Circular RNA (circRNA) dysregulation occurs in DN development. This work aimed to investigate the role of circ-Luc7l in DN cell models and related molecular mechanisms. The expression of circ-Luc7l, microRNA (miR)-205-5p, and transforming growth factor-beta receptor 1 (Tgfbr1) was examined by real-time quantitative PCR (RT-qPCR). Cell viability and proliferation were detected by Cell Counting Kit-8 (CCK-8) assay and EdU assay...
February 20, 2024: Biochemical Genetics
https://read.qxmd.com/read/38376577/circ-rnf111-promotes-proliferation-of-ovarian-cancer-cell-skov-3-by-targeting-the-mir-556-5p-ccnd1-axis
#8
JOURNAL ARTICLE
Li Zuo, Yue Tan, Qiao-Ling Xu, Xiao-Li Li, Mi Xiao
The aim of this study was to investigate the role and mechanism of circ-RNF111 in the human ovarian cancer cell line SKOV-3. First, qRT-PCR was used to detect circ-RNF111 and miR-556-5p expression levels in human normal ovarian epithelial cells IOSE80 and human ovarian cancer cells SKOV-3. CCK-8 and colony formation assays were adopted to determine the proliferation rate and cell viability of SKOV-3 cells, respectively. Additionally, in an attempt to reveal the mechanism of circ-RNF111, we predicted the targeting relationship between miR-556-5p and circ-RNF111 as well as miR-556-5p and CCND1 using the circinteractome and TargetScan databases, respectively, and validated their relationship by dual-luciferase reporter assay...
February 20, 2024: Biochemical Genetics
https://read.qxmd.com/read/38374521/genes-and-pathways-underpinning-klinefelter-syndrome-at-bulk-and-single-cell-levels
#9
JOURNAL ARTICLE
Linlin Tian, Yan Yu, Ziqing Mao, Dandan Xu, Hongbo Zhang, Mengkai Qiao, Tong Chen, Wen Liu
Klinefelter syndrome (KS) is the most frequent genetic anomaly in infertile men. Given its unclear mechanism, we aim to investigate critical genes and pathways in the pathogenesis of KS based on three bulk and one single-cell transcriptome data sets from Gene Expression Omnibus. We merged two data sets (GSE42331 and GSE47584) with human KS whole blood samples. When comparing the control and KS samples, five hub genes, including defensin alpha 4 (DEFA4), bactericidal permeability increasing protein (BPI), myeloperoxidase (MPO), intelectin 1 (ITLN1), and Xg Glycoprotein (XG), were identified...
February 19, 2024: Biochemical Genetics
https://read.qxmd.com/read/38368567/effects-of-klf11-on-vascular-smooth-muscle-cells-and-its-underlying-mechanisms-in-intracranial-aneurysm
#10
JOURNAL ARTICLE
Ke Yan, Jiarong Bian, Liang He, Bingwei Song, Linhai Shen, Yong Zhen
Vascular smooth muscle cells (VSMCs) affect the phenotypic changes in intracranial aneurysm (IA). They exhibit enhanced dissociation and migration and play a key role in IA pathogenesis. KLF transcription factor 11 (KLF11), a member of the KLF family, significantly affects the cancer cell proliferation, differentiation, and apoptosis. However, its expression, biological functions, and latent action mechanisms in IA remain unclear. This study aimed to analyze the effects of KLF11 on H2 O2 -induced human brain VSMCs (HBVSMCs) in IA...
February 18, 2024: Biochemical Genetics
https://read.qxmd.com/read/38367128/dynamic-expressions-of-yellow-stripe-like-ysl-genes-during-pod-development-shed-light-on-associations-with-iron-distribution-in-phaseolus-vulgaris
#11
JOURNAL ARTICLE
Yihan Zhang, Yunxiu Liu, Hailan Li, Ting Sun, Min Xu, Pei Xu
The global prevalence of iron deficiency-induced "hidden hunger" highlights a critical health concern, underscoring the pressing need to improve iron nutrition through safe and efficient means, such as increasing iron intake from plant-based foods. Yellow Stripe-Like (YSL) genes play a crucial role in long-distance iron transport between source and sink tissues in plants. Here, we report on the analysis of YSL family genes in the common bean (Phaseolus vulgaris L.), an iron-rich legume crop. We identified 9 YSL genes in the common bean genome using BLAST and HMM methods...
February 17, 2024: Biochemical Genetics
https://read.qxmd.com/read/38363412/occurrence-of-existing-bcr-abl-baseline-mutations-and-associated-haplotype-nmr-among-cml-patients-with-diverse-im-response-a-hospital-based-study-from-north-east-india
#12
JOURNAL ARTICLE
Gautam Hazarika, Manash Jyoti Kalita, Partha Pratim Das, Simanta Kalita, Kalpajit Dutta, Lipika Lahkar, Anjanjyoti Rajkonwar, Mohammed Ghaznavi Idris, Vinotsale Khamo, Giriraj Kusre, Subhash Medhi
Highly polymorphic BCR-ABL kinase domains have been reported to harbor more than a hundred mutations, and among these, 40-60% have been identified as influencers of imatinib mesylate (IM) resistance. The emergence of IM resistance poses a significant challenge in the management of Chronic Myeloid Leukemia (CML). M351T (rs121913457), E255K (rs387906517), and Y253H (rs121913461) are of particular clinical significance due to their association with high-level imatinib resistance. This study was conducted to investigate the potential role of three significant SNPs in CML progression due to IM resistance...
February 16, 2024: Biochemical Genetics
https://read.qxmd.com/read/38361095/uncovering-the-cerna-network-related-to-the-prognosis-of-stomach-adenocarcinoma-among-898-patient-samples
#13
JOURNAL ARTICLE
Zhe Liu, Fang Liu, Olutomilayo Olayemi Petinrin, Fuzhou Wang, Yu Zhang, Ka-Chun Wong
Stomach adenocarcinoma (STAD) patients are often associated with significantly high mortality rates and poor prognoses worldwide. Among STAD patients, competing endogenous RNAs (ceRNAs) play key roles in regulating one another at the post-transcriptional stage by competing for shared miRNAs. In this study, we aimed to elucidate the roles of lncRNAs in the ceRNA network of STAD, uncovering the molecular biomarkers for target therapy and prognosis. Specifically, a multitude of differentially expressed lncRNAs, miRNAs, and mRNAs (i...
February 15, 2024: Biochemical Genetics
https://read.qxmd.com/read/38358588/exploring-soysnp50k-and-usda-germplasm-collection-data-to-find-new-qtls-associated-with-protein-and-oil-content-in-brazilian-genotypes
#14
JOURNAL ARTICLE
Jessica Nayara Basílio Silva, Rafael Delmond Bueno, Teresinha de Jesus Feitosa de Sousa, Yan Pablo Moreira Xavier, Luiz Claudio Costa Silva, Newton Deniz Piovesan, Cleberson Ribeiro, Maximiller Dal-Bianco
Genetic diversity within a germplasm collection plays a vital role in the success of breeding programs. However, comprehending this diversity and identifying accessions with desirable traits pose significant challenges. This study utilized publicly available data to investigate SNP markers associated with protein and oil content in Brazilian soybeans. Through this research, twenty-two new QTLs (Quantitative Trait Loci) were identified, and we highlighted the substantial influence of Roanoke, Lee and Bragg ancestor on the genetic makeup of Brazilian soybean varieties...
February 15, 2024: Biochemical Genetics
https://read.qxmd.com/read/38358587/circrna-itch-inhibits-epithelial-mesenchymal-transformation-and-promotes-apoptosis-in-papillary-thyroid-carcinoma-via-mir-106a-5p-jazf1-axis
#15
JOURNAL ARTICLE
Yijun Chen, Zhiming Lian, Guolie Zhang, Yuanmei Lin, Guoliang Zhang, Wei Liu, Jian Gao, Zifang Zheng
Circular RNA ITCH (circ-ITCH) is implicated in papillary thyroid carcinoma (PTC) development. Nevertheless, the more detailed molecular mechanism remains uncovered. The transcriptional level of circ-ITCH was tested via quantitative real-time PCR. Transwell assay was introduced to assess the migrative and invasive abilities of cells. RNA interference technology was employed to reduce the level of circ-ITCH as well as JAZF1 in PTC cells. Western blot assay was utilized to reveal the content of JAZF1 and proteins related to epithelial-mesenchymal transformation (EMT) progression...
February 15, 2024: Biochemical Genetics
https://read.qxmd.com/read/38356009/global-dna-methylation-levels-viz-a-viz-genetic-and-biochemical-variations-in-one-carbon-metabolic-pathway-an-exploratory-study-from-north-india
#16
JOURNAL ARTICLE
Vineet Chaudhary, Debashis Bhattacharjee, Naorem Kiranmala Devi, Kallur Nava Saraswathy
Despite the importance of one carbon metabolic pathway (OCMP) in modulating the DNA methylation process, only a few population-based studies have explored their relationship among healthy individuals. This study aimed to understand the variations in global DNA methylation levels with respect to selected genetic (CBS 844ins68, MTRR A66G, MTR A2756G, and MTHFR C677T polymorphisms) and biochemical (folate, vitamin B12, and homocysteine) markers associated with OCMP among healthy North Indian adults. The study has been conducted among 1095 individuals of either sex (69...
February 14, 2024: Biochemical Genetics
https://read.qxmd.com/read/38353892/a-machine-learning-computational-framework-develops-a-multiple-programmed-cell-death-index-for-improving-clinical-outcomes-in-bladder-cancer
#17
JOURNAL ARTICLE
Chunhong Li, Wangshang Qin, Jiahua Hu, Jinxia Lin, Yiming Mao
Comprehensive action patterns of programmed cell death (PCD) in bladder cancer (BLCA) have not yet been thoroughly investigated. Here, we collected 19 different PCD patterns, including 1911 PCD-related genes, and developed a multiple programmed cell death index (MPCDI) based on a machine learning computational framework. We found that in the TCGA-BLCA training cohort and the independently validated GSE13507 cohort, the patients with high-MPCDI had a worse prognosis, whereas patients with low-MPCDI had a better prognosis...
February 14, 2024: Biochemical Genetics
https://read.qxmd.com/read/38349440/identification-of-central-genes-and-regulatory-pathways-associated-with-hyperlipidemia-in-rats
#18
JOURNAL ARTICLE
Xinyue Fu, Xinni Song, Shufang Niu, Jiali Liu, Songli Shi, Hong Chang, Chuanming Zhang, Jun Qi, Peng Wang, Yingchun Bai, Wanfu Bai
Hyperlipidemia is an independent risk factor for cardiovascular and cerebrovascular diseases. The transcriptomic data and the gene regulatory networks of hyperlipidemia are largely unclear. We analyzed the changes in liver gene expression and the serum levels of biochemical indicators in rats with hyperlipidemia induced by high-fat diet (HFD). The body weight, liver weight, and the serum levels of TG, TC, HDL-C, LDL-C, ALT, and AST were significantly higher in the hyperlipidemic rats compared to the healthy controls (P < 0...
February 13, 2024: Biochemical Genetics
https://read.qxmd.com/read/38349439/comprehensive-analysis-of-the-sumo-related-signature-implication-for-diagnosis-prognosis-and-immune-therapeutic-approaches-in-cervical-cancer
#19
JOURNAL ARTICLE
Xing Zhang, Jian Cao, Xiuting Li, Yan Zhang, Wenjing Yan, Bo Ding, Jing Hu, Haohan Liu, Xue Chen, Yamei Nie, Fengying Liu, Ning Lin, Shizhi Wang
SUMOylation, an important post-translational protein modification, plays a critical role in cancer development and immune processes. This study aimed to construct diagnostic and prognostic models for cervical cancer (CC) using SUMOylation-related genes (SRGs) and explore their implications for novel clinical therapies. We analyzed the expression profiles of SRGs in CC patients and identified 15 SRGs associated with CC occurrence. After the subsequent qPCR verification of 20 cases of cancer and adjacent tissues, 13 of the 15 SRGs were differentially expressed in cancer tissues...
February 13, 2024: Biochemical Genetics
https://read.qxmd.com/read/38349438/the-association-between-genetic-variants-in-ace1and-ace2-genes-with-susceptibility-to-covid-19-infection
#20
JOURNAL ARTICLE
Hamid Abbaszadeh, Hamid Kabiri-Rad, Fariba Mohammadi, Soheila Zangoie, Mahdieh Rajabi-Moghaddam, Shokouh Ghafari, Masood Ziaee, Davod Javanmard, Ebrahim Miri-Moghaddam
Angiotensin-converting enzyme 2 (ACE2) receptors facilitate the entry of the causative virus severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) into target cells. Some ACE gene variants have been suggested to be involved in COVID-19 pathogenesis. So, the aim was to assess the association between ACE1 rs4646994 and ACE2 rs2285666 genes polymorphisms and the susceptibility and severity of COVID-19. This case-control study was conducted on 197 patients with COVID-19 and 197 healthy controls...
February 13, 2024: Biochemical Genetics
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