journal
https://read.qxmd.com/read/36946735/characterization-of-the-impact-of-merkel-cell-polyomavirus-induced-interferon-signaling-on-viral-infection
#1
JOURNAL ARTICLE
Ranran Wang, June F Yang, Taylor E Senay, Wei Liu, Jianxin You
Merkel cell polyomavirus (MCPyV) has been associated with approximately 80% of Merkel cell carcinoma (MCC), an aggressive and increasingly incident skin cancer. The link between host innate immunity, viral load control, and carcinogenesis has been established but poorly characterized. We previously established the importance of the STING and NF-κB pathways in the host innate immune response to viral infection. In this study, we further discovered that MCPyV infection of human dermal fibroblasts (HDFs) induces the expression of type I and III interferons (IFNs), which in turn stimulate robust expression of IFN-stimulated genes (ISGs)...
March 22, 2023: Journal of Virology
https://read.qxmd.com/read/36943134/archaeal-kink-turn-binding-protein-mediates-inhibition-of-orthomyxovirus-splicing-biology
#2
JOURNAL ARTICLE
Kohei Oishi, Daniel Blanco-Melo, Andrew P Kurland, Jeffrey R Johnson, Benjamin R tenOever
Despite lacking a DNA intermediate, orthomyxoviruses complete their replication cycle in the nucleus and generate multiple transcripts by usurping the host splicing machinery. This biology results in dynamic changes of relative viral transcripts over time and dictates the replicative phase of the infection. Here, we demonstrate that the family of archaeal L7Ae proteins uniquely inhibit the splicing biology of influenza A virus, influenza B virus, and Salmon isavirus, revealing a common strategy utilized by Orthomyxoviridae members to achieve this dynamic...
March 21, 2023: Journal of Virology
https://read.qxmd.com/read/36943070/structural-study-of-the-cobetia-marina-bacteriophage-1-carin-1-by-cryo-em
#3
JOURNAL ARTICLE
Alessio d'Acapito, Thomas Roret, Eleftherios Zarkadas, Pierre-Yves Mocaër, Florian Lelchat, Anne-Claire Baudoux, Guy Schoehn, Emmanuelle Neumann
Most of studied bacteriophages (phages) are terrestrial viruses. However, marine phages are shown to be highly involved in all levels of oceanic regulation. They are, however, still largely overlooked by the scientific community. By inducing cell lysis on half of the bacterial population daily, their role and influence on the bacterial biomass and evolution, as well as their impact in the global biogeochemical cycles, is undeniable. Cobetia marina virus 1 (Carin-1) is a member of the Podoviridae family infecting the γ -protoabacteria C ...
March 21, 2023: Journal of Virology
https://read.qxmd.com/read/36943056/structure-of-the-spring-viraemia-of-carp-virus-ribonucleoprotein-complex-reveals-its-assembly-mechanism-and-application-in-antiviral-drug-screening
#4
JOURNAL ARTICLE
Zhao-Xi Wang, Bing Liu, Tian Yang, Daqi Yu, Chu Zhang, Liming Zheng, Jin Xie, Bin Liu, Mengxi Liu, Hailin Peng, Luhua Lai, Qi Ouyang, Songying Ouyang, Yong-An Zhang
Spring viremia of carp virus (SVCV) is a highly pathogenic Vesiculovirus infecting the common carp, yet neither a vaccine nor effective therapies are available to treat spring viremia of carp (SVC). Like all negative-sense viruses, SVCV contains an RNA genome that is encapsidated by the nucleoprotein (N) in the form of a ribonucleoprotein (RNP) complex, which serves as the template for viral replication and transcription. Here, the three-dimensional (3D) structure of SVCV RNP was resolved through cryo-electron microscopy (cryo-EM) at a resolution of 3...
March 21, 2023: Journal of Virology
https://read.qxmd.com/read/36943051/psmb4-degrades-the-porcine-reproductive-and-respiratory-syndrome-virus-nsp1%C3%AE-protein-via-the-autolysosome-pathway-and-induces-the-production-of-type-i-interferon
#5
JOURNAL ARTICLE
Heyou Yi, Qiumei Wang, Lechen Lu, Ruirui Ye, Ermin Xie, Zhiqing Yu, Yankuo Sun, Yao Chen, Mengkai Cai, Yingwu Qiu, Qianwen Wu, Jie Peng, Heng Wang, Guihong Zhang
Porcine reproductive and respiratory syndrome virus (PRRSV) causes respiratory disease in pigs of all ages and reproductive failure in sows, resulting in great economic losses to the swine industry. In this work, we identified the interaction between PSMB4 and PRRSV Nsp1α by yeast two-hybrid screening. The PSMB4-Nsp1α interaction was further confirmed by coimmunoprecipitation, glutathione S -transferase (GST) pulldown, and laser confocal experiments. The PCPα domain (amino acids 66 to 166) of Nsp1α and the C-terminal domain (amino acids 250 to 264) of PSMB4 were shown to be critical for the PSMB4-Nsp1α interaction...
March 21, 2023: Journal of Virology
https://read.qxmd.com/read/36939350/harnessing-the-noncanonical-keap1-nrf2-pathway-for-human-cytomegalovirus-control
#6
JOURNAL ARTICLE
Ayan K Ghosh, Yu-Pin Su, Michael Forman, Robert F Keyes, Brian C Smith, Xin Hu, Marc Ferrer, Ravit Arav-Boger
Host-derived cellular pathways can provide an unfavorable environment for virus replication. These pathways have been a subject of interest for herpesviruses, including the betaherpesvirus human cytomegalovirus (HCMV). Here, we demonstrate that a compound, ARP101, induces the noncanonical sequestosome 1 (SQSTM1)/p62-Keap1-Nrf2 pathway for HCMV suppression. ARP101 increased the levels of both LC3 II and SQSTM1/p62 and induced phosphorylation of p62 at the C-terminal domain, resulting in its increased affinity for Keap1...
March 20, 2023: Journal of Virology
https://read.qxmd.com/read/36939341/rift-valley-fever-virus-nucleoprotein-triggers-autophagy-to-dampen-antiviral-innate-immune-responses
#7
JOURNAL ARTICLE
Xiangtao Zhu, Zihan Guan, Yujie Fang, Yulan Zhang, Zhenqiong Guan, Shufen Li, Ke Peng
Rift Valley fever virus (RVFV) is a mosquito-borne bunyavirus that causes severe and potentially fatal hemorrhagic fever in humans. Autophagy is a self-degradative process that can restrict viral replication at multiple infection steps. In this study, we evaluated the effects of RVFV-triggered autophagy on viral replication and immune responses. Our results showed that RVFV infection triggered autophagosome formation and induced complete autophagy. Impairing autophagy flux by depleting autophagy-related gene 5 ( ATG5 ), ATG7 , or sequestosome 1 ( SQSTM1 ) or treatment with autophagy inhibitors markedly reduced viral RNA synthesis and progeny virus production...
March 20, 2023: Journal of Virology
https://read.qxmd.com/read/36916989/cathelicidins-target-hsp60-to-restrict-cvb3-transmission-via-disrupting-the-exosome-and-reducing-cardiomyocyte-apoptosis
#8
JOURNAL ARTICLE
Yang Yang, Chunjing Huang, Li Hui, Yahui Song, Yuxuan Fu, Min Li, Hailong Yang, Jing Wu, Jia Sun, Wei Xu, Lin Wei
Cathelicidin antimicrobial peptides (mouse, CRAMP; human, LL-37) have broad-spectrum antiviral activities against enveloped viruses, but their mechanisms of action against nonenveloped viruses remain to be elucidated. Coxsackievirus B3 (CVB3), a member of nonenveloped virus belonging to the Enterovirus genus of Picornaviridae , is an important pathogen of viral myocarditis and dilated cardiomyopathy. Here, we observed that cardiac CRAMP expression was significantly upregulated in mice after CVB3 infection. The administration of CRAMP or LL-37 markedly suppressed CVB3 infection in mice, and CRAMP deficiency increased the susceptibility of mice to CVB3...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916948/exploring-host-binding-machineries-of-mycobacteriophages-with-alphafold2
#9
JOURNAL ARTICLE
Christian Cambillau, Adeline Goulet
Although more than 12,000 bacteriophages infecting mycobacteria (mycobacteriophages) have been isolated so far, there is a knowledge gap on their structure-function relationships. Here, we have explored the architecture of host-binding machineries from seven representative mycobacteriophages of the Siphoviridae family infecting Mycobacterium smegmatis, Mycobacterium abscessus, and Mycobacterium tuberculosis, using AlphaFold2 (AF2). AF2 enables confident structural analyses of large and flexible biological assemblies resistant to experimental methods, thereby opening new avenues to shed light on phage structure and function...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916947/the-failure-of-aids-vaccine-efficacy-trials-where-to-go-from-here
#10
JOURNAL ARTICLE
Ronald C Desrosiers
The seven AIDS vaccine efficacy trials have yielded extremely disappointing results at great expense. Greater stringency is needed for government support of AIDS vaccine efficacy trials.
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916940/molluscum-contagiosum-virus-protein-mc008-targets-nf-%C3%AE%C2%BAb-activation-by-inhibiting-ubiquitination-of-nemo
#11
JOURNAL ARTICLE
Thomas Phelan, Clara Lawler, Andreas Pichlmair, Mark A Little, Andrew G Bowie, Gareth Brady
Molluscum contagiosum virus (MCV) is a human-adapted poxvirus that causes a common and persistent yet mild infection characterized by distinct, contagious, papular skin lesions. These lesions are notable for having little or no inflammation associated with them and can persist for long periods without an effective clearance response from the host. Like all poxviruses, MCV encodes potent immunosuppressive proteins that perturb innate immune pathways involved in virus sensing, the interferon response, and inflammation, which collectively orchestrate antiviral immunity and clearance, with several of these pathways converging at common signaling nodes...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916938/uhrf1-deficiency-inhibits-alphaherpesvirus-through-inducing-rig-i-irf3-mediated-interferon-production
#12
JOURNAL ARTICLE
Mengdong Wang, Jingjing Song, Chao Gao, Cuilian Yu, Chao Qin, Yue Lang, Aotian Xu, Yun Liu, Wenhai Feng, Jun Tang, Rui Zhang
Type I interferon (IFN-I) response plays a prominent role in innate immunity, which is frequently modulated during viral infection. Here, we report DNA methylation regulator UHRF1 as a potent negative regulator of IFN-I induction during alphaherpesvirus infection, whereas the viruses in turn regulates the transcriptional expression of UHRF1. Knockdown of UHRF1 in cells significantly increases interferon-β (IFN-β)-mediated gene transcription and viral inhibition against herpes simplex virus 1 (HSV1) and pseudorabies virus (PRV)...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916936/ythdf2-is-downregulated-in-response-to-host-shutoff-induced-by-dna-virus-infection-and-regulates-interferon-stimulated-gene-expression
#13
JOURNAL ARTICLE
Charles R Hesser, Derek Walsh
Recent studies have begun to reveal the complex and multifunctional roles of N 6 -methyladenosine (m6 A) modifications and their associated writer, reader, and eraser proteins in infection by diverse RNA and DNA viruses. However, little is known about their regulation and functions during infection by several viruses, including poxviruses. Here, we show that members of the YTH Domain Family (YTHDF), in particular YTHDF2, are downregulated as the prototypical poxvirus, vaccinia virus (VacV) enters later stages of replication in a variety of natural target cell types, but not in commonly used transformed cell lines wherein the control of YTHDF2 expression appears to be dysregulated...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916924/strain-dependent-restriction-of-human-cytomegalovirus-by-zinc-finger-antiviral-proteins
#14
JOURNAL ARTICLE
Maria Jose Lista, Adam A Witney, Jenna Nichols, Andrew J Davison, Harry Wilson, Katie A Latham, Benjamin J Ravenhill, Katie Nightingale, Richard J Stanton, Michael P Weekes, Stuart J D Neil, Chad M Swanson, Blair L Strang
Cellular antiviral factors that recognize viral nucleic acid can inhibit virus replication. These include the zinc finger antiviral protein (ZAP), which recognizes high CpG dinucleotide content in viral RNA. Here, we investigated the ability of ZAP to inhibit the replication of human cytomegalovirus (HCMV). Depletion of ZAP or its cofactor KHNYN increased the titer of the high-passage HCMV strain AD169 but had little effect on the titer of the low-passage strain Merlin. We found no obvious difference in expression of several viral proteins between AD169 and Merlin in ZAP knockdown cells, but observed a larger increase in infectious virus in AD169 compared to Merlin in the absence of ZAP, suggesting that ZAP inhibited events late in AD169 replication...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916919/regulation-of-virus-replication-by-bk-polyomavirus-small-t-antigen
#15
JOURNAL ARTICLE
Wei Zou, Michael J Imperiale
Polyomavirus small T antigen (tAg) plays important roles in regulating viral replication, the innate immune response, apoptosis, and transformation for SV40, Merkel cell polyomavirus (MCPyV), murine polyomavirus (MuPyV), and JC polyomavirus (JCPyV). However, the function of BK polyomavirus (BKPyV) tAg has been much less studied. Here, we constructed mutant viruses that do not express tAg, and we showed that, in contrast with other polyomaviruses, BKPyV tAg inhibits large T antigen (TAg) gene expression and viral DNA replication...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916914/bombyx-mori-nucleopolyhedrovirus-hijacks-multivesicular-body-as-an-alternative-envelopment-platform-for-budded-virus-egress
#16
JOURNAL ARTICLE
Congyue Yao, Shijia Pan, Ying Xu, Mengze Lu, Yating Zhao, Jiayao Huo, Bifang Hao, Jinshan Huang
Baculovirus budded virus (BV) acquires its envelope and viral membrane fusion proteins from the plasma membrane (PM) of the host cell during the budding process. However, this classical BV egress pathway has been questioned because an intracellularly localized membrane fusion protein, SPΔn GP64 (glycoprotein 64 [GP64] lacking the signal peptide [SP] n region), was assembled into the envelope to generate infective BVs in our recent studies. Here, we identify an additional pathway for Bombyx mori nucleopolyhedrovirus (BmNPV) BV assembly and release that differs, in part, from the currently accepted model for the egress pathway of baculovirus...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916912/a-single-surface-exposed-amino-acid-determines-differential-neutralization-of-aav1-and-aav6-by-human-alpha-defensins
#17
JOURNAL ARTICLE
Jessica M Porter, Mackenzi S Oswald, Anjali Sharma, Shanan Emmanuel, Austin Kansol, Antonette Bennett, Robert McKenna, Jason G Smith
Adeno-associated viruses (AAVs) are being developed as gene therapy vectors due to their low pathogenicity and tissue tropism properties. However, the efficacy of these vectors is impeded by interactions with the host immune system. One potential immune barrier to vector transduction is innate immune host defense peptides, such as alpha-defensins, which are potent antiviral agents against other nonenveloped viruses. To investigate the interaction between AAVs and alpha-defensins, we utilized two closely related AAV serotypes, AAV1 and AAV6...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916908/erratum-for-zhao-et-al-n-acetyltransferase-8-promotes-viral-replication-by-increasing-the-stability-of-enterovirus-71-nonstructural-proteins
#18
JOURNAL ARTICLE
Xiaohui Zhao, Huijun Yuan, Hang Yang, Yan Liu, Meng Xun, Xiaozhen Li, Tingting Fan, Bo Wu, Shangrui Guo, Hongliang Wang
No abstract text is available yet for this article.
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36916907/the-genetic-variation-of-porcine-reproductive-and-respiratory-syndrome-virus-replicase-protein-nsp2-modulates-viral-virulence-and-persistence
#19
JOURNAL ARTICLE
Can Kong, Dan Li, Yanxin Hu, Peng Gao, Yongning Zhang, Lei Zhou, Xinna Ge, Xin Guo, Jun Han, Hanchun Yang
Fast evolution in the field of the replicase nsp2 represents a most prominent feature of porcine reproductive and respiratory syndrome virus (PRRSV). Here, we determined its biological significance in viral pathogenesis by constructing interlineage chimeric mutants between the Chinese highly pathogenic PRRSV (HP-PRRSV) strain JXwn06 (lineage 8) and the low-virulent NADC30-like strain CHsx1401 (lineage 1). Replacement with nsp2 from JXwn06 was surprisingly lethal to the backbone virus CHsx1401, but combined substitution with the structural protein-coding region (SP) gave rise to viable virus CHsx1401-SPnsp2JX ...
March 14, 2023: Journal of Virology
https://read.qxmd.com/read/36897089/a-critical-analysis-of-the-evidence-for-the-sars-cov-2-origin-hypotheses
#20
EDITORIAL
James C Alwine, Arturo Casadevall, Lynn W Enquist, Felicia D Goodrum, Michael J Imperiale
When humans experience a new, devastating viral infection such as SARS-CoV-2, significant challenges arise. How should individuals as well as societies respond to the situation? One of the primary questions concerns the origin of the SARS-CoV-2 virus that infected and was transmitted efficiently among humans resulting in a pandemic. At first glance, the question appears straightforward to answer. However, the origin of SARS-CoV-2 has been the topic of substantial debate primarily because we do not have access to some relevant data...
March 10, 2023: Journal of Virology
journal
journal
21700
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.