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Reema Vareen Diengdoh, Suman Kumaria, Meera Chettri Das
Orchids and their sustainability are very important issues that need global conservation efforts. Paphiopedilum insigne (an endangered orchid), is one of the most excessively exploited species of orchids and is mentioned in the IUCN Red List and Appendix I of CITES. The prospect for conservation and commercialization of this species would be strengthened with the development of improved cryopreservation techniques. This study reports on successful cryopreservation of protocorms of P.insigne after cryopreservation using vitrification (Vit) and encapsulation-vitrification (E-Vit) techniques...
February 15, 2019: Cryobiology
Saeed Shokri, Seyyed Meisam Ebrahimi, Sanaz Ziaeipour, Reza Nejatbakhsh
Cryopreservation of sperms is common therapy but with multiple damages to sperms. The aim of this study was to assess the effect of insulin as a prosurvival factor on the most important functional parameters of human spermatozoa during cryopreservation. Semen samples were obtained from 15 normozoospermic men at age 25-40 years of old through masturbation. Cryopreservation of sperms was conducted along with adding 10, 100, 500 and 1000 (ng/ml) insulin and a control group was also considered by adding distilled water...
February 11, 2019: Cryobiology
Stela Mari M Gheller, Carine D Corcini, Camila R C de Brito, Izani B Acosta, Geórgia C Tavares, Sara Lorandi Soares, Alessandra C Silva, Diego M Pires, Antonio Sergio Varela Junior
The current study assessed a semen cryopreservation protocol in the Amazonian catfish Leiarius marmoratus, a freshwater fish, of rheophilic behavior, and of great importance for Brazilian fish farming. Eight males (n = 8) were stripped and the semen was cryopreserved if total motility in fresh semen was higher than 80%. The external cryoprotectant Trehalose was then diluted in Beltsvile Thawing Solution (BTS) extender in the following concentrations: 50, 100, 150, and 200 mM. Semen samples were diluted in the media (1:9 v/v) being tested, then frozen in a container with nitrogen vapor (dryshipper), and stored in liquid nitrogen at -196 °C...
February 4, 2019: Cryobiology
Roman Franěk, Tomáš Tichopád, Christoph Steinbach, Xuan Xie, Jelena Lujić, Zoran Marinović, Ákos Horváth, Vojtěch Kašpar, Martin Pšenička
Several experiments were conducted in order to develop an optimal protocol for slow-rate freezing (-1 °C/min) and short-term storage (-80 or 4 °C) of common carp ovarian tissue fragments with an emphasis on oogonial stem cells (OSCs). Dimethyl sulfoxide (Me2 SO) with concentration of 1.5 M was identified as the best cryoprotectant in comparison to propylene glycol and methanol. When comparing supplementation of sugars (glucose, trehalose, sucrose) in different concentrations (0.1, 0.3, 0.5 M), glucose and trehalose in 0...
February 1, 2019: Cryobiology
Jong Kui Song, Yu Lan Hui
The purpose of the present study was to clarify the possibility of a rapid cryopreservation for cell line Paesun by cooling in the range of 30-40 °C/min to vapor phase of -120 ∼-140 °C before immersion into liquid phase of liquid nitrogen using 10% Me2 SO. After thawing, these cells were examined with assaying viability by trypan blue exclusion staining and survival by cloning in monolayer; the percentages of cell and colony recovery obtained in rapid cooling had a tendency to be lower than that by slow cooling of 1 °C/min but there were no significant differences between them...
January 31, 2019: Cryobiology
Chenzhuang Zhu, Sihao Lin, Junhao Liang, Yingjian Zhu
No abstract text is available yet for this article.
January 31, 2019: Cryobiology
Llanos Medrano, María Enciso, María José Gomez-Torres, Jon Aizpurua
PURPOSE: The purpose of this study is to present the first birth of healthy infant born following ICSI using the new permeable cryoprotectant-free sperm vitrification protocol Easy-Sperm® . PRINCIPAL RESULTS: A 39 years old woman and his 40 years old partner underwent egg donation treatment at IVF-Spain Alicante (Spain). Half of the mature oocytes obtained from a young and healthy donor were fertilized by ICSI, using slow-frozen spermatozoa and the other half with vitrified spermatozoa...
January 29, 2019: Cryobiology
F Bieuzen, C Hausswirth, B Dugué
As soluble intercellular adhesion molecule-1 (sICAM-1) was recently hypothesized to be a key player in the mechanisms involved in exercise-induced muscular damage (EIMD), we investigated its circulating concentration changes in athletes before and after EIMD with and without the use of whole-body cryostimulation (WBC; 3 min at -110 °C) at the exercise end and repeated once a day during 4 days. We previously characterized plasma specimens from 11 endurance athletes who performed twice (randomized crossover design) strenuous running leading to EIMD, followed by passive recovery or WBC...
January 29, 2019: Cryobiology
Zubing Cao, Meiling Zhang, Tengteng Xu, Zhen Chen, Xu Tong, Dandan Zhang, Yiqing Wang, Ling Zhang, Di Gao, Lei Luo, Ibrar Muhammad Khan, Yunhai Zhang
Accurate reprogramming of DNA methylation occurring in preimplantation embryos is critical for normal development of both fetus and placenta. Environmental stresses imposed on oocytes usually cause the abnormal DNA methylation reprogramming of early embryos. However, whether oocyte vitrification alters the reprogramming of DNA methylation (5 mC) and its derivatives in mouse preimplantation embryo development remains largely unknown. Here, we found that the rate of cleavage and blastocyst formation of embryos produced by IVF of vitrified matured oocytes was significantly lower than that in control counterparts, but the quality of blastocysts was not impaired by oocyte vitrification...
January 29, 2019: Cryobiology
Yan Wang, Wei-Hao Zhang, Xu Chang, Fei Cao, Hai-Peng Yu, Wen-Ge Xing, Xue-Ling Yang, Zhi Guo
OBJECTIVE: To explore the feasibility, safety and effectiveness of percutaneous cryoablation combined with systemic chemotherapy in the treatment of liver metastases from esophageal carcinoma (ECLM). MATERIALS AND METHODS: We retrospectively collected data of 16 patients who received CT-guided percutaneous cryoablation concurrent systemic chemotherapy for liver metastases after primary esophageal carcinoma resection. Functional Assessment of Cancer Therapy-General (FACT-G) was used for the assessment of quality of life (QOL), and overall survival (OS), progression-free survival (PFS) and complications were also evaluated...
January 28, 2019: Cryobiology
Cristina Álvarez, Victoria Luño, Noelia González, Lydia Gil
Sperm quality in donkeys (Equus asinus) after freezing thawing is still considered lower than that from other animals, including horses. The aim of this study was to test a new freezing extender supplemented with jenny colostrum on donkey sperm. After thawing, we evaluated sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional integrity by HOS-test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI...
January 24, 2019: Cryobiology
Hirokazu Kusakabe
Present study aimed to determine to what extent freeze-dried spermatozoa were able to withstand high-temperature conditions: transient increase in storage temperature and long-term exposure to room temperature. Mouse spermatozoa were freeze-dried in EGTA/Tris-HCl buffered solution alkalinized using KOH (K-ETBS, pH 7.7), and then stored for up to 7 months at 4 °C or 25 °C. After 2 months' storage, some of the 4°C-stored spermatozoa were exposed to 40 °C for 1 week or 1 month, then again stored at 4 °C for the remaining storage period...
January 22, 2019: Cryobiology
S P Leibo, Jorge M Sztein
In 1972, a procedure was derived to cryopreserve mouse embryos. Over the past four decades, this procedure has been adapted to freeze embryos of more than twenty-five mammalian species. Cryopreservation of embryos has become a routine procedure in both veterinary and human medicine, having been used to freeze millions of embryos of mice and cattle, and many hundreds of thousands of human embryos. After transfer into appropriate foster mothers, cryopreserved embryos have developed into innumerable live offspring...
January 21, 2019: Cryobiology
Normah M Noor, Norafarain Sulong, Barbara M Reed
There is a pressing need for practical and successful conservation efforts to establish long-term germplasm collections of recalcitrant and tropical species, given the challenge and threat that these plants are facing. Cryopreservation is the only way of conserving some of these species, especially those with temperature or desiccation sensitive (recalcitrant) seeds. This review covers reports on cryopreservation studies of shoot tips (apical and axillary) of tropical and subtropical plants. Since many of these species have recalcitrant seeds, the cryopreservation successes, failures and problems involved with these seeds are also discussed...
January 21, 2019: Cryobiology
Rasha Al-Attar, Sanoji Wijenayake, Kenneth B Storey
Wood frogs, Rana sylvatica, can undergo prolonged periods of whole body freezing during winter, locking as much as 65-70% of total body water into extracellular ice and imposing both anoxia and dehydration on their cells. Metabolic rate depression (MRD) is an adaptation used by R. sylvatica to survive these environmental stresses, where a finite amount of ATP generated through anaerobic metabolism is directed towards maintaining pro-survival functions, while most ATP-expensive cellular processes are temporarily reduced in function...
January 10, 2019: Cryobiology
Matthew J Powell-Palm, Yanfang Zhang, Justin Aruda, Boris Rubinsky
While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value...
January 7, 2019: Cryobiology
Xiao Liang, Xiang Hu, Yue Hu, Weijie Zeng, Guifang Zeng, Yuan Ren, Yuncheng Liu, Kangzhuo Chen, Hao Peng, Hailei Ding, Muyun Liu
In this study, we compared three commercially available and two widely used CPAs for their ability of cryopreserving PBMCs. Similar survival (81.0%) and recovery rate (73.7%) were observed among cells using these five CPAs. However, all the cryopreserved PBMCs exhibited a significantly lower survival rate when compared with the fresh samples (94.3%). We further evaluated effector cell subpopulation and tumoricidal activity of PBMC-derived cytokine-induced killing (CIK) cells and natural killing (NK) cells. Similar and high survival (CIK: 88...
January 7, 2019: Cryobiology
Anna Skrzek, Agnieszka Ciszek, Danuta Nowicka, Agnieszka Dębiec-Bąk
The aim of the work was to evaluate changes in selected skin parameters under the influence of low temperature. The tests were conducted on a group of 20 women using whole-body cryotherapy. The average age of participants was 58.7 ± 7.54 years; the average body weight 77.84 ± 16.01 kg, the mean BMI 30.14 ± 5.81, and the average body height 160.7 ± 6.48 cm. The tested parameters included hydration, lubrication, temperature, and pH of the skin. The skin measurements were made on the first and tenth treatment days, before and after leaving the whole-body cryo-chamber...
January 7, 2019: Cryobiology
A Arando, J V Delgado, A Fernández-Prior, J M León, A Bermúdez-Oria, S Nogales, C C Pérez-Marín
The aim of the present study was to evaluate the effect of the addition of different concentrations of two olive oil-derived antioxidants, hydroxytyrosol (3,4-dihydroxyphenylethanol, HT) and 3,4-dihydroxyphenylglycol (DHPG), on ovine semen during the freezing-thawing process. Sperm was collected, pooled and diluted with commercial extenders and then divided into aliquots supplemented with different concentrations (10 μg/ml, 30 μg/ml, 50 μg/ml and 70 μg/ml) of HT, DHPG and a mixture (MIX) of both antioxidants...
January 3, 2019: Cryobiology
P Heres, R Rodriguez-Riveiro, J Troncoso, E Paredes
Global aquaculture production of blue mussel has increased over last years. This work reaffirms the great potential of cryopreservation technique on mussel industry and overcome economic barriers a cause of a traditional and rudimentary management and continue growing. The aim of this work is to set some preliminary basis attending to toxicity of cryoprotecting agents (CPAs) on different development stages of Mytilus galloprovincialis as a start point to develop a stable cryopreservation protocol. Toxicity tests were carried out by using common CPAs (dimethyl-sulfoxide (Me2 SO), glycerol, (GLY), propylene glycol (PG) and ethylene glycol (EG)) in a range from 0...
January 3, 2019: Cryobiology
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