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BMC Microbiology

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https://read.qxmd.com/read/30782119/purification-and-characterization-of-actinomycins-from-streptomyces-strain-m7-active-against-methicillin-resistant-staphylococcus-aureus-and-vancomycin-resistant-enterococcus
#1
Manish Sharma, Rajesh Kumari Manhas
BACKGROUND: The increased rate of resistance among two highly concerned pathogens i.e. methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) necessitates the discovery of novel anti-MRSA and anti-VRE compounds. In microbial drug discovery, Streptomyces are well known source of two-thirds of natural antibiotics used clinically. Hence, screening of new strains of streptomycetes is the key step to get novel bioactive compounds with antimicrobial activity against drug resistant bacteria...
February 19, 2019: BMC Microbiology
https://read.qxmd.com/read/30777007/sensitive-and-immunogen-specific-serological-detection-of-rodentibacter-pneumotropicus-infections-in-mice
#2
Felix Fingas, Daniela Volke, Rayk Hassert, Juliane Fornefett, Sophie Funk, Christoph Georg Baums, Ralf Hoffmann
BACKGROUND: Rodentibacter (R.) pneumotropicus colonizes the respiratory and urogenital tracts of laboratory mice with a reported moderate serological prevalence from 4 to 13%. Thus, regular tests to identify this pathogen in mice are recommended for animal facilities. However, a recent study indicated that current serological assays are partly insensitive, as C57BL/6 and BALB/c mice infected with R. pneumotropicus were incorrectly screened as seronegative. RESULTS: Here, we report a systematic analysis of protein and lipopolysaccharides antigens by immunoblot and ELISA that allowed establishing a sensitive test system able to differentiate between R...
February 18, 2019: BMC Microbiology
https://read.qxmd.com/read/30777006/gut-microbiome-patterns-correlate-with-higher-postoperative-complication-rates-after-pancreatic-surgery
#3
Felix C F Schmitt, Thorsten Brenner, Florian Uhle, Svenja Loesch, Thilo Hackert, Alexis Ulrich, Stefan Hofer, Alexander H Dalpke, Markus A Weigand, Sébastien Boutin
BACKGROUND: Postoperative complications are of great relevance in daily clinical practice, and the gut microbiome might play an important role by preventing pathogens from crossing the intestinal barrier. The two aims of this prospective clinical pilot study were: (1) to examine changes in the gut microbiome following pancreatic surgery, and (2) to correlate these changes with the postoperative course of the patient. RESULTS: In total, 116 stool samples of 32 patients undergoing pancreatic surgery were analysed by 16S-rRNA gene next-generation sequencing...
February 18, 2019: BMC Microbiology
https://read.qxmd.com/read/30777002/ability-of-the-so-called-obligate-hydrocarbonoclastic-bacteria-to-utilize-nonhydrocarbon-substrates-thus-enhancing-their-activities-despite-their-misleading-name
#4
Samir S Radwan, Majida M Khanafer, Husain A Al-Awadhi
BACKGROUND: The group of the so-called obligate hydrocarbonoclastic bacteria (OHCB) are marine microorganisms affiliated with the genera Alcanivorax, Cycloclasticus, Oleiphilus and Thalassolituus. This small group plays a major role in oil-bioremediation in marine ecosystems. Marinobacter and Planomicrobium are considered related to this group. The OHCB are claimed to be obligate to hydrocarbon nutrition. This study argues against this claim. RESULTS: Four Alcanivorax species, three Marinobacter species and Planomicrobium okeanokoites from the Arabian/Persian Gulf proved to be not obligate to hydrocarbon nutrition...
February 18, 2019: BMC Microbiology
https://read.qxmd.com/read/30760216/characterization-of-enzymatic-properties-of-two-novel-enzymes-3-4-dihydroxyphenylacetate-dioxygenase-and-4-hydroxyphenylacetate-3-hydroxylase-from-sulfobacillus-acidophilus-tpy
#5
Wenbin Guo, Wengen Zhou, Hongbo Zhou, Xinhua Chen
BACKGROUND: As an environmental pollutant, 4-hydroxyphenylacetate (4-HPA) was a product of softwood lignin decomposition and was found in industrial effluents from olive oil production. Sulfobacillus acidophilus TPY was a moderately thermoacidophilic bacterium capable of degrading aromatic compounds including 4-HPA. The enzymes involved in the degradation of 4-HPA and the role of this strain in the bioremediation of marine pollutants need to be illustrated. RESULTS: 3,4-dihydroxyphenylacetate dioxygenase (DHPAO) encoded by mhpB2 and two components of 4-hydroxydroxyphenylacetate (4-HPA) 3-hydroxylase encoded by hpaB and hpaC from S...
February 13, 2019: BMC Microbiology
https://read.qxmd.com/read/30760203/inhibition-of-pathogenic-bacterial-biofilms-on-pdms-based-implants-by-l-acidophilus-derived-biosurfactant
#6
Surekha K Satpute, Nishigandha S Mone, Parijat Das, Ibrahim M Banat, Arun G Banpurkar
BACKGROUND: Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Biosurfactant (BS) obtained from Lactobacillus spp. exhibit antibiofilm and antiadhesive activity against broad range of microbes. In the present study we investigated the production, purification and properties of key components of the cell-associated-biosurfactant (CABS) from Lactobacillus acidophilus NCIM 2903...
February 13, 2019: BMC Microbiology
https://read.qxmd.com/read/30760201/mangrove-derived-streptomyces-sp-mum265-as-a-potential-source-of-antioxidant-and-anticolon-cancer-agents
#7
Loh Teng-Hern Tan, Kok-Gan Chan, Priyia Pusparajah, Wai-Fong Yin, Tahir Mehmood Khan, Learn-Han Lee, Bey-Hing Goh
BACKGROUND: Colon cancer is the third most commonly diagnosed cancer worldwide, with a commensurately high mortality rate. The search for novel antioxidants and specific anticancer agents which may inhibit, delay or reverse the development of colon cancer is thus an area of great interest; Streptomyces bacteria have been demonstrated to be a source of such agents. RESULTS: The extract from Streptomyces sp. MUM265- a strain which was isolated and identified from Kuala Selangor mangrove forest, Selangor, Malaysia- was analyzed and found to exhibit antioxidant properties as demonstrated via metal-chelating ability as well as superoxide anion, DPPH and ABTS radical scavenging activities...
February 13, 2019: BMC Microbiology
https://read.qxmd.com/read/30744555/application-of-qpcr-assays-based-on-haloacids-transporter-gene-dehp2-for-discrimination-of-burkholderia-and-paraburkholderia
#8
Xianbin Su, Yi Shi, Ruihong Li, Zhao-Ning Lu, Xin Zou, Jiao-Xiang Wu, Ze-Guang Han
BACKGROUND: A major facilitator superfamily transporter Dehp2 was recently shown to be playing an important role in transport and biodegradation of haloacids in Paraburkholderia caribensis MBA4, and Dehp2 is phylogenetically conserved in Burkholderia sensu lato. RESULTS: We designed both Burkholderia sensu stricto-specific and Paraburkholderia-specific qPCR assays based on dehp2 and 16S rRNA, and validated the qPCR assays in 12 bacterial strains. The qPCR assays could detect single species of Burkholderia sensu stricto or Paraburkholderia with high sensitivity and discriminate them in mixtures with high specificity over a wide dynamic range of relative concentrations...
February 11, 2019: BMC Microbiology
https://read.qxmd.com/read/30744550/biological-characterization-of-pasteurella-multocida-present-in-the-saiga-population
#9
Mukhit Orynbayev, Kulyaisan Sultankulova, Abylay Sansyzbay, Rashida Rystayeva, Kamshat Shorayeva, Aidar Namet, Sasan Fereidouni, Gulnaz Ilgekbayeva, Kainar Barakbayev, Syrym Kopeyev, Richard Kock
BACKGROUND: This study provides biochemical and molecular genetic characteristics of P. multocida isolated from dead saigas in 1988, 2010-2015 on the territory of the Republic of Kazakhstan. RESULTS: Bacteriological samples taken from carcasses of saiga antelope during mortality events recorded in West Kazakhstan in both 2010 and 2011 and in Kostanay in 2012 and 2015 confirmed the presence of P. multocida, according to morphological and biochemical characterisation...
February 11, 2019: BMC Microbiology
https://read.qxmd.com/read/30744547/the-study-of-metabolites-from-fermentation-culture-of-alternaria-oxytropis
#10
Runjie Song, Jinglong Wang, Lu Sun, Yajing Zhang, Zhenghui Ren, Baoyu Zhao, Hao Lu
BACKGROUND: The indolizidine alkaloid-swainsonine is produced by an endophytic fungus Alternaria oxytropis, which was isolated from locoweeds. Swainsonine has many biological activities such as anti-tumorigenic, anti-viral and bacteriostatic. However, the full complement of metabolites produced by Alternaria oxytropis is not known. This study is a chemical analysis of Alternaria oxytropis metabolites, which not only unravels the potential compounds from the fermentation broth but also in which solvent are they extracted, facilitating industrial application...
February 11, 2019: BMC Microbiology
https://read.qxmd.com/read/30736736/osteopontin-in-the-host-response-to-leishmania-amazonensis
#11
Emilie Giraud, Eline Rouault, Laurence Fiette, Jean-Hervé Colle, Despoina Smirlis, Evie Melanitou
BACKGROUND: Leishmania (L.) spp are intracellular eukaryotic parasites responsible for cutaneous or visceral leishmaniasis, replicating predominantly in macrophages (MF). In C57BL/6 mice virulence with L. amazonensis has been associated with inhibition of Th1 immune responses and an uncontrolled lesion development, whereas DBA/2 mice control any lesion. Parasitic clearance by the MFs requires the activation of proper immune responses. One of the immune related genes expressed in immune cells including MF, codes for osteopontin (OPN)...
February 8, 2019: BMC Microbiology
https://read.qxmd.com/read/30736731/lactobacillus-mucosae-dpc-6426-as-a-bile-modifying-and-immunomodulatory-microbe
#12
Paul M Ryan, Ellen H Stolte, Lis E E London, Jerry M Wells, Sarah L Long, Susan A Joyce, Cormac G M Gahan, Gerald F Fitzgerald, R Paul Ross, Noel M Caplice, Catherine Stanton
BACKGROUND: Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model. This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease. Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity. The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587...
February 8, 2019: BMC Microbiology
https://read.qxmd.com/read/30736730/single-stranded-ss-rna-mediated-antiviral-response-against-infectious-laryngotracheitis-virus-infection
#13
Mohamed Sarjoon Abdul-Cader, Upasama De Silva Senapathi, Hanaa Ahmed-Hassan, Shayan Sharif, Mohamed Faizal Abdul-Careem
BACKGROUND: Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response against infectious bursal disease virus infection. The objective of this study was to determine the innate host responses activated by the pre-hatch in ovo administration of resiquimod against infectious laryngotracheitis virus (ILTV) infection in chickens post-hatch...
February 8, 2019: BMC Microbiology
https://read.qxmd.com/read/30727944/phylogenetic-relationship-of-ornithobacterium-rhinotracheale-isolated-from-poultry-and-diverse-avian-hosts-based-on-16s-rrna-and-rpob-gene-analyses
#14
Inês M B Veiga, Dörte Lüschow, Stefanie Gutzer, Hafez M Hafez, Kristin Mühldorfer
BACKGROUND: Ornithobacterium (O.) rhinotracheale is an emerging bacterial pathogen in poultry and not fully understood to date. Because of its importance particularly for the global turkey meat industry, reliable diagnostic and characterization methods are needed for early treatment and in future for better vaccine production. The host range of birds infected by O. rhinotracheale or carrying the bacterium in their respiratory tract has constantly increased raising important epidemiological and taxonomic questions for a better understanding of its diversity, ecology and transmission cycles...
February 6, 2019: BMC Microbiology
https://read.qxmd.com/read/30717674/ruminal-metagenomic-analyses-of-goat-data-reveals-potential-functional-microbiota-by-supplementation-with-essential-oil-cobalt-complexes
#15
Zhaomin Lei, Ke Zhang, Chao Li, Ting Jiao, Jianping Wu, Yubing Wei, Kechuan Tian, Chong Li, Defu Tang, Delmer I Davis, David P Casper, Hui Jiang, Xiaolong Wang, Jianfu Wang
BACKGROUND: Essential Oils (EO) are complex mixtures of plant secondary metabolites that have been proposed as promising feed additives for mitigating methane and ammonia emissions. We have previously demonstrated that Essential Oil-Cobalt (EOC) supplementation resulted in increased average daily gain and improved phenotypes (cashmere fiber traits, carcass weight, and meat quality) when cashmere goats received supplementation at approximately 2 mg/kg of body weight. However, the ruminal microbiological effects of EO remain poorly understood with regard to the extent to which ruminal populations can adapt to EO presence as feed ingredients...
February 4, 2019: BMC Microbiology
https://read.qxmd.com/read/30710998/investigation-of-multidrug-resistant-st2-acinetobacter-baumannii-isolated-from-saint-george-hospital-in-lebanon
#16
Tania Nawfal Dagher, Charbel Al-Bayssari, Selma Chabou, Nadine Antar, Seydina M Diene, Eid Azar, Jean-Marc Rolain
BACKGROUND: Acinetobacter baumannii is an opportunistic pathogen causing various nosocomial infections. The spread of multidrug-resistant A. baumannii is a major public health problem. The aim of this study was to investigate the molecular epidemiology and the genetic support of multidrug-resistant A. baumannii isolates collected from Saint-Georges Hospital in Lebanon. METHODS: Between January and August 2016, 31 A. baumannii isolates were collected from sputum samples of patients infected with ventilator-associated pneumonia (VAP) and treated with colistin-carbapenem combination therapy...
February 2, 2019: BMC Microbiology
https://read.qxmd.com/read/30709334/ngs-based-phylogeny-of-diphtheria-related-pathogenicity-factors-in-different-corynebacterium-spp-implies-species-specific-virulence-transmission
#17
Alexandra Dangel, Anja Berger, Regina Konrad, Andreas Sing
BACKGROUND: Diphtheria toxin (DT) is produced by toxigenic strains of the human pathogen Corynebacterium diphtheriae as well as zoonotic C. ulcerans and C. pseudotuberculosis. Toxigenic strains may cause severe respiratory diphtheria, myocarditis, neurological damage or cutaneous diphtheria. The DT encoding tox gene is located in a mobile genomic region and tox variability between C. diphtheriae and C. ulcerans has been postulated based on sequences of a few isolates. In contrast, species-specific sequence analysis of the diphtheria toxin repressor gene (dtxR), occurring both in toxigenic and non-toxigenic Corynebacterium species, has not been done yet...
February 1, 2019: BMC Microbiology
https://read.qxmd.com/read/30704407/isolation-and-in-vitro-antimicrobial-susceptibility-of-porcine-lawsonia-intracellularis-from-brazil-and-thailand
#18
Suphot Wattanaphansak, Carlos Eduardo Real Pereira, Wenika Kaenson, Pornchalit Assavacheep, Rachod Tantilertcharoen, Talita Pilar Resende, Javier Alberto Barrera-Zarate, Juliana Saes Vilaça de Oliveira-Lee, Ulrich Klein, Connie Jane Gebhart, Roberto Maurício Carvalho Guedes
BACKGROUND: Lawsonia intracellularis is an obligate intracellular bacterium which cannot be cultured by conventional bacteriological methods. Furthermore, L. intracellularis needs enriched medium and a unique atmosphere for isolation, cultivation and propagation. Because of this,there are only a few isolates of L. intracellularis available and few studies in vitro demonstrating the susceptibility of this bacterium to antimicrobial agents. The objectives of this study were to isolate South American and Southeast Asia strains of L...
January 31, 2019: BMC Microbiology
https://read.qxmd.com/read/30691408/phylogenetic-relationship-of-prophages-is-affected-by-crispr-selection-in-group-a-streptococcus
#19
Shunsuke Yamada, Masaki Shibasaki, Kazunori Murase, Takayasu Watanabe, Chihiro Aikawa, Takashi Nozawa, Ichiro Nakagawa
BACKGROUND: Group A Streptococcus (GAS) is a major human pathogen, which is associated with a wide spectrum of invasive diseases, such as pharyngitis, scarlet fever, rheumatic fever, and streptococcal toxic shock syndrome (STSS). It is hypothesized that differences in GAS pathogenicity are related to the acquisition of diverse bacteriophages (phages). Nevertheless, the GAS genome also harbors clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (cas) genes, which play an important role in eliminating foreign DNA, including those of phages...
January 28, 2019: BMC Microbiology
https://read.qxmd.com/read/30691388/mfd-protects-against-oxidative-stress-in-bacillus-subtilis-independently-of-its-canonical-function-in-dna-repair
#20
Holly Anne Martin, Katelyn E Porter, Carmen Vallin, Tatiana Ermi, Natalie Contreras, Mario Pedraza-Reyes, Eduardo A Robleto
BACKGROUND: Previous reports showed that mutagenesis in nutrient-limiting conditions is dependent on Mfd in Bacillus subtilis. Mfd initiates one type of transcription-coupled repair (TCR); this type of repair is known to target bulky lesions, like those associated with UV exposure. Interestingly, the roles of Mfd in repair of oxidative-promoted DNA damage and regulation of transcription differ. Here, we used a genetic approach to test whether Mfd protected B. subtilis from exposure to two different oxidants...
January 28, 2019: BMC Microbiology
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