journal
https://read.qxmd.com/read/37771302/transplantation-of-cryopreserved-olfactory-ensheathing-cells-restores-loss-of-functions-in-an-experimental-model
#1
JOURNAL ARTICLE
Kamile Minkelyte, Daqing Li, Ying Li, Ahmed Ibrahim
In the past decades, the properties of olfactory ensheathing cells (OECs) have been widely investigated. Studies have shown that transplantation of OECs cultured from the olfactory bulb mediates axonal regeneration, remyelination and restores lost functions in experimental central nervous system (CNS) injury models. Autologously sourcing the cells from the nasal mucosa or the olfactory bulb to treat patients with spinal cord injuries would be ideal, but the cell yield achieved may be inadequate to cover the surface area of the lesions typically encountered in human spinal cord contusion injuries...
2023: Cell Transplantation
https://read.qxmd.com/read/37766590/rejuvenation-of-senescent-mesenchymal-stem-cells-to-prevent-age-related-changes-in-synovial-joints
#2
REVIEW
Tatiana Sekelova, Lubos Danisovic, Michaela Cehakova
Mesenchymal/medicinal stem/signaling cells (MSCs), well known for regenerative potential, have been involved in hundreds of clinical trials. Even if equipped with reparative properties, aging significantly decreases their biological activity, representing a major challenge for MSC-based therapies. Age-related joint diseases, such as osteoarthritis, are associated with the accumulation of senescent cells, including synovial MSCs. An impaired ability of MSCs to self-renew and differentiate is one of the main contributors to the human aging process...
2023: Cell Transplantation
https://read.qxmd.com/read/37737125/egr1-promotes-ovarian-hyperstimulation-syndrome-through-upregulation-of-sox9-expression
#3
JOURNAL ARTICLE
Huihui Wang, Weijia Chen, Yinan Huang, Yuan Sun, Yuanhua Liu, Yuanchao Zhu, Zongxuan Lu
Angiogenesis is strongly associated with ovarian hyperstimulation syndrome (OHSS) progression. Early growth response protein 1 (EGR1) plays an important role in angiogenesis. This study aimed to investigate the function and mechanism of EGR1 involved in OHSS progression. RNA-sequencing was used to identify differentially expressed genes. In vitro OHSS cell model was induced by treating KGN cells with human chorionic gonadotropin (hCG). In vivo OHSS model was established in mice. The expression levels of EGR1, SOX1, and VEGF were determined by Quantitative Real-Time polymerase chain reaction (qRT-PCR), Western blot, immunofluorescence staining, and immunochemistry assay...
2023: Cell Transplantation
https://read.qxmd.com/read/37724837/establishing-an-efficient-electroporation-based-method-to-manipulate-target-gene-expression-in-the-axolotl-brain
#4
JOURNAL ARTICLE
Sulei Fu, Cheng Peng, Yan-Yun Zeng, Yuanhui Qiu, Yanmei Liu, Ji-Feng Fei
The tetrapod salamander species axolotl ( Ambystoma mexicanum ) is capable of regenerating injured brain. For better understanding the mechanisms of brain regeneration, it is very necessary to establish a rapid and efficient gain-of-function and loss-of-function approaches to study gene function in the axolotl brain. Here, we establish and optimize an electroporation-based method to overexpress or knockout/knockdown target gene in ependymal glial cells (EGCs) in the axolotl telencephalon. By orientating the electrodes, we were able to achieve specific expression of EGFP in EGCs located in dorsal, ventral, medial, or lateral ventricular zones...
2023: Cell Transplantation
https://read.qxmd.com/read/37724822/research-for-cytomegalovirus-mutations-associated-with-resistance-to-antivirals-in-kidney-transplant-receptors
#5
LETTER
Carla de Castro Sant' Anna, Silvia Regina da Cruz Migone, Carlos Alberto Machado da Rocha, Fernando Augusto Rodrigues Mello Júnior, Aline Damasceno Seabra, Thaís Brilhante Pontes, João Marildo Rodrigues, Salomé Aparecida Soares, Viviane de Paiva Rego, Joana Paula Figueira, Ana Paula Monteiro Rodrigues, Rommel Mario Rodriguez Burbano
Cytomegalovirus (CMV) mutations associated with antiviral resistance have become a major problem related to high mortality in kidney transplant patients. The aim of the study was to investigate mutations in the CMV genes UL97 and UL54 associated with antiviral resistance. A retrospective observational cohort study was carried out at Hospital Ophir Loyola (HOL), a reference in Kidney Transplantation. A total of 81 patients who underwent kidney transplantation were followed up between 2016 and 2018 were monitored for CMV viral load by performing qPCR...
2023: Cell Transplantation
https://read.qxmd.com/read/37710973/periodontal-tissue-regeneration-by-transplantation-of-autologous-adipose-tissue-derived-multi-lineage-progenitor-cells-with-carbonate-apatite
#6
JOURNAL ARTICLE
Masahide Takedachi, Kohsuke Kawasaki, Keigo Sawada, Kazuma Sakura, Mari Murata, Junpei Shimomura, Kazuma Kawakami, Chiaki Morimoto, Koji Miki, Noboru Takeshita, Tomoaki Iwayama, Hanayuki Okura, Akifumi Matsuyama, Masahiro Saito, Masahiro Kitamura, Shinya Murakami
We have developed an autologous transplantation method using adipose tissue-derived multi-lineage progenitor cells (ADMPCs) as a method of periodontal tissue regeneration that can be adapted to severe periodontal disease. Our previous clinical study confirmed the safety of autologous transplantation of ADMPCs and demonstrated its usefulness in the treatment of severe periodontal disease. However, in the same clinical study, we found that the fibrin gel used as the scaffold material might have caused gingival recession and impaired tissue regeneration in some patients...
2023: Cell Transplantation
https://read.qxmd.com/read/37706453/haploidentical-natural-killer-cell-therapy-as-an-adjunct-to-stem-cell-transplantation-for-treatment-of-refractory-acute-myeloid-leukemia
#7
JOURNAL ARTICLE
Uday Kulkarni, Arun Kumar Arunachalam, Hamenth Kumar Palani, Reeshma Radhakrishnan Nair, Nithya Balasundaram, Arvind Venkatraman, Anu Korula, Sushil Selvarajan, Sharon Lionel, Poonkuzhali Balasubramanian, Madhavi Maddali, Aby Abraham, Biju George, Vikram Mathews
Refractory acute myeloid leukemia (AML), defined as failure of two cycles of induction therapy at diagnosis or of one cycle at relapse, represents a subgroup with poor outcomes. Haploidentical natural killer cell (NK) therapy is a strategy that is being explored in refractory malignancies. Historically, at our center, patients with refractory AML have been treated with cytoreductive therapy (fludarabine + cytosine + granulocyte colony-stimulating factor ± idarubicin or mitoxantrone + etoposide) followed by 1-week rest and then reduced-intensity transplant with fludarabine + melphalan...
2023: Cell Transplantation
https://read.qxmd.com/read/37706441/role-of-serum-inflammatory-cytokines-in-sepsis-rats-following-bmscs-transplantation-protein-microarray-analysis
#8
JOURNAL ARTICLE
Guanghui Xiu, Xiuling Li, Juan Chen, Jintao Li, Kun Chen, Ping Liu, Bin Ling, Ying Yang
Bone marrow stromal cells (BMSCs) have emerged as a potential therapy for sepsis, yet the underlying mechanisms remain unclear. In this study, we investigated the effects of BMSCs on serum inflammatory cytokines in a rat model of lipopolysaccharide (LPS)-induced sepsis. Sepsis was induced by intravenous injection of LPS, followed by transplantation of BMSCs. We monitored survival rates for 72 h and evaluated organ functions, histopathological changes, and cytokines expression. Sepsis rats showed decreased levels of white blood cells, platelets, lymphocyte ratio, and oxygen partial pressure, along with increased levels of neutrophil ratio, carbon dioxide partial pressure, lactic acid, alanine aminotransferase, and aspartate aminotransferase...
2023: Cell Transplantation
https://read.qxmd.com/read/37698258/coating-free-culture-medium-for-establishing-and-maintaining-human-induced-pluripotent-stem-cells
#9
JOURNAL ARTICLE
Chih-Yao Lin, Yu-Yun Ching, Shih-Fang Wu, Yi-Ko Lee, Hueng-Chuen Fan, Liang-Yu Su, Su-Yi Tsai, Yu-Ching Chen, Ching-I Shen, Hong-Lin Su
Cell expansion of human pluripotent stem cells (hPSCs) commonly depends on Matrigel as a coating matrix on two-dimensional (2D) culture plates and 3D microcarriers. However, the xenogenic Matrigel requires sophisticated quality-assurance processes to meet clinical requirements. In this study, we develop an innovative coating-free medium for expanding hPSCs. The xenofree medium supports the weekend-free culture and competitive growth of hPSCs on several cell culture plastics without an additional pre-coating process...
2023: Cell Transplantation
https://read.qxmd.com/read/37698245/repairing-mechanisms-for-distal-airway-injuries-and-related-targeted-therapeutics-for-chronic-lung-diseases
#10
REVIEW
Huahua Xu, Guihong Pan, Jun Wang
Chronic lung diseases, such as chronic obstructive pulmonary disease (COPD) and idiopathic pulmonary fibrosis (IPF), involve progressive and irreversible destruction and pathogenic remodeling of airways and have become the leading health care burden worldwide. Pulmonary tissue has extensive capacities to launch injury-responsive repairing programs (IRRPs) to replace the damaged or dead cells upon acute lung injuries. However, the IRRPs are frequently compromised in chronic lung diseases. In this review, we aim to provide an overview of somatic stem cell subpopulations within distal airway epithelium and the underlying mechanisms mediating their self-renewal and trans-differentiation under both physiological and pathological circumstances...
2023: Cell Transplantation
https://read.qxmd.com/read/37688441/injectable-decorin-gellan-gum-hydrogel-encapsulating-adipose-derived-stem-cells-enhances-anti-inflammatory-effect-in-cartilage-injury-via-autophagy-signaling
#11
JOURNAL ARTICLE
Weiping He, Yu Wu, Zhihong Luo, Genghua Yang, Woquan Ye, Xi Chen, Jianhua Ren, Tangzhao Liang, Zhiqiang Liao, Shihai Jiang, Kun Wang
Adipose-derived stem cells (ADSCs) are employed as a promising alternative in treating cartilage injury. Regulating the inflammatory "fingerprint" of ADSCs to improve their anti-inflammatory properties could enhance therapy efficiency. Herein, a novel injectable decorin/gellan gum hydrogel combined with ADSCs encapsulation for arthritis cartilage treatment is proposed. Decorin/gellan gum hydrogel was prepared according to the previous manufacturing protocol. The liquid-solid form transition of gellan gum hydrogel is perfectly suitable for intra-articular injection...
2023: Cell Transplantation
https://read.qxmd.com/read/37667507/hla-fully-mismatched-sibling-derived-cd7-car-t-therapy-bridging-to-haploidentical-hematopoietic-stem-cell-transplantation-for-hepatosplenic-%C3%AE-%C3%AE-t-cell-lymphoma
#12
JOURNAL ARTICLE
Xueer Xu, Cheng Zu, Mingming Zhang, Pingnan Xiao, Ruimin Hong, Jingjing Feng, Huijun Xu, Jiazhen Cui, Jian Yu, Jimin Shi, Guoqing Wei, Alex H Chang, He Huang, Yongxian Hu
While chimeric antigen receptor (CAR)-T-cell therapy has demonstrated remarkable effectiveness in the treatment of B-cell lymphomas and leukemias, research on T-cell malignancies is still limited. Here, we reported a patient with hepatosplenic γδ T-cell lymphoma refractory to multiple lines of chemotherapy, who eventually achieved first complete remission with flow cytometry-confirmed minimal residual disease negativity after human leukocyte antigen (HLA) fully-mismatched sibling-derived CD7 CAR-T therapy...
2023: Cell Transplantation
https://read.qxmd.com/read/37650431/aligning-the-cord-blood-bone-marrow-differences-and-the-post-transplantation-clinical-manifestations-by-single-cell-rna-seq
#13
JOURNAL ARTICLE
Jiali Wang, Yaping Wang, Yong Xu, Wenfeng Fu, Liucheng Rong, Yao Xue, Yongjun Fang
Cord blood (CB) transplantation is a promising treatment for hematologic malignancies due to its strong graft-versus-leukemia effect and a low incidence of graft-versus-host disease. However, the risk of infection caused by delayed engraftment has limited its clinical application. In this study, we compared the single-cell RNA-seq of CB, bone marrow (BM), and granulocyte colony-stimulating factor primed BM to understand the differences between these grafts from a comprehensive view, and verified some differences in our clinical data of patients receiving transplantation...
2023: Cell Transplantation
https://read.qxmd.com/read/37650419/a-machine-learning-analysis-of-prognostic-genes-associated-with-allograft-tolerance-after-renal-transplantation
#14
JOURNAL ARTICLE
Zhibiao Li, Zechao Lu, Chuxian Hu, Yixin Zhang, Yushu Chen, Jiahao Zhang, Feng Guo, Jinjin Wang, Zhicheng Tang, Fucai Tang, Zhaohui He
In this study, we aimed to identify transplantation tolerance (TOL)-related gene signature and use it to predict the different types of renal allograft rejection performances in kidney transplantation. Gene expression data were obtained from the Gene Expression Omnibus (GEO) database, differently expressed genes (DEGs) were performed, and the gene ontology (GO) function enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were also conducted. The machine learning methods were combined to analyze the feature TOL-related genes and verify their predictive performance...
2023: Cell Transplantation
https://read.qxmd.com/read/37646153/mild-acute-graft-versus-host-disease-improves-outcomes-after-hla-haploidentical-related-donor-transplantation-using-posttransplant-cyclophosphamide-and-cord-blood-transplantation
#15
JOURNAL ARTICLE
Fumiya Wada, Junya Kanda, Kimimori Kamijo, Masashi Nishikubo, Satoshi Yoshioka, Takayuki Ishikawa, Yasunori Ueda, Takashi Akasaka, Yasuyuki Arai, Kiyotaka Izumi, Hirokazu Hirata, Takashi Ikeda, Akihito Yonezawa, Naoyuki Anzai, Mitsumasa Watanabe, Kazunori Imada, Kazuhiro Yago, Naoki Tamura, Mitsuru Itoh, Yuki Masuo, Akane Kunitomi, Tomoharu Takeoka, Toshiyuki Kitano, Nobuyoshi Arima, Masakatsu Hishizawa, Kohsuke Asagoe, Tadakazu Kondo, Akifumi Takaori-Kondo
Haploidentical-related donor transplantation using posttransplant cyclophosphamide (PTCy-haplo) and cord blood transplantation (CBT) are valid alternatives for patients with hematological malignancies when HLA-matched donor transplantation (MDT) is unavailable. However, the effects of graft-versus-host disease (GVHD) on outcomes after these transplants have not been fully elucidated. Therefore, we evaluated the effects of acute and chronic GVHD on transplant outcomes after PTCy-haplo transplants and compared them with CBT and MDT...
2023: Cell Transplantation
https://read.qxmd.com/read/37644776/comparative-analysis-of-the-results-of-stroke-treatment-with-multiple-administrations-of-wharton-s-jelly-mesenchymal-stem-cells-derived-he-atmp-and-standard-conservative-treatment-case-series-study
#16
JOURNAL ARTICLE
Olga Milczarek, Jakub Swadźba, Patrycja Swadźba, Anna Starowicz-Filip, Roger M Krzyżewski, Stanisław Kwiatkowski, Marcin Majka
Stroke remains still the leading cause of long-term disability worldwide. Although interventions such as early reperfusion, intravenous thrombolysis, and endovascular revascularization have shown neurological benefit in stroke patients, there is still lack of effective treatment enabling regeneration of nervous tissue after cerebral ischemic episodes. Cell therapy is an evolving opportunity for stroke survivors with residual neurological deficits. The purpose of this study was to evaluate safety and potential efficacy of multiple administration of Hospital Exemption-Advanced Therapy Medicinal Product (HE-ATMP) comprising 3 × 107 Wharton's jelly mesenchymal stem cells (WJMSCs)...
2023: Cell Transplantation
https://read.qxmd.com/read/37632354/the-role-and-applications-of-exosomes-in-gynecological-cancer-a-review
#17
REVIEW
Kai-Hung Wang, Dah-Ching Ding
Exosomes are phospholipid bilayer vesicles that are released by all types of cells, containing proteins, lipids, and nucleic acids such as DNAs and RNAs. Exosomes can be transferred between cells and play a variety of physiological and pathological regulatory functions. Noncoding RNAs, including micro RNAs, long noncoding RNAs, and circular RNAs, are the most studied biomolecules from exosomes and more and more studies found that noncoding RNAs play an important role in the diagnosis, prognosis, and treatment of diseases, including various types of cancer...
2023: Cell Transplantation
https://read.qxmd.com/read/37632352/whole-blood-derived-circusp10-acts-as-a-diagnostic-biomarker-in-patients-with-early-stage-non-small-cell-lung-cancer
#18
JOURNAL ARTICLE
Huihui Bai, Meina Jiang, Shuai Fang, Ziyi Peng, Nan Liang, Yuanting Cai, Yuanyuan Wang, Chengwei Zhou, Ying Han, Weiyu Shen, Zhaohui Gong
Accumulating evidence has indicated that differentially expressed noncoding circular RNAs (circRNAs) play essential roles in the occurrence and development of various types of cancer. Here, we aimed to identify and explore the diagnostic value of hsa_circ_0003026 (named circUSP10) in patients with early non-small-cell lung cancer (NSCLC). The differentially expressed circRNAs were screened from the microarray-based assay of human NSCLC tissues and their corresponding noncancerous tissues, and the candidate circRNAs were further verified in patients with NSCLC using reverse transcription-quantitative polymerase chain reaction (RT-qPCR)...
2023: Cell Transplantation
https://read.qxmd.com/read/37615293/human-cord-blood-endothelial-progenitor-cells-alleviate-intimal-hyperplasia-of-arterial-damage-in-a-rat-stroke-model
#19
JOURNAL ARTICLE
Hongming Sun, Ryuta Morihara, Tian Feng, Zhihong Bian, Haibo Yu, Xiao Hu, Xinran Hu, Yuting Bian, Ryo Sasaki, Yusuke Fukui, Mami Takemoto, Taijun Yunoki, Yumiko Nakano, Koji Abe, Toru Yamashita
Human cord blood-endothelial progenitor cells (hCB-EPCs) isolated from the human umbilical cord can be used to repair damaged arteries. In this study, we used an animal model with pathological changes that mimics artery wall damage caused by stent retrievers in humans. We injected hCB-EPCs to investigate their effect on endothelial hyperplasia and dysfunction during intimal repair. Four groups were established based on the length of reperfusion (3 and 28 days), as well as the presence or absence of hCB-EPC therapy...
2023: Cell Transplantation
https://read.qxmd.com/read/37606168/mettl3-promotes-the-growth-and-invasion-of-melanoma-cells-by-regulating-the-lncrna-snhg3-mir-330-5p-axis
#20
JOURNAL ARTICLE
Shaojun Chu, Yulong Li, Baojin Wu, Guo Rong, Qiang Hou, Qin Zhou, Dexiang Du, Yufei Li
Accumulating evidence indicates that m6 A methyltransferase 3 (METTL3) plays a pivotal role in different malignancies including melanoma. However, the function and underlying mechanisms by which METTL3 contributes to the tumorigenesis of melanoma remain undocumented. The association of METTL3 and long noncoding RNA (lncRNA) small nucleolar RNA host gene 3 (SNHG3) with clinicopathological characteristics and prognosis in patients with melanoma was analyzed by real-time quantitative polymerase chain reaction (RT-qPCR), Western blotting, and The Cancer Genome Atlas data sets...
2023: Cell Transplantation
journal
journal
20284
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.