Martin R Späth, K Johanna R Hoyer-Allo, Lisa Seufert, Martin Höhne, Christina Lucas, Theresa Bock, Lea Isermann, Susanne Brodesser, Jan-Wilm Lackmann, Katharina Kiefer, Felix C Koehler, Katrin Bohl, Michael Ignarski, Petra Schiller, Marc Johnsen, Torsten Kubacki, Franziska Grundmann, Thomas Benzing, Aleksandra Trifunovic, Marcus Krüger, Bernhard Schermer, Volker Burst, Roman-Ulrich Müller
BACKGROUND: Clinical practice lacks strategies to treat acute kidney injury (AKI). Interestingly, preconditioning by hypoxia (HP) and caloric restriction (CR) is highly protective in rodent AKI models. However, the underlying molecular mechanisms of this process are unknown. METHODS: CRISPR generated kynureninase (KYNU) knockout mice and comparative transcriptome, proteome, and metabolite analyses of murine kidneys (C57Bl6N wild type and KYNU-deficient) pre- and post-ischemia-reperfusion injury, in the context of CR or ad libitum diet, performed...
February 9, 2023: Journal of the American Society of Nephrology: JASN